formation of gut like structures in vitro from mouse embryonic stem cells pdf
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2 /mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...
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Generation of hepatocyte like cells from mouse embryonic stem (ES) and bone marrow (BM) cells
... Generation of Hepatocyte -like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF OBSTETRICS ... cholangiocytes, Kuppfer cells, stellate cells, and a variety of endothelial cells including those lining the sinusoid (sinusoidal endothelial cells) (Blouin, Bolender et al 1977) The architecture of the liver ... murine ES cells They have two distinguishing features When cultured on feeder-cell layers of mouse embryonic fibroblasts, or in the presence of leukemia inhibitory factor (LIF), ES cells maintain...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... amount of glycoprotein in cells As shown in Fig 5E, although the total amount of protein in cells decreased to 87% after 48 h of incubation, the amount of glycoprotein decreased more The results of ... (PMI-null cells) [18] and wild-type mouse embryonic fibroblast cells (WT cells) were grown in DMEM containing 20% fetal bovine serum CDG-Ib patient cells were grown in a-MEM containing 10% fetal bovine ... with 200 lL of lipofectamine solution, dissolving lL of Lipofectamine 2000 in Opti-MEM, and transfected into subconfluent 293A cells in mL of Opti-MEM in six-well plates The 293A cells were then...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... http://www.jbiomedsci.com/content/18/1/46 Page of 10 Figure Confocal microscopy of a single colony of mouse embryonic stem cells A: top view of a single slice of scanning showing most eGFP signals are not overlapping with mitochondria ... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s Modified ... Conclusions In summary, a c-terminal truncated form of MsrA has been cloned from mouse embryonic stem cells due to the skipping of exon and subsequent frame shift in exon 6, generating a premature...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... UK) of the D3 clone Deletion of both alleles was confirmed by genotyping The ES cell lines were maintained in the undifferentiated state by culturing on gelatinized plates in KO-DMEM (Invitrogen, ... fibroblasts remained after 20-25 minutes plating in non-gelatinized plates Pre-plating of feeder-dependent ES cells in this way may result in a slight delay in the apparent time of replication ... replication in ES cells is altered by mutation of chromatin modifiers Satellite Satellite replication in ES cells is altered by mutation of chromatin modifiers (a) Summary of replication timing of repeat...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... Immunofluorescence (IF) staining………………………………… 53 3.7.2 Staining of tissue specimens……………………………………… 54 3.7.2.1 Processing of tissue specimens…………………………………… 54 3.7.2.2 Haematoxylin and eosin staining………………………………… ... in vitro model system to study cell fate determination, but may pose issues of contamination from the co-culture cell type affecting the yield and purity of the differentiated cells In this instance, ... differentiated in vitro, with extensive passaging during the process, the lower the risk of teratoma formation Indeed, lineage-restricted cell lines generated from hESCs demonstrated absence of teratoma formation...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... Genome-wide Mapping of Oct4-DNA Interactions in Mouse Embryonic Stem Cells 4.1 Introduction 74 4.2 Results 76 4.2.1 Optimisation of large-scale ChIP 76 4.2.2 Global mapping of Oct4 binding sites in mESCs ... Genome-wide Identification of Sox2-DNA Interactions in Mouse Embryonic Stem Cells 5.1 Introduction 95 5.2 Results 96 5.2.1 Optimisation of ChIP and global mapping of Sox2 binding sites in mESCs 96 5.2.2 ... 7.2 Function of genes associated with the binding of Oct4, Sox2, Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI xiii LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... We determined the H2O2-induced ROS generation in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed ... Min JJ, Park KS High glucose-induced inhibition of 2-deoxyglucose uptake is mediated by cAMP, protein kinase C, oxidative stress and mitogen-activated protein kinases in mouse embryonic stem cells ... activation of MAPKs signaling [29,58], thereby indicating that the MAPK signaling pathway is an important target of ROS The levels of intracellular ROS were increased as the result of the addition of...
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Dissecting transcriptional network in mouse embryonic stem cells
... type of stem cells, embryonic stem cells, from the mouse embryo Embryonic stem cell lines are derived from the inner cell mass (ICM) of the mouse blastocyst at embryonic day 3.5 (E3.5) These cells ... mixture of a large population of undifferentiated cells and differentiated cells of multiple lineages EC cells could be maintained indefinitely with mitotically inactivated embryonic fibroblast in vitro, ... expression of E-cadherin, suggesting that these cells are in a latent state of pluripotency Figure 1.1 Origin of stem cells during mammalian embryogenesis In this figure, the pluripotent cells of the...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonic stem cells (mESC) ... and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells (mESC) a Culturing Conditions ... Utilizing a stromal-derived inducing activity (SDIA) model of neurogenesis, we investigated the effects of the targeted knockdown of Oct-3/4 and Sox-2 by short interfering RNAs (siRNAs) in mouse embryonic...
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Báo cáo y học: " Dietary intake of fish, omega-3, omega-6 polyunsaturated fatty acids and vitamin D and the prevalence of psychotic-like symptoms in a cohort of 33 000 women from the general population" ppt
... pattern, indicating a reduced risk of psychotic -like symptoms among women with intermediate levels of intake After multivariate adjustment, the risk of high level psychotic -like symptoms for intake of ... Horrobins et al [43] This study surprisingly found that the effect of intermediate doses of EPA increased the levels of AA (an omega-6 PUFA) in the membrane of red cells, whereas higher doses of ... important in inhibiting the development of several diseases, including cancer, inflammatory and heart diseases [45,46] We find no support for this in our results, since, the effect of omega-3:omega-6...
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báo cáo khoa học: " New insight into the structures and formation of anthocyanic vacuolar inclusions in flower petals" potx
... Figure of in planta and in vitro isolated AVIs of the adaxial cells of the inner petal region of lisianthus flowers Micrographs Micrographs of in planta and in vitro isolated AVIs of the adaxial cells ... AVI being the final form The high levels of "unbound" anthocyanins observed in vacuoles containing the vesicle -like or rod -like AVI forms may indicate that the formation of AVIs in these cells ... micrographs of AVIs in the adaxial epidermal cells of the inner petal region of lisianthus flowers TEM micrographs of AVIs in the adaxial epidermal cells of the inner petal region of lisianthus...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... http://www.tbiomed.com/content/2/1/25 Figure Experimental embryonic stem cell colonies derived from a single blastomere Experimental embryonic stem cell colonies derived from a single blastomere of research in this area compared ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The...
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Formation of Aerobic Granular Sludge in a Continuous-Flow Reactor – Control Strategy for the Selection of Well-Settling Granular Sludge
... selection of well-settling sludge Then, we applied the strategy to the formation of aerobic granular sludge in a continuous experiment In addition, the effect of feeding pattern, i.e continuous ... stability of aerobic granules by selecting slow-growing nitrifying bacteria, J Biotechnol., 108(2), 161-169 McSwain B S., Irvine R L and Wilderer P A (2004a) The influence of- settling time on the formation ... granulation in a continuous-flow reactor Both surface loading and aeration rates affect the selection of well-settling sludge and the formation of aerobic granular sludge By setting and controlling adequate...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc
... in a single crossover event [11] From this library consisting of 50 strains with altered expression of the las operon, the enzyme activities of PFK, PK and LDH were determined and eight strains ... determined to 0.25 UÆmg)1 protein (B) Growth rates of selected strains (including flux control coefficients) Standard deviations, indicated by error bars, are based on measurement of three individual ... the second gene in the las operon (Fig 1) In order to obtain strains with increased PK activity an additional copy of the pyk gene was recombined into the TP901-1 attachment site using the site-specific...
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The formation of the plural noun in English and Vietnamese equivalents
... words which retain their original Greek or Latin forms make their plurals according to the rules of Greek and Latin Noun in -us (original Latin) the forein plural is -i as in: Eg: Singular form ... are often found preceded by a noun phrase that serves to make them countable A piece of furniture Many pieces of furniture 22 A bit of information Numerous bits of information A bottle of wine ... the formation of plural nouns in English and in Vietnamese 36 Chapter two:The formation of plural nouns in English and Vietnamese equivalents In English, the English number system comprises singular,...
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Báo cáo khoa học: FLIP and MAPK play crucial roles in the MLN51-mediated hyperproliferation of fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis pdf
... blocking of FLS apoptosis FLIP upregulated by MLN51 plays a crucial role in the anti-apoptosis of MH7A cells We examined whether FLIP was involved in the antiapoptosis of MH7A cells As shown in ... activated in MH7A cells (Fig 5A), as reported previously [21], ERK inhibitor (PD98059) does not affect MLN51 and FLIP induction in MH7A cells (Fig 5B), indicating that ERK is unlikely to be involved in ... regulation of the stress-activated protein kinases, extracellular signal-regulated kinase, c-JUN N-terminal kinase, and p38 mitogen-activated protein kinase, in synovial tissue and cells in rheumatoid...
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Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx
... times of inosine and hypoxantine, guanosine and guanine were 10.5 and 4.7 min, and 11.5 and 4.3 min, respectively The amount of purine base formed is determined by measuring the percentage of the ... phosphate binding preceding the nucleoside binding in the phosphorolytic direction The high kinetic stability of PfPNP is indicative of a compact and rigid structure that allows the protein to retain ... characterization of PfPNP and a comparative kinetic analysis of the two enzymes Initial velocity studies carried out with increasing concentrations of purine nucleosides in the presence of saturating concentration...
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Báo cáo khoa học: Engineering thermal stability of L-asparaginase by in vitro directed evolution ppt
... activity Observed rates of inactivation (kin) Engineering thermal stability of L-asparaginase were deduced from plots of log (% remaining activity) versus time Guanidinium chloride induced subunit dissociation ... enzyme In principle, the entropy of inactivation consists of two parts: the increased configurational entropy due to partial unfolding of the protein in the transition state and the decrease in entropy ... This incompatibility often relates to the stability of the enzymes under body conditions In the case of engineering proteins for thermostability, researchers are in the enviable position of being...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... tethering, thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the ... purification of recombinant mPC1 ⁄ and hfurin Recombinant murine PC1 ⁄ was produced using the baculovirus expression system in Sf9 insect cells [7] or through intracoelemic injection in insect larvae ... is explained by the role of C-terminal determinants in allowing proper secretion, correct folding or targeting Nevertheless, a cooperative inhibitory interaction between N- and C-terminal propeptides...
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