flexibility and function of the escherichia coli trp repressor
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... the recovery of E coli from the stationary phase ACKNOWLEDGEMENTS This paper is dedicated to the memory of the late Professor Alexander Girshovich who initiated and was actively involved in the ... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing...
- 9
- 548
- 0
Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
Ngày tải lên :
21/02/2014, 01:21
... to the theoretical model and in the subsequent simulations [22] the corresponding ratios were KI2/KS2 ¼ and KI1/ KS1 ¼ 0.7 These values and the transition of the Eadie– Hofstee plot from the ... curved shape of the plot and the KI/KS ratios indicate that the C-6 epoxide 1d, like the C-6 aldehydes of Glc and aMGlc [15], preferentially inhibits the low-affinity site of EIIGlc For the phosphorylatable ... lM KS1 and KS2 are the dissociation constants of E1 and E2 for Glc, KI1 and KI2 the dissociation constants for the inhibitor, and k1 and k2 are the turnover numbers DYNAFIT was used to fit the experimental...
- 12
- 720
- 0
Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx
Ngày tải lên :
16/03/2014, 01:20
... representative TTRs, TRPs and TLPs suggests that the three protein groups are not functionally related (Fig 1) The sequence identity between TRPs and TTRs is relatively low; Escherichia coli TRP (ecTRP) shares ... b-strands B and E in TLPs, and to a-helix E in TTRs and TRPs (B) Phylogenetic tree of TRPs, TTRs, and TLPs The tree was based on the multiple sequence alignment from (A) Caenorhabditis elegans TRP- R09H10.3 ... was the low thermostability of the ecTRP protein, with a Tm value approximately 26 °C below the values for both sbTTR and hTTR (Fig 7) The inability of ecTRP to form fibrils at low pH can therefore...
- 13
- 419
- 0
Báo cáo Y học: Refolding of the Escherichia coli expressed extracellular domain of a7 nicotinic acetylcholine receptor Cys116 mutation diminishes aggregation and stabilizes the b structure pdf
Ngày tải lên :
31/03/2014, 23:20
... were shown by Green and coworkers to depend on the state of cysteine residues in the ECDs of the a7 and a subunits, respectively [26,32] The major role of the redox state of the disulfide Cys128–Cys142 ... better than the starting protein (Table 1), and its CD curves are not very sensitive to the conditions of refolding or measuring the spectra Therefore, the conformation of the a7 moiety in the mutant ... similar to the ECD conformation of the intact a7 AChR Although calculation of the secondary structure of the 1–208 fragment from the CD data for the fusion protein GST–a7-(1–208) and GST might...
- 9
- 342
- 0
Nghiên cứu khoa học nông nghiệp " COMPARISON OF THE PATHOTYPES AND VIROTYPES OF PATHOGENIC ESCHERICHIA COLI IN DISEASED PIGS IN VIETNAM AND QUEBEC, CANADA " doc
Ngày tải lên :
21/06/2014, 06:20
... tested by PCR for the presence of defining genes of ETEC and STEC pathotypes They were then virotyped by colony hybridisation (Ngeleka et al., 2002) for detection of the presence of 20 virulence ... regulation and usage between Canada and Vietnam This was definitely the case for tetracycline, ampicillin, and sulfa drug use in both countries, and ceftiofur and apramycin use in Canada, and enrofloxacin ... monitoring the geographical distribution of virotypes worldwide and the emergence of new pathogenic variants We are presently setting up a database for the global surveillance of animal pathogenic E coli...
- 3
- 348
- 0
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21 ... structural and functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule cells Further studies ... 477 and 482) and one Thr residue (amino acid 487) were well conserved in the KIND2 of v-KIND in all species analyzed, although not in the other KINDs To investigate the possible involvement of these...
- 11
- 658
- 0
Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
Ngày tải lên :
06/03/2014, 09:22
... markers are indicated on the left of Spatzle-C108 (Fig 8A) Analysis of tryptic peptides ¨ from these bands by MS ⁄ MS and mascot software identified them as attacin-1, lysozyme, and cecropinA ⁄ B (Table ... yet in other insect species Although it is clear that Drosophila Spatzle acts as ¨ the ligand of the Toll receptor in two important physiological pathways [16,36], the functions of other homologs, ... comparison of the genomic and cDNA sequences Analysis of the amino acid sequences deduced 158 from the cDNA, including prediction of signal peptide and glycosylation sites, was carried out in the expasy...
- 15
- 540
- 0
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
Ngày tải lên :
06/03/2014, 11:20
... on one side of the b-sheet and two on the other side The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the HN of Thr358 and the Ha of His356 could be seen in both conformers (Fig 4; Fig S3) At the same time, the intensity of the NOE between the HN of Phe357 and the Ha of Lys354 in the open conformer ... conformer of the C-domain (red and blue) on the crystal structure [20] of the complex of human eRF1 (green) and the truncated eRF3 (purple) The superposition was made using the Ca, C¢ and N atoms of the...
- 17
- 490
- 0
Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc
Ngày tải lên :
16/03/2014, 16:20
... determined the 3D structure of a : complex of the E coli v and w subunits in order to explore the function of these two proteins Subunits v and w interact to form an elongated heterodimer, of comparable ... responsible for the stable interaction of single-stranded DNA, SSB and the v:w complex The N-terminal segment of w is a possible linker between v:w and the clamp-loader In the crystals of v:w, the N-terminal ... to the c subunit and the b clamp As expected, the c subunit shows a high degree of conservation in the first two domains that make up the AAA+ ATPase portion of the subunit The third domain of the...
- 11
- 363
- 0
Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
Ngày tải lên :
30/03/2014, 01:20
... positioned at the convex side of the b-sheet near to the ends of the b4-strand and b5b-strand Residues in the a4-helix establish a network of contacts with Asp6, His38, Leu35, and Ala142, and undergo ... as the sum of the contributions of free and bound protein: ẵh215 ẳ ẵP ẵhP ỵ ẵPL ẵhPL 1ị where [P] and [PL] represent the concentration of the free and bound protein, respectively, and [h]P and ... located in the reverse turn following the b2-strand, also observed by Weininger et al [31] Furthermore, Ile90 and Ile91, which reside in the loop between the b2-strand and b3-strand of HsFKBP12,...
- 16
- 233
- 0
Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
Ngày tải lên :
30/03/2014, 10:20
... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... density map (Fig 5) The hydrophobic portion of the AB pocket is formed by Trp2 27A, Ile234A and Met117A (the superscript here and below indicates the monomer) The hydrophilic part of the pocket probably ... [14]) The sequences of the CMLE1s from members of the Rhizobiales, such as ArCMLE1 and AtCMLE1, are significantly shorter than the isofunctional enzymes from other bacteria (Fig 2) Expression of...
- 14
- 326
- 0
Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx
Ngày tải lên :
30/03/2014, 16:20
... and 182, and no tryptophans Therefore, the shape of the near-UV CD spectra of EspA suggests the presence of some tertiary contacts around at least one of the tyrosines both at pH 7.0 and 2.0 (Fig ... equilibrium (A) Plot of the logarithm of the absorbance at 280 nm, A280, as a function of the square of the radial distance, r2 Data were collected at pH 7.0 with 1.0 mgÆmL)1 EspA (B) Plot of Mapp vs protein ... cells [16] Although there is information available concerning the roles and structural properties of EspA filaments, to date, the conformation and the thermodynamic properties of EspA have not been...
- 11
- 475
- 0
Báo cáo Y học: Diffusion through channel derivatives of the Escherichia coli FhuA transport protein doc
Ngày tải lên :
31/03/2014, 08:20
... inhibitors and the maltodextrins added to the filter paper discs are indicated The size of the zones of growth inhibition (in mm) of E coli HK97 with SDS and the antibiotics is given with subtraction of ... until the uptake assay was completed (H Killmann and G Gestwa, unpublished data) At the end of the assay, the microtest tubes were cut with a scalpel in the middle of the silicone oil layer and the ... performed to further determine the selectivity of the FhuA mutant proteins After the incorporation of 100–1000 channels into the membranes, the KCl concentration on one side of the membranes was...
- 12
- 418
- 0
structure and function of the arabic verb
Ngày tải lên :
03/04/2014, 12:48
... very indicative, in our opinion, of the adequacy and the basis of this orientation, on the one hand, and the destiny of linguistics, on the other hand Of course, these implications might be too ... constructions The frequency of WA, FA-, LA-, and Ø in front of QAD Frequency of the compound perfect The distribution and frequency of sawfa/saThe frequency of QAD, laʕalla, and rubbamaa The contextual ... STRUCTURE AND FUNCTION OF THE ARABIC VERB Structure and Function of the Arabic Verb is a corpus-based study that unveils the morpho-syntax and the semantics of the Arabic verb Approaches...
- 249
- 1.2K
- 0
Báo cáo y học: "Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the protein" ppsx
Ngày tải lên :
14/08/2014, 14:21
... expressed ones [5-7] We have studied the relationship between the phyletic age of a protein and its expression profile, and related this to the function of the protein The term 'phyletic age' used here ... proteinsthe inanalysis analysis tionalhere assignments of analysis in the analysis analysis FuncFunctional File usedof of thethe proteins analysis analysis Phyletic Additionalforof of the proteins ... table and the test statistics of these were used to form an estimate of their distribution under the null hypothesis, to which the observed test statistic can be compared Additional data files The...
- 9
- 391
- 0
EXPRESSION AND FUNCTION OF THE PRL FAMILY OF PROTEIN TYROSINE PHOSPHATASE
Ngày tải lên :
24/08/2014, 11:44
... transcription factor function The combined results of these studies have expanded our current understanding of the expression and function of the PRL family of enzymes as well as of the role these important ... characterization of the tissues and cell types that express PRL-1 and -2 is important to elucidating the physiological function of the normal genes and to understanding their roles in the pathogenesis of disease ... 2G) Equivalent levels of expression were noted in the villous epithelium, crypt epithelium, and muscularis mucosae of the duodenum and jejunum and in the Brunner’s glands of the duodenum PRL-2 was...
- 325
- 562
- 0
Regulation and function of the novel candidate tumor suppressor gene dlec1 in the HCT116 colorectal cancer cell line
Ngày tải lên :
16/10/2015, 15:37
... associated with the deletion of multiple chromosomal regions including chromosomes 5q, 17p, and 18q Such chromosome loss is often suggestive of the deletion or loss of function of TSGs The candidate ... either have a repressive effect on the regulation of the cell cycle or promote apoptosis, and sometimes both The functions of tumor suppressor genes fall into several categories including the ... followed by the activation of kinases of the Raf family, which in turn phosphorylates MEK 11 INTRODUCTION Phosphorylation of MEK leads to the activation of ERK1/2, which then triggers the downstream...
- 75
- 234
- 0
Tài liệu Báo cáo khoa học: The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC doc
Ngày tải lên :
20/02/2014, 11:20
... translocation The analysis of the membrane insertion of the wild-type KdpD showed that the translocation of the second periplasmic loop is independent of SecA, SecE, and YidC, and is only affected by the ... sequences of each of the two extra-membrane loops before and after the insertion of the epitopes Insertion of the epitopes (underlined) has the following consequences for length (number of amino ... SecYE and YidC, but depends on the pmf, the translocation of the second loop is supported by SecYE and YidC Membrane insertion of split osmosensor fragments The kdpD gene encoding the HA- and the...
- 11
- 532
- 0
Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf
Ngày tải lên :
18/03/2014, 01:20
... the change of the ratio of exported ATP to ADP in the presence of the uncoupler CCCP is remarkable (Fig 3A–C, lane and 6) This holds true particularly for the plant AAC (Fig 3A, lane and 6) The ... between the nonretarded growth of E coli and the energization of the cells, which is necessary for building up the required proton motive force across the membrane Thus, the expression in E coli ... induced E coli cells, then the export of adenylates by the recombinant AACs must depend on the presence of the appropriate externally applied substrate Therefore, we preloaded IPTG-induced E coli...
- 10
- 486
- 0
Báo cáo khoa học: Stabilities and activities of the N- and C-domains of FKBP22 from a psychrotrophic bacterium overproduced in Escherichia coli pptx
Ngày tải lên :
23/03/2014, 13:20
... proteolysis of L pneumophila MIP allows the separation of their N- and C-domains such that the C-domain contains the C-terminal half of the a3 helix [23,24] In addition, the C-domain of E coli FkpA ... consist of N- and C-domains, it would be informative to construct the SIB1 FKBP22 variants containing either one of these domains and compare their activities and stabilities with those of the intact ... composed of N- and C-domains, which are spanned by a 40 amino acid long a3 helix The N-domain consists of a1 and a2 helices and an N-terminal region of a3 helix The C-domain consists of six b-strands...
- 11
- 332
- 0