extraction reverse transcription and real time quantitative polymerase chain reaction
Báo cáo y học: "Cost and mortality prediction using polymerase chain reaction pathogen detection in sepsis: evidence from three observational trials" pot
... Falagas ME, Tassios PT: Enhanced and earlier detection of bacteremia and fungemia by multiplex polymerase chain reaction: how much enhanced, how much earlier, and at what cost? Crit Care Med 2008, ... hematological malignancies J Infect Dis 2006, 193:741-747 15 Jordan JA, Durso MB: Real- time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated ... Rapid identification and differentiation of Gram-negative and Gram-positive bacterial bloodstream infections by quantitative polymerase chain reaction in preterm infants Crit Care Med 2009, 37:2441-2447...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... Society [32] SD, standard deviation Table Sequences of the gene-specific oligonucleotide primers and probes for real- time reverse transcriptase polymerase chain reaction Primer and probe HAS-1 Sequence ... (OA) and rheumatoid arthritis (RA) Total RNA was isolated from knee synovium and expression levels of the messages were relatively quantified by real- time reverse transcriptase polymerase chain reaction ... -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid arthritis for hyaluronanof knees in 2, and -3 and hyaluronidase-1, -2, and...
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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
... Analysis by Real- Time Quantitative PCR Quantitative PCR was performed using the Sequence Detector System, ABI PRISM 7500 (Applied Biosystems 7500 Fast Real- Time PCR System) PCR primers and the TaqMan ... Yasui K, Kato T, Tatematsu M: Quantitative detection of disseminated free cancer cells in peritoneal washes with real- time reverse transcriptasepolymerase chain reaction: a sensitive predictor ... Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real- time reverse transcriptase -polymerase chain reaction to predict recurrence of gastric adenocarcinoma Journal...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... remainder of the reactions were made up by µl PCR Buffer (Gibco BRL, Grand Island, NY), 0.25 µg of each primer, mM MgC12 in each CD4 reaction and mM MgC12 for CD8 reactions, mM total dNTP's, and 0.5 U ... blotting or real- time PCR, would reveal a correlation either Conclusions QC-RT-PCR and related techniques such as real- time PCR are not useful as substitutes for assessment of CD4 and CD8 cell ... amounts of blood, such as that from a heelstick or finger-prick [2] Quantitative methods for reverse transcriptase polymerase chain reaction (RT-PCR) are rapidly surpassing all other methods of quantifying...
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Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
... used for RNA extraction and duplex real- time RT-PCR analysis Table demonstrates that the duplex real- time RT-PCR assay was 10-fold more sensitive than virus isolation for both WEEV and EEEV detection ... validated a duplex realtime RT-PCR system for both WEEV and EEEV detection at the same time The primers and probes were selected from E3 gene for WEEV and NSP3 gene for EEEV, and the sequence analysis ... Kramer LD, Bernard KA, Tavakoli NP: A duplex realtime reverse transcriptase polymerase chain reaction assay for the detection of St Louis encephalitis and eastern equine encephalitis viruses Diagn...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
... driven cytokine production, antigen specific cytotoxicity and lymphoproliferation assays or quantitative real- time polymerase chain reaction (qRT-PCR) for the detection of cytokine gene expression ... peripheral blood mononuclear cells and tumor specimens J Immunol 1999, 163:6867-6875 Kammula US, Marincola FM, Rosenberg SA: Real- time quantitative polymerase chain reaction assessment of immune reactivity ... Paisley, UK) and incubated at 37°C for hour Two μl of cDNA were used for each PCR amplification by "real time" technology (7300 Real Time PCR system, Applied Biosystems, Rotkreuz, Switzerland) according...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat ehT )ASU ,ratsanD( erawtfos ratsanD eht gnisu dengila dna )1 elbaT( A C aisA 2TAS O O O O O O O O 55/lizarB/oriezurC/42A ... eht gnitarapes ybereht ,esaremylop AND qaT fo ytivitca esaelcunoxe eht ot eud dedarged si eborp eht ,srucco noitacifilpma sA tegrat RCP eht nihtiw ecneuqes AND dnarts-elgnis yratnemelpmoc sti...
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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
... transplantation by real- time PCR of Page of which two were positive by pp65 antigenemia assay and 12 were negative by both real- time PCR and pp65 antigenemia assay One donor was positive only by realtime PCR ... antigenemia and real - time PCR The highest copy number detected by real - time PCR was 29, 768 copies/ml and lowest was copies/ml The highest positive pp65 cell count recorded among them was 78 cells and ... Madhavan et al.: “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up...
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báo cáo khoa học: " Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data" potx
... Inactivation of the reverse transcriptase was done by incubating the mixture at 70°C for 15 and the cDNA solution was stored at -20°C Page of 12 Real- time quantitative polymerase chain reaction (qPCR) ... of gene expression and internal standard selection Biotechnology Advances 2009, 27(4):323-333 12 Andersen CLJJ, Orntoft TF: Normalization of real- time quantitative reverse transcription- PCR data: ... identification of genes relevant to new biological processes [1] Reverse transcription real- time quantitative polymerase chain reaction (qPCR) is a robust method to study gene expression changes [2]...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... RT-PCR and nested PCR in the diagnosis of canine distemper Vet Res Commun 2005, 29:347-59 10 Li JZ, He HB, Xia XZ, et al.: Establishment and application of reverse transcription Polymerase chain reaction ... Cite this article as: Si et al., A multiplex reverse transcription- nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus ... confirmed by Rapid test which BioNote, Inc produced Multiplex reverse transcription- nested polymerase chain reaction Phylogenetic analysis and detection of CDV in field samples by RT-nPCR Total RNA...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... used realtime polymerase chain reaction- based method Hepatology 2007, 46:22-31 Chevaliez S, Bouvier-Alias M, Castéra L, Pawlotsky JM: The Cobas AmpliPrep-Cobas TaqMan real- time polymerase chain reaction ... HD, Grant PR, Garson JA, Tedder RS, Chandy GM, Abraham P: Quantitation of hepatitis C virus using an in-house real- time reverse transcriptase polymerase chain reaction in plasma samples Diagn Microbiol ... between two real- time PCR-based hepatitis C virus (HCV) assays (RealTime HCV and Cobas AmpliPrep/Cobas TaqMan) and one signal amplification assay (Versant HCV RNA 3.0) for RNA detection and quantification...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
... the fluorescent quantitative real- time PCR (FQ-PCR) standard curve Establishment of the fluorescent quantitative real- time PCR (FQ-PCR) standard curve Ten-fold dilutions of standard DNA ranging ... Knowles NJ, Hutchings GH, Cooper EJ, Smith AW, Ferris NP: Development of a real- time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus Vesivirus) ... postinoculation (PI) time points, at 30 min; 1, 2, 4, 8, 12, and 24 h; and 2, 3, 6, and days Tissues were surgically removed from the goslings and frozen at -80°C, weighed, and homogenized using...
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Báo cáo y học: "Base relative quantification framework and software for management and automated analysis of real-time quantitative PCR data" potx
... 28.95 Volume 8, Issue 2, Article R19 comment Sample Standard1 Standard1 Standard2 Standard2 Standard3 Standard3 Standard4 Standard4 Standard5 Standard5 Genome Biology 2007, R19.4 Genome Biology 2007, ... part, in Table 18 time PCR BMC Bioinformatics 2006, 7:107 Nordgard O, Kvaloy JT, Farmen RK, Heikkila R: Error propagation in relative real- time reverse transcription polymerase chain reaction quantification ... transformed ratio and the standard deviation Virc for all runs l and a given IRC combination (m, m') and a given gene j Vsample and Vrun are calculated similarly from Asample and Arun, respectively:...
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3D Videocommunication Algorithms concepts and real time systems in human centred communication
... analysis of audio and video and arbitrary view and sound synthesis to encoding of 3D audio and video Understanding of real- time implementation issues, as well as system architectures and network aspects ... 11 Coding and Standardization by A Smolic and T Sikora outlines the basic coding strategies frequently used for audio, image and video storage and transmission International coding standards such ... the internet via a camera and where remote users could control a robotic arm to plant and water seeds, and subsequently watch their plants grow and flourish in real- time 1.6 TELECOMMUNICATIONS...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... (H5N1) viruses and detection of anti-H5 antibody among household and social contacts”, J Infect Dis, 180, pp 1763-70 36 Lamb RA and Krug RM (2001), “Orthomyxoviridae: The Viruses and Their Replication”, ... fixation and hemagglutination inhibition tests in the diagnosis of influenza A and B virus infections”, Purified hemagglutinin in subtype-specific diagnosis, J Vir.Methods 10, 75-84 34 Kandun IN, ... H5N1 influenza virus in Asia: implications for pandemic control”, Proc Natl Acad Sci USA, 103, pp 2845- 50 15 Chen, H., G Deng, Z Li, G Tian, Y Li, and P Jiao (2004), “The evolution of H5N1 influenza...
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Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
... virus isolation and real- time RT-PCR -/-: Negative results in both virus isolation and real- time RT-PCR 350 Dong Kun Yang et al has several advantages over conventional PCR First, realtime RT-PCR ... TCID50 /reaction to 2.24 × 101.0 TCID50 /reaction Real- time RT-PCR assay could detect JE RNA ranging from 1.12 × 106.0 to 1.12 × 101.0 TCID50/ml Therefore, detection limits of RT-PCR and real- time ... DNA polymerase and to deactivate reverse transcriptase; 45 or 50 cycles of 10 s at 95oC to denature and 20 s at 55oC to anneal and 20 s at 72oC for extension and at 72oC for final extension Reverse...
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Báo cáo y học: "Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reacti" potx
... practical and powerful approach to multiple testing J Roy Statist Soc Ser B 1995, 57:289-300 Bustin SA, Nolan T: Pitfalls of quantitative real- time reversetranscription polymerase chain reaction ... RNAse-free water, and reverse transcription was performed using 10 μl RNA (200 μg total RNA) with oligo-dT12– 18 and Superscript II reverse transcriptase (Invitrogen Ltd) After reverse transcription, ... median values and MannWhitney U tests because the data were not normally distributed Table The dynamic range, standard curve slope, R2 value, and efficiency of each polymerase chain reaction assay...
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Báo cáo y học: "Mechanical effects of surgical procedures on osteochondral grafts elucidated by osmotic loading and real-time ultrasound" ppsx
... oversized and exact-sized cartilage plugs in osteochondral grafting using osmotic loading and real- time ultrasound The main findings of the study are that osmotic loading and real- time ultrasound ... and real- time ultrasound may contribute to tissue engineering in the musculoskeletal field, and the ARR and echo shifts can be expected to become quantitative indices for the biomechanical and ... and real- time ultrasound Under osmotic loading, the changes in the amplitude and echo shifts can support the evaluation of cartilage damage in osteochondral grafts Moreover, osmotic loading and...
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báo cáo khoa học: " Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR" pps
... 1.5 ul 10× reaction buffer, 0.19 μl RNase inhibitor, μl Reverse transcriptase, and μl gene-specific primer in a 15-μl reaction The real- time PCR for each assay was set up as a 20 μl reaction including ... μl of each primers (900 nM), μl Taqman probe (250 nM), and 1.5 μl cDNA sample All real- time PCR reactions were performed in a StepOne real- time PCR machine (Applied Biosystems, Foster City, CA) ... (miR159a and miR159b) have been sequenced more than 5,000 times in mature pollen Since the expression of these two miRNAs was not detected by either real- time PCR or regular RT-PCR (Table and Figure...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... single-tube real- time reverse transcription- PCR assay J Clin Microbiol 2001, 39:4093-4096 Mohamed N, Elfaitouri A, Fohlman J, Friman G, Blomberg J: A sensitive and quantitative single-tube real- time reverse ... assays, RNA extraction and realtime PCR analysis NJ drafted and wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed ... cellular receptor J Virol 2000, 74:42-48 Jonsson N, Gullberg M, Lindberg AM: Real- time polymerase chain reaction as a rapid and efficient alternative to estimation of picornavirus titers by tissue...
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