... Ge-PAI B Junctions Junction Stability of Ge-PAI B Junctions with Various FLA Schemes 6.5 Dopant Activation of Ge-PAI Junctions with Various FLA Schemes 6.6 SIMS Profiling of Ge-PAI Junctions ... Professor Andrew Wee of Physics Department, National University of Singapore for the support of Secondary Ion Mass Spectrometry Part of this work also involved collaboration with University of ... Diffusion Profiles (b) Nitrogen Diffusion Profiles 117 121 125 125 130 Table of Contents (c) EOR Defects 4.4.5 A Summary of the Effect of Nitrogen Distribution on Boron Diffusion 4.5 The Impact of N...
... displacement of approximately 10~12 % of the lattice atoms Formationof an amorphous layer progresses gradually, as damage to the crystal lattice is accumulated with each implanted ion The number of ions ... melting of the damaged region and subsequent liquid-phase re- crystallization of the melt The process is characterized by extremely short times of phase transformation, with typical laser pulses of ... by applying a high dc potential The impact of these primary ions causes the surface layer of atoms of the sample to be sputtered off A small fraction of these atoms is ejected as positive (or...
... the washout of sludge from the reactor, and therefore, we investigated the effect of surface loading rate and aeration rate on the selection of well-settling sludge and the formationof aerobic ... view Fig - Schematic diagram of the AUFB reactor Reactor Setup and Operation for the Formationof Aerobic Granular Sludge Two AUFB reactors were used for the formationof aerobic granular sludge ... strategy for the selection of well-settling sludge Then, we applied the strategy to the formationof aerobic granular sludge in a continuous experiment In addition, the effect of feeding pattern, i.e...
... the assembly of the bc1 complex is also unknown Furthermore, as in the case of the biogenesis of other multi-subunit complexes of the mitochondrial respiratory chain, the assistance of specific ... insertion of ISP into an immature bc1 complex and that the association of the Qcr9p subunit with the bc1 complex precedes the binding of ISP In fact, the direct absence of ISP (DISP), or the block of ... incorporation of ISP, even if further functions cannot be excluded A possible role of this chaperone in the stabilization of the core structure of the bc1 complex can be excluded on the basis of the...
... attributed to formationof the Fe(III)–M80 axial bond, providing strong indication that the slow phase is coupled to formationof the native axial coordination The absorption spectra of the K13N ... magnification of original spectra (D) Absorption spectra of the aciddenatured K13N mutant (spectrum a), of the K13N mutant after ms of mixing with 40 mM sulfate (spectrum b), and after s of mixing ... used of analytical grade from purifrom were Construction of horse cytochrome c expression system A version of the horse cytochrome c synthetic gene was designed on the basis of the sequence of...
... the extent of fibril formation overall for both the WT and V30M TTR proteins The extent of fibril formation can be calculated as the percentage of the turbidity (absorbance at 330 nm) of the oxidized ... effects caused a delay in the onset of amyloid fibril formation Alternatively, the inhibition of fibril formation may purely be the result of an increase in solubility of oxidized proteins [6,41] Limited ... method of investigating regions of proteins that are critical in the onset of amyloid formation This study reveals that domains neighboring methionine residues are critical in the formationof fibril...
... aggregate formationof an alkaline phosphatase frame-shift mutant Hypophosphatasia is an inborn error of metabolism characterized by defective mineralization of hard tissues and reduced levels of tissue ... assembly of TNSALP in the endoplasmic reticulum (ER), resulting in the loss of functional TNSALP from the cell surface [10–14] Deletion of T at position 1559 of the cDNA [TNSALP (1559delT)] of the ... concerted action of nucleoside triphosphate pyrophosphohydolase and TNSALP regulates local concentration of inorganic pyrophosphate at the site of mineralization, which, as a poison of hydroxyapatite...
... well with the individual determination of flux control coefficients by PFK, PK and LDH The role of the las operon is discussed on the basis of the distribution of flux control for PFK, PK and LDH Results ... unclear but may reflect a combination of a hierarchical up-regulation [16] of the las operon at low PK activity and a polar effect of the pyk deletion on expression of ldh An important question is ... were related to the A280 of the extract, for the purpose of determining relative activities The specific activities of PFK and PK and LDH in MG1363 were determined as UÆmg)1 of protein, where a unit...
... Letter of the bishop of Vienne to the Roman senate upon this Council, 139 The cause of the Bishop of Rome is not that of one bishop, but of the Episcopate itself, 140 Words of Ennodius, bishop of ... see of Constantinople any rank beyond that of an ordinary bishop, and omits the Council of 381, 116 CHAPTER II Death of Pope Gelasius and character of his pontificate, 118 His own description of ... http://www.archive.org/details/theholysee06alliuoft THE HOLY SEE AND THE WANDERING OF THE NATIONS FROM ST LEO I TO ST GREGORY I by THOMAS W ALLIES, K.C.S.G Author of the "Formation Of Christendom"; "Church and State As Seen in the Formation...
... Ó FEBS 2002 Formationof the tripeptidyl-peptidase II complex (Eur J Biochem 269) 1439 of the oligomeric complex could be a way of regulating the enzymatic activity In ... human 293-cells The main objective of the present work was to find an explanation for this phenomenon It is demonstrated that the formationof the active complex is profoundly influenced by a single ... dithiothreitol, at a flow rate of mLÆh)1 Fractions of mL were collected The void-volume (Vo) and total volume (Vt) of the column were determined from the elution positions of Blue dextran (AP Biotech,...
... prediction of amyloidogenic regions in a protein sequence (or rather, the ability of a peptide to be amyloidogenic): the scale of the packing density, and two scales of the probability offormationof ... correctly predicts 80% of amyloidogenic peptides (115 of 144 peptides) and 72% of non-amyloidogenic ones (189 of 263 peptides), with a cut-off value of 0.062, from a database of 407 amyloidogenic ... cut-off (have numbers of expected contacts higher than the cut-off) and the size of the region is greater than or equal to the size of the sliding window used Optimum values for the cut-off (threshold)...
... influences fibril formationof Ab(1-40) and Ab(1-42) LPC modulates Ab fibril formation Results Effects of LPC on Ab(1-42) fibril formation To investigate the effects of LPC on Ab(1-42) fibril formation, ... maximum of Tyr fluorescence is approximately 304 nm [23] Fibril formationof Ab peptides in the absence or presence of LPC did not produce any shift of the Tyr fluorescence maximum Incubation of Ab(1-40) ... fibril formation reaction was terminated by quickly freezing the samples Assessment of Ab fibril formation on the basis of ThT fluorescence The presence of b-sheet structures and the kinetics of fibril...
... involvement of PFD in the formationof toxic Ab oligomers in the cytosol Results Fibrillation of Ab peptide in the presence of PFD In an effort to investigate the effects of PFD on fibrillation of Ab(1–42) ... addition of lm Ab fibrils formed in the absence of PFD By contrast, addition of only 0.05 lm soluble Ab oligomers formed in the presence of PFD markedly induced PC12 cell death The observed level of ... Fig Dot-blot assay of soluble Ab oligomers formed in the presence of PFD Samples of Ab oligomers formed in the presence of PFD (Ab ⁄ PFD), Ab fibrils formed in the absence of PFD (Ab fibril), A11-positive...
... 2-arylpropanoyl-CoA was produced by way of an acyladenylate intermediate Kinetic analysis of LUC-H catalyzed thioester formation The kinetic study of the formationof ketoprofenylCoA catalyzed by LUC-H ... antiinflammatory drugs ) In vitro studies of ibuprofen and flurbiprofen Biochem Pharmacol 38, 4389–4395 Hall SD & Xiaotao Q (1994) The role of coenzyme A in the biotransformation of 2-arylpropionic acids Chem ... questions In the case of recombinant rat LACS1, the substrate specificity was not investigated in detail and only three kinds of substrate, ibuprofen, fenoprofen, and flurbiprofen, were examined...
... model of inhibition of CrkII complex formation, regulated by the intramolecular binding of SH2 to phosphorylated Tyr221, there is an established sequence of mechanisms regulating CrkII complex formation ... electrophoretic mobility and the formationof CrkII protein complexes Concerning the regulation of CrkII complex formation, it has been proposed that the SH2 domain of CrkII intramolecularly binds ... activator of the tyrosine phosphorylation of different proteins such as p130Cas [7] and paxillin [6], creating potential binding sites for the SH2 domain of CrkII, necessary for the formationof protein...
... amounts of cyt b5 in presence of endogenous levels of P450red In presence of these low levels of P450red, cyt b5 shows a slight stimulatory effect on DHEA formation However, we observe a more profound ... culture, we compared the formationof Ó FEBS 2003 Biosynthesis of 5,16-androstadien-3b-ol by human P450c17 (Eur J Biochem 270) 1353 Fig Role of cyt b5 in the formationof androstadienol from pregnenolone ... of only endogenous P450red levels, DHEA formation from preg is below 10% Increasing P450red up to 0.25 lg causes a drastic increase of DHEA formation, reaching levels up to 50% of preg transformation...
... presence of HNE ( 0.80 ± 0.18-fold over control cells) Fig Formationof tau aggregates in neuroblastoma cells SH-SY5Y neuroblastoma cells were incubated in the absence (A), or presence of 0.25 ... carrying some of the mutations present in a tauopathy, frontotemporal dementia linked to chromosome 17 (FTDP-17), results in the expression of the mutated protein and in the formationof aberrant ... involved in aggregation of the protein but not in the formationof filaments Cell Mol Biol 44, 1111–1116 21 Schweers, O., Mandelkow, E.M., Biernat, J & Mandelkow, E (1995) Oxidation of cysteine-322 in...
... sequenced The full-length cDNA of Pun1 had a length of 1185 bp coding for a protein of 395 amino acids with a calculated molecular mass of 45.8 kDa The amino acid sequence of this putative fatty acid ... r.p.m) to densities of A600 ¼ 2–3 Twenty millilitres of cell cultures were harvested by centrifugation and lyophilized Lipid analysis For analysis of punicic acid content of transformed yeast ... equal volume of PCI (phenol/chloroform/ isoamyl alcohol, 20 : 20 : 1, v/v/v) After centrifugation at 3000 g for 10 the hydrophilic phase was reextracted with an equal volume of chloroform and the...