... expanded with and without FGF-2 stained strongly for CD13, CD44, CD90, and CD105 (markers for mesenchymal stem cells) and for CD29 (β1 integrin) (Figure 3) The cells stained poorly for LNGFR and ... IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cellsand of epitopes commonly abundant on adult stemcellsderived ... stemcells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells...
... marrow -derived MSCs Flow cytometric analyses of bone marrow -derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone marrow -derived ... expression and enchance cells survival Kortesidis and colleagues also demonstrated[31] that bone marrow derived- MSCs express factors that support cell survival and avoid apoptosis thereby preserving cells ... aspiration and expanded ex vivo before implantation Under specialized culture conditions, bone marrow -derived MSCs have the capacity to differentiate into cells such as bone, cartilage, adipocytes and...
... the staff of Miplant Stems Clinic Authors’ contributions JP was in charge of patient treatment and follow-up, was responsible for manuscript drafting and revision, and read and approved the final ... range of motion and tenderness with flexion Apley and McMurray tests were negative, and there was no ligament laxity A pre-treatment 1.5T MRI scan demonstrated a decreased size and deformed contour ... range of motion and tenderness with flexion Apley and McMurray tests were negative, and there was no ligamentous laxity A pre-treatment 1.5T MRI demonstrated a decreased size and deformed contour...
... inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human leukemia ... Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004, ... on to fresh feeder cells for confirmation and further analysis; no cells were frozen This work carries forward a theoretical approach suggested more than a decade ago [12], and demonstrates that...
... GK Collection and assembly of data AD Collection and assembly of data AK Data analysis and interpretation, collection and assembly of data CP Conception and design, data analysis and interpretation ... Conception and design, provision of patients, data analysis and interpretation, manuscript writing PA Conception and design, data analysis and interpretation, manuscript writing HA Data analysis and ... implanted cells respond to their environment by secreting cytokines and growth factors which act both in an autocrine fashion on the donor cellsand exert paracrine effects on the host cells [18]...
... Chen SH, Pan PY: Development and function of myeloid -derived suppressor cells generated from mouse embryonic and hematopoietic stemcellsStemCells 2010, 28:620-632 Poschke I, Mougiakakos D, Hansson ... small cell lung for CD11b+) showed an abundance of immature cells, including metamyelocytes or band cellsand blast-like cells (middle left and far right panels, representative images shown of three ... regulatory T cellsand Page 20 of 20 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 CD14+HLA-DR-/low myeloid -derived suppressor cellsand decreased level of dendritic cells in patients...
... cancer stemcells from different cancer tissues and cell lines [16,17] Qverexpressed MUC4 and control cells were stained with FITC-conjugated CD133 cells to analyze the percentage of the cancer stem ... CD44, CD24, ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stemcellsand cancer stemcells are known ... population of cells having different phenotype Side population and Non-side population in MUC4 transfected SKOV3 cells Recently, cancer stemcells have been identified as a minor population of cells...
... source are adult stem cells, in particular, bone-marrow -derived mesenchymal stemcells (MSCs) The use of these cells would allow an autologous approach, reducing the risk of rejection and infection ... phenotype and direct the cells to form a new and appropriate matrix Materials and methods Mesenchymal stem cell source and extraction Bone marrow samples were received from two patients (aged 66 and ... identification of GFP-positive cellsand immunopositivity for matrix proteins in the same cells Results Identification of injected mesenchymal stemcells No GFP-positive cells were observed within...
... CD105 (markers for mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker ... mesenchymal stemcells Science 1999, 284:143-147 Khan WS, Andrews JG, Hardingham TE: The role of FGF-2 in the proliferation and chondrogenic potential of infrapatellar fat pad derivedstemcells [abstract] ... markers on bone marrow -derived stemcells is reported to decline with culture [22-25] In preliminary work the cells have shown osteogenic [9] and adipogenic (W.S Khan and T.E Hardingham, unpublished...
... during injury, stemcells can adopt this form of cell division to expand and replenish the depleted stem cell pool (Morrison and Kimble, 2006) 1.4 1.4.1 Types of stemcells Embryonic stemcells During ... maintain these cells in an undifferentiated, quiescent state For stemcells to be activated, the niche environment must change (Fuchs, 2007) 1.4.3 Stemcellsand cancer Stemcellsand cancer cells share ... The stem cell concept 1.3 Symmetrical and asymmetrical division 1.4 Types of stemcells 1.4.1 Embryonic stemcells 1.4.2 Somatic stemcells 1.4.3 Stem...
... of understanding the functioning of these remarkable stemcells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stemcells are ... germ line); these stemcells exhibit high rates of turnover, undergoing continual self-renewal and production of differentiated progeny; and neoblasts are the only proliferative cells in the body ... Deuterostomia, Lophotrochozoa, and Ecdysozoa) Whether their similar stem- cell systems are homologous or convergent remains an open question The biology of these stemcells is currently being investigated...
... changes of SWD values along the stem vertical profile, (c) to investigate changes of SWD with respect to stand density and thinning MATERIALS AND METHODS Site and stand description: There were two ... 2004 Stem wood density (kg/m ) As Ad Es Ed Fig Stem wood density in subvariants in elevated (E) and ambient (A) concentration of CO2 and sparse (s) and dense (d) subvariant within vertical stem ... Variant sparse dense Fig Average stem wood density in elevated (E) and ambient (A) concentration of CO2 and sparse (s) and dense (d) subvariant independent from vertical stem profile; (a) 2002, i.e...
... classification for stem sinuosity and another one for stem lean In “Sierra de Oña” provenance stem sinuosity in mature trees is very sharp, being an own character more outstanding than stem lean In ... environmental attributes (altitude, slope and exposure), stand attributes (N, BA, Dg, H0 and SI), individual tree attributes (DBH, DB, HT, A and BAL/BA) and upon the stem straightness Multinomial logistic ... young stand is still very young and the social differentiation has not clearly started In this way it could be interesting to study the relationship between stem straightness and tree and stand attributes...
... calculated on mid -stem and breast height cuvettes, whereas the averages for rG and RG%, respectively, were 0.13 and 53.9% at breast height and 0.27 and 75.9% at mid -stem Both rG and RG% had higher ... (percentage of living cellsand nitrogen concentration in the wood, stem temperature and growth) and their influence on stem respiration throughout the year, between trees and within trees; (2) ... coefficient (rG) and biomass increments, of standing biomass and maintenance respiration rate (rM), are required to estimate stem respiration at stand level Usually, rG and rM are derived from measurements...
... Lesprojekt, Brandys n/L., 1984, 34 p (in Czech) [33] Prax A., The study of soil and ground water and of total cycle in the forest and meadow ecosystems of floodplain and hilly regions and pure spruce ... the stem respiration (mg CO -1 ·h -2 ·dm R the stem respiration of maintenance (mg - ) -1 ·h -2 ·dm CO stem radius p S s S - crown at stand S = ) (dm 10 000 m unit of stand area of part stand ... radiation balance (I andstem radius (dr) recorded ) every 12 h (at 06:00 and 18:00 hours) were available for most of the growing season Daily means of air temperature and air humidity and daily precipitation...
... with ESR and CRP and IgM-RF also had a low correlation with ESR and CRP, although the latter correlation was slightly higher both at a single time point and in the course of time Matsui and colleagues ... network in The Netherlands Materials and methods ACPA and IgM-RF levels were determined in 22,427 samples, which were collected from 18,658 patients between August 2003 and August 2007 These ... disease, and undifferentiated spondyloarthropathy), osteoarthritis, and other (including arthralgia, fibromyalgia, and no final diagnosis) The latter four groups were also combined and classified...
... controls and (d to f) systemic sclerosis (SSc) patients were stained with anti-human-CD68, CD163 and CD204 antibody, respectively A larger number of (d) CD68+ cells, (e) CD163+ cellsand (f) CD204+ cells ... perivascular and periappendageal infiltrated macrophages (Table 2) There was no difference in the number of CD68+ cells between healthy controls and SSc patients However, the number of CD163+ cellsand ... envelope and Golgi apparatus of non-monocyte lineage cells but moved to the cell surface and endosomes of monocytes lineage-like cells [24] MFI of CD163 in CD14+ PMBCs of both lcSSc and dcSSc...