expression but octacalcium phosphate crystal induced inos mrna and no production were independent of il 1b
Báo cáo y học: "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity" doc
Ngày tải lên :
09/08/2014, 06:23
... also failed to detect any deposition of human antibody in kidneys from any of the mice There was evidence of non-SLE-related pathology, namely, neutrophil infiltration of kidney glomeruli and of ... a stable expression system for production of recombinant B3 and its variants in Chinese hamster ovary (CHO) cells Materials and methods Assembly of 'supervectors' for expression The 'expression ... Kettleborough and Dr T Jones at Aeres Biomedical, Mill Hill, London Recombinant expression vectors – pG1D1 containing human immunoglobulin B3VH cDNA, and pLN10 containing human immunoglobulin VL cDNA – were...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... [75Se]Der p and this band could only be detected in the lung Autoradiograms of separated liver and thoracic lymph node proteins revealed a radioactive band of 25 kDa in liver and bands of 30 and ... distribution of radioactivity in sensitized and nonsensitized mice Sensitized and nonsensitized mice received an i.t instillation of [75Se]Der p 2, instead of the last aerosol challenge at day 30 and ... fluid, of which 40–80% were eosinophils after receiving HDM aerosol (Fig 1B) The nontreated healthy animals showed only a small number of leukocytes in BAL fluid,
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... groups Aliquots of 100 lL of this reaction mixture were used for each time point For determination of the thiol content the 0.1 mL samples were diluted with 800 lL of NaCl/Pi buffer and then 100 ... activity of Erv1p and mutated enzymes Activities of the purified enzymes were measured with dithiothreitol as an artificial substrate (for details see Materials and methods) Turnover numbers were calculated ... C133S and C159S always contained a substantially lower amount of protein-bound FAD than all other mutant proteins The FAD content of the mutants C30S and C33S were similar to that of the wild-type...
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Báo cáo hóa học: "Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma" potx
Ngày tải lên :
18/06/2014, 16:20
... administration of matured dendritic cells compared to DTIC in patients with metastatic melanoma [11] In this work, DC generated in GM-CSF and IL- 4 were matured with TNF-a, IL- 1b, IL- 6 and prostaglandin ... regression of cutaneous lesions (concomitant changes in pigmentation and nodularity) with continued dosing Resection of two nodules at weeks 24 and 36 showed no evidence of viable melanoma; it ... expansion of activated T-cells were added to the wells on days 1, 6, and 10 or 11 of the 14 day in vitro sensitization culture period Interleukin (IL) -7 and IL- 15 were added at a final concentration of...
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Báo cáo hóa học: " Are quantum dots ready for in vivo imaging in human subjects?" docx
Ngày tải lên :
22/06/2014, 18:20
... encapsulation provides stability over a much broader pH range [35] The third method maintains the native ligands on the QDs and uses variants of amphiphilic diblock and triblock copolymers and phospholipids ... reductions in photobleaching and low non-specific adsorption After conjugation with DNA, QDs were directly injected into Xenopus embryos and QDs were found to be diffusely distributed throughout the cell ... cell carcinoma in mice No significant photobleaching or degradation of QDs was observed even after an hour of continuous excitation The stability of QDs combined with their time resolution of optical...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
Ngày tải lên :
11/08/2014, 00:22
... the nanoparticles and not by nonspecific absorption, the solution of NIRF-labeled Hou et al Journal of Nanobiotechnology 2010, 8:25 http://www.jnanobiotechnology.com/content/8/1/25 Page 11 of 14 ... different doses of the Hou et al Journal of Nanobiotechnology 2010, 8:25 http://www.jnanobiotechnology.com/content/8/1/25 Page of 14 Figure Measurement of cell viability (A) NIRF signals of cells treated ... et al Journal of Nanobiotechnology 2010, 8:25 http://www.jnanobiotechnology.com/content/8/1/25 Page of 14 Figure Measurement of the relative iron-loading of cells (A) NIRF signal of cells labeled...
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báo cáo khoa học: "BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer" ppsx
Ngày tải lên :
11/08/2014, 00:23
... National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao ... 1052nm04100 and No 072112006-6) Wang et al Journal of Nanobiotechnology 2011, 9:23 http://www.jnanobiotechnology.com/content/9/1/23 Author details Department of Bio-nano Science and Engineering, ... Journal of Nanobiotechnology 2011, 9:23 http://www.jnanobiotechnology.com/content/9/1/23 Page of 12 Figure In vitro fluorescence images of MGC 803 after treated with FMNPs and FMNPs-BRCAA1 nanoparticles...
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Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa
Ngày tải lên :
13/08/2014, 09:21
... lentiviruses since for HIV-2 and SIV, sequences upstream of the splice donor predominantly contribute to mRNA packaging [9,10] and in FIV regions in U5 and in The ability of one lentiviral Gag to ... transfer vectors and Gag-Pol expression constructs are outlined in Figures and The constructs based on HIV-1 and SIV have been previously described [4,32] and were kind gifts of D Trono and K Uberla ... packaging ability of the Gag-Pol components of HIV-1, HIV-2 and SIV and found a unique non-reciprocal packaging relationship between SIV Gagpol and vectors based on HIV-2 In this paper the tropism of...
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Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc
Ngày tải lên :
13/08/2014, 13:20
... in capillaries of small animals The aim of the present study was to investigate whether the utility of a new and simple remission spectroscopy system allows reliable in vivo detection of liver ... levels of ALT and AST No significant changes between 3.0 h I/R and 6.0 h I/R animals were detectable When compared with 3.0 h I/R, mice treated with 6.0 h I/R and a continuous infusion of ET-1 ... (11.1) g) were used for all experiments The experimental protocols were in compliance with the guidelines of the Committee on the Care and Use of Laboratory Animals of the Institute of Laboratory...
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Báo cáo y học: "Human T-cell leukemia virus type 2 post-transcriptional control protein p28 is required for viral infectivity and persistence in vivo" ppt
Ngày tải lên :
13/08/2014, 05:20
... 6.5E+3 2.1E+3 } Copies p28 mRNA Figure permanent of p19 Gag and Expression transfectants Tax protein and p28 mRNA in Expression of p19 Gag and Tax protein and p28 mRNA in permanent transfectants ... vector and 0.1 μg of LTR-2-Luc, and 10 ng of TKRenilla or μg HTLV-2Δp28 and 0.1 μg of LTR-2-Luc, and 10 ng of TK-Renilla Cell lysates were harvested at 48 h post transfection and dual luciferase ... amino acid of the p28 ORF and had no affect on the overlapping Tax and Rex amino acid sequence We initially determined whether knocking out p28 altered Tax and/ or Rex activities Cotransfection of...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
Ngày tải lên :
08/08/2014, 17:20
... the body of evidence builds, the importance of iodine on the maintenance of healthy breast tissue and its role in carcinogenesis becomes clearer Unveiling iodine’s mechanism of action is of crucial ... Methods and Materials for details) RNA was isolated and subjected to Microarray Analysis (see Methods and Materials for details) 29 genes were upregulated ≥ 2.0-fold (A) and 14 genes were down ... effects of iodine in breast cancer pathways will improve our understanding of breast cancer physiology and potentially lead researchers toward the development of novel treatments or enhancements of...
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Báo cáo y học: ""Shock and kill" effects of class I-selective histone deacetylase inhibitors in combination with the glutathione synthesis inhibitor buthionine sulfoximine in cell line models for HIV-1 quiescence" doc
Ngày tải lên :
12/08/2014, 23:21
... proportion of cells expressing GFP, and a correct estimate of the expression of this protein at subsequent time points was biased by the autofluorescence of dying cells The A1 clone, which does not ... replication and cell viability of class I-selective HDACIs, MS-275 and buthionine sulfoximine (BSO), alone or in combination Effects Effects on HIV-1 replication and cell viability of class I-selective ... expression of green Stimulation ofor in combination in a Jurkat cell clone (A1) fluorescent protein (GFP) by MS-275 and buthionine sulfoximine Stimulation of HIV-1 LTR-controlled expression of...
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INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
Ngày tải lên :
02/10/2015, 17:15
... Levels of major pro-nociceptive cytokines IL- 6, IL- 1β and TNF-α were shown to be elevated, while that of interleukin-10 (IL- 10), an anti-inflammatory cytokine, were reduced in the CSF of patients ... the nociceptive response of mice to phenylbenzoquinone (Mnich et al., 1995) The nociceptive response was associated with the production of prostaglandins and was blocked by inhibitors of prostanoid ... associated with the development of pain In response to peripheral nerve injury and spinal nerve injury, TNF, IL- 1 and/ or IL- 6 mRNA expression and protein levels were shown to be elevated in the...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
Ngày tải lên :
02/11/2012, 11:17
... stimulation of HBV viral proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation ... cells by Northern Blot (Figure 1B, lane 2), despite the cIAP1 expressing level was not stronger as cIAP2 The increase of the expression of cIAP2 and cIAP1 in the HepG2.215 cells were not due to ... However, the low expression of XIAP in the samples (negative intensity of the dot) probably lessens the significant of this difference We will discuss this in detail late The expression of two other...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II, CD1d, ICAM-1 and the novel CD8 ligand gp180 We ... panel) and nonbiotinylated (lower panel) HepG2 cells grown in media without (–) and with (+) iron were immunoprecipitated with Nor3.2 antibodies Biotinylated and nonbiotinylated samples were boiled ... cells processed for immunocytochemistry, immunoprecipitation and mRNA studies (A) Cytospins of HepG2 cells were incubated with Nor3.2 followed by rabbit anti-(mouse Igs) and the alkaline phosphatase-antialkaline...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... the selective identification of more basic isoforms of eEF1A in cancer cells, but not in normal lymphocytes Materials and methods Oligonucleotides Oligonucleotides were purchased from MWG Biotech ... analysis of other samples, confirming the reproducibility of data obtained Thirty micrograms of nuclear extracts from normal human lymphocytes were similarly analysed by bidimensional PAGE and compared ... electrophoresed at 10 V cm)1, at a temperature of °C In the supershift gel-mobility assay, samples of total nuclear extracts were diluted : (v/v) in water and 0.5 lg of protein was incubated for 2.5 h at...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
Ngày tải lên :
21/02/2014, 00:20
... used for quantitative measurement of the protein bands The relative expression of PKB was calculated by the intensity ratio of PKB band and b-actin band Assay of PKB activity PKB assay was performed ... mAbs of the adhesion to HUVECs of untreated control and insulin treated cells (B) Inhibition by monoclonal antibodies of the migration and invasion of insulin treated H7721 cells Non, Cells not ... level of FucT-VII mRNA expression in insulin treated cells was 188.6% of the untreated control level, while in LY294002 treated and AS-PKB transfected cells, it was only 43.8% of UnC and 25.5% of...
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Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology pdf
Ngày tải lên :
06/03/2014, 09:22
... that were tested in order to arrive at the final standard: (a) a glutamate concentration of 75 mm and compensation of the remainder with 163 mm phosphate; (b) a phosphate concentration of 50 mm and ... rate of 0.5 mLÆmin)1] The fluxes through the enzymes of the glycolytic and fermentative pathways were calculated from steady-state rates of glucose consumption, and ethanol and glycerol production ... However, addition of carbonate to the assay medium is not practical, because of its instability Amino acids and nucleic acids form substantial groups of anions in the cell We focused on amino acids to...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
Ngày tải lên :
07/03/2014, 00:20
... synthesis, were normalized against expression of a control gene, ILS1, and normalized expression levels were scaled to give a wildtype value of for all tested genes Error bars indicate the SD of mean ... levels were quantified by real-time PCR after cDNA synthesis and normalized against transcript levels of a control (ILS1) to obtain normalized GAL expression Normalized GAL expression levels were ... those genes that were not induced at this time-point We conclude that a subset of Gal4-inducible genes were induced under our conditions, and that a further subset of these genes were SWI ⁄ SNF...
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Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx
Ngày tải lên :
07/03/2014, 00:20
... Drosophila [21] No nonspecific defects were observed in gonads of oysters injected with oyvl-dsRNA, and no oyster mortality was recorded during RNAi experiments, indicating that dsRNAs were not toxic ... development Knockdown of Oyvlg mRNA and protein expression A 70% inhibition of mRNA level after dsRNA treatment was considered to be a threshold for effective RNAi [23] In our data, a ‡ 70% reduction of ... levels of og-TGFb mRNA, specifically expressed in auxiliary cells of the germ cells [27], were 0.54 ± 0.20 for controls, 0.69 ± 0.30 and 0.59 ± 0.17 for oysters injected with 20 and 100 lg of oyvl-dsRNA,...
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