formation cumulus cells for somatic cell nuclear transfer scnt and mouse fetuses for primary embryonic fibroblast pefs preparation
Báo cáo khoa học: "Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal" pdf
... and then cultured for an additional 18 h Preparation of donor cells Four types of somatic cells were used as donor cells: 1) denuded cumulus cells (DCCs); 2) cultured cumulus cells (CCCs); 3) ... The cumulus cells used in this study were obtained from IVM oocytes In addition, cumulus cells and follicular cells were cultured for 18 and 40 h, respectively, before they were used as donor cells, ... blastocyst formation (1 ± 0%) was obtained from autolo- gous SCNT using oocytes and cumulus cells from the same pig than from SCNT using autologous follicular cells and heterologous skin fibroblasts...
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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos" pps
... elirets ni yrotarobal eht ot detropsnart dna riottaba lacol a ta detcelloc erew stlig latrebup-erp morf seiravo enicroP MVI dna noitcelloc etycoO ]92[ etatcal Mm 0.5 dna etavuryp Mm 5.0 htiw esoculg ... gnosnuE ,gnoS gnuoyliK 68 .72-11 ,411 ,8891 seR taidaR AND tcetorp ot ytiliba sti dna loiht a no egrahc ten eht neewteb pihsnoitaler :sloiht yb AND fo noitcetorpoidaR FJ draW ,CR yehaF ,G kcinoG ... tnarG noitadnuoF hcraeseR aeroK a yb detroppus saw krow sihT seiravo gip fo noitatropsnart dna noitcelloc rof eeL nuegnuoY sM dna ,eeL gnuoynI sM ,nowK nuyhoB rM knaht srohtua ehT stnemgdelwonkcA...
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Báo cáo khoa học: "Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes" pot
... hormone-free IVM medium before being cultured again in hormone-free IVM medium for an additional 20 h for SCNT and 22 h for parthenogenetic activation (PA) Preparation of donor cells Two sei whales ... previously for pig SCNT [26], and have been shown to be acceptable for the membrane fusion of whale somatic cells with pig oocytes In iSCNT embryo culture, it is common to use a medium specific for ... saline (pH 6.8) for 10 Sei whale fetal fibroblasts used as donor cells in this study had normal diploid (2n = 44) chromosomes (Fig 1) Nuclear transfer After 42 h of IVM, cumulus cell- free oocytes...
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Báo cáo khoa học: " Resistance to penicillin of Staphylococcus aureus isolates from cows with high somatic cell counts in organic and conventional dairy herds in Denmark" potx
... according to standard procedures [17] Data on milk production and SCC from monthly test days and information on breed, age, and calving number were available from the Danish Cattle Database for at least ... also provide the necessary information for choosing the most effective preventive measures for controlling udder infections in general Abbreviations SCC: Somatic cell count Competing interests ... Laboratory for collecting and analyzing of milk samples This work was supported by the Danish Dairy Board and by the Danish Ministry for Food, Agriculture and Fisheries through the Research Centre for...
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Báo cáo y học: "Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling" ppt
... Myeloid cells Figure Clustering of mouse IKDCs and human CD16 cells Clustering of mouse IKDCs and human CD16 cells Hierarchical clustering with complete linkage was performed on the indicated cell ... FR: Genomic profiles for human peripheral blood T cells, B cells, natural killer cells, monocytes, and polymorphonuclear cells: comparisons to ischemic stroke, migraine, and Tourette syndrome ... functions for the leukocyte subsets studied, as indicated in bold in Table These include, for example, Cd19 and Pax5 for B cells [35], Cd3e-g and Lat for T cells [36], as well as Ncr1 [37] and Tbx21...
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Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score" ppt
... SCS-BTA18-Q and SCS-BTA18-q cells and respective control cells; the functional category, p-values and the number of genes are shown for E coli inoculated SCS-BTA18-Q and SCS-BTA18-q cells and S aureus ... between inoculated cells and control cells at time points for each pathogen challenge and each of the inherited SCS-BTA18QTL alleles; A E coli inoculated cells; B S aureus inoculated cells; C E coli ... was performed at time point zero and control cells and inoculated cells derived from the same cell culture, no differences regarding gene expression between the inoculated and control cells were...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... cumulus cells For example, prostagrandin E2 (PGE2) that produced from granulosa cells and cumulus cells by prostagradin synthase (PTGS2) was required for induction of Has2 and Tnfaip6 gene, cumulus ... granulosa cells and cumulus cells by LH stimuli acted on cumulus cells to induce http://www.ovarianresearch.com/content/2/1/20 cumulus expansion, meiotic maturation of oocytes and ovulation in mouse ... stimuli in granulusa cells, and EGF receptor (EGFR) was localized on cumulus cells [9-11] When mouse COCs were cultured with AREG, Has2, Tnfaip6 and Ptgs2 were expressed in cumulus cells TACE/ ADAM17,...
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Báo cáo khoa hoc:" Genetic analysis for mastitis resistance and milk somatic cell score in French Lacaune dairy sheep" ppsx
... Repeatability model for all test-days from DIM 25 to 174 Standards errors between 0.011 and 0.034 Standards errors between 0.029 and 0.119 for genetic correlations and between 0.012 and 0.075 for environmental ... Mazette R., Some microbiological causes for the increase in somatic cells in sheep milk, in: Proceedings of the International symposium on somatic cells and milk of small ruminants, Bella, 25–27 ... increased culling rate, and increased cost and labour for detection and veterinary treatment Furthermore, a high somatic cell count (SCC) in milk may reduce the price of milk for the farmer by more...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
... tumor cells In particular, AK1C1, and AK1C3 are reported in human non-small cell lung carcinoma (A549) cells [21], and a high expression of AL1A1 in lung cancer cell lines, especially in AC cell ... retinoic acid and the precursor of the storage form vitamin A, respectively CD44 is one of cell- surface glycoproteins which relates to cell- cell interactions including adhesion and migration, and thus ... LCC) and the number of male accounts for over 80% for all groups The majority of patients remained at stages from IA to IIB and accordingly had the extent of the primary tumor (T1 and T2) and...
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Báo cáo y học: "Genome-wide identification of novel expression signatures reveal distinct patterns and prevalence of binding motifs for p53, nuclear factor-κB and other signal transcription factors in head and neck squamous cell carcinoma" docx
... were 63% for NF-κB, 60% for AP-1, 100% for STAT3, and 76% for EGR1 Similarly, in cluster B, the prevalence of binding sites falling in orthologous promoters were 65% for NF-κB, 67% for p53 and AP-1, ... population of UM-SCC 11B cells with a heterogeneous expression pattern for nuclear p53 protein (Figure 1b) The findings regarding p53 mutation in UM-SCC 1, 5, 6, 11B, and 46 cells are consistent ... cells Figure p53 genotype and protein expression in UM-SCC cell lines p53 genotype and protein expression in UM-SCC cell lines (a) The p53 genotype of ten University of Michigan series head and...
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Báo cáo sinh học: "Genetic parameters for somatic cell score according to udder infection status in Valle del Belice dairy sheep and impact of imperfect diagnosis of infection" pptx
... has been shown that cells in the milk from a healthy udder are mainly mammary gland epithelium and drain canal cells; whereas polymorphonuclear leukocytes (PMN) are the major cell population during ... geometric mean SCC was 403 (× 103 cells/ mL) for the whole data, 253 for the bacteria negative, and 1,082 for the bacteria positive Although ranges of SCC for uninfected and infected animals were similar, ... Regulations for milk recording in sheep Institut de l’élevage, Department Génétique ei Contrôle des Performances Paris 2003 13 Ali AKA, Shook GE: An optimum transformation for somatic cell concentration...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... hPS cells (hES and iPS cells) into long term proliferating pluripotent stem cell- derived neuroepithelial stem (lt-NES) cells was performed as described previously (Koch et al., 2009) HPS cells ... 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells share ... germ layers of the embryo The cells of the inner cell mass, known as embryonic stem cells, can be extracted and cultured in vitro, and are able to generate any cell type of the mature organism...
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Tài liệu HEALTH EDUCATION THROUGH INFORMATION AND COMMUNICATION TECHNOLOGIES FOR K-8 STUDENTS: CELL BIOLOGY, MICROBIOLOGY, IMMUNOLOGY AND MICROSCOPY ppt
... organelles, and structures and functions of the bacteria covering issues from different aspects of cells including cell biology, microbiology, immunology and microscopy In terms of cell biology, cell ... Journal of Theoretical and Applied Information Technology © 2007 JATIT All rights reserved www.jatit.org (1999) E -CELL: Software environment for whole cell simulation Bioinformatics, 15,72-84 15 ... different aspects and subspecialties of health education The current study is aimed at providing accurate and concrete information to K-8 students on the correct functions of our cellular and viral system...
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Guidelines for Human Embryonic Stem Cell Research potx
... become cells with special functions, such as cardiomyocytes (the beating cells of the heart), liver cells, nerve cell precursors, endothelial cells, hematopoietic cells, and insulin-secreting cells ... of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... induce mES cells to differentiate into specific cell types in vitro, including hematopoietic stem cells, beating cardiac muscle cells, neuronal progenitors, endothelial cells, and bone cells In...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... Effect of ATP and ADP on cell cycle progression of LXF-289 cells (A) Accumulation of LXF-289 cells in the S phase LXF-289 cells were incubated with ATP or ADP (10–100 lM) for 24 h, and the distribution ... 104 cells per well; 500 lL per well) At 24 h after seeding, cells were treated with 100 lm ATP or ADP for or days Antagonists were added to the cells h before the addition of the nucleotides Cells ... the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cell cycle after treatment with 20 lM or 100 lM ATP for 24 h The gating used for the quantification of the cells in the G0 ⁄ G1 (a), S (b) and...
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Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf
... template and alignment [18] FEBS Journal 272 (2005) 3052–3059 ª 2005 FEBS Expression of mSMOa, mSMOl and mSMOlD isoforms in E coli cells E coli BL21 DE3 (Novagen) cells transformed with the pmSMOa and ... DNA marker (MBI Fermentas); NT, untransfected cells; P, cells transfected with pcDNA3-V5-TAG; Ta, l and lD, cells transfected with pcDNA3 ⁄ mSMOa, l and lD, ⁄ V5-TAG plasmids; C, no-template control ... Expression and purification of mSMOlD protein in Escherichia coli cells The recombinant cDNA construct, pmSMOlD-HT, and the controls pmSMOa-HT and pmSMOl-HT, were used to transform E coli BL21 DE3 cells...
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CULTURE OF CELLS FOR TISSUE ENGINEERING potx
... and R Ian Freshney, Editors CULTURE OF IMMORTALIZED CELLS R Ian Freshney and Mary G Freshney, Editors DNA TRANSFER TO CULTURED CELLS Katya Ravid and R Ian Freshney, Editors CULTURE OF CELLS FOR ... proteins, for example, cytokeratins for epithelial cells, vimentin for mesodermal cells such as fibroblasts, endothelium, and myoblasts, desmin for myocytes, neurofilament protein for neuronal and some ... concentration For example, if cells are frozen at × 106 cells in ml of freezing medium with 10% DMSO and then thawed and diluted 1:20, the cell concentration will still be 2.5 × 105 cells/ ml at...
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Báo cáo khoa học: Acetylcholinesterase in cell adhesion, neurite growth and network formation pot
... of receptors for extracellular matrix components, thereby providing cells with an avenue for the transfer of information from the extracellular space to the cytoskeleton and intracellular signaling ... the central and peripheral nervous systems, different isoforms are found in haematopoietic cells, osteoblasts, vascular endothelial cells and leukocytes, and are induced in various cell lines ... neurite growth and neural network formation in vivo and in vitro (Left) Formation of synaptic sublayers is disturbed in the inner plexiform layer of a P19 retina of the AChE) ⁄ ) knockout mouse (lower),...
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Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx
... were: DmPCNA2 – 94 °C for min, 94 °C for 45 s, 55 °C for 45 s, 72 °C for min, 24 cycles, extension at 72 °C; DmPCNA1 ) 94 °C for min, 94 °C for 45 s, 55 °C for 45 s, 72 °C for min, 21 cycles, extension ... proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon Mol Cell Biol 17, 6367–6378 Maga G & Hubscher U (2003) Proliferating cell nuclear ... high-salt wash and the core nuclear matrix from S2 cells As controls for the fractionation procedure, we used western blotting with antibodies against b-tubulin (a nonchromatin-bound protein) and histone...
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Đề tài " Formation of singularities for a transport equation with nonlocal velocity " potx
... where Γ1 and Γ2 are the paths in the complex plane represented in Figures and respectively Standard pole integration for I1 and the fact that Γ2 \{c1 ,c2 } = − {c1 ,c2 } in I2 (cf Lemmas 2.2 and 2.3 ... on θ0 and ν, such that for t ≥ 0: ≤C, (3.16) 1) Λ2 θ (3.17) 2) Λθ L2 (t) ≤ C(1 + t) , (3.18) 3) ∆θ L2 (t) ≤ CeCt L2 (t) Proof Integration by parts and the formula for the Hilbert transform 2H(f ... nonlocal velocity and show the formation of singularities in finite time for a generic family of initial data By adding a diffusion term the finite time singularity is prevented and the solutions...
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