expressions white space semicolons and mini fi cation
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
Ngày tải lên :
18/02/2014, 13:20
... (Fig 2E) Puri cation of Ab(M1–40) and Ab(M1–42) The present work describes a rapid and inexpensive puri cation scheme to produce high-purity Ab(M1–40) and Ab(M1–42) in 24 h The puri cation scheme, ... (2% gel), and the cleaved product was purified using the GFX PCR and gel band puri cation kit The purified cut gene was ligated into PetSac vector (a modified from of Pet3a with NdeI and SacI cloning ... and simple expression and puri cation protocol will facilitate large-scale investigations of the molecular determinants of aggregation and fibrillation Given the intense interest in Ab, significant...
- 16
- 691
- 0
Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... activity, to provide new understanding concerning its bifunctionality and to establish links between this GH-57 amylopullulanase and other non pullulandegrading GH-57 and GH-13 amylolytic enzymes ... are shown and the mutated codon is underlined): Glu291Ala (5¢-CGG ATGGGCGGCTGCGAGCGCCCTCAAGAC-3¢) and Asp394Ala (5¢-GTGGTCACGCTCGCCGGCGAGAAC CCGTGGGAG-3¢) After mutagenesis and veri cation by ... members of GH-57 and exhibits an unusual specificity towards galactosidic bonds The alignment and mutagenesis strategy employed by van Lieshout et al [36] allowed the identi cation of Glu117 as...
- 10
- 577
- 0
Tài liệu Báo cáo Y học: Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3 potx
Ngày tải lên :
21/02/2014, 15:20
... and 2.05; dC 23.6 (2 Me), 175.9 and 176.2 (both CO) Sugar or amino-acid residue H1 H2 H3a, 3b H4 H5 H6a, 6b C1 C2 C3 C4 C5 fi 3)-b-D-GalpNAcI-(1 fi fi 6)-b-D-GalpNAcII-(1 fi › fi 4)-b-D-GlcpA-(1 fi ... 1869–1875 O’Hara, C.M., Brenner, F.W & Miller, J.M (2000) Classi cation, identi cation, and clinical significance of Proteus, Providencia, and Morganella Clin Microbiol Rev 13, 534–546 Stamm, W.E (1999) ... serum recognized slow migrating bands of three LPS (without R14) and fast migrating bands of P mirabilis G1, O28, and R14 LPS (Fig 5A) These bands correspond to high- and low-molecular-mass LPS species...
- 7
- 465
- 0
Project Gutenberg’s Space, Time and Gravitation pot
Ngày tải lên :
06/03/2014, 13:20
... space. — “For is and is-not though with Rule and Line And up -and- down without, I could define.” Alas! It is not so simple We abstract from the phenomena that which is peculiar to the position and ... default And after all, when you claim to determine lengths to eight significant figures, you must have a pretty definite standard of right and wrong measurements Phys It is difficult to define what ... whether the first one changes length; and a third to test the second; and so ad infinitum You remind me of the incident of the clock and time-gun in Egypt The man in charge of the time-gun fired it...
- 219
- 358
- 0
Báo cáo khoa học: Space, time and nitric oxide – neuronal nitric oxide synthase generates signal pulses pptx
Ngày tải lên :
07/03/2014, 00:20
... constants k1, k4 and k8 correspond to heme reduction; k2 and k5 correspond to oxygen binding; and k3 and k6 correspond to catalytic steps k7 and k9 correspond to the release of NO from ferric and ferrous ... k1, k4, k¢4 and k8 correspond to heme reduction; k2 and k5 correspond to oxygen binding; and k3 and k6 correspond to catalytic steps k7 and k9 correspond to release of NO from ferric and ferrous ... strain BL21DE, and purified using ammonium sulfate precipitation and gel filtration and 2¢,5¢-ADP Sepharose chromatography [43,44] Activity was measured by oxyhemoglobin assay, and was 500–700...
- 12
- 402
- 0
Hydrogen and Syngas Production and Purifi cation Technologies pdf
Ngày tải lên :
07/03/2014, 17:20
... catalytic partial oxidation of gaseous and liquid fuels, and gasi cation of solid fuels for efficient production of hydrogen and syngas and their separation and puri cation methods, including water-gas-shift, ... 4.6 4.7 Classi cation of Different Gasifiers 176 GE (Texaco) Gasi cation Technology with CWS Feeding 4.7.1 4.7.2 4.7.3 4.7.4 4.9 Introduction to GE Gasi cation Technology 178 GE Gasi cation Process ... Gasifier 184 Summary of GE Slurry Feeding Gasi cation Technology Shell Gasi cation Technology with Dry Feeding 187 4.8.1 Introduction to Dry-Feeding Coal Gasi cation 187 4.8.2 Shell Gasification...
- 564
- 4.3K
- 0
Báo cáo khoa học: "Contradictions and Justifications: Extensions to the Textual Entailment Task" ppt
Ngày tải lên :
08/03/2014, 01:20
... justifications For a given justi cation, a judge first assigned an integer score between 1–5 on how un1 Using the mean treats a score as an interval variable rather derstandable the justi cation ... from each justi cation run Brackets contain the pair of scores assigned to the justi cation by one of the six human judges; the first number in the pair is the understandability score and the second ... prefer different justifications After rating the 100 justifications, judges were asked to write a short summary of their impression of the task and what they looked for in a justi cation These summaries...
- 9
- 376
- 0
Báo cáo Y học: Novel fish hypothalamic neuropeptide Cloning of a cDNA encoding the precursor polypeptide and identification and localization of the mature peptide pptx
Ngày tải lên :
08/03/2014, 16:20
... markers (Roche Diagnostics) Immunoaffinity puri cation and mass spectrometry (MS) To identify endogenous mature peptides in the brain, we carried out affinity puri cation and immunoassay with the antiserum ... further ampli cation of the first-round PCR products with the anchor primer and gene-specific primer (5¢-AAA CCTTTGCGGTAGGGTGG-3¢, complementary to nucleotides 416–435) Both first-round and second-round ... characterized and subsequently a mature endogenous peptide was identified in the goldfish brain The localization of its transcript in the goldfish brain was further investigated 45 °C and 2.5 at 72 °C, and...
- 9
- 382
- 0
Solar and Space Physics and Its Role in Space Exploration doc
Ngày tải lên :
14/03/2014, 10:20
... effects of space climate and weather on humans, instrumentation, and communications and spacecraft systems To that end, input from space physics observations and analysis will help in modeling and developing ... National Aeronautics and Space Administration, The Vision for Space Exploration, NP-2004-01-334-HQ, NASA, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION FIGURE ... Solar and Space Physics and Its Role in Space Exploration Committee on the Assessment of the Role of Solar and Space Physics in NASA’s Space Exploration Initiative Space Studies Board...
- 74
- 369
- 0
Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism doc
Ngày tải lên :
15/03/2014, 10:20
... nontemplate strand involving two DM-azos was first irradiated with UV light in the single-stranded state to facilitate trans fi cis isomerization, and then annealed with the template strand to form ... Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No 21241031), by Core Research for Evolution Science and Technology (CREST), Japan Science and ... Technology Agency, Japan, and by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Nos 20750132 and 18001001) References Zhang...
- 11
- 436
- 0
Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
Ngày tải lên :
16/03/2014, 18:20
... was collected 48 h after the induction, centrifuged and culture supernatants were quickly frozen and stored at )20 °C prior to puri cation Puri cation of His-tagged Gas1 proteins A 10–20 mL sample ... CGCCGGTAATCAAGTTACTAACA-3¢) and reverse primer His-XHdown were used Similarly, for sGas1E262Q-H, the ampli cation was carried out using a pairing of the forward primer XHup and reverse primer Ó FEBS 2004 Production and ... is shown in italics) Plasmid DNA was purified using plasmid puri cation kits (Qiagen) DNA sequencing was routinely used to confirm the correct fusions and the absence of undesired mutations throughout...
- 11
- 435
- 0
Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
Ngày tải lên :
16/03/2014, 18:20
... (lanes and 6) and membrane fraction (3 and 7) of S pneumoniae strains Purified recombinant StkP was used as a control (lane 4) Arrows indicate bands corresponding to StkP Molecular mass standards ... separated and in the quantities sufficient for MALDI-TOF-MS identi cation Four phosphorylated proteins were identified being present in the wild-type as well as mutant strains (Fig 4, spots P3-6, and ... coli [22] and C glutamicum [15] The significance of its modi cation and nature of modifying enzyme remains unclear One of the phosphoproteins which is absent in mutant strain was identified as a-subunit...
- 12
- 466
- 0
Báo cáo khoa học: Structures of the O-polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups ppt
Ngày tải lên :
16/03/2014, 23:20
... 13 C H 13 C H 13 C 13 fi6 )-b-D-GlcpNAc-( 1fi fi2 ,4)-b-D-GalpA-( 1fi fi3 )-a-D-GalpNAc-( 1fi a-D-Glcp-( 1fi (6a,6b) 1.36 19.8 3.90, 4.17 69.9 174.3 3.73 62.3 3.66, 3.87 61.7 The 1H- and 13C-NMR spectra of ... RFMK-226.2003.03 and RF State Contract RI–112 ⁄ 001 ⁄ 272 References O’Hara CM, Brenner FW & Miller JM (2000) Classi cation, identi cation, and clinical significance of Proteus, Providencia, and Morganella ... 102.9 and 104.1 (d 101.9, 102.0, 102.7 and 104.0 in P penneri 25), two OCH2-C groups at d 61.8 and 68.7 (d 61.9 and 68.8 in P penneri 25), two nitrogenbearing carbons at d 55.4 and 56.1 (d 55.3 and...
- 8
- 349
- 0
Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
Ngày tải lên :
17/03/2014, 10:20
... in vitro, and purified To our knowledge, this is the first example of a flavodoxin refolded from inclusion bodies MATERIALS AND METHODS Materials Bacterial cultures, plasmid purifications and transformations ... and classi cation of hyperfine-shifted 1H, 2H, and 15H resonances of the Rieske ferredoxin component of toluene 4-monooxygenase Biochemistry 38, 727–739 27 Shaw, J.P & Harayama, S (1992) Puri cation ... Sigma All other chemicals were from Sigma Tomo D subunit was expressed and purified as described [13] The expression and puri cation of catechol 2,3-dioxygenase from P stutzeri OX1 will be described...
- 11
- 478
- 0
Relativity, space time and cosmology wudka
Ngày tải lên :
17/03/2014, 13:35
... universe through atoms moving randomly and colliding to form larger bodies and worlds He also believed that space is infinite and eternal, and that the number of atoms are infinite Democritus’s philosophy ... observations and experiments, and is the subject of the next section 1.2 The scientific method Science is best defined as a careful, disciplined, logical search for knowledge about any and all aspects ... taken seriously without independent veri cation Also, most scientists are idealists They perceive beauty in scientific truth and see its discovery as their vocation Without this most would have gone...
- 219
- 403
- 0
Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
Ngày tải lên :
17/03/2014, 17:20
... membrane with Coomassie blue R450, the protein band was cut out and subjected to N-terminal sequencing by gas-phase Edman degradation Generation and puri cation of internal peptides The puri®ed enzyme ... puri®ed and initially characterized previously [6] In this communication we report an improved puri cation protocol for the enzyme The gene encoding the protein was cloned, sequenced and glutamate ... is found in both subunits and differs considerably from other a helix/b sheet proteins including nucleotide-binding domains In this communication, we report the cloning and sequencing of the gene...
- 9
- 498
- 0
Báo cáo khoa học: Phylogenetic comparison and classification of laccase and related multicopper oxidase protein sequences pot
Ngày tải lên :
23/03/2014, 11:20
... and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability Biochim Biophys Acta 1292, 303–311 68 Tanaka N & Murao S (1982) Puri cation and ... in bacteria and their classi cation and function are still controversial The first report of a bacterial laccase was from the Gram-negative soil bacterium Azospirillum lipoferum [33] and the enzyme ... plant LMCOs on the basis of sequence similarity and phylogenetic analysis until specific physiological functions are defined They presented a classi cation of plant 2310 bacterial sequences with...
- 19
- 377
- 0
Báo cáo khoa học: Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution doc
Ngày tải lên :
23/03/2014, 11:20
... equations, resulting in a high affinity and low Vmax at low sucrose concentrations (Vmax1 and Km1) and low affinity and high(er) Vmax at high sucrose concentrations (Vmax2 and Km2) [13] In Table the ... and the proteins purified to homogeneity as described, and verified by electrophoresis followed by silver staining (results not shown) Kinetic analysis of the improved variants The kinetic profile ... (Asp286, Gln328, and Asp393), and the residues forming the salt bridge that closes the active site (Asp144 and Arg509) The central b-barrel is shown as solid strands depicted in yellow The figure was...
- 9
- 344
- 0