from whole monoclonal antibodies to single domain antibodies think small
Báo cáo khoa học: Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen doc
... reported the isolation of singledomain antibodies (sdAbs) consisting of the variable domain of these heavy chain antibodies, also named VHH [2] These minimal antibody domains are endowed with ... of VH domains, which allow them to bind otherwise difficult -to- reach epitopes within the cavities on the antigen surface Consequently, these fragments can recognize epitopes inaccessible to conventional ... affinity for sCEA, with a KD ranging from to 32 nm Specificity analysis by flow cytometry Flow cytometry was used to determine whether the selected sdAbs specifically bind to CEA+, but not Fig Amino acid...
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Báo cáo toán học: " Inert coupling of IRDye800CW to monoclonal antibodies for clinical optical imaging of tumor targets" potx
... for single photon emission computed tomography [SPECT] or positron-emission tomography [PET] and are particularly well suited for a whole- body quantitative imaging of deep-seated tissues To this ... for photoimmunoconjugates This made us decide to use 89 Zr-labeled mAbs as a starting point to facilitate analytical procedures to study, analyze, and quantify the inertness of dye coupling to mAbs ... 280 nm to measure mAb absorption, at A = 430 nm to measure the absorption of N-sucDf-Fe (III), and at A = 780 nm to measure the absorption of IRDye800CW The chelate -to- mAb and IRDye800CWto-mAb...
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Báo cáo khoa học: "The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)" pdf
... and Hamilton, M J 1995 Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules, p 149-167 In: W C Davis (ed.), Monoclonal Antibody Protocols, ... similar or identical to those in cattle The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis) Table Monoclonal antibodies reactive ... crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis) development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte...
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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx
... Hamilton MJ Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules In: Davis WC (ed.) Monoclonal Antibody Protocols pp 149-167, Humana Press, Totowa, ... Totowa, 1995 12 Davis WC, Drbal K, Mosaad AE, Elbagory AR, Tibary A, Barrington GM, Park YH, Hamilton MJ Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes ... varied from six months to about two years Preparation of leukocytes for flow cytometry Because of the tendency for T lymphocytes to bind to erythrocytes, separation medium could not be used to isolate...
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Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx
... light chains derived from a range of human antibodies One of these antibodies, IS4, is an IgG antibody derived from a primary APS patient IS4 binds to anionic phospholipid antibodies only in the ... chain/light chain combinations to CL was tested by direct ELISA Materials and methods Human monoclonal antibodies IS4, B3 and UK4 are all human IgG monoclonal antibodies produced from lymphocytes of three ... phospholipid antibodies and DNA [21] The introduction of arginine residues into the VH at positions known to mediate DNA binding enhanced binding to phosphatidylserine–β2GPI complexes and to apoptotic...
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Tài liệu Báo cáo khoa học: Chromatin under mechanical stress: from single 30 nm fibers to single nucleosomes pdf
... DNA from the histone octamer, mainly from contacts with histones H2A ⁄ H2B where the DNA–histone interactions are supposed to be weak [42] The total amount of DNA released was calculated to be ... released lengths to a single nucleosomal DNA unwrapping, however, appeared not to be straightforward Upon eviction of the histone octamer and histone H1 (i.e the disruption of the chromatosome) 166 ... and a histone octamer, is complemented with linker histone (H1) and an additional 20 DNA bp to form the chromatosome Linker DNA completes and links consecutive nucleosomes which fold into the 30-nm...
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Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx
... Protocol 1, (b) protocol 2, (c) protocol distinct epitope clusters to which one or more of the mAbs bound Nine representative mAbs, each reacting with one of the ve clusters, were subjected to ... study, [16]) supplemented the epitope mapping and provided a deeper insight into operation of the TC domains The binding of TCặCbl to the receptor was suppressed by many antibodies, as well as by heparin ... be evaluated against all antibodies, however, the epitope specicity of this mAb is similar to 1-12 and Q2-12 Results Epitope mapping using surface plasmon resonance Epitope specicity was characterized...
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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
... Biochem 270) Monoclonal antibodies General To further elucidate the influence of CCP2 on protein S binding, we made the effort to raise monoclonal antibodies against CCP1-2 with the aim to use them ... that access to their epitopes was sterically hindered in the fully assembled C4BP molecule All antibodies were IgG, MoAb 15 belonged to IgG1 and MoAb 44 belonged to IgG2a class The antibodies ... were compared For both antibodies, the binding of D66A and R60A/R101A was greatly weakened as compared to wildtype suggesting the binding epitope for the monoclonal antibodies to be located in CCP2,...
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Báo cáo khoa học: Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated a subunit of the heterotrimeric GTPase Gq potx
... indicate that the C1, C2 and C3 domains must coordinate in the cytoplasm 2706 for the cytotoxicity to occur, although the enzymatic action is attributable to the C3 domain per se (c) C-PMT deamidated ... After screening with ELISA to detect antibodies specific to MUT Gq-peptide, two hybridoma cell lines producing monoclonal antibodies, 3F6 and 3G3, were established These antibodies recognized the ... multocida toxin is a mitogen for bone cells in primary culture Infect Immun 64, 959–965 Rozengurt E, Higgins T, Chanter N, Lax AJ & Staddon JM (1990) Pasteurella multocida toxin: potent mitogen...
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Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... compilation ª 2007 FEBS 1267 DNA single- strand binding to the cold shock domain K E A Max et al A Fig Comparison of open (domainswapped) and closed states of Bc-Csp (A) Torsion angle distribution of ... 141 ± 4° for Glu36w (similar to state 1) and )85 ± 1° for Gly37/ (similar to state 2) (B) Superpositions of L34 residues from Gln34 to Lys39 involving all backbone atoms (Left) Superposition of ... maintains Glu36w ⁄ Gly37/ torsion angles according to state 1, whereas the other molecule adopts a conformation according to state All other torsion angles in Bc-Csp represent single states with standard...
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Báo cáo khoa học: Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of monoclonal antibodies directed against the MalK subunit pot
... kit from Bio-Rad SDS/PAGE and immunoblot analyses were performed as described in [44] RESULTS Monoclonal antibodies recognize epitopes in the N-terminal (ATPase) domain and in the C-terminal domain ... recognize epitopes in the N-terminal (ATPase) domain while the remaining four mAbs, obtained with intact MalK, bind to the C-terminal (regulatory) domain For precise determination of the epitopes overlapping ... values correspond to approximately half of the routinely measured activities due to the removal of dithiothreitol from the buffer by dialysis in order to avoid dissociation of the antibodies) PLS,...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... 2009, 2:8 MOVCAR tumors The antibodies identified in the current study can therefore be used to monitor tumor progression in mice These antibodies can also be used to purify Muc16 so that its ... OVCAR-3, SKOV-3, and CAOV-3 were purchased from ATCC RT-PCR Total RNA was isolated from MOVCAR cell lines using the Qiagen RNeasy® Mini kit and μg of total RNA was reverse transcribed PCR reactions ... Journal of Ovarian Research 2009, 2:8 ConA Chromatography Concentrated harvest media from MOVCAR-2 cells containing 22 mg of total protein was loaded onto a mL Concanavalin A (ConA) column (Sigma;...
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Báo cáo khoa học: "Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies" docx
... G Topographical and functional mapping of epitopes on hog cholera virus with monoclonal Characterization of Antigenic Sites on the Rinderpest Virus N Protein Uusing Monoclonal Antibodies 65 antibodies ... These sites were so close to each other as the binding of an antibody to one of the epitopes can be sterically hindered by the binding of another antibody to the other epitope (steric hindrance), ... unlikely to contribute to the conformation of antigenic sites, considering that SDS breaks up disulfide bonds on the protein under mercaptoethanol The binding of all Mab except R-3E-03 to whole...
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Báo cáo khoa học: "Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)" ppt
... dessessop gI deifirup-A nietorp tsniaga desiar sbAM ehT epyt ngissa ot syassa no desab sessalc epytosi MgI ro 1GgI ,2GgI eht ot degnoleb decudorp sbAM llA )nwohs ton atad( A nietorp htiw nmuloc GgI ... gnisu eliforp tolbonummi ED-2 no niahc yvaeh nilubolgonummI fo noitacifitnedI mures eht nihtiw epytosi elgnis a sa tsixe ot thguoht si hsifkcor kcalb fo gI eht ,sbAM htiw syassa tolbonummi dna ... bAM 12 fo noitceles dewolla ,syassa tolbonummi EGAP-SDS yb dewollof ,gnineercs ASILE ]9[ debircsed ylsuoiverp sa ,nmuloc ytiniffa A nietorp eht morf deifirup nietorp ekil-gI eht htiw dezinummi ecim...
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Báo cáo khoa học: "The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin" pptx
... control) prior to addition to the MDCK cells (105) The percentage adhesion was determined relative to the attachment of untreated Leptospira onto the MDCK cells (D) Binding of Leptospira to immobilize ... (negative control) prior to addition to the MDCK cells (106) Un-treated Leptospira was used as a negative control The binding of Leptospira to ECMs or Fn or the adhesion of Leptospira to the MDCK cells ... observed to block the attachment of Leptospira, by approximately 47%, when the Fn treated Leptospira were added to the MDCK cells (Fig 1E) Thus, Fn appears to mediate the attachment of Leptospira to...
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Báo cáo y học: "Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia" ppt
... immunohistochemical tests (IHC) which indirectly detect aberrant cytoplasmic accumulation of NPM1 proteins can serve as a surrogate to molecular studies [16-18] To popularize IHC detection of cytoplasmic ... gene According to the published sequence of the NPM-mA in GenBank (no.AY740634), a pair of specific primers were designed to amplify the ORF of NPM-mA gene from pEGFP-C1-NPM-mA vectors, which were ... were cloned into the BamH I and Hind III site of the pET-32a plasmids creating fusion vectors pET-32a-NPM-mA in the presence of T4 DNA Ligase (TaKara, Tokyo, Japan) The fusion vectors were subsequently...
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Báo cáo y học: "Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on κ α NF-κB binding activity and TNF-α secretion of T cell" pot
... antiCD4 monoclonal antibody Transplantation 1992, 54:959-962 15 Espevik T, Nissen-Meyer J: A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from ... increased DTH to antigens like tetanus toxoid in less active versus active rheumatoid arthritis [25], iii) the reduced responsiveness to polyspecific stimulation of spleen cells from rats with ... àg/ml streptomycin (thereafter called R10F, all from GIBCO-BRL, Eggenstein, Germany) The resulting total Tcell suspension was 95% CD3+, as evaluated by flow cytometry Either purified total T cells...
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Báo cáo y học: "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity" pps
... C2B-16 to the mAbs (CII-6, CII-3, and C2B-14) to test the arthritogenicity We predicted that C2A-12 would exacerbate the arthritis because its ability to activate complement and bind to mouse ... arthritis with monoclonal antibodies to collagen J Immunol 1992, 148:2103-2108 Nandakumar KS, Holmdahl R: Efficient promotion of collagen antibody induced arthritis (CAIA) using four monoclonal antibodies ... effect of E coli lipopolysaccharide bypasses epitope specificity in the induction of arthritis with monoclonal antibodies to type II collagen Autoimmunity 1995, 22:137-147 Yoshino S, Ohsawa M:...
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Báo cáo y học: "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affini" doc
... C2B-16 to the mAbs (CII-6, CII-3, and C2B-14) to test the arthritogenicity We predicted that C2A-12 would exacerbate the arthritis because its ability to activate complement and bind to mouse ... arthritis with monoclonal antibodies to collagen J Immunol 1992, 148:2103-2108 Nandakumar KS, Holmdahl R: Efficient promotion of collagen antibody induced arthritis (CAIA) using four monoclonal antibodies ... effect of E coli lipopolysaccharide bypasses epitope specificity in the induction of arthritis with monoclonal antibodies to type II collagen Autoimmunity 1995, 22:137-147 Yoshino S, Ohsawa M:...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... T-cell receptor that can sequester SEB [11-14] The use of mouse monoclonal anti-SEB antibodies to study important epitope determinants essential for MHC/TCR binding has led others to explore the ... corresponding antibodies in CHO cells HuMAb ELISA reactivity to related toxins Related Staphylococcus enterotoxins (SEs), SEB, SEA, SED, SEC1, TSST-1 (Toxin Technology, Inc.), and tetanus toxoid (Cylex ... operator, or the cytokine analyzed, the EC50 of HuMAb154 always remained below ng/mL (Table 2) Crossreactivity of HuMAb-154 to other bacterial toxins To test whether HuMAb-154 crossreacted to other...
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