extracellular matrix and tissue regeneration
Báo cáo y học: "Endothelial progenitor cell biology in disease and tissue regeneration" doc
... generated by ischemic tissue and tumor cells including GM-CSF and VEGF, which play a critical role in mobilization of EPCs to ischemic tissues and tumors Hypoxia in tumors and ischemic tissues mediate ... VEGFR1 and VEGFR2 receptors present on endothelial and hematopoietic stem cells and activates matrix metalloproteinase - (MMP-9) which in turn cleaves and activates Kit ligand (KitL) and induces ... CCL2 and CCL5 which help in EPC mobilization [17] EPCs are then released into circulation by activation of MMP-9 which degrades the extracellular matrix and transforms membrane-crossing Kit ligand...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... PuraMatrix forms a relatively soft fibrous matrix, and cannot withstand vacuum aspiration around the hydrogel during medium removal Thus, PuraMatrix is more fragile than Matrigel in terms of handling, ... Available Matrix Substrates Besides Matrigel, other matrix substrates commercially available for hESC proliferation include Geltrex (Gibco), Cultrex (R&D Systems), ECM gel (Sigma-Aldrich) and PuraMatrix ... tool for tissue engineering, cellular transplant therapy and as drug discovery tools [2, 3, 4, 7, 13, 14] In tissue engineering, stem cells can possibly be used to generate healthy tissue to...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... analysis of DxSDOC and DxSDOCDOC matrices Mass spectrometry analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, ... of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098 IPI00009865 58811 IPI00220327 Extracellular Matrix Ribosome-binding protein 66022 IPI00297646 138923 IPI00304962 129297 IPI00013079 IPI00328113 ... 100.00% 10.50% 99.80% 4.61% 100.00% 99.80% 8.29% 8.79% Thrombospondin-1* 100.00% 6.50% A disintegrin and 100.00% 4.96% 343645 48 Membrane Nucleus IPI00018219, IPI00873923, IPI00940091 IPI00022143,...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... aid in increasing integrin binding and hence cell binding This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering ... matrices, despite the relatively high amounts of proteins within and their complexity (refer to section 3.3), not allow the adherence of hESCs and also not maintain hESCs in a pluripotent state This observation ... to FcNP40/DNase matrices either, and also settled on the bottom of the culture well in clusters that were easily moved (Figure 4B) Taken together, DxSNP40/DNase and FcNP40/DNase matrices, despite...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... On the other hand, a much higher percentage for the surface markers SSEA-3 and SSEA-4 was seen 86.9% of hESCs on Matrigel expressed SSEA-3, with 88.9% and 88.7% for hESCs on DxSDOC and DxSDOCDOC ... remained round and tight with defined colony margins As reported earlier, the population doublings of the hESCs on bioassembled matrices DxSDOC and DxSDOCDOC exceeded the control hESCs, and yet, despite ... Matrigel expressed SSEA-4, with 84.2% and 88.1% for DxSDOC and DxSDOCDOC hESCs respectively Only 34.6% of hESCs cultured on Matrigel, 36.8% of hESCs cultured on DxSDOC and 37.2% of hESCs cultured on...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... strong in both samples of hESCs on Matrigel and DxSDOCDOC, at 93.6% TRA-1-60 levels were both high in both samples on Matrigel and 78 DxSDOCDOC, at 73.3% and 77.1% respectively 84.8% of hESCs on ... their high nuclear to cytoplasm ratio, but there were more pockets and areas consisting of differentiated cells with flatter and larger morphologies (arrows) 3.6.4 Pluripotency markers levels ... markers levels by flow cytometry At the 5th passage, hESCs cultured on DxSDOCDOC and on Matrigel were harvested and analyzed by flow cytometry in a similar fashion to that of section 3.5.4 The...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... Hohenester E, Engel J Domain structure and organisation in extracellular matrix proteins Matrix Biol 21(2):115-128 (2002) 63 Schönherr E, Hausser HJ Extracellular matrix and cytokines: a functional unit ... drive extracellular matrix enhancement for the creation of connective tissue in vitro, Tissue Engineering, Croatia: IN-TECH (2010) 44 Minton AP The influence of macromolecular crowding and macromolecular ... application in tissue engineering, stem cell transplant therapy and also drug discovery, hESCs have to be maintained according to GMP standards, which require xeno-free, reproducible and scalable...
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nanotechnology and nanomaterials promises for improved tissue regeneration
... and fibroblast adhesion, proliferation and differentiation Moreover, cartilage is a low regenerative tissue composed of a small Nanotechnology and nanomaterials: Promises for improved tissue regeneration ... (such as fibronectin, vitronectin and laminin) adsorption and bioactivity before cells adhere on implants, further regulating cell behavior and dictating tissue regeneration [12] Furthermore, an ... repair Because the Nanotechnology and nanomaterials: Promises for improved tissue regeneration 71 Figure Self-assembled nanofibers and nanotubes for bone/cartilage tissue engineering applications...
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Báo cáo khoa học: Viral entry mechanisms: human papillomavirus and a long journey from extracellular matrix to the nucleus docx
... units of uronic acid and glucosamine, which are sulfated and acetylated to various degrees O-sulfation occurs at the 2-O, 3-O, and 6-O position of the uronic acid and at the 3-O and 6-O position ... clathrin- and caveolaeindependent internalization of HPV16 pseudovirions in HeLa and HEK 293TT cells [60] Entry and infection was resistant to combined siRNA-mediated down-regulation of caveolin-1 and ... assembly forming a T = icosahedral lattice (Fig 1A) Twelve and sixty capsomeres are pentavalent and hexavalent, respectively (i.e they have five and six nearest neighbors) Initial structural information...
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Báo cáo y học: "ADAMTS proteinases: a multi-domain, multi-functional family with roles in extracellular matrix turnover and arthritis" pdf
... level of both expression and translation and that their structures and activities are regulated through mRNA splicing and post-translationally through protein processing and endogenous inhibitors ... W: Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc-binding environments (HEXXHXXGXXH and Met-turn) and topologies and should be grouped into a common family, ... [37] and arthritis [38], and specific ADAMTS proteins may have a role in its regulation ADAMTS-1 and -8 possess a potent angioinhibitory activity, suppressing both fibroblast growth factor-2 and...
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Báo cáo y học: "Regulatory polymorphisms in extracellular matrix protease genes and susceptibility to rheumatoid arthritis: a case-control study" ppsx
... the two SNPs in MMP7 (-181 A/G and -153 C/T), between the MMP1 -1607 1G/2G and MMP3 -1171 6A/5A SNPs, and between MMP13 -77 A/G and two other SNPs (MMP3 -1171 6A/5A and MMP7 -153 C/T) Comparison ... plasminogen activator, tissue type (PLAT) [17] and plasminogen activator inhibitor-1 (PAI-1) [18] They were selected as appropriate candidates to participate in RA susceptibility and studied in a large ... polymorphisms in matrix metalloproteinase-1 and monocyte Page of (page number not for citation purposes) 16 17 18 19 20 21 22 23 24 chemoattractant protein-1 and rheumatoid arthritis Scand J Rheumatol...
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Báo cáo y học: "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling" pps
... overexpression on mRNA levels of extracellular matrix molecules and proteoglycan (PG) synthesis overexpression on mRNA levels of extracellular matrix molecules and proteoglycan (PG) synthesis ... http://arthritis-research.com/content/9/5/R102 level and to stimulate cartilage extracellular matrix proteoglycan production [24,25] In this study, BMP-2 induced an increase in mRNA levels of collagen type II and aggrecan and stimulated ... levels of extracellular matrix molecules Cartilage of mice injected with either Ad-BMP-2 or Ad-luc was isolated after 3, 7, and 21 days Cartilage was pooled per group per time point, and RNA was...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... contributions HEG and ENH conceived the study and participated in its design and co-ordination HT and HEG wrote the manuscript HT, MK and RD performed all experiments and assays HT and RD retrieved tissues ... markers CD45 and CD34 The readily available antihuman markers CD90 and CD44 did not cross-react with sand rat tissue; thus they were not tested in the present study Since anti-sand rat antibodies ... cells and chondrogenesis Cytotherapy 2007, 9:712-6 Mwale F, Roughley P, Antoniou J: Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: a requisite for tissue...
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Báo cáo y học: "Human rheumatoid arthritis tissue production of IL-17A drives matrix and cartilage degradation: synergy with tumour necrosis factor-α, Oncostatin M and response to biologic therapies" ppt
... inflamed joints and to elucidate the mechanism of IL-17A, alone and in combination with TNF-α and oncostatin M (OSM) in matrix turnover and cartilage degradation using whole RA synovial tissue explants, ... (RASFC) and normal human cartilage cultures Furthermore, we examined the relation of IL-17A to matrix turnover in patients following anti-TNF-α therapy Materials and methods Synovial tissue, serum and ... evaluated before and at 1, and 12 months after initiation of biologic therapy Blood samples were obtained and sera was separated and stored at -80°C until used for biomarker analysis, and samples...
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báo cáo khoa học: " Induction of stromule formation by extracellular sucrose and glucose in epidermal leaf tissue of Arabidopsis thaliana" pot
... intracellular and extracellular sensing mechanisms operating in plants [15,27] Since sucrose is efficiently cleaved into fructose and glucose, both in the apoplast and in the cytosol by invertases and ... MHS designed and carried out all the experiments and wrote the manuscript RBK participated in the experimental design and helped to draft and write the manuscript Both authors read and approved ... Arabidopsis thaliana tissue culture medium We used vacuum infiltration of the leaf tissue to ensure fast and uniform exposure of cells to the solution At distinct time points (0, 60, 120, and 180 min),...
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báo cáo khoa học: " In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration" pptx
... optimal seeding density and culture period HGF, PDLF and HOB cells were trypsinized after serum starvation and suspended into standard culture medium with 10% FBS HGF and PDLF were seeded into ... and HOB cells on various GTR membranes [20-22] Locci et al [20] demonstrated that matrix membranes composed of collagen and chondroitin glycosaminoglycan enhanced cellular proliferation and extracellular ... gingival fibroblast DNA synthesis and caused a marked decrease in synthesis of extracellular collagen and glycosaminoglycan, the major components of extracellular matrix The authors proposed that...
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báo cáo khoa học:" Gene-enhanced tissue engineering for dental hard tissue regeneration: (2) dentin-pulp and periodontal regeneration" pptx
... techniques such as scaling and root planing and surgical procedures such as open flap debridement for access and resective techniques, are designed to remove diseased tissue and promote an ideal environment ... concentration of bone and periodontal-inductive agents (and hence biological activity) between different preparations b Guided tissue regeneration Guided tissue regeneration (GTR) is an approach to regaining ... Mol Cells 2005, 19:402-407 Goldberg M, Smith AJ: Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering Crit Rev Oral Biol Med 2004, 15:13-27...
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Báo cáo y học: " Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in threedimensional collagen gels" pps
... medium Matrix metalloproteases and (MMP-2 and MMP-9) Culture media were harvested after 5, 10, 15 and 21 days under control conditions and, after days, in the presence of neutrophil elastase (NE) and ... cultured alone released no gelatinolytic activity (data not shown) Prostaglandin E2 (PGE2) and matrix metalloproteases (MMPs) and Culture media were harvested after four days in the presence of neutrophil ... Immunoblot for matrix metalloprotease (MMP)-1 Media were harvested after 5, 10, 15 and 21 days under control conditions and, after five days, in the presence of neutrophil elastase (NE) and were subjected...
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Báo cáo y học: "Matrix metalloproteinase-9, -10, and tissue inhibitor of matrix metalloproteinases-1 blood levels as biomarkers of severity and mortality in sepsis." pptx
... JO and JAR carried out the determination of MPM-9 and TIMP-1 and made substantial contributions to the analysis and interpretation of data JMBL and YB carried out the determination of TNF-α and ... sepsis patients and 50 controls in terms of age and sex Higher serum levels of MMP10 (P < 0.001) and TIMP-1 (P < 0.001), and nonsignificantly Table Comparison of MMP-9, MMP-10, and TIMP-1 serum ... APACHE-II scores were 19 and 24 in surviving and nonsurviving patients, respectively; and in the study by Hoffmann et al [11], the mean APACHE-II scores were 14 and 23 in surviving and nonsurviving patients,...
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Báo cáo y học: "The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands. They are transmembrane α" ppsx
... transmembrane links between extracellular contacts (other cells or the extracellular matrix) and the actin microfilaments of the cytoskeleton, whose behavior integrins also regulate and modulate Many different ... in the ligand-binding pocket of the headpiece that increase its affinity for ligand The affinity directly regulates the nature of the ligand binding and appears to tune the degree and kinetics ... on binding extracellular ligands, mammalian integrins cluster in the membrane and transduce signals to the interior of the cell (outside-in signaling; Figure 4b) Extracellular ligand binding...
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