... fetal stemcells (5) Adultstemcells might provide medical solutions that avoid the ethical and legal problems of cloning and fetal stem cell approaches Until recently, stemcells from adult ... the tissues to regenerate organs include cloned cells, embryonic or fetal stem cells, or adultstemcells Although each of these sources of stemcells has potential biological advantages and disadvantages, ... emerging on adultstemcells Much of the public attention and debate has been focused on the possibility that adultstemcells may be used as a substitute for human embryonic stemcells or as...
... Muotri AR, Zatz M: Multipotent stemcells from umbilical cord: cord is richer than blood! StemCells 2008, 26(1):146-150 Zhang MJ, Liu B, Xia W, Sun ZY, Lu KH: Could cells from menstrual blood be ... Discussion The possibility of using stemcells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through ... 44:215-230 Phinney D, Prockop D: Mesenchymal stem/ multipotent stromal cells: the state of transdifferentiation and modes of tissue repair – current views StemCells 2007, 25:2896-28902 Valtieri M, Sorrentino...
... neuronal cells in the adult brain originate from residual stemcells The existence of stemcells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells ... endogenous neural progenitor or stemcells and the transplantation of adult- derived neural progenitor and stemcells [39] Self-renewing multipotent neural progenitor and stemcells have been isolated ... the nervous system Adult- derived neural progenitor and stemcells grafted in the brain promote neuroprotection, by an immunomodulatory mechanism Grafted neural progenitor and stemcells interact...
... ‘bone marrow stem cell’, which is often used for either hematopoietic or mesenchymal stem cells, the central nervous system stem cell, the hepatic stem cell, etc.? If many stemcells are found ... regeneration [134] Mesenchymal stemcells versus embryonic stemcells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can be maintained indefinitely ... consensus as to whether all these pools of stemcells are separate entities or whether they are all descendants of one common stem cell Are the true stemcells circulating and homing to tissues...
... myogenesis in adultstemcells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent study showed the highly successful induction of myogenesis from adult ... techniques can be used to label stemcells [79] In other systems, asymmetric division has been shown to be integral to stem cell self-renewal This unique property of stemcells has been exploited to ... Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy J Cell Mol Med 2004, 8:301-316 31 Tuan RS, Boland G, Tuli R: Adult mesenchymal stem cells...
... Stemcells 1.5.1.1 Embryonic stemcells 1.5.1.2 ES cell stem therapy is not ideal for clinical use 1.5.2 Adultstemcells 10 1.5.2.1 Bone marrow stemcells ... cardiovascular research 1.5.2.1 Bone marrow stemcells There are most common types of stemcells in bone marrow: (i) hematopoietic stemcells and (ii) mesenchymal stemcells These cell types are believed ... the traditional dogma of adultstem cell commitment and presents the impetus for a burgeoning interest in adultstem cell therapeutics The use of adult bone marrow stemcells in cellular transplantation...
... Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human StemCells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymal stemcells are capable of ... transplantation of stem cells, it is very important to observe the survival and curative effects of transplanted stemcells Traditional immunohistochemical method can easily identify the transplanted cells ... expressed in injured tissues play important roles (2) Stemcells circulate among tissues, and stemcells migrate to the injured tissues once injury occurs Stem cell homing is a complicated process in...
... efficient and safe xeno-free cryopreservation method for the storage of human embryonic stemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells ... so as to enable some of the cells to undergo mitosis Mitotic hESC were then arrested at metaphase by colcemid treatment (0.1 μg/ml) for 16-18 h Subsequently, the cells were subjected to hypotonic...
... adult human mesenchymal stemcells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem ... mesenchymal stemcells and mononuclear cellsStemCells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal stem ... & Chen X (2008) Lysophosphatidic acid protects mesenchymal stemcells against hypoxia and serum deprivation-induced apoptosis StemCells 26, 135–145 21 Li Z, Wei H, Liu X, Hu S, Cong X & Chen...
... outcome in described stem cell types Stem cell type BMP signal outcome Embryonic stemcells (i) Embryonic stemcells (ii) Hair-cell progenitors (inner ear) Mesodermal progenitor cells Stemness upkeep ... embryonic stemcells J Cell Biol 138, 1207–1217 Sumi T, Fujimoto Y, Nakatsuji N & Suemori H (2004) STAT3 is dispensable for maintenance of self-renewal in nonhuman primate embryonic stemcellsStemCells ... human embryonic stemcellsStemCells 22, 770–778 Ying QL, Nichols J, Chambers I & Smith A (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal...
... introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and adult ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stemcells and in human CD 133+ bone marrow cells ... differentiation of murine embryonic stemcells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stemcells at the indicated time points...
... Section Neural StemCells as Progenitor Cells Chapter Systems for ex-vivo Isolation and Culturing of Neural StemCells Simona Casarosa, Jacopo Zasso and Luciano Conti Chapter Neural Stem Cell Heterogeneity ... of stemcells from the fetal and adult central nervous system Genes Dev 1996;10(24):3129-40 [58] Palmer TD, Takahashi J, Gage FH The adult rat hippocampus contains primordial neural stemcells ... sphereformation as an assay for stemcells Cell stem cell 2011;8(5):486-98 Epub 2011/05/10 [49] Chojnacki AK, Mak GK, Weiss S Identity crisis for adult periventricular neural stem cells: subventricular...
... Shinya Yamanaka StemCells Make Copies of Themselves Through Cell Division, or … StemCells Adapt for Many Parts of the Body StemCells Brain Bone marrow Fat Heart Pancreas Pluripotent Stem Cell Sources ... Embryonic StemCells from Somatic Cell Nuclear Transfer (SCNT) If inner cell mass is placed in culture, the stemcells will divide Develops into a blastocyst Asexual production of pluripotent stemcells ... reproductive cloning Unsuccessful thus far in humans • Induced pluripotent stemcells (iPS) created by transferring about genes into adultcells Dominic Doyle Human Blastocyst 0.1-0.2mm diameter Day 4-5...
... and techniques of stem cell research are compared to that of reproductive cloning Embryonic StemCells Produced with the SCNT Technique Adult and Fetal StemCells Embryonic StemCells Reproductive ... investigators are seeing adultstemcells that truly have plasticity or whether some tissues contain several types of stemcells that each give rise to only a few derivative types Adultstemcells are rare, ... leukemic cells (Saito et al., 2000) OTHER ADULTSTEMCELLS During the past years, scientific reports of stemcells in other organs of adult mice—including brain, muscle, skin, digestive system,...
... reprogramming of adult skin fibroblasts into induced pluripotent stemcells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... and more recently, of human induced pluripotent stemcells The exploration and realization of the incredible potential of these pluripotent stemcells for studying the fundamentals of early human ... Pluripotent StemCells Derivation and Culturing, summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and...
... Placenta StemCells 150 Anthony Atala 15 AdultStemCells in the Human Endometrium 160 ´, ´n Caroline E Gargett, Irene Cervello Sonya Hubbard, and Carlos Simo 16 Stem Cell Populations in Adult Bone ... Thaddeus G Golos 13 Reproductive StemCells of Embryonic Origin: Comparative Properties and Potential Benefits of Human Embryonic StemCells and Wharton’s Jelly StemCells 136 Chui-Yee Fong, Kalamegam ... of adult human mesenchymal stemcells Science 1999; 284:143–147 Goodman JW, Hodgson GS Evidence for stemcells in the peripheral blood of mice Blood 1962; 19:702–714 Gamete Generation from Stem...
... of embryonic stemcells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stemcells derived ... profiles Epigenetic study of embryonic stemcells Differences in DNA methylation profiles between ES and other stemcells Comparing ES cells with other stemcells established from developing embryos ... the ED between ES and EG cells (49) is less than that between ES and TS cells (77), confirming the previous notion that EG cells are more similar to ES cells than to TS cells (Fig 1) Change of...
... mesenchymal stemcells of fetal or maternal origin from human placenta StemCells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stemcells ... differentiation from stemcells For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte-like cells using the original protocol (Fig 1A) The differentiated cells (AT-MSC-Hepa) ... into hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stemcells for...
... (slowly dividing astroglial cells and the bona fide adult neural stem cells) , type C cells (transit-amplifying cells derived from asymmetric division of Type B cells) , type A cells (immature neuroblasts ... glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stemcells but not retinal stemcells Eur J Neurosci, Vol 18, No 1, (July ... orders@intechweb.org Neural StemCells and Therapy, Edited by Tao Sun p cm ISBN 978-953-307-958-5 Contents Preface IX Part Chapter Characterization of Neural StemCells Neural StemCells from Mammalian...