... CREB and AP -1 seem to be importantdeterminants of Cyr 61/ CCN1 promoter activity and theÓ FEBS 2003 Regulatory mechanisms of the Cyr 61/ CCN1 gene (Eur. J. Biochem. 270) 3 419 Regulation ofCyr 61/ CCN1gene ... Pd98053 and worthmanin. Cells were incubated with the indicated inhibitors asdescribed in (A) and further incubated with either PMA (10 lM)orS1P (10 lM) for 15 min. P-Erk1/2 and Tot-Erk1/2 ... Cyr 61/ CCN1 gene at both the mRNA and protein levels.Cyr 61/ CCN1 mRNA stability was not altered but thetranscription rate of the Cyr 61/ CCN1 gene was increasedfivefold in isolated nuclei from S1P-stimulated...
... se Icdwith a )16 .18 86, b 7 .10 69, c 1. 414 1, d )1. 0 819 ,short dash, aLk a b 1 eÀtopoisomerase IÀcdwith a )17 3. 219 ,b 16 3.529, c ) 5.2409, d 1. 6430, long dash, aLk ... Chem. 27 5, 810 3± 811 3.6. Menzel, R. & Gellert, M. (19 83) Regulation of the genes for E. coliDNA gyrase: hom eostatic control of DNA supercoiling. Cell 34, 10 5 11 3.7. Wang, J.C. (19 71) Interaction ... 5Â-CGAAGAAGGGCGGGGAGAAAT-3Â +bp187 018 50, i.e. 5Â-TCCATAGCAGCGGCGAAACCA-3Â and chromosomalDNA from strain LM1237 [17 ] as a template. The PCRfragment was subsequently digested with the enzymes DraI and EcoRV and a 842-bp...