features comparison to existing est datasets and analysis of transcriptome complexity and differentiation
Báo cáo y học: "Serum-dependent transcriptional networks identify distinct functional roles for H-Ras and N-Ras during initial stages of the cell cycle" doc
... G2/M Apoptosis Apoptosis = 1,86% -/H-ras G1 Apoptosis G2/M S Apoptosis Apoptosis = 1,13% Apoptosis = 1,06% G1 G1 S Apoptosis Apoptosis Apoptosis G2/M Apoptosis = 3,09% N-ras -/- G2/M S Apoptosis ... microarraybased analysis of the transcriptomic profiles of the serum starved, G0-arrested fibroblasts enables the participation of the Ras isoforms in cellular responses to the stress of serum deprivation to ... instances the comparison of the profiles of G0-arrested WT cells with those of the other samples and conditions studied here by means of microarray hybridization Interestingly, the comparison of the...
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báo cáo khoa học: "Transcript profiling reveals expression differences in wild-type and glabrous soybean lines" pdf
... glabrous mutant into Clark for six generations [27] Total RNA isolated from shoot tips of both CS and CG plants was analyzed by Illumina DGE tag profiling to create transcriptome profiles of the two ... respectively At a cutoff of RPKM, however, 41,972 and 44,120 genes were expressed in CS and CG, respectively Together, the results suggest that in the RNA-Seq transcriptome, ~50% of genes are expressed ... sensitivity of RNA-Seq depends on the transcript length [42] RNA-Seq analysis revealed that at the cutoff point of 10 RPKM, a total of 11,574 and 14,378 genes were expressed in CS and CG, respectively...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... visualization of structure and dynamics of biomolecules and STO – A program for computing stochastic paths In Modeling of Biomolecular Structure 4069 Effect of A .C mismatch in PNAÆDNA and DNA duplexes and ... C- and N-termini of the PNA are considered as equivalent to 3¢ and 5¢ ends of a nucleic acid chain, respectively Results For convenience of discussion, and to be consistent with the strategy of ... occasionally, C5-H group of cytosine (C8) overlaps with C7 and, O2 of C7 overlaps with C8 On the other hand, sustained stacking persists by way of partial overlap of T7 and C8 (Fig 2F) at the corresponding...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro transcription and translation ... reinhardtii [15] and to determine the location of PsbH in photosystem II (PSII) of C reinhardtii [16] We now report the expression of His- and Strep-tagged plant PSI Results A series of cassettes ... resistant to trypsin digestion under the standard conditions commonly used to determine the topology of thylakoid membrane proteins To find out whether the in vitro behaviour was anomalous, a range of...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
... site of MSOX [12] and of DAAO [15,16] (C) Comparison of the active site of the 3D-structure model of GO with the active site of MSOX from Bacillus subtilis (right) [12] and DAAO from Rhodotorula ... sequences of SOX (A) and DAAO (B) and comparison of the 3D structure of the active site with those of other enzymes (C) Residues marked with (*) indicate identity (:) indicate strongly similar, and ... concentrated and stored at )20 °C HisGO preparation, expression and purification The GO DNA obtained by EcoRI digestion of the original pT7-GO plasmid (see above) was inserted into the EcoRI site of the...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
... lengths: lauroyl-ACP corresponds to an acyl-ACP of length 12, myristoyl-ACP corresponds to an acyl-ACP of length 14, and palmitoleoyl-ACP corresponds to a cd3dACP of length 16 Metabolites encircled ... requirements in terms of moles of ATP hydrolyzed and NADPH oxidized per mole of product of interest produced is given in Table Key enzymes of lipid biosynthesis and contribution to metabolic capacity ... see [14–16,57] Isolation and analysis of genes of the L-form mutant strain E coli LWF1655F+ Amplification of the genes of interest (Table S6), mutation detection and analysis were essentially...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... that only a small fraction of bovine PBMC was susceptible to MYXV infection An average of 1.2 % and 0.8 % of GFP-positive resting cells was detected for T1-Serp2-GFP and SG33-GFP respectively ... FACScalibur (Becton Dickinson) Dead cells and debris were excluded by appropriate gating and 30000 events were collected Analysis was performed using CellQuestPro and Flowjo Software A Cells ... determined by restricted expression of a unique virus receptor J Virol 2005, 79(16):10397-10407 Carroll MW, Moss B: Host range and cytopathogenicity of the highly attenuated MVA strain of vaccinia...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... that only a small fraction of bovine PBMC was susceptible to MYXV infection An average of 1.2 % and 0.8 % of GFP-positive resting cells was detected for T1-Serp2-GFP and SG33-GFP respectively ... FACScalibur (Becton Dickinson) Dead cells and debris were excluded by appropriate gating and 30000 events were collected Analysis was performed using CellQuestPro and Flowjo Software A Cells ... determined by restricted expression of a unique virus receptor J Virol 2005, 79(16):10397-10407 Carroll MW, Moss B: Host range and cytopathogenicity of the highly attenuated MVA strain of vaccinia...
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báo cáo khoa học: " Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein" pps
... Tables describing WT and npq1lut2 transcriptome Additional file 2: Figures describing WT and npq1lut2 plant photosynthetic characterization, transcriptome analysis and transcriptome validation Acknowledgements ... F, Leister D: Analysis of 101 nuclear transcriptomes reveals 23 distinct regulons and their relationship to metabolism, chromosomal gene distribution and co-ordination of nuclear and plastid gene ... mutant and repressed in wild-type plants (Table 5) It is well known that the transcription and the translation of PSII and PSI genes is extremely complex and often uncoupled Analysis of the barley...
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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
... magnification of the ALZ in (M) and (N), respectively Seeds in the first (most mature) pod and close to pea stock are designated as 1.1 The youngest pod and close to the pea stock is designated as 8.1 ... compared to the wild type, may provide valuable insights into elucidating and understanding the biochemical and structural basis of the abscission process [26] Such mutants have been described and ... inheritance and the name and gene symbol (Def) for the locus of development funiculus [37-39] The locus has been found to be located on the bottom end of linkage group VII corresponding to chromosome...
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Tài liệu Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays doc
... factors and cytokines, G-CSF modulates cytokine profiles that alter the composition and function of immune cell populations The serum levels of G-CSF and GMCSF are often elevated in response to ... factors and underlying diseases Additionally, data regarding cytokine profiling in the elderly have been limited to a small subset of cytokines In this study, we compared the profiles of 22 cytokines, ... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... A combination of HPLC and ESI MS allowed the separation of a and b anomers and all chitooligosaccharide products to be monitored simultaneously At the initial stage of reaction and low temperature, ... addition of acetic acid to 10% and then applied to HPLC-ESI MS For calibration of the HPLC peaks (a and b anomers) recorded at different masses in the single ion mode, mixtures of the same chitooligosaccharides ... quantities (< 20% of the dimers at 15 of reaction) and were degraded into dimers and monomers towards the end of the reaction No monomer was detectable at the very early stages, but 20% of monomers...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... We would like to suggest the STD NMR technique, which analyzes, in the dissociated ligand, the magnetization transferred from protons of the protein to protons of the bound ligand that are in ... annealing and elongation time of and an annealing temperature of 55 °C We used the following pairs of flanking and mutagenic primers, where Eco91I and XagI restriction sites are underlined and the ... affected by addition of the same concentration of imidazole (data not shown) 0 100 300 200 400 imidazole (mM) 500 Phosphorus NMR of pyridoxal 5¢-phosphate 31 Fig Analysis of restoration of activity in...
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Báo cáo khoa học: Alternative substrates for wild-type and L109A E. coli CTP synthases Kinetic evidence for a constricted ammonia tunnel doc
... CTPS, and saturating concentrations of UTP (1 mM) and ATP (1 mM) in a total volume of mL Enzyme and nucleotides were preincubated together for 2.5 at 37 C followed by addition of substrate to initiate ... analogues of exogenous NH3 (i.e NH2OH and NH2NH2) and nascent NH3 (i.e NH2OH and NH2NH2 derived from the hydrolysis of Gln-OH and Gln-NH2, respectively) to test our hypothesis that the presence of an ... a freshly prepared stock solution was stored on ice and added cold to each assay This protocol was necessary to minimize cyclization of Gln-OH with concomitant production of NH2OH (see above)...
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Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A ppt
... maximum and quantum yield are sensitive to the microenvironment of tryptophan side-chains in a protein, and both parameters can be used to monitor structural changes and the unfolding of proteins ... (Fig 3B and D), indicative of the release of the substrate Comparison of the Cel7A unfolding with and without the substrate either at pH 8.0 (Fig 3A and B), or pH (Fig 3C and D) shows interestingly ... dichroism and optical rotatory dispersion of proteins and polypeptides Methods Enzymol 27, 675–735 21 Eftink, M.R (1995) Use of multiple spectroscopic methods to monitor equilibrium unfolding of proteins...
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Báo cáo sinh học: "Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays" pptx
... factors and cytokines, G-CSF modulates cytokine profiles that alter the composition and function of immune cell populations The serum levels of G-CSF and GMCSF are often elevated in response to ... factors and underlying diseases Additionally, data regarding cytokine profiling in the elderly have been limited to a small subset of cytokines In this study, we compared the profiles of 22 cytokines, ... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and...
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o cáo hóa học:" Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays" ppt
... factors and cytokines, G-CSF modulates cytokine profiles that alter the composition and function of immune cell populations The serum levels of G-CSF and GMCSF are often elevated in response to ... factors and underlying diseases Additionally, data regarding cytokine profiling in the elderly have been limited to a small subset of cytokines In this study, we compared the profiles of 22 cytokines, ... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and...
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Báo cáo y học: "High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans." potx
... powerful new tools for transcriptome analysis [10-14] Both rely on the availability of high quality genome sequence, and both offer the promise of transcriptome analysis at unprecedented depth and efficiency ... changing transcriptome across development and our immediate goal was to map the wild-type transcriptome at good coverage and resolution and, thus, to provide a framework to analyze perturbations of ... resolution, high coverage 'reference transcriptome' for C elegans to use as a tool to guide analysis of perturbed transcriptomes such as those of mutant animals At the time of initiating these studies,...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... application of the first-round PCR, primers P2 and P4, and P3, P4, P5 and P6 together, were used to amplify the vaccine and wild-type strains, respectively, at different anneal temperatures According to ... phylogenetic and molecular evolution analysis on complete sequences of all CDV genes to assess the role of selection and recombination in shaping viral genetic diversity and driving the emergence of CDV ... T: Comparison of the immunofluorescence assay with RT-PCR and nested PCR in the diagnosis of canine distemper Vet Res Commun 2005, 29:347-59 10 Li JZ, He HB, Xia XZ, et al.: Establishment and...
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Báo cáo y học: "Comparative whole genome sequence analysis of wild-type and cidofovir-resistant monkeypoxvirus" doc
... domain and the outside medium and is thought to mediate NTP entry and selection and support exonuclease proofreading [40] The funnel domain may mediate binding RNA cleavage stimulatory factor TFIIS ... hydrophobicity, and increases motif surface exposure (Table 2) The extent of homology of poxviral rpo147 and rpo30 with cellular RNA pol II Rpb and TFIIS [38,47] suggest general features of their interaction ... Genome comparison The MEGA 4.0 software package [66] was used for SNP/ indel identification and whole genome sequence comparisons of CDV-R and WT Zaire 79-005 The genome of the seed stock used...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25