... on speaking and its impacts on speaking ability of the non -major ability of the non -major students at the post-students at the post-elementary level in Military elementary level in Military ... the participants Leadership moves - One or two students seem to be leaders in every talk Individual Presentation FindingsIndividual Presentation Findings most of the participants in ... 2 No planning groups & 2 Pre-planning groups)Records: All the group discussions and the individual presentations from No planning group & Pre-planning group Design of the studyDesign...
... ND(–)IRES 239 49 ± 8 40 ± 2 37 6 ± 31 (–)IRES 219 19 ± 4 13 ± 3 290 ± 30 (–)IRES 104 24 ± 3 25 ± 2 33 0 ± 30 (–)IRES 2 0 ND No bindingBinding and replication of 3 -end of HCV minus RNA T. Astier-Gin et ... made on the basis of the structure of the 5ÂUTR of the plus-RNA, the structure of the3 -end of the minus-strand RNA being at that time unavailable. In the present study we investigated the involvementof ... identify the role of this stemloop in NS5B binding and RNA synthesis from the HCV minus-strand RNA.Altogether these results suggest that several domainsof the RNA fragment comprising the 34 1 nt...
... the amount of enzyme that unwinds 30 % of the DNA helicase substrate at 37 °Cin30min(1 %in one min). For examining the effect of DNA-interactingcompounds on DNA unwinding activity of PDH120, the compounds ... February 20 03, accepted 20 February 20 03) Eur. J. Biochem. 270, 1 735 –1745 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 035 32.x In order to understand the mechanism of DNAunwinding we tested the ... inthe3 5Â directionand the others inthe 5 3 direction with respect to the strand on which they initially bind. Most organisms encodemultiple DNA helicases because of their involvement in numerous...
... totalcrystallinity(± 3% )0 13. 9 87 13 93 7 424 20.6 69 31 67 33 34 14 22.9 59 41 67 33 34 24 22.5 55 45 67 33 32 aADP-glucose is known to be unstable at high pH inthe presence of MgCl2(reviewed in ... the latter certainly does not exceed the rate ofamylopectin synthesis. The increase in crystallinity wit-nessed during these first 12 h is in line with the high ratesobserved for amylopectin ... amylose%ofcrystallinity( 3% )Amylosesynthesisrate (lgặh)1)Amylopectinsynthesis rate(lgặh)1)0 2.1 5 63 < 2b25 NA12 13. 4 5 63 < 2b 35 NA 94248 15.8 572 3. 6 34 16 5158 23 574 10 .3 32 180...
... protein binding the MARCKS 3 -UTR in Swiss 3T3 cells. In contrast to the Hu antiserum, no interaction of the CstF64 control antibody with the MARCKS RNA-bindingproteins was observed revealing ... proteinsand the3 -UTR of the MARCKS mRNA. (A) The MARCKS 3 -UTR, the stop codon UAA of the coding sequence (CDS) and the poly(A)sequence are depicted. The box within the3 -UTR marked the iden-tified ... we investi-gated whether there is a difference in expression of theseproteins in neural PCC7-Mz1 cells and Swiss 3T3 fibro-blasts. Only the HuR protein of 36 kDa was detected in Swiss 3T3 extracts...
... contrast, partof the incorporated CFMYP is not processed toEGMYP, enabling it to retain zinc. Indeed, 33 % of the total zinc inthe ovary at stage 1 and 13% of the total zinc inthe testis at stage ... metallothioneins, zinc-binding proteins that function asreservoirs for zinc in various animal cells [34 ], includ-ing sea urchin eggs [33 ].We do not exclude the possibility that MYP is alsoinvolved in ... suggesting that MYP inthe ovary binds more zincthan that inthe testis. Inthe ovary at stage 3 (mid-gametogenesis), zinc eluted coincidentally withMYP, but the peak was smaller than that in the...
... 4000c4297c 63 [36 ] 63 E7Ado 2 03 ± 97 216 ± 61 2.8 [35 ] 2.8 (Ado)Adenosine kinase 220 (ATP) [35 ] 220 (ATP)200 (AMP) [35 ]1200 (ADP) [35 ]E8ATP 200c 33 33 X 200c100 100ADP 4000c 33 33 X-P ... on the rate of synthesis ofinosine. Application of thetheoretical modelwas performed with the MATHEMATICA -3. 0program, as described inthe text and in [3] .Simulation was made considering the ... and the increase in inosine, promoted by H2O2, cannot be explainedsolely by a change inthe level of the enzymes more directlyinvolved inthe nucleotide pathway from ATP to inosine(Fig. 3) ,...
... mRNA insta-bility and the involvement of the ARE in this process[10,11]. In addition, another destabilizing sequence wasidentified within the coding region of IFN-b mRNA [12, 13] .Whereas the independent ... cells [ 13] , further supports the involvement of this protein inthe deadenylation processof IFN-b mRNA. Interestingly, IFN-b ARE does notrecruit other ARE-binding factors (data not shown),thereby ... (Fig. 3) . The CRID was identifiedpreviously as an instability determinant that, in combina-tion with the3 UTR, mediates the rapid decay of humanIFN-b mRNA in NDV-infected NIH/3T3 cells [ 13] . Bothsequences...
... elegans germline transformation was per-formed by coinjecting the vector construct 3- ureidopropion-ase::GFP with the pRF4 plasmid encoding the dominantmarker gene rol-6 into the germline of young ... livertissue [10 ,33 ]. In C. elegans, the tissue that performs‘liver-like’ function is the intestine. Therefore, the absence of 3- ureidopropionase expression is unex-pected. Presuming that there really ... (data not shown). Wetherefore conclude that the recombinant protein showsa genuine 3- ureidopropinase activity, confirming that the gene product of F13H8.7 is the C. elegans 3- urei-dopropionase.T....
... CBP–C2(2 036 –2 231 ); CBP–C3(2 231 –2441); CBP–C(1892–2441); p300–N(1–596); p300–M(744–1571); p300–C1(1855–2010); p300–C2(2010–2210); p300–C3(2210–2414); andp300–C(1571– 237 0). The intensity of binding, ... y divid ing t he foldinduction obtained experimentally for a given combination of proteinsby the value obtained when the fold induction for each of the proteinspresent inthe comb ination ... plasmids,P31·2CAT and )110IFNbCAT. P31 is the binding site forIRFs inthe IFN- b gene promoter (Fig. 1A) and P31·2CATcontains two copies of P31 d riving the expression of the CAT reporter through the...
... binding totitin by improving the accessibility of the titin-bindingdomain. Interestingly, the importance of CARP inter-action for its function was recently demonstrated in aFig. 3. Calpain 3 ... resulting from the mating ofclones transformed with either calpain 3 or CARPgrow on Leu-Trp-His- selection medium, indicatingthat calpain 3 and CARP interact (Fig. 1B). The factthat calpain 3 interacts ... ankyrin repeats inthe inhibition ofDNA binding activity. Proc Natl Acad Sci USA 89, 433 3– 433 7.61 Perkins ND (2006) Post-translational modifications reg-ulating the activity and function of the...
... knowncomplexes, the lipidic chain stretches out of the bind-ing pocket with the polar head group protruding out,facing the solvent [45], with no interactions with the protein. This may explain why the ... methyl group binds, surrounded byhydrophobic side chains from the protein. Thus, the binding site results in a polarized cleft inthe interiorof a basic container with two main openings to bulksolvent, ... tyrosine residues inthe hydrophobic cavityof this protein. Alternatively, the binding of a sub-strate can be demonstrated by the stabilizationinduced on the protein. Inthe absence of reducingagents,...
... on the asymmetryof the active site that forces the binding of the sub-strate inthe monoanionic form [38 –41]. The decrease in Kmvalues of mutant T249G C2,3O on 3, 5-DMCand 3, 6-DMC could indicate ... 2 37 ± 3 63. 6 ± 5 3- MC 3. 8 ± 0.4 11.5 ± 1 14 .3 ± 1 26.6 ± 3 3,5-DMC 73. 8 ± 6 57.5 ± 4 38 .1 ± 3 23. 7 ± 2 3, 6-DMC 21.5 ± 2 9.7 ± 1 5.5 ± 0.6 7.4 ± 0.6Catechol 180 ± 11 170 ± 10 48 ± 3 47.7 ± 3 kcat(s)1) ... Appar-ently, the close contacts between these two residuesand the edge of the substrate ring could prevent bind-ing of 3, 6-DMC and 3, 5-DMC, as shown in Fig. 2-D,E,F. Thus, the binding of 3, 5-DMC and 3, 6-DMCto...