fips rab11 binding proteins that regulate endocytic sorting and recycling

Báo cáo y học: "Summary The claudin multigene family encodes tetraspan membrane proteins that are crucial structural and functional components of tight junctions, which have important roles in regulating para cellular" pdf

Ngày tải lên : 14/08/2014, 21:20

... residues [11] The region that shows the most sequence and size heterogeneity among the claudin proteins is the carboxyterminal tail It contains a PDZ-domain -binding motif that allows claudins to ... scaf fold ng proteins, such as the TJ-associated proteins i MUPP1 [12], PATJ [13], ZO-1, ZO-2 and ZO-3, and MAGUKs [14] Furthermore, the carboxy-terminal tail upstream of the PDZ -binding motif ... phosphorylation [16] and palmitoylation [17], that can significantly alter claudin localization and function Most cell types express multiple claudins, and the homo typic and heterotypic interactions...

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Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx

Ngày tải lên : 18/02/2014, 16:20

... All lens b-crystallins are calcium -binding proteins M K Jobby and Y Sharma Table Binding constants and the enthalpy change of calciumand terbium -binding to bB2- and bA3-crystallins K, dissociation ... for calciumbinding, suggest that both bB2- and bA3-crystallins bind calcium with moderate affinity We have observed that these proteins lose the calcium -binding ability upon storage and specific ... Demonstration of calcium -binding to b-crystallins would fill an important and missing link in our existing knowledge about bc-crystallins as calcium -binding proteins and understanding their function...

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Báo cáo khoa học: Plant RMR proteins: unique vacuolar sorting receptors that couple ligand sorting with membrane internalization pdf

Ngày tải lên : 15/03/2014, 00:20

... ligand binding [28,29] These experiments provided a reason to hypothesize that RMR proteins themselves might have a function in binding different types of ligands and might also serve as sorting ... ligand -binding domain was contained within the N-terminal unique region, and where the RMR domain contributed to ligand binding [27] The receptor homology domains found within BP-80 and RMR proteins ... digestive vacuole and a vacuole that stored proteins [1,16,31], that the storage vacuole was served by an intracellular pathway different from that trafﬁcked by BP-80 [13,32], and that so-called...

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Tài liệu Báo cáo khoa học: Disulﬁde bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt

Ngày tải lên : 18/02/2014, 06:20

... Tyr9 and Gln11 (strand A), Arg32 (helix II), Val90, Lys92, and Glu94 (strand G), Phe96 and Ser97 (strand H), Phe113 (strand I), and Arg120 and Val125 (strand J) (Fig 7) We can conclude that, ... b-strands and two a-helices in the following regions: 5–8 (strand A), 14–18 (helix 1), 25–29 (helix 2), 34–43 (strand B), 46–53 (strand C), 56–60 (strand D), 66–71 (strand E), 76–85 (strand F), ... signiﬁcant changes occurred at the level of strand E (Ala68, Asp69, and Ile71), strand F (Lys79, Cys80, Thr81, and Leu84), strand G (Ser93), and strand H (His98) (Fig 5) All of the mentioned residues...

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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

Ngày tải lên : 18/02/2014, 08:20

... concentrations Sequence analysis and modelling of LlChi18A and LlCBP33A Cloning and purication of LlChi18A and LlCBP33A The gene fragments coding for the mature proteins of LlChi18A and LlCBP33A were successfully ... [25] and proteins E7 and E8 from Thermobifdia fusca [26], showing a large diversity of binding preferences The function of family 33 CBPs was rst demonstrated for CBP21 L lactis chitinase and ... L lactis chitinase and chitin -binding protein from S marcescens [10], and a second example has been described recently in a study on carbohydratebinding proteins and domains from T fusca [26]...

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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

Ngày tải lên : 18/02/2014, 16:20

... structures (e.g 1CVJ_1 and 1CVJ_2 represent the ﬁrst and second Poly A binding protein domain of structure 1CVJ, respectively), and the two domains were considered structurally and thermodynamically ... function that discriminates the structures of native protein–RNA complexes from decoys, reproduces experimentally determined relative binding afﬁnities for a number of RNA -binding proteins, and predicts ... sequence for ‘orphan’ RNA -binding proteins would greatly facilitate the design of biological experiments aimed at dissecting the function of these proteins It is also a problem that is not well suited...

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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

Ngày tải lên : 19/02/2014, 05:20

... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... active module and a highly conserved choline -binding module (CBM) that allows the binding to phosphorylcholine residues The CBMs are built up of several tandem repeats (cholinebinding repeats, ... 369 Inhibition of choline -binding proteins B Maestro et al Thermal unfolding transition in the absence and in the presence of the different ligands The inﬂuence of ligand binding on the thermal...

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Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

Ngày tải lên : 19/02/2014, 12:20

... mRNA We ﬁnd that CSD proteins form a cytoplasmic complex on VEGF mRNA that also contains the multifunctional singlestrand RNA/DNA binding protein, PTB [43–46,52–57], and that the binding of this ... potential PTB binding sites, cytoplasmic complexes binding to the VEGF 5¢- and 3¢-UTR sites contained both CSD and PTB proteins and it was observed that both the consensus CSD sequence and the downstream ... CSD and PTB proteins may play in directing CCV1 complex binding to the 5¢-ACCUCUU-3¢ and 5¢-UUUUCUU-3¢ sequences, recombinant CSD (GST-dbpB/YB-1) and PTB (GSTPTB) binding to wild-type (V1) and...

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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

Ngày tải lên : 19/02/2014, 16:20

... Ca2+ -binding elements, such as sarcalumenin [30], and the calsequestrin-like proteins CLP-150, CLP-170 and CLP-220 [29], are affected in dystrophin-deficient fibres, this study demonstrates that ... (v/v) acetone and thoroughly broken up by vortexing and sonication The centrifugation and washing step was repeated once and the final protein precipitate collected by centrifugation and resuspended ... electrophoretic separation of muscle proteins prior to immunoblotting and dyebinding analysis For the identification of proteins by MS, Coomassie-labelled protein spots were numbered and no major differences...

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Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt

Ngày tải lên : 19/02/2014, 17:20

... conserved residues that contribute to RNA binding, namely RNP positions 1, and and RNP position (see the following section and Fig 1) The RNP and RNP motifs are located in the central strands of the ... contrary to the well known CCHHs that bind double-stranded DNA or RNA, the CCCH and CCHC zinc ﬁngers are domains that bind single-stranded RNA [9,10] The PABP and the WW domains [11] are protein–protein ... b-sheet (see below [25]) Y14 and Magoh proteins are part of the exon junction complex that comprises several proteins Y14 and Magoh form a highly stable complex with nanomolar binding afﬁnity [48] The...

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Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx

Ngày tải lên : 22/02/2014, 04:20

... whole cell lysate (A and C, lg; B and D, lg), BSA (A and C, lg; B and D, lg), RNA polymerase (A and C, lg; B and D, lg) and RpoD (RNA polymerase r subunit) (A and C, lg; B and D, lg), by SDS/PAGE ... such as membrane proteins, ribosomal proteins and nucleoid-associated DNA -binding proteins (or nucleoid proteins) The overall pattern of Zn(II) binding was essentially the same with that obtained ... newly identiﬁed Zn(II) -binding proteins, AckA and Ppa, and the known metal -binding proteins, Def, MerR and Fur, in the presence of a ﬁxed amount of radioactive Zn(II) and increasing concentrations...

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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx

Ngày tải lên : 06/03/2014, 11:20

... fragments and nonantibody small scaffold proteins from X-ray and NMR analyses enables the design of and screening for small binding proteins The preparation of the small binding proteins with binding ... CDRs of heavy chain and CDR3 of light chain [ref 39] Fv Randomization in CDR loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local ... enables us to obtain valuable binding proteins using selection methodology, and recent structural visualization of candidate proteins by X-ray or NMR structural analyses and the construction of artiﬁcial...

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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot

Ngày tải lên : 06/03/2014, 22:21

... 14-3-3 proteins, global proteomics and biochemical analyses were carried out to identify proteins that bind 14-3-3 proteins during apoptotic and survival conditions These 14-3-3-interacting proteins ... with 14-3-3 proteins were puriﬁed and identiﬁed in this study (Tables and 2) Frequently, 14-3-3 proteins regulate cellular processes by binding to phosphorylated motifs (phosphoserine and phosphothreonine) ... clearly shown that 14-3-3 proteins are survival proteins with antiapoptotic effects in cells, by binding to well-known antiapoptotic proteins and probably also to the apoptosis-related proteins reported...

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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Ngày tải lên : 07/03/2014, 01:20

... Zheng et al Table Role of nuclear actin -binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand -binding domain Actin -binding protein Targeting sequence Gelsolin ... activation function 1, a central DNA -binding domain (DBD) and a C-terminal ligandbinding domain (LBD) containing activation function [67–70] Upon binding androgens, the AR LBD undergoes conformational ... negatively regulates the synergy of FLAP1 and p300 [103] Lee & Stallcup [103] found that Fli-I does not bind well to the p300 KIX domain and does not appear to inhibit FLAP1–p300 binding, suggesting that...

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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence speciﬁc DNA binding proteins pptx

Ngày tải lên : 08/03/2014, 08:20

... sequence boxes I-III (CSB), tRNA genes, L and H strand promoters (LSP and HSP), L and H strand transcription start sites (LSS and HSS) The L-strand sequence and position of the putative promoter ... OL and OH represent the origin of L and H strand replication, respectively LSS, L strand start site; HSS, H strand start site The map positions of the rRNA, tRNAs and mRNAs (ND1 to ND6, A6 and ... speciﬁcity of protein cross-linking Puriﬁcation of D-TERM binding proteins With a view to understand the nature of D-TERM DNA binding proteins and their possible role in transcription Ó FEBS 2003...

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Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt

Ngày tải lên : 08/03/2014, 08:20

... Under the same conditions, phk-1 and phk-2 are not upregulated phk-3 remains inducible in Dif, dorsal, spaetzle and kenny mutant flies [4], suggesting that it is not regulated by the Toll or Imd 3404 ... expression of both phk-2 and phk-3 is strongly reduced by ecdysone in vivo [41] These data support the hypothesis that Phk-2 and Phk-3 may interact with ligands different from Phk-1, and carry other functions ... fluorescence in noninfected larvae in both anterior and posterior spiracles, in the digestive tract, the ring gland, the antenna buds and testis (Fig 3A,B and data not shown) In adult flies, GFP was expressed...

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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modiﬁes the expression level of a limited number of proteins in Escherichia coli doc

Ngày tải lên : 16/03/2014, 00:20

... B=MICB Þ=n where A and B are the MICs of drug A and drug B in the combination, MICA and MICB are the MICs of drug A and drug B alone, FICA and FICB are the FICs of drug A and drug B and n is the number ... b-galactosidase (Fig 6), calcein and dextran release from liposomes mimicking the E coli IM (Figs and 9) and electron microscopy images (Figs 10 and 11) suggest that Esc(1–18) is a membrane-active ... Note that only those spots with a CV £ 5% were considered to be ‘valid’ spots for the performance of differential analysis Spots that were at least twofold upregulated or downregulated and with...

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Báo cáo khoa học: Photosynthetic acclimation: Structural reorganisation of light harvesting antenna – role of redox-dependent phosphorylation of major and minor chlorophyll a/b binding proteins pot

Ngày tải lên : 16/03/2014, 06:20

... [24,44] It now seems that the phosphorylation of LHCII proteins is regulated by a complex network involving co-operative redox control both via PQ and the cyt 6f complex, and through the thioredoxin ... recognition proposes that phospho-LHCII exhibits different binding specificity for both photosystems in that the phosphorylation of the mobile LHCII decreases its affinity for PSII and increases its ... becomes over-reduced Binding of the PQH2 to the quinol -binding site of the cyt b6f complex activates a specific thylakoidbound kinase that directly interacts with the cyt b6f complex and phosphorylates...

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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt

Ngày tải lên : 17/03/2014, 23:20

... Conﬁrmation that MREa -binding proteins are related to the Ku proteins (A) Immunoblot analysis of proteins eluted from the avidin– agarose beads as described in Fig 3B Both the 70- and 82-kDa proteins ... Ku-70 and Ku-80 revealed that the MREa -binding proteins described herein and the Ku proteins are immunologically related The Ku protein complex is known to contain equimolar amounts of the 70- and ... MREs and the Sp1 binding element, and the mutated bases are indicated by italic type) Western blot analysis The MREa -binding proteins puriﬁed from HepG2 cells by the avidin–biotin method and lysates...

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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

Ngày tải lên : 18/03/2014, 01:20

... shown on the left Lanes and show ablomin (26 and 29.7 kDa), lanes and show triflin (23 and 29 kDa), lanes and show latisemin (28 kDa each), and lanes and show tigrin (28 and 30 kDa) The numbers ... specificity and mechanism of action Isolation and characterization of homologous proteins, triflin and latisemin In subsequent experiments, we isolated two other homologous proteins and cloned ... factors that cause partial inhibition The structural alignment appears to show that these proteins are classified into the CAP family of proteins (CRISPs, Antigen proteins, Pathogenesis-related proteins) ...

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