formation activation and aggregation of platelets b
Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx
... Shiraki et al (Eur J Biochem 271) Table Rates of < /b> thermal inactivation and < /b> aggregation < /b> in the presence of < /b> additives Thermal inactivation and < /b> aggregation < /b> in the presence or absence of < /b> 100 mM additive ... rates of < /b> inactivation and < /b> aggregation < /b> of < /b> lysozyme in the presence of < /b> ArgEE were similar to those in spermine, which is a favourable additive to prevent thermal inactivation and < /b> aggregation < /b> of < /b> lysozyme ... different conditions are shown in Table The rates of < /b> inactivation and < /b> aggregation < /b> depend on the protein Fig Thermal inactivation and < /b> aggregation < /b> of < /b> lysozyme in the presence of < /b> additives The samples containing...
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Tài liệu Báo cáo khoa học: Prevention of thermal inactivation and aggregation of lysozyme by polyamines docx
... stable mutants with amino acid pairs substituted for the disulfide bond between residues 14 and < /b> 38 of < /b> bovine pancreatic trypsin inhibitor (BPTI) J Biol Chem 277, 51043–51048 23 Volkin, D .B & Klibanov, ... inactivation of < /b> proteins is caused by both noncovalent and < /b> covalent modifications, including disulfide exchanges [23,24,28], b- elimination of < /b> disulfide bonds [24,28], and < /b> deaminations of < /b> Gln and/< /b> or ... has been obtained that heat inactivation of < /b> trypsin is effectively inhibited by polyamines 21 Second, can polyamines prevent the formation < /b> of < /b> other types of < /b> aggregates, such as fibril formation...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... present in the genomes of < /b> the honeybee Apis mellifera and < /b> the red flour beetle Tribolium castaneum [21,22] A probable ortholog of < /b> Spatzle-1 has been studied in the silkworm, ¨ Bombyx mori [23] A aegypti ... these bands by MS ⁄ MS and < /b> mascot software identified them as attacin-1, lysozyme, and < /b> cecropinA ⁄ B (Table S3) Immunoblot analysis with antibody to M sexta lysozyme confirmed the elevated level of < /b> ... proHP8Xa could be activated by factor Xa, 50 ng of < /b> purified recombinant proHP8Xa was incubated with 100 ng of < /b> bovine factor Xa (New England BioLabs, Ipswich, MA, USA) in the reaction buffer (20 mm...
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Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx
... controls The subcellular distribution of < /b> PLD and < /b> the consequences of < /b> priming and < /b> activation < /b> were determined using immunoprecipitation and < /b> Western blot analysis of < /b> neutrophil cytosol and < /b> membrane fractions ... concentration of < /b> 0.5% (v/v) Cytosolic and < /b> membrane fractions were immunoprecipitated and < /b> Western blotted as above Antibody-bound proteins were detected by ECL The specificity of < /b> the pan-PLD1/2 antibody ... kDa and < /b> 350– 400 kDa) [9,10] Using RT-PCR, we have been able to show the presence of < /b> both PLD1 and < /b> PLD2 isoforms in human peripheral blood neutrophils, with PLD1 being the more abundant isoform...
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Adsorption and aggregation of surfactants in solution
... interfacial area A soap bubble is stabilized by monolayers on both inside and < /b> outside surfaces of < /b> the bubble (Fig 4) The glycerol and < /b> enzyme can be added into the aqueous phase before producing the ... time of < /b> textiles, bubble volume, emulsion droplet size, and < /b> solubilization of < /b> benzene in micellar solution was found [18] 10 Shah and < /b> Moudgil FIG Two relaxation times of < /b> micelles, and < /b> 2, and < /b> ... edited by I B Ivanov Microemulsions and < /b> Related Systems: Formulation, Solvency, and < /b> Physical Properties, edited by Maurice Bourrel and < /b> Robert S Schechter Crystallization and < /b> Polymorphism of < /b> Fats and...
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Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
... discriminating between the role of < /b> Ca2+ in activation < /b> and < /b> catalysis J Biol Chem 270, 18563–18569 33 Bu, C.H & Pourmotabbed, T (1996) Mechanism of < /b> Ca2+dependent activity of < /b> human neutrophil gelatinase B J Biol ... of < /b> THP-1 cells and < /b> the 72 kDa form of < /b> proMMP-2 in serum-free culture medium of < /b> human skin fibroblasts The arrow shows the border between the stacking and < /b> the separating gel Fig Urea inhibits both ... function of < /b> Ca2+ in the action of < /b> mammalian collagenases Arch Biochem Biophys 173, 355–361 32 Bu, C.H & Pourmotabbed, T (1995) Mechanism of < /b> activation < /b> of < /b> human neutrophil gelatinase B: discriminating...
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formation, microstructures and crystallization of anodic titanium oxide
... thickness of < /b> about 40 mm has been produced (Fig 2a) A top view on the opened ends of < /b> the nanotubes (Fig 1b) and < /b> a bottom view on the closed ends of < /b> the nanotubes (top part of < /b> Fig 2b) show that ... nanotubes are almost hexagonally ordered The morphology of < /b> the bottom of < /b> nanotubes seems to be hemispherical However, some distortions and < /b> non-uniform wall thicknesses of < /b> the nanotubes can be seen ... coefficients (Fig 3b) , confirming that these two layers have different compositions and < /b> an obvious boundary Infrared spectrum of < /b> the as-prepared ATO shows a peak at $1630 cmÀ1 and < /b> a broad band between 3000...
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Báo cáo khoa học: Thermal unfolding and aggregation of actin Stabilization and destabilization of actin filaments doc
... controlled not only by nucleotide binding and < /b> hydrolysis, but also by the action of < /b> a number of < /b> actin-binding proteins that can nucleate, cleave, cross-link, bundle, stabilize or destabilize the filaments ... in subdomain 4) from the body of < /b> the subunit to form a contact with subunits on the opposite strand of < /b> a double-stranded spiral polymer [15] According to the Holmes model of < /b> F-actin [16], subdomains ... between several sites in subdomain and < /b> sites on subdomains and < /b> 2, as well as contact between subdomains and < /b> [13], and < /b> the DNase I-binding loop (D-loop) at the top of < /b> subdomain is proposed to interact...
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Báo cáo khoa học: Effects of sphingomyelin, cholesterol and zinc ions on the binding, insertion and aggregation of the amyloid Ab1)40 peptide in solid-supported lipid bilayers ppt
... lipid layer before and < /b> after binding of < /b> peptide and < /b> subsequent aggregation,< /b> and < /b> of < /b> the layer composed of < /b> peptide (or a portion of < /b> the peptide) that extends beyond the lipid surface Both p- and < /b> s-polarized ... (solid line), and < /b> the binding affinity values and < /b> the magnitude of < /b> the spectral shift are given in Table Interaction of < /b> Ab with binary lipid bilayers As shown by the data in Fig 3, and < /b> by the KD values ... inhibit the fibril formation < /b> J Biol Chem 277, 6273–6279 Bitan G, Kirkitadze MD, Lomakin A, Vollers SS, Benedek GB & Teplow DB (2003) Amyloid beta -protein (Abeta) assembly: Abeta 40 and < /b> Abeta 42 oligomerize...
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báo cáo khoa học: " Transcriptional activation and localization of expression of Brassica juncea putative metal transport protein BjMTP1 Balasubramaniam Muthukumar, Bakhtiyor Yakubov and " ppsx
... site of < /b> BjMTP1 is sufficient to drive ABRE (-742) bZIP (-728) G box (-729) W box (-713) EIRE box (-714) SEBF motif (-640) GCC box (-606) MYB core (-293) SEBF motif (-267) G box coupler (-240) MYB ... such as the CCAAT boxes, ABRE (Abscisic Acid Response Element), bZIP, G box, and < /b> several pathogen responsive elements such as W box, EIRE, SEBF motif, GCC box, G box coupler and < /b> MYB core elements ... transcriptional activation < /b> of < /b> BjMTP1 by its Figure 983 bp promoter Metal regulated transcriptional activation < /b> of < /b> BjMTP1 by its 983 bp promoter Five-day-old dark grown seedlings, transformed with p (1.0) BjMTP1::GUSPlus,...
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Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf
... (Figure 1) Inhibition of < /b> L-TGF-β1 activation < /b> by Lv-shCD36 in BALF The activation < /b> of < /b> L-TGF-β1 was determined by detecting the quantity of < /b> TGF-β1 in BALF using the CCL-64 growth inhibition assay ... could inhibit activation < /b> of < /b> L-TGF-β1 by decreasing expression of < /b> CD36 on the membrane of < /b> AMs to further reduce the combination of < /b> CD36 with TSP-1/L-TGF-β1 Table 1: Silicatic nodule grade of < /b> the ... Rifkin DB, Murphy-Ullrich JE: Latent Transforming Growth Factor-{beta}-binding Protein-4 Regulates Transforming Growth Factor-{beta}1 Bioavailability for Activation < /b> by Fibrogenic Lung Fibroblasts...
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Báo cáo y học: " Legionella pneumophila infection induces programmed cell death, caspase activation, and release of high-mobility group box 1 protein in A549 alveolar epithelial cells: inhibition by methyl prednisolone" pot
... MOI of < /b> 100 After hours incubation, extracellular bacteria were removed by washing, and < /b> the infected cells were cultured further The number of < /b> viable bacteria in each well was determined by the ... DNA fragmentation of < /b> AA100jm-infected A549 cells is inhibited by methyl prednisolone, and < /b> that this inhibition of < /b> cell injury is accompanied by the degradation of < /b> caspase 3, 8, and < /b> We therefore ... research papers will be: available free of < /b> charge to the entire biomedical community peer reviewed and < /b> published immediately upon acceptance cited in PubMed and < /b> archived on PubMed Central yours...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
... and < /b> were recognized by the epitope-specific CTLs Establishment of < /b> MOLT-4 cells stably expressing SCTs of < /b> RT1.Al To examine the effects of < /b> rat SCTs expressed on human cells and < /b> the influence of < /b> ... dramatically enhanced by FPM1.BP cells (Figure 3D) These results suggested that the growth inhibition by SCTs with Tax resulted from both an enhanced level of < /b> AICD and < /b> a reduced activation < /b> of < /b> proliferation ... animal models Rats have been used for a number of < /b> studies on HTLV-I infection, because they are susceptible to the virus and < /b> because the virus-transformed T cell lines can be established in vitro [33,34]...
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the interactions between inflammasome activation and induction of autophagy following pseudomonas aeruginosa infection
... ATPIF1 ATPase inhibitory factor BIR Baculoviral inhibitory repeat like domain BM Bone marrow BMDM Bone marrow derived macrophages BrdU Bromodeoxyuridine BSA Bovine serum albumin CARD Caspase ... mаy or mаy not be plаsmid-mediаted but can be explained by the permeability barrier afforded by its Gram-negative outer membrane and < /b> its tendency to form a biofilm layer on colonized surfaces, ... relieves this inhibition and < /b> is thus a potent inducer of < /b> autophagy The subsequent steps of < /b> the process can be broken down into selection of < /b> cargo and < /b> packaging, nucleation of < /b> the autophagocytic...
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Different functions of notch activation on formation and maintenance of rhombomere boundaries
... images of < /b> the surfaces: (a) DCHB and < /b> (b) DCHB-PAAc beads Figure 6.2 FTIR spectra of < /b> (a) DCHB and < /b> (b) DCHB-PAAc beads Figure 6.3 XPS C 1s core-level spectra of < /b> (a) DCHB and < /b> (b) DCHB-PAAc beads ... CHBs, (b) FCHBs, (c) EFCHBs, and < /b> (d) NRCHBs Figure 4.3 XPS O 1s spectra for (a) CHBs, (b) FCHBs, (c) EFCHBs and < /b> (d) NRCHBs Figure 4.4 SEM images showing the surface morphologies of < /b> (a) CHBs, (b) ... chitosan-cellulose beads Table 3.4 Mechanical test results of < /b> the different types of < /b> chitosan beads Table 5.1 Assignments of < /b> infrared absorption bands Table 5.2 Assignments of < /b> C 1s spectra bands based on...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... constant of < /b> the JI phase compared to the amplitudes and < /b> rate constants of < /b> the two other phases The above should be governed by the balance between the rate of < /b> reduction and < /b> oxidation of < /b> the acceptor ... membranes can be modeled by the superposition of < /b> the exponential rise to analyze quantitatively the contribution of < /b> each phase The amplitude and < /b> rate constant of < /b> each of < /b> the three phases can be ... chlorophyll-a and < /b> chlorophyll -b extracted with different solvents ) verication of < /b> the concentration of < /b> chlorophyll standards by atomic-absorption spectroscopy Biochim Biophys Acta 975, 384394 FEBS Journal...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... formation < /b> to the conformational stability of < /b> active onconase In order to evaluate the contribution of < /b> the (Met1) hydrolysis and < /b> pyroglutamyl formation < /b> to the global conformational stability of < /b> ... almost linear and < /b> can be obtained by calculating the slope of < /b> the curves of < /b> Pyr-ONC (M23L) vs time From the rates of < /b> cyclization shown in Table 1, it can be inferred that the formation < /b> of < /b> the N-terminal ... (1991) Comparative base specificity, stability, and < /b> lectin activity of < /b> two lectins from eggs of < /b> Rana catesbeiana and < /b> R japonica and < /b> liver ribonuclease from R catesbeiana J Biochem (Tokyo) 109,...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
... [151] Formation < /b> of < /b> the oxygenating species has been recognized to be facilitated by the binding to HbO2 of < /b> aniline and < /b> some of < /b> its derivatives, causing distortion of < /b> the iron-oxygen bond to such ... mechanism of < /b> this class of < /b> enzymes Naphthalene 1,2dioxygenase is a heterohexamer composed of < /b> an equimolar combination of < /b> a- and < /b> b- subunits, each a-subunit bearing an Fe2S2 cluster and < /b> a mononuclear ... Ingelman-Sundberg, M & Johansson, I (1984) Mechanism of < /b> hydroxyl radical formation < /b> and < /b> ethanol oxidation by ethanolinducible and < /b> other forms of < /b> rabbit liver microsomal cytochromes P-450 J Biol Chem...
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Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx
... English text This work has been financed by the European Union Network ERB4061-PL-950200 and < /b> by grants PB96-1446 and < /b> BIO2000-1459 of < /b> the Spanish Ministry of < /b> Science and < /b> Technology The Oxford Centre ... contacts between domains A and < /b> B It is surprising that removal of < /b> these interactions does not affect the stability of < /b> domain B Nevertheless, interdomain contacts may be conditioned by the complex formation < /b> ... sequences of < /b> the protein chain [1–63, 274(75))380] Of < /b> these, the N-terminal region 1–63 is both necessary and < /b> sufficient for the formation < /b> of < /b> aggregates and < /b> appears to be the nucleus of < /b> aggregation...
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Báo cáo khoa học: Amyloid oligomers: formation and toxicity of Ab oligomers ppt
... the mechanism of < /b> formation < /b> of < /b> Ab oligomers Possible mechanism of < /b> soluble oligomer formation < /b> and < /b> toxicity The mechanism of < /b> formation < /b> of < /b> soluble Ab oligomer in vivo remains unclear Glabe et al [10] ... 2010 FEBS 1351 Formation < /b> of < /b> toxic Ab oligomers M Sakono and < /b> T Zako Fig Formation < /b> and < /b> toxicity mechanisms of < /b> intracellular Ab oligomers Ab can be localized intracellularly by the uptake of < /b> extracellular ... detected the formation < /b> of < /b> soluble Ab assemblies, rather than fibrils, using an analytical ultracentrifugation technique, and < /b> Lambert et al [12] reported the formation < /b> of < /b> small Ab globular oligomers...
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