... DNA in bovine erythrocytes by slot-blot and insituhybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific insituhybridization ... Boye, M., 1998 Specific detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA insituhybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland, ... for colonization of large intestines of streptomycin-treated mice Infect Immun 67, 6152–6156 [37] Felske, A., 1998 Insitu detection of an uncultured predominant Bacillus in dutch grassland soils...
... (1988) Delineation of individual human chromosomes in metaphase and interphase cells by insitu suppression hybridization using recombinant DNA libraries Hum Genet 80, 224–234 Collins, C., Lin Kuo, ... staining with Giemsa stain is improved (see Note 1) 3.6.1 Staining for GBG- and RBG-Banding Sensitize slides in a Coplin jar for 30 at room temperature in 10 µg/mL Hoescht 33258 in 2× SSC Rinse ... may be achieved in the future by combining FISH and green -fluorescent protein detection or FISH with nonfluorescent protein detection DNA–protein codetection using released chromatin fibers will...
... stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for insituhybridization analysis InSituHybridization (ISH) Insituhybridization was performed following ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor cells was scored as: = negative; = weak; = intermediate; = strong (Figure 1) In case of disagreement...
... using biotin labeled hybridization probes Virology 126: 32 – 50 25 Levis F.A., Griffiths S., Dunnicliffe R., Wells M., Dudding N., Bird C.C., 1987 Sensitive insituhybridization technique using ... and Yukinori Yoshimura, 52001 A sensitive non – radioactive insituhybridization method for detection of chicken IgG - chain mRNA: a technique suitable for detecting of variety of mRNA in tissue ... Procedures Online 3: – Japan 18 Baumgart E., Schad A., Grabenbauer M., 2001 Insitu hybridization: general principles and application digoxigenin – labeled cRNA for detection of mRNAs In Immunocytochemistry...
... mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) vào khuôn chứa mẫu, cố định mẫu cho nằm yên dƣới đáy tách rời Sau đặt khuôn inox chứa mẫu paraffin lên bề ... dịch Blocking buffer 1X, chất blocking dung dịch khóa phân tử lai không đặc hiệu lại Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase (AP), Anti – Digoxigenin kết ... Paraffin (1) Paraffin (2) giờ Sơ đồ 3.1: Quy trình xử lý mẫu trƣớc thực chẩn đoán Tổng thời gian xử lý mẫu: 12,5 Paraffin đƣợc dùng quy trình xử lý mẫu hỗn hợp với sáp ong theo tỷ lệ paraffin :...
... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by insituhybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, ... the increasing sensitivity as compared with hourshybridization protocol Digoxigenin-labeled probe was capable of detecting CHV in MDCK cells infected with 103 TCID50 using overnight hybridization...
... Traditional Chinese Medicine Hospital which are the two main hospitals for the treatment of breast cancer in Guilin city of China The specimens were fixed in 10% neutral-buffered formalin (pH7.4) ... considerable interobserver variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city ... visualization reagent for 30 minutes each, and finally with a 3,3'-diaminobenzidine chromogen solution After a finally wash, the slides were counterstained with haematoxylin [15] Tumor cells with...
... results also showed that dark staining was observed around the nucleus predominately, indicating that β-actin mRNAs existed mainly in the vicinity of the nucleus In order to analyze the correlation ... (a-c) Each square indicates the region of the AFM probe insertion Numbers in lower panels indicate β-actin mRNA quantities detected by the SCN method, and dark intracellular staining indicates distribution ... of insertion with the AFM probes β-actin mRNAs were detected mainly in the vicinity of the nucleus Usually β-actin mRNA is known to exist mainly in the vicinity of the nucleus, and these results...
... for in situ, hybridization has been described elswhere (Gellin et al., 1985), and we added only some modifications concerning the washings after hybridization: the slides were first rinsed in x ... explain the efficiency of insituhybridization This observation confirms the one given by Lin et al 1985 We have also modified the washings after hybridization They are less stringent and, in ... & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon Res 6, 349-360 Lin C.C., Draper P.N & De Braekeleer M (1985)...
... Fluorescence in- situ hybridization: Fluorescence in- situhybridization (FISH) is a technology utilizing fluorescently labeled DNA probes (with a fluorescent dye or a hapten, usually in the form ... show a surprising similarity in all the presenting symptoms To explain this, a hypothesis was proposed that in Xq deleted patients the Xinactivation spreads to tip of Xp, thus inactivating the normally ... these genes escape inactivation in females to maintain a balance with the situation in males In addition, there are other genes on the X chromosome that are not regulated by X inactivation whose...
... Cloning PCR Clone Libraries Sequencing of individual bands Sequencing of clones Southern Hybridization analysis Genetic Fingerprints Adaptation of culture conditions Hybridization analysis Fluorescent ... were performed Insituhybridization of vertical biofilm thin sections clearly indicated that the numbers of probe NEU-stained NH4+-oxidizing bacteria (i.e., Nitrosomonas marina-lineage, Nitrosomonas ... probes, respectively InSituHybridization The previously published optimal hybridization conditions were used for each probe All insitu hybridizations were performed according to the procedure...
... both investigated aerobic biotransformations, indicating that the presence of one or two carboxyl groups does not considerably in uence the substrate acceptance To determine the metabolic Strain ... obviously induced other enzymes that metabolise this substrate in a different way Performing the propynoate biotransformation in 1H2O/ D2O (1 : 1) led to an incorporation of 50% deuterium in all ... P putida strain was about 10 times faster than the spontaneous decarboxylation, indicating the presence of an induced decarboxylase in the organism The accumulated pyruvate was mainly hydrolysed...
... X Ding et al / Journal of Colloid and Interface Science 320 (2008) 341–345 by oxidatively polymerizing aniline using ammonium peroxodisulfate as oxidant (NH4 )2 S2 O8 [23] There, the obtained ... Materials Results and discussion Aniline was obtained from Beijing Xingjin Chemical Factory, and was distilled at a reduced pressure before use DBSA (Anyang Xingya Chem Ltd.) was used as the surfactant ... of the resulting PANI/magnetite nanorods prepared by insitu self-assembly method As shown in Fig 2A, PANI/magnetite nanorods has an unique nanorods structure in shape, ca 80 nm in diameter and...
... Sthapit B and Hodgkin T., 2000 A training guide for insitu conservation on farm Version Rome, International Plant Genetic Resources Institute Quy T D and Hue N.T.N (editors), 2006 Insitu Conservation ... QGCT) bảo tồn insitu (nội vi hay chỗ, điều kiện tự nhiên nơi phát sinh/ sinh sống nguồn gen) exsitu (ngoại vi hay chuyển chỗ, nơi khác với nơi xuất sứ/ sinh sống nguồn gen) Bảo tồn insitu loài trồng ... not yet promoted insitu conservation of crop genetic resources in our country In the paper, some policy, administrative and technical approaches are also proposed Keywords: Insitu conservation,...