expression isolation and purification
Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents systems, the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ... (2H, m, Hb-2 and Hb-6 ) and 2.00 (2H, m, Hb-3 and Hb-5 ) The 13C NMR spectrum of compound displayed signals at d: 22.8 (C-3 and C-5), 27.5 (C-7), 28.8 (C-9 and C-10), 31.5 (C-2 and C-6), 32.9 ... not be separated from each other by CCC Since K2 and K4 were close to K3 and K5, respectively, curcumol (2) and germacrone (3), and curzerene (4) and 1,8-cineole (5) were hardly separated from...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
... literature and in the manufacturer's manual for the pMAL expression system (pMAL Protein Fusion and Purification System Manual, New England BioLabs); and (2) translocation of LASV GP1 and NP to ... Figure 2(DE3) cells transformed with construct pMAL-c2x:NP Expression and purification of LASV NP from E coli Rosetta Expression and purification of LASV NP from E coli Rosetta 2(DE3) cells transformed ... B GP1-MBP MBP 38 28 17 GP1 14 Expression and purification of LASV GP1pMAL-c2x:GP1 Figure gami cells transformed with construct from E coli Rosetta Expression and purification of LASV GP1 from...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... simplified purification via affinity chromatography and characterization of the enzyme Material and methods Enzymes, chemicals and media Pfu+-polymerase was obtained from Roboclon (Berlin, Germany) and ... P-450cam gene and its expression in Escherichia coli J Biol Chem 261:1158–1163 Völker A, Kirschner A, Bornscheuer UT, Altenbuchner J (2008) Functional expression, purification, and characterization ... shifting the culture to 20°C and 200 rpm for another 16 h of cultivation Gene expression analysis Gene expression analysis was performed with crude cell extract Samples standardized to cell amount...
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Isolation and expression analyses of dazl, vasa and sox3 genes in the seabass (lates calcarifer)
... Objectives Isolation and expression analysis of three genes vasa, dazl and sox3 in the seabass were the major objectives of this study In addition, seabass testicular culture was attempted and primarily ... B BA Fig.2 Vasa expression in the seabass by RT-PCR Expression of lcvasa is specific in the gonads of adult seabass (A) and no expression in other somatic organs Differential expression is observed ... target mRNAs such as oskar and nanos, and control their translation (Hay et al., 1990; Lasko and Ashburner, 1990) The Vasa homologs of Canenorhabditis elegans, glh-1, glh-2 and glh-4, have been shown...
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Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. Coli
... cultures were induced with varying concentrations of IPTG (0, 10, 25, 50, 100, 250, 500 and 1000 μM) and further expressions were made for h at 37 °C An equal amount of soluble protein extract was ... reached basal level and the wash was collected To elute bound protein, gradient was set to 50%B (50 mM Tris, 500 mM imidazole and 500 mM sodium chloride, pH 7.5) at 0.5 ml/min and the protein was ... the procedure of Tunon and Johansson, 1984 This protocol allows very sensitive detection (1–10 ng of protein per band) with negligible background staining 2.1 Results Expression of recombinant...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... purified proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared ... Phe150 and Tyr153, were revealed to be related to substrate binding and recognition of d-p-HPH and l-p-HPH by HYDJs, preferring d-p-HPH as substrate The bulky side chains of Phe63, Leu92, Phe150 and...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS Half maximal inhibitory concentration (IC50) and median lethal dose (LD50) values from HeLa cells and mice divided ... numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... carrying a GFP expression cassette could be introduced by lipofection and maintained for at least days after transfection as assessed by GFP expression (Fig 4E) These Fig Isolation and characterization ... induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolation and characterization of BECs and LECs We next ... these cells Mesenteric BECs and LECs (Fig 5A,B) were also obtained by the same strategy as illustrated in Figs and 4, and were treated with VEGF-A or VEGF-C (Fig 5C) MAPK and Akt phosphorylation...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and averaged ... 12768–12785 Pulver, S.C., Froland, W.A., Lipscomb, J.D & Solomon, E.I (1997) Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... column, equilibrated and eluted in 0.1 M Tris/HCl, pH 7.6 and several common protein–protein dissociation salts (1 M sodium chloride, M urea and M guanidinium chloride) and ionic detergents (CHAPS, ... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for...
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Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... structures of EcR and USP from L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ... – 80 68 34 51 48 53 A Fig mRNA expression profiles of LdEcR and LdUSP (A) LdEcR mRNA and LdUSP mRNA expression in the fat body (FB), gut (GUT), integument (INT) and whole body (WB) at day in the ... the whole bodies and tissues of last instar (4th) larvae and adults using TRIzolÒ (Gibco BRL, Grand Island, NY, USA) as described previously [13] BCIRL-Lepd-SL1 was also used for isolation of total...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... described in the Materials and methods phenolic compounds and alginates, etc and hence preserved the enzymatic activity of lamjapin But the efficiency of extraction was still low and the proteins could ... Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate J Biochem Biophys Methods 11, 13–20 Neuhoff, V., Stamm R & Eibl, H (1985) Clear background and highly ... proteins and also to the low efficiency of extraction Physical properties of lamjapin Lamjapin obtained from the Mono Q column migrated as a single band characterized by both 12% SDS/PAGE and isoelectric...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... Baier, M & Dietz, K.-J (1996) Primary structure and expression of plant homologues of animal and fungal thioredoxin-dependent peroxide reductases and bacterial alkyl hydroperoxide reductases Plant ... D600 0.5, and recombinant protein expression was induced by the addition of 100 lM isopropyl thio-b-D-galactoside After induction, cells were grown for 18 h at 37 °C, pelleted, and resuspended ... NcoI and BamHI, and cloned into the pET-8c vector Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas was a gift from  Á M Goldschmidt-Clermont (Universite de Geneve, Switzerland)...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
... c-terpinene and is therefore designated ClcTS (Fig 3B), C62 and M34 both produced limonene and are designated Cl(+)LIMS1 and Cl(+)LIMS2, respectively (Fig 3C,E) and D85 produced b-pinene and is designated ... Monoterpene synthases from grand fir (Abies grandis) cDNA isolation, characterization, and functional expression of myrcene synthase, (–)-(4S)-limonene synthase, and (–)-(1S,5S)-pinene synthase ... rich in serines and threonines and low in acidic and basic amino acids and about 45–70 amino acids long Usually they show only little homology The predictions using PREDOTAR and TARGETP indicate...
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Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
... 2-oxoacids and 2-Oxoacid dehydrogenase complex from the Archaea pyruvate [14] In the current paper, we report the cloning and expression of the E2 and E3 genes of the same operon from T acidophilum, and ... pET19b-E1a and pET28a-E1b, which, respectively, coexpress the a and b subunits of the T acidophilum E1, have been described previously [14] Construction of expression vectors pET28a-E2 and pET28a-E3 Expression ... genomic DNA and cloned into the pET28a expression vector, as described in Experimental procedures Expression of the E3 gene was carried out in E coli BL21(DE3) cells Small-scale expression trials...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... bands at about 42, 43 and 44 kDa were detected, while no bands were visible in chloroplast extracts (Fig 3) One could assume that in mitochondria the unprocessed protein, the mature protein and ... remained in the cytoplasm (Fig 4C) Expression studies on the RNA and protein levels and enzyme activities Arabidopsis plants were grown in the greenhouse for 10–45 days and all plant tissue above ground ... and C; pGFP-C fusion) Bright field images of the same protoplasts were made to visualize the protoplast’s cell membrane and the chloroplasts (B and D) No significant differences in either the expression...
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Báo cáo khoa học: "Discriminative Strategies to Integrate Multiword Expression Recognition and Parsing" docx
... T Baldwin and K.S Nam 2010 Multiword Expressions Handbook of Natural Language Processing, Second Edition CRC Press, Taylor and Francis Group M -H Candito and B Crabb´ 2009 Improving gene erative ... The lexical resources might be useful to discover new expressions: usually, expressions that have standard syntax like nominal compounds and are difficult to predict from the endogenous features ... expressions and their identification in texts; section presents the two different strategies and their associated models; section describes the resources used for our experiments (the corpus and...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... Cys22 and Cys82 were modified by alkylation, or replaced with alanine and valine, respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and ... library was then designed with randomized CDR3 loops, and displayed successfully on the surface of fd bacteriophages and panned for specific binding molecules [10] Third, and most recently we isolated ... CDR1 and CDR3 loops (Type2 VNAR) [5,11] Camelid VHHs typically possess disulphide linkages either between the CDR1 and )3, or CDR2 and )3 loops [13,14] Despite these similarities, the camel and...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... re-testing and validating the effects on cell growth by transforming W303.1C and plating the cells at 23 °C, 30 °C and 37 °C A cold-sensitive growth phenotype (where toxin is active and interferes ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... single stranded DNA ⁄ PEG method as previously described [28] The expression of Kar2SP-RTA wild-type and mutant ORFs from the pRS316 vectors was under the control of the GAPDH promoter and the...
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