extraction sequence isolation and phylogenetic analyses
Isolation and expression analyses of dazl, vasa and sox3 genes in the seabass (lates calcarifer)
... GenBank database and used for amino acid comparisons and phylogenetic analyses The amino acid sequences were aligned using Vector NTI Suite 7, and the phylogenetic trees were constructed using DNAMAN ... Materials and Methods 2.1 Experimental animals Seabass were from a local fish farmer Fertile male and female were selected for the extraction of testis, ovary and other essential organs for the isolation ... group B2 28 and Sry in group A (Schepers et al., 2002) For Vasa analysis, the lcVasa protein and its homologs were used for phylogenetic comparison and their sub-families PL10, p68 and GLH were...
- 98
- 312
- 0
Báo cáo hóa học: " Complete genome of a European hepatitis C virus subtype 1g isolate: phylogenetic and genetic analyses" pptx
... phylogenetic tree of complete genome sequences Maximum likelihood phylogenetic tree of complete genome sequences Phylogenetic tree was obtained with PHYML using GTR+G+I for the new sequence and ... contributions MAB, VS, and EM co-conceived, designed and coordinated the study, participated in the molecular studies, sequence alignment, phylogenetic and genetic analyses, interpreted data, and co-drafted ... and resided in Spain, which is evidence of local transmission of subtype 1g Conclusion Finally, we carried out phylogenetic analyses with our subtype 1g sequence and all available deposited sequences...
- 7
- 434
- 0
báo cáo khoa học: " Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome" pdf
... 384-well plates and gDNA inserts were Sanger-sequenced from both ends Sequences were scanned and masked for vector sequences and contaminating bacterial sequences, eliminating 21.4% (PGB02) and 27.9% ... automated and manual sequence assemblies resulted in two contigs for PGB02 with a combined sequence length of 172,056 bp and 15.6× sequence coverage, and into a single contigs for PGB04 with a sequence ... for the PGB04 library Sequences were scanned and masked for vector sequences and contaminating bacterial sequences, eliminating 21.4% (PGB02) and 27.9% (PGB04) of the total sequences Using PHRAP,...
- 13
- 329
- 0
Báo cáo y học: " Sequence and phylogenetic analysis of H7N3 avian influenza viruses isolated from poultry in Pakistan 1995-2004" pot
... contributions MAA, ZA, LS and NS produced and analyzed the sequence data ES was involved in experimental design, performed the phylogenetic analysis and sequence analysis DES, KN, AH and SR were involved ... City, CA) GenBank accession numbers are given in Table Phylogenetic and Sequence Analysis Phylogenetic analysis included any available sequence data from H7N3 isolates from Pakistan (therefore ... NS, M, NP, PA and PB1 of 100/Karachi-04 which groups with the H9N2 AIVs from Pakistan, and 2) the HA genes of 34668/Pak-95 and 35/Chakwal-01 which group with A/Parrot/NorthIreland/VF-73-67/73...
- 10
- 255
- 0
Báo cáo y học: " Phylogenetic analyses of the polyprotein coding sequences of serotype O foot-and-mouth disease viruses in East Africa: evidence for interserotypic recombination" pps
... in this study include isolates from Kenya and Uganda obtained between the years 1992 and 2006 and sequences already in the Genbank database Materials and methods Viral isolates The viruses investigated ... further analyses were performed using similarity and bootscan plots (see Figure and 7) The similarity and bootscan plots show the pairwise genetic similarities and bootscan values between a query sequence ... burnin [23] Recombination between sequences was analysed using SimPlot method 2.5 software [24] Results Sequence Characteristics and Phylogenetic Relationships Sequence Analysis Sequencher software...
- 9
- 363
- 0
Automatic text extraction using DWT and Neural Network
... video sequences using DWT and neural network DWT decomposes one original image into four sub-bands The transformed image includes one average component sub-band and three detail component sub-bands ... the proposed method Finally, we conclude in section Feature extraction Candidate text extraction using Neural Network Text regions extraction results Figure Flow chart of the proposed algorithm ... operation and the final resulted 2-D Haar DWT is shown in Figure 3(c) 2-D Haar DWT decomposes a gray-level image into one average component sub-band and three detail component sub-bands From...
- 5
- 507
- 1
Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...
- 12
- 510
- 1
Exergoeconomic performance optimization of an endoreversible intercooled regenerated Brayton cogeneration plant Part 1: Thermodynamic model and parameter analyses
... thermal capacity rate (mass flow rate and specific heat product) Cwf The hot-, cold- and consumer-side heat reservoir temperatures are TH , TL and TK respectively, and the intercooling fluid temperature ... fluid and the heat reservoirs, the regenerator and the intercooler are counter-flow The conductances (heat transfer surface area and heat transfer coefficient product) of the hot-, cold- and consumer-side ... decreases, and π Π max increases first, and then decreases, but the value of π Π max changes slightly Figure Characteristics of Π max and (ηex )Π versus τ max Figure 10 Characteristics of π Π max and...
- 12
- 495
- 0
Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... interactions: binding and cleavage by ribonuclease A and variants at Lys7, Arg10, and Lys66 Biochemistry 37, 1212112132 31 Anderson DG, Hammes GG & Walz FG Jr (1968) Binding of phosphate ligands to ribonuclease ... RiIi(h), where Ii(h) and I(h) are the ith and the mean measurements of the intensity of reection h, respectively Rcryst = Rh |Fo ) Fc| RhFo, where Fo and Fc are the observed and calculated structure ... dashed lines, and water molecules are in cyan (C, D) Stereo pictures of 2Fo ) Fc contoured at 1.0r for N-acylsulfonamide and N-acylsulfonamide 6, respectively N-Acylsulfonamide (2Â-deoxy) and N-acylsulfonamide...
- 9
- 626
- 0
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... hydrolyze d,l-p-HPH SDS–PAGE analyses of wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf ... HYDBp and HYDJs tot, total proteins; ppt, precipitate fraction; sup, supernatant fraction; puri, purified proteins; M, molecular weight standards For the molecular weight standards, the bands from ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared...
- 14
- 621
- 0
Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS Half maximal inhibitory concentration (IC50) and median lethal dose (LD50) values from HeLa cells and mice divided ... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... acid sequence analyses and Dr Robert Spooner for help with cytotoxicity assays References 13 14 Girbes T, Ferreras JM, Arias FJ & Stirpe F (2004) Description, distribution, activity and phylogenetic...
- 12
- 763
- 0
Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... understanding of genetic mechanisms of endothelial development and functions However, further analyses of vascular phenotypes in vivo have been hampered by the complicated relationship between ECs and ... these cells Mesenteric BECs and LECs (Fig 5A,B) were also obtained by the same strategy as illustrated in Figs and 4, and were treated with VEGF-A or VEGF-C (Fig 5C) MAPK and Akt phosphorylation ... induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolation and characterization of BECs and LECs We next...
- 11
- 873
- 0
Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... of the c-proteobacteria A vinelandii and P luminescens and to Idi-1 protein of the Bacteroid C hutchinsonii (expect values 3e-22, 5e-19 and 8e-20, respectively) Phylogenetic pattern of Idi proteins ... (1996) SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny Comput Applic Biosci 12, 543–548 39 Dayhoff, M.O (1979) Atlas of Protein Sequence and Structure Vol5 ... algorithms [36] Nucleic acid sequences of unfinished genomes were downloaded from the GenBank database, and open reading frames were identified and translated into amino acid sequences with the program...
- 12
- 692
- 0
Tài liệu Báo cáo khoa học: "Cross-Domain Co-Extraction of Sentiment and Topic Lexicons" pdf
... classifiers fO and fP for l sentiment- and topic- word extraction with DS DT T separately Predict the sentiment score hfO (wTj ) and u topic score hTP (wTj ) on DT , and select k2 sentiment f words and ... words with highest scores as candidates 5: Construct a bipartite graph between sentiment and topic l words on DT and the k2 sentiment- and topic- word candidates, and calculate the normalized ... the scores S1 and S3 of the k2 sentiment and topic word candidates using Eqs (5) and (6) iteratively 7: Select k1 new sentiment words and k1 new topic words with the final scores, and add them...
- 10
- 447
- 0
Tài liệu Báo cáo khoa học: "Toward finer-grained sentiment identification in product reviews through linguistic and ontological analyses" ppt
... ‘thickness’ Hu and Liu (2004) identified attributes of IT products based on frequent noun phrases and Popescu and Etzioni (2005) utilized PMI values between product class (hotels and scanners) and some ... quality element classification (Huiskonen and Pirttila, 1998), the conceptual granularities, and such syntactic and lexical clues as conjunction items and adverbs They may play significant roles ... significant differences between ‘likeness’ and ‘contrary’ (p = 015), and between ‘likeness’ and ‘contrary with contrastive markers’ (p = 025) The ‘contrary’ and ‘contrary with contrastive markers’...
- 4
- 337
- 0
Tài liệu Báo cáo khoa học: "Answer Extraction, Semantic Clustering, and Extractive Summarization for Clinical Question Answering" pdf
... conciseness and completeness by providing hierarchical and inter842 UMLS concepts UMLS has an extensive coverage of drugs, falling under the semantic type P HARMACOLOGICAL S UBSTANCE and a few ... between system output and drugs mentioned in CE, primarily due to synonymy (for example, alpha-adrenergic blocking agent and αblocker refer to the same class of drugs) and ontological mismatch ... “okay” (marginal), or “bad” based both on CE and her own experience, taking into account the abstract title and the topscoring outcome sentence (and if necessary, the entire abstract text) Results...
- 8
- 372
- 0
Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and averaged ... NH2-terminal sequence PHI purification After SDS/PAGE, the protein band was blotted onto Immobilon P (Millipore) membrane The N-terminus was sequenced using the Applied Biosystems 470A automatic microsequencer,...
- 7
- 514
- 0
Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... (Fig 1A, lanes and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained ... Results and discussion Electrophoretic profile Figure 1A shows the SDS/PAGE of purified ccNiR upon different treatments The complex dissociates into an intense band of 61 kDa (NrfA) and a band of ... column, equilibrated and eluted in 0.1 M Tris/HCl, pH 7.6 and several common protein–protein dissociation salts (1 M sodium chloride, M urea and M guanidinium chloride) and ionic detergents (CHAPS,...
- 12
- 593
- 0
Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf
... PR, and the respective S4 and S3 binding sites could accept a variety of residues [33,34,46] When compared to peptides having the same natural sequence background (peptides and 1, and 2, and ... frequently occurring mutations found as sequence polymorphisms and in drug resistance, with wild-type and five drug-resistant mutant PRs: M46L, V82A, I84V and L90M mutations that appeared together ... peptides representing NC/p1 cleavage site sequences by wild-type and mutant HIV-1 proteinases Oligopeptides including both natural sequence polymorphisms and resistant mutations of the NC/p1 cleavage...
- 7
- 480
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25