... that, although the amount of XRCC1 remains the same in mock or Pol b-depleted cell extracts (Fig 3D), cross-linking of XRCC1 in the latter was reduced by approximately twofold (Fig 3E,F), indicating ... lesions as a strand break, verification of the nature of the 3¢ end, and identification of a satisfactory pathway for repair The XRCC1 component of the XRCC1–DNA ligase IIIa heterodimer is thought to ... immunodepletion of PNK did not affect the amounts of XRCC1 and Pol b in the extract (Fig 5B) Using the same extract, we found efficient cross-linking of XRCC1 to the 3¢-OH gapped substrate (Fig 5C)...
... the exact relation The region between netic XXX bemg lkX= 1.00202A worth while to review briefly some properties of electromagnetic Suppose a monochromatic beam of x- rays, i.e., x- rays of a ... APPENDIX VALUES OF APPENDIX QUADRATIC FORMS OF MILLER INDICES APPENDIX VALUES OF APPENDIX ATOMIC SCATTERING FACTORS siN 469 (SIN 0) /X 471 472 474 477 * APPENDIX MULTIPLICITY FACTORS ... the wavelengthof the x- rays Integration of Eq (1-9) gives 4- where /o mitted = - intensity of incident x- ray beam and Ix after passing through a thickness x beam (1-10) /or**, = intensity of...
... f12 x; ZÞ ¼ f11 x1 ; ZÞ expðÀb2 x1 À x ; xx x2 ð74Þ F1 x; ZÞ ¼ f13 x; ZÞ ¼ f12 x2 ; ZÞ exp½Àb3 x2 À x ; xx x3 > h ib4 > > : f14 x; ZÞ ¼ f13 x3 ; ZÞ x ; xx x4 x3 where < f21 x; ZÞ ... where < f21 x; ZÞ ¼ a expðÀb1 x þ ðZ À aÞ expðÀcxÞ; x x1 xx x2 F2 x; ZÞ ¼ f22 x; ZÞ ¼ f21 x1 ; ZÞ exp½Àb2 x1 À x ; : f23 x; ZÞ ¼ f22 x2 ; ZÞ exp½Àb3 x2 À x ; xx x3 Z ð75Þ where Z 100 ... 1972): ij VX ¼ NX 1 ð30Þ VX ¼ NX þ f X NX 2 12 VX ¼ NX þ f X NX 23 þ ðfX 13 þ fX 12 f X ÞNX 23 " In an alternative approach, the mean fluorescence yield oX is a linear combination of the relative...
... description of the strain factor as a function of the structure type and cluster size is aX (n) = X + X − X × π + atan n0 − n /wX X , π + atan n0 − /wX X (5) Table 1: Values of parameters used in ... 1.0 1.0 0.5 5000 XRD intensity (AU) IX,n (q) = A NX (n) + X, n sin 2πqui, j aX (n) X= I 5.0 0.3 4.0 1.0 1.0 0.5 6000 given in terms of the four parameters [n0 , X , X , wX ] InX tensities scattered ... distribution fX (n) = exp − sX /2 2π X sX exp − log n − log X 2s2 X , (4) M Ladisa et al with mode X and logarithmic width sX Structural distances for the different structure types X are generally...
... diagnosis using scattered X- rays J Synchrotron Rad 2000; 7: 348-352 Malden CH, Speller RD, et al A CdZnTe array for the detection of explosives in baggage by energy dispersive X- ray diffraction signatures ... it is expected different peak positions will exist for carcinoma and fibrocystic changes Our results confirmed this expectation The peak position of fibrocystic change was close to that of carcinoma ... Keyrilainen et al Conclusion Measurements of coherently scattered X- ray photons can be used to identify breast disease In this study using diffraction profiles from EDXRD, distinct peak positions were...
... II melt of Form II Form I to Form II melt of Form II loss of water melt of Form I Form I to Form II melt of Form II loss of water melt of Form III melt of Form VI loss of water melt of amorphous ... Figure 30 DSC of mixture of hydrated Forms I and IV (sample 8) Figure 31 DSC of mixture of Form III monohydrate and Form IV hydrate (sample 56) Figure 32 Superimposed XRPD of mixture of Form III ... loss of water melt of Form III? loss of water melt of Form III Form I to Form II melt of Form II loss of water melt of Form III Form I to Form II melt of Form II Form I to Form II melt of Form...
... pantoate-bound form of nPS using X- ray crystallography, as described below A Solution and refinement of the structure using X- ray crystallography B The crystal structure of the pantoate–nPS complex was solved ... this stretch of residues was therefore not rebuilt X- ray crystallographic structure of nPS bound to pantoate Fig The absolute value of deviation in chemical shift (p.p.m.) of residues of nPS upon ... consists of two water molecules The backbone carbonyl oxygen of Phe56 is also hydrogenbonded to the O4 atom of pantoate via one of the water molecules in the same network The phenolic oxygen of Tyr71...
... crystal structures of these Ec-RNase HI variants, the position of His124, which corresponds to His126 of So-RNase HI, varies for different proteins, because of the intrinsic flexibility of the loop containing ... Destabilization mechanism of So-RNase HI A combination of the six thermostabilizing mutations increases the stability of So-RNase HI by 28.8 °C in Tm and 27.0 kJÆmol)1 in DG(H2O) Five of the six substituted ... the aIII and aIV Fig Stereoview of the three-dimensional structure of 6·-RNase HI The structure of molecule D of 6·-RNase HI (gold) is superimposed on the structure of the wild-type protein (green)...
... in the X- ray structures of CBM4 and CBM17 complexed with cellopentaose and cellohexaose, respectively [13,23] The involvement of the tyrosine residues in the stabilization of the complex cannot ... relaxation) of cellohexaose resonances upon addition of CtCBM11 In general, a progressive line broadening of all the cellohexaose protons was observed during titration with increasing amounts of ... Fig Line broadening studies (I) Spectral assignment of 1H NMR cellohexaose resonances (II–IV) Series of spectral regions of a solution of cellohexaose 0.787 mM in D2O, corresponding to protons aa1,...
... of the structure of AII in solution with the X- ray structure of AII complexed to the Fab131 mAb In Fig 7A, a sequence alignment is represented of the backbone of the conformational ensemble of ... side chains of Val3 and Pro7) (a) with the the X- ray structure of AII in the Fab131–AII complex [30] (b) exclude solvent by filling the cavity The most buried residues of AII are of the central ... between the X- ray structure of AII and the ensemble of 13 AII calculated structures Superposition is based on sequence and structure alignment Residue number range in the X- ray structure of AII Fig...
... concentration range of 30–150 lM (1.3–6.5 mgÆmL)1), 90 lL of arrestin solution was mixed with 10 lL of 10 mM peptide solution to yield a final peptide concentration of mM For higher concentrations of arrestin ... Determination of binding constants from forward scattering The intensity of Ôforward scatteringÕ, or I(0), is the X- ray solution scattering that is parallel to the incident beam, and was determined by extrapolation ... Because of the changing oligomeric state of arrestin in these experiments, Guinier analysis was preferred over the use of the distance distribution function, which requires a prior estimation of the...
... states of the CTXS-Xyl2-Me complex were not detected with [S-Xyl2-Me]initial ẳ 5200 lM) Protein (modications) Inhibitor Mexp (Da)a TRX I (none) None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me ... calorimetry of both domains decreased in the series b-d-xylohexaose (Xyl6) > b-d-xylopentaose (Xyl5) > b-d-xylotetraose (Xyl4) > b-d-xylotriose (Xyl3), with no detectable afnity for b-d-xylobiose (Xyl2), ... the expected m z for any charge state of CTXS-Xyl2-Me complex were not detected within [S-Xyl2-Me]initial ẳ 5200 lM) Protein Inhibitor Ka ã 10)3 (M)1) TRX I S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me...
... concentrations of % lm were used Peroxidase activity was assayed using hydrogen peroxide and guaiacol (O-methoxyphenol, Sigma) The synthesis of the fourfold oxidized product of guiacol, 3,3¢-dimethoxy-4,4¢-biphenoquinone ... R Worrall et al X- ray and unfolding studies of cyt c-550 Fig Close up of the heme binding pocket of (A) wt and (B) the M100K(rt) variant of ferricyt c-550, with the positions of the buried water ... unfolding of the M100K variant involves the breaking of the Lys-Nf-iron bond coupled with the dynamic process of ligand exchange It thus seems likely that the mechanism of release of the axial ligand...
... Foundation of China We are grateful to Prof Li-Wen Niu, Prof Mai-Kun Teng and Dr Xue-Yong Zhu of the University of Science and Technology of China for their support and help with the X- ray data ... hydroxyl group However, the crystal structure of the Phe35fiTyr mutant shows that the ˚ hydroxyl oxygen atom of the side chain of Tyr35 is 3.21 A away from the atom CHB of the pyrrole group of the ... conformation of this residue The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the ˚ wild-type cyt b5 is 0.21 A, within the error limit The side chain of Tyr35 of the...
... Biochem 269) are grouped in a superfamily of metzincin which exhibits some typical structural features, such as the Met-turn and active-site consensus HExxHxxGxxH sequence [15–17] Some organisms and ... carbonyl oxygen of Asn107 (c) The C-terminal carbonyl oxygens of these residues are hydrogen-bonded to the N-terminal nitrogen of Ile109 In addition, the side chain of Lys residue also contacts extensively ... potencies of pEWN and pEWK (Table 3) Structural comparison of pENW-(TM-3) with the peptidic inhibitor-complexes of atrolysin C, TACE and HNC Fig Diagram of the active-site structure of TM-3 complexed...
... Laboratory The X- raywavelength was 0.154 nm with a beam geometry of $ 0.2 · 0.5 mm in a mica sandwich cell with a surface of · mm and a path length of 0.5 mm Simultaneous SAXS and WAXS intensities ... changes observed in the SAXS ⁄ WAXS profiles are consistent with a progressive replacement of a gel phase of brainSM and the disappearance of a small proportion of a coexisting highly disordered ... concluded from analysis of the behaviour of this ternary mixture that the properties of the major constituent of the mixture, namely, long N-acyl chain molecular species of sphingomyelin, tend...
... an rmsd of approxi˚ mately 1.6 A from that of MtPpiA, when the Ca atoms are ˚ compared using a cut-off of 3.5 A (with 88% of the Cas matching) This is significantly larger than the rmsd of ˚ 0.05 ... the presence of a hexa-peptide of sequence HAGPIA derived from the HIV capsid protein sequence [14] The expected site for binding the hexa-peptide is indicated using a superposition of the human ... Number of reflections used in working set Number of reflections for Rfree calculation R, Rfree (%) Number of nonhydrogen atoms Number of solvent waters ˚ Mean B -factor, protein atoms (A2) ˚ Mean B -factor, ...
... the binding of the thiol sulfur of HS-CoM to the zinc atom of MtaA in a way that it replaces one of the nonsulfur ligands, possibly the oxygen of coordinated H2O or hydroxide EXAFS of two MtaA ... EXAFS oscillations of Fig Fourier transforms (FT) of k3-weighted EXAFS spectra of wildtype MtaA and of two MtaA mutants (Cys239 Ala, His237 Ala) in the absence and presence of coenzyme M Experimental ... ligated to the zinc of MtaA increases upon formation of the MtaAcoenzyme M complex explainable by ligation of the thiol sulfur of coenzyme M to the active-site zinc We simulated the experimental spectra...
... values of 17% and 22% (Table 1) Electron density was observed for all except residues 1–2 and 308–309 of the complete sequence in both molecules of the asymmetric unit The structure is composed of ... cut-off of 0.5 A, it is seen that there is a very small (1.5°) difference in their relative orientations A nearly perfect twofold axis (179°) relates the two molecules, ˚ with 750 A2 on domain of ... glycoside oxygen of GGal Three other residues are exclusively linked to binding of the glyceryl moiety (marked with red ovals in Fig 2B): B Fig Interactions in the binding site (A) Stereoview of bound...