flk1 or kdr cells
Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx
... arthritis in mice deficient in B and /or T cells WT To understand the role of B and T cells in antibody-mediated inflammation, mice deficient in either B cells or T cells or both were injected with a ... B and T cells, heterozygous female mice R545 deficient in B cells and T cells were crossed with doubly deficient males, and offspring were investigated for the absence of B cells and T cells by ... were scored for arthritis up to month and were included in the calculations None of the control mice developed arthritis Mice deficient in B cells or T cells were compared with WT mice for statistical...
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Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt
... (Sigma) Cells that exceeded at least twice the mean length of cells before DVC were scored as elongated The proportion of DVC positive cells was corrected by the proportion of elongated cells detected ... samples were vortexed for min, placed in ice for 15 min, vortexed again for and finally centrifuged at room temperature at 8000 g for 15 Acid extracts were neutralized to pH with NaOH and stored at ... For untreated cells, glucose incorporation in the absence of catalase reached a steady state level of about 3.6 lmol [14C]glucose per lg protein after h of incubation UV1treated cells incorporated...
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Báo cáo sinh học: "The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" pdf
... cells and tumors [9,11] Moreover, according to large cohort studies of colon [10,19], lung [9,14] and breast [16,20] cancer patients, low tumor bioenergetic signatures are associated with poor ... for experiments Where indicated, cells were incubated with 10 μM 5-FU for 48h, or μM 3BrP or 100 μM IA for 7h Protein electrophoresis and Western blot analysis Cells were resuspended in lysis ... Tumor Volume 2.5 Figure Metabolic inhibitors effectively promote tumor regression HCT116 cells (107 cells per animal) with low bioenergetic signature (Gcells) were injected into nude mice for...
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báo cáo hóa học:" The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" ppt
... cells and tumors [9,11] Moreover, according to large cohort studies of colon [10,19], lung [9,14] and breast [16,20] cancer patients, low tumor bioenergetic signatures are associated with poor ... for experiments Where indicated, cells were incubated with 10 μM 5-FU for 48h, or μM 3BrP or 100 μM IA for 7h Protein electrophoresis and Western blot analysis Cells were resuspended in lysis ... Tumor Volume 2.5 Figure Metabolic inhibitors effectively promote tumor regression HCT116 cells (107 cells per animal) with low bioenergetic signature (Gcells) were injected into nude mice for...
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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx
... cancer cells selected for resistance to cisplatin, paclitaxel, or doxorubicin Cheryl A Sherman-Baust1, Kevin G Becker2, William H.Wood, III2, Yongqing Zhang2, and Patrice J Morin1,3* Laboratory ... thank the members of our laboratory for useful comments on the manuscript We thank Dr Bingxue Yan for technical help on various aspects of this work This research was supported entirely by the Intramural ... doxorubicin or paclitaxel In contrast, the doxorubicinderived resistant cells (OV90D-6 and OV90D-7) exhibited significant cross-resistance to paclitaxel, and the paclitaxel-derived resistant cells...
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báo cáo hóa học:"Transplantation of selected or transgenic blood stem cells – a future treatment for HIV/AIDS?" pot
... require an expansion or the donor pool by a factor of at least 10 However, this problem is alleviated by the worldwide cooperation of stem cell registries in the Bone Marrow Donors Worldwide and European ... typing information to narrow down the set of potential donors for a given patient, so these http://www.jiasociety.org/content/12/1/10 individuals could be tested for CCR5-delta32 before costly ... biosafety reduced S3 laboratory environment for the transgene introduction Future gene therapy approaches may involve allogeneic transplants, obviating the need for laboratory conditions of high...
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Báo cáo toán học: "Expression and function of micro RNAs in immune cells during normal or disease state" pptx
... downregulated in effector cells compare to naïve cells and increased back in memory T cells [14] Effector T cells originate from naïve T cells following antigen exposure and are considered cells in a high ... lineages, through progenitor cells For simplicity, the progenitor cells are not shown and only four of the main hematopoitic lineages are depicted miRNAs that are reported to be involved in the ... reported to be necessary for the differentiation and function of immune cells are found to be abnormally expressed in both solid and liquid tumors Thus the first miRNA microarray profiling reported...
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Báo cáo y học: "Natural killer T cells and rheumatoid arthritis: friend or foe" ppt
... effect of Vαi NKT cells in CD1d–/– mice, while not affecting joint inflammation in wild-type animals Although several studies have highlighted important immunoregulatory properties for TGF-β1 in experimental ... negative regulator for TGF-β1 for many years, the role of IL-4 reported by Kim et al is unexpected and warrants further investigation [15] Likewise, the precise mechanism(s) by which Vαi NKT cells are ... KRN7000, a novel immunomodulator, and its antitumor activities Oncol Res 1995, 7:529-534 Hammond KJ, Kronenberg M: Natural Killer T cells: natural or unnatural regulators of autoimmunity? Curr Opin...
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Báo cáo y học: "System in biology leading to cell pathology: stable proteinprotein interactions after covalent modifications by small molecules or in transgenic cells" pot
... interpretation errors Immunofluorescence studies Cells grown on glass coverslips were fixed for 10 at room temperature in 4% paraformaldehyde in 0.1 M PIPES (pH 6.8), washed in PBS, and permeabilized for in ... interactions in transgenic cells or animals, and the models are used for drug development The present study shows that the protein-protein interactions in transgenic or immortalized cells are different ... per coverslip) The cells were then washed in PBS, incubated for 10 in cold acetone for additional fixing and permeabilization, and again washed in PBS The cells were incubated for 1.5 h with the...
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Báo cáo y học: "Off-pump or Minimized On-pump Coronary Surgery - Initial experience with Circulating Endothelial Cells (CEC) as a supersensitive marker of tissue damage" pptx
... Cardiac Surgery, Minimised Extracorporeal circulation, OPCAB, Circulating endothelial cells -4- Introduction: For decades coronary artery bypass grafting (CABG) was performed with the use of conventional ... of a generalized inflammatory state and thus having the potential to result in significant morbidity [1] In order to decrease morbidity and mortality associated with coronary surgery, myocardial ... Corp., Somerset, NJ, USA) is associated with superior de-airing, is suitable for both coronary and aortic valve surgery and was shown to improve postoperative recovery, reduce early inflammatory...
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báo cáo khoa học: "More expressions of BDNF and TrkB in multiple hepatocellular carcinoma and anti-BDNF or K252a induced apoptosis, supressed invasion of HepG2 and HCCLM3 cells" docx
... The scores of each tumorous sample were multiplied to give a final score of 0, 1, 2, or 4, and the tumors were finally Page of determined as negative: score 0; lower expression: score ≤ 2; or higher ... positively correlated with tumor progression Secretory BDNF in supernatant of HCCLM3 cells with high metastatic potential were much more than that in HepG2 cells Furthermore, HepG2 and HCCLM3 cells ... few human cancers, supporting growth and survival of tumor cells [23] To explore whether HCC cells express BDNF secretorily, BDNF in the supernatant of HepG2 and HCCLM3 cells was examined by ELISA...
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báo cáo khoa học: "Geometric conservation laws for cells or vesicles with membrane nanotubes or singular points" pdf
... integral theorems lay A A the foundation for the conservation law for cells or vesicles with membrane nanotubes or singular points Figure A cell or vesicle with one membrane nanotube A cell or vesicle ... =1 Ai i =1 ∫∫ KdA ≈ ∑ ∫∫ KdA = −π ∑ mi = A Figure vesicles with one or two singular points Cells or Cells or vesicles with one or two singular points (a) One singular point, (b) Two singular points ... Nanotubular highways for intercellular organelle transport Science 2004, 303:1007-1010 Evans E, Bowman H, Leung A, Needham D, Tirrell D: Biomembrane templates for nanoscale conduits and networks Science...
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Báo cáo y học: " Mesenchymal stem cells in autoimmune diseases: hype or hope" ppt
... studies report amelioration of arthritic symptoms in preclinical arthritis models, whereas other studies – such as that by Schurgers and colleagues – could not report beneficial effects or even describe ... Tuncer HH, Betancur M, Wolfberg A, Klingemann H: Umbilical cord mesenchymal stem cells increase expansion of cord blood natural killer cells Biol Blood Marrow Transplant 2008, 14:1031-1038 Shlomchik ... Apparailly F, Louis-Plence P, Bony C, Sany J, Jorgensen C, Noël D: Reversal of the immunosuppressive properties of mesenchymal stem cells by tumor necrosis factor alpha in collagen-induced arthritis...
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Báo cáo y học: "CD4+ T cells spontaneously producing human immunodeficiency virus type I in breast milk from women with or without antiretroviral drugs" potx
... mononuclear cells stored in liquid nitrogen was performed in the Montpellier laboratory using Abs conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE/RD1), energy coupled dye (ECD), or ... imply that the majority of T cells found in the milk are mainly memory T cells This observation was confirmed by the high level of cell-surface CD45RO receptor expression on these cells (data not ... Gating strategy to explore breast milk CD4+ T cells and CD8+ T cells (B) Analysis of CD38 and HLA-DR cell-surface expression on breast milk CD4+ T cells (left) and CD8+ T cells (right) (C) CD38...
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Dysfunctional signaling pathway for nitric oxide production in endothelial cells chronically exposed to high glucose or high fatty acids
... scenario Exposure of cultured bovine aortic endothelial cells (BAECs) or human umbilical vein endothelial cells (HUVECs) to high glucose or high fatty acids for or 10 days significantly reduced NO ... endothelial cells; this study was thus performed only on endothelium of large vessels, i.e., endothelial cells from aorta or umbilical vein Attenuated availability of NO accounts for the majority of ... inhibitors and antioxidants could reverse high glucose- or high fatty acid-induced impairments on NO formation, [Ca2+]i rise, bradykinin receptor (receptor number as well as affinity), and cell morphology...
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Analysis of the mechanical reaction of human endothelial cells to a single constant or transient uniaxial strain
... analysis, for more than one solution exists for the same dataset: the same displacement field can result in different deformations and forces Moreover, the solution is very sensitive to minor changes ... ImageJ, MATLAB 7.11 (MathWorks, Natick, MA, USA) and Origin 8.1 G (OriginLab, Northampton, MA, USA) Cell force analysis was performed using a MATLAB 7.14 standalone (MathWorks, Natick, MA, USA) ... program for cell force analysis 17 2.4.4 Cell Force Analysis The algorithms for cell force analysis (CFA) were developed in MATLAB and are described in detail in [4] The program was further corrected...
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When the Discussion Gets Stalled or Heated
... Keep power in your pocket (e.g. attorney, credible reference) Keep the door open Conflict as a Choice Point Explore whether conflict is a signal for a change View the disagreement as a choice point and explore options for moving ... Pause when formulating judgment Understand what was meant by actions or words Reflect on information and ask for additional information Reinterpret by applying an alternate explanation Redirect conversation toward ... Dismissive – interrupting, talking over, ignoring {men engage more frequently than women} • Exclusionary – leaving someone out of the meeting, discussion or social gathering • Undermining – going behind the back of others or using insults to discredit arguments...
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Quagmire or Gold Mine?
... make digital/hard copy of part or all of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage, the ... the store’s responses In the software shopping domain, Shopbot was given the home pages for 12 online software vendors Shopbot learned to extract product information from each of the stores, ... in Information Retrieval (Copenhagen, Denmark), June 12, 1992, pp 318–329 Doorenbos, R.B Etzioni, O and Weld, D.S A scalable comparison-shopping agent for the world-wide web Technical Report 96–01–03,...
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