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extraction isolation purification and analysis

Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Cao đẳng - Đại học

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that ... confounding authentic saponin identification and quantification The purification process is complex and laborious with long sample preparation and extraction times and yielding relatively low amounts...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

Báo cáo khoa học

... GLUT1 and GLUT3 (exons 3–8), and four in human GLUT2 (exons 7–10) and GLUT4 (exons 6–9) (Table 1) The codons for arginine (96) and valine (231) in gcGLUT (Fig 2) are split between exons and 5, and ... PstI and XbaI, and southern hybridization analysis using GT-cDNA2 as a probe Northern blot analysis Total RNA (20 lg) from different tissues was electrophoresed on 1% agarose/formaldehyde gels and ... (between exon and exon 5) and valine (between exons and exon 7) are shown Exonic regions are shown in uppercase and intronic regions are in lowercase The split codons are boxed and highlighted...
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oil extraction and analysis phần 1 ppsx

oil extraction and analysis phần 1 ppsx

Tự động hóa

... industry and the regulations of the European Commission Fats and oils are handled by TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products—Methods of sampling and analysis. ” ... SDOs Fats, Oils, and Lipids Methods Standardization There is a long list of developers of national and international standards Although national standards bodies generally adopt standards methods ... of oilseeds generally go hand in hand Reviews of the production of oilseed and oils and fats are regularly produced by world experts Frank Gunstone and Thomas Mielke and may be found in the AOCS...
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oil extraction and analysis phần 2 pps

oil extraction and analysis phần 2 pps

Tự động hóa

... detergents Extraction of paraffin from detergent Extractable matter in leather Extraction of petroleum source rock Extraction of explosives and propellants Extraction of plastics and polymers Extraction ... flexibility and improved economy as well as enhancement of the precision and recovery of the extraction References Randall, E.L (1974) Improved Method for Fat and Oil Analysis by a New Process of Extraction, ... of rubber and rubber compounds Extraction of finish oils from textiles and synthetic fibers Extraction of migration components in plastic packaging Extraction of organic dyestuffs Extraction...
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oil extraction and analysis phần 3 docx

oil extraction and analysis phần 3 docx

Tự động hóa

... solvent extraction (ASE), also referred to as pressurized fluid extraction (PFE) and pressurized liquid extraction (PLE), is a liquid solvent extraction technique that uses aqueous and organic extraction ... material Sample Extraction Parameters Extraction Solvent As extraction parameters, solvent choice and temperature have the greatest effect on extraction efficiency with ASE An extraction solvent ... the analysis in F1 and F2 stages of plant breeding and for screening rare and elite germplasm lines in which sample amounts available are always limited Extraction of Tocopherols from Soy and...
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oil extraction and analysis phần 4 doc

oil extraction and analysis phần 4 doc

Tự động hóa

... Mike Anson, and Andrew Ormond, Tyson Foods/IBP, Dakota City, NE and Waterloo, IA; Linda Wesolowski and Teri Crockett, Agribrands Purina Canada, Strathroy, ON, Canada; Bruce George and Andrew Ormond, ... sample handling and sample homogeneity FBT Extraction Temperature Because elevated solvent temperatures enhance the extraction kinetics, the effects of extractions at three temperatures, 85, 90, and ... the same solvent and the extraction conditions (time and temperature) are sufficient to complete the extraction The extraction is a function of the characteristics of the solvent and the solute...
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oil extraction and analysis phần 5 pps

oil extraction and analysis phần 5 pps

Tự động hóa

... challenges in the techniques of extraction and analysis Conventional methods for isolation of fat-soluble vitamins depend on the sample matrix and usually involve solvent extraction with organic solvents ... Vs, and Vm are the characteristic molar volumes for the binary mixture, principal solvent, and modifying solvent, respectively; xs and xm are the mole fractions for the solvent and modifier; and ... xmωm00 Mwb = xsMws + xmMwm where ωs and ωm are the acentric factors for the solvent and modifier, and Mws and Mwm are the molecular weights of the solvent and modifier The critical pressure Pcb...
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oil extraction and analysis phần 6 potx

oil extraction and analysis phần 6 potx

Tự động hóa

... and 18:1(n-9) Acids from Canola Hull, Meat, and Seed at Each Step of the FOSFA Extraction Relative composition total fatty acid profile 18:1(n-9) 18:1(n-7) Sample Hull Extraction Extraction Extraction ... 18:1(n-9) 18:1(n-7) Sample Hull Extraction Extraction Extraction Meat Extraction Extraction Extraction Seed Extraction Extraction Extraction Relative composition in 18:1 18:1(n-9) 18:1(n-7) (%) 22.5 ... and FFA The relative contents were 98.0, 1.1, 0.0, and 0.9% for TAG, MAG, DAG, and FFA, respectively, for the first extract and 95.6, 1.8, 1.1, and 1.5%, respectively, for the pooled second and...
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oil extraction and analysis phần 7 ppt

oil extraction and analysis phần 7 ppt

Tự động hóa

... extractor (ASE; and g), Ankom (500 mg), and SFE (2 g)] Extraction conditions and sample processing for two sample sizes (100 mg and 1–2 g samples) were the same for all methods The five extraction ... cell bodies and are hand-tightened Inside each cap is a stainless steel frit and a PEEK seal For extraction of oil from corn and soy, 11-mL cells are used Glass vials are used for rinse and collection ... with the glass wool and record the weight (T) Load the extraction cell and collection vial The extraction parameters for corn and soybeans are given in Table 7.2 After the extraction is completed,...
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oil extraction and analysis phần 8 docx

oil extraction and analysis phần 8 docx

Tự động hóa

... methodology for analysis of a food sample in general and then discuss the specifics of meat and dairy samples This new technology for fat analysis has generated a great deal of interest and there is ... products and NIST standards were performed in laboratories at Texas A&M (TAM) and CEM Corporation The results for beef, all beef hot dogs, and chicken are presented in Tables 8.1, 8.2, and 8.3, ... Review Study: The Rapid Determination of Moisture and Fat in Meats and Dairy Products by Microwave Drying and NMR Analysis, Official Methods of Analysis of AOAC International Updates, AOAC International,...
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oil extraction and analysis phần 9 pps

oil extraction and analysis phần 9 pps

Tự động hóa

... America and South America, the official oil content in canola and sunflower is determined by the NMR method All other oilseeds are amenable to the method, including soy, flax, and corn, cotton, and ... 3-94: 1999, 2000 Oil and Moisture; ISO 10565 Oil and Moisture: AOCS Recommended Practice Ak 4-95 Oil: USDA GIPSA Certificate No FGIS00-101 Oil and Moisture: ISO 10632 Oil and Moisture: AOCS Ak ... very fast, compared with oven drying to determine moisture and extraction to determine oil, rapid and more frequent testing is possible, and dry-weight oil is easily reported TD-NMR measures oil...
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oil extraction and analysis phần 10 pot

oil extraction and analysis phần 10 pot

Tự động hóa

... NIR-related research and applications have been reported for oilseed analysis NIR is a rapid, nondestructive, inexpensive, and accurate method for the analysis of traits and material characteristics ... as tofu, miso, and natto Intact soybean analysis for fat, protein, and moisture is preferred (5) The importance of nondestructive analysis is that it can save considerable labor and time The health ... acid and fatty acid composition will benefit the breeding process and commercial soybean testing Pazdernik (6) compared the whole seed and ground soybean NIR analysis for 17 amino acids and fatty...
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oil extraction and analysis phần 11 potx

oil extraction and analysis phần 11 potx

Tự động hóa

... inexpensive, and reliable soybean compositional analysis Certain areas of breeding and selection research would also benefit from single soybean seed analysis (1) Conventional compositional analysis ... mapping and other selection and breeding studies In addition to problems such as low speed and high cost, wet-chemistry methods are destructive and rather inaccurate for single-seed analysis, ... that are designed and optimized only for the major component analysis of bulk grain and oilseed samples For the analysis of minor components such as isoflavones, more flexible and powerful NIR...
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oil extraction and analysis phần 12 pptx

oil extraction and analysis phần 12 pptx

Tự động hóa

... oil, sugars, and/ or moisture The molecular bond’s stretching/vibrations, bending and/ or rotations cause specific absorption peaks or bands, centered at certain characteristic IR and NIR wavelengths ... selected band-pass filters, and by a confocal pinhole in the image space (Fig 12.1) The fluorescence light is then detected, and the corresponding signal autocorrelation is digitized and recorded ... investigation (Figs 12.2 and 12.3) There are four major fluorescence techniques that are currently employed for the analysis and monitoring of molecular interactions and dynamics: fluorescence...
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báo cáo khoa học:

báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc

Báo cáo khoa học

... [33,34], and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding ... suppression subtractive hybridization and microarray analysis J Exp Bot 2007, 58:507-520 Sahi C, Agarwal M, Reddy MK, Sopory SK, Grover A: Isolation and expression analysis of salt stress-associated ... enriched population of the overexpressed and newly induced transcript messages, TA1 and T2R were considered as 'Tester-A' and “Tester-B', respectively, and the C as the 'Driver' The opposite was...
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Báo cáo y học:

Báo cáo y học: "Genome-wide promoter extraction and analysis in human, mouse, and ra" potx

Báo cáo khoa học

... CpG-islands (see Materials and methods), we found that most RefSeq genes are CpG-island related In total, 68%, 54% and 56% promoters obtained above for human, mouse and rat are CpG-island related ... similarity analysis of Ensembl transcripts and genes We obtained 19,179 human-mouse-rat three-species orthologous gene triplets, and 1,967, 1,420 and 2,268 human-mouse, human-rat and mouse-rat ... mouse and rat genes and orthologous gene sets By aligning all known and predicted transcripts to the latest human, mouse and rat genomes we obtained 34,949, 35,073, 30,679 genes (see Materials and...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene

studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene

Tiến sĩ

... DiMario, and Anne Grove for guiding me with their expertise, always welcoming attitude and providing access to their lab equipment and reagents I would like to thank Dr Raymond Schneider and Dr ... REVIEW……………………………………………………1 PARTIAL PURIFICATION OF A CHITINASE-CONTAINING PROTEIN COMPLEX IN THE STRAWBERRY PLANT……………………………32 ISOLATION OF AN OSMOTIN-LIKE PROTEIN GENE FROM STRAWBERRY AND ANALYSIS OF THE RESPONSE ... against Cercospora beticola On the other hand, PR-S failed to inhibit the growth of Trichoderma viride, Candida albicans, and N crassa, while both zeamatin and osmotin completely inhibited the growth...
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Automatic text extraction using DWT and Neural Network

Automatic text extraction using DWT and Neural Network

Kỹ thuật lập trình

... sequences using DWT and neural network DWT decomposes one original image into four sub-bands The transformed image includes one average component sub-band and three detail component sub-bands Each detail ... the proposed method Finally, we conclude in section Feature extraction Candidate text extraction using Neural Network Text regions extraction results Figure Flow chart of the proposed algorithm ... operation and the final resulted 2-D Haar DWT is shown in Figure 3(c) 2-D Haar DWT decomposes a gray-level image into one average component sub-band and three detail component sub-bands From...
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Review and analysis of renewable energy perspectives in Serbia

Review and analysis of renewable energy perspectives in Serbia

Sinh học

... energy sector, its electricity and heat transmission and distribution characteristics and its energy production and consumption features Section provides a detailed analysis of the RES potential ... Energy production and consumption Serbia has a diverse energy supply sector, composed of coal extraction, oil and gas production, imports of crude oil, oil products and gas, coal and hydro electricity ... final electricity and heat consumption, for the years 2004 -2006 and the electricity generation structure and gross consumption for 2009 are presented in Table and Figures and respectively Table...
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E-Recruiting Categories and Analysis of Fortune 100 Career Web Sites

E-Recruiting Categories and Analysis of Fortune 100 Career Web Sites

Anh văn thương mại

... is prohibited 90 Lee and off-line interview and test tools, and then the company conducts an online pre-employment background check and makes a job offer to the best candidate Categories of ... preference, salary, education, and any combination of keywords to find qualified candidates To address job seekers’ and recruiters’ rising dissatisfaction with services and costs, the general-purpose ... Wall Street Journal brand CareerJournal.com provides recruiters and job seekers with a database of job openings and résumés, as well as salary information, career news, and industry trends The...
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