ex vivo measurements of mucus secretion by colon explants
báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... related expansion of CD4+/CD25+/ CD127-/FoxP3+ regulatory T cells IL-2 is critical for the ex- vivo expansion of suppressive regulatory cells [25] Using high doses of IL-2 in our NK cell expansion ... enhanced by the presence of 3a-G1 Conversely, the addition of 3a-G1 to cultures induces a massive and significant reduction of the expansion of CD4+ T cells At the donor level, a higher proportion of ... the proliferation and the expansion of T cells was maintained in the presence or absence of exogenous IL-2 (Fig 3b) Thus, monocytes are involved in the ex- vivo priming of autologous CD4+T cells...
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In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties pps
Ngày tải lên :
10/08/2014, 05:21
... Where: Tb: time of passage of the cell suspension through the filter; Ts: time of passage of an equal volume of PBS; Htc: hæmatocrit (10%) Page of The erythrocyte deformability measurements are ... interpretation of data of erythrocyte membrane fluidity estimated by electron paramagnetic resonance spectroscopy (EPR) MEG: acquisition, analysis and interpretation of data of erythrocyte aggregation of ... of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments RV and SP: protocol design and obtention of the consent...
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báo cáo khoa học: "Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells" doc
Ngày tải lên :
10/08/2014, 22:20
... Phenotype of day-14 ex vivo expansion cultures: Liquid cultures for 14 day ex vivo expansion were established with 100,000 CD34+ cells in 50 ng/ml of SCF, TPO and Flt-3 Ligand The results of six ... days of culture, cells were harvested to analyze CD34 and CD52 expression profiles A: Expression profiles of CD34 and CD52 at Day and Day 14 of secondary cultures B: Absolute expansion fold of ... numbers Immunohomeostasis in in vivo transplantation/engraftment are far more complicated that just an ex vivo study and the results of ex vivo expansion caused by addition of alemtuzumab this study...
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Báo cáo y học: "Ex vivo effects of flavonoids extracted from Artemisia herba alba on cytokines and nitric oxide production in Algerian patients with Adamantiades-Behcet''''s disease" pptx
Ngày tải lên :
11/08/2014, 03:20
... department of botany of the national institute of agronomy in Algeria Flavonoùds were extracted according to the extraction method described previously by Paris and Nothis [36] Briefly, 20g of the ... assessment of the biological activity of this extract in vivo and on the chemical identification of the active components responsible for the anti-inflammatory activity The knowledge of the role of flavonoùds ... determined by ELISA (Fig.4) Treatment of PBMC by different concentrations of flavonoùds inhibited IL-4 production (73.26 10 pg/mL , 30 àg/mL of flavonoùds) and (89.90 13.25 pg/mL, 50 àg/mL of flavonoùds)...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
Ngày tải lên :
09/10/2015, 11:24
... processing was completed using software supplied by the confocal microscope’s manufacturer 2.6 2.6.1 In vivo experiments Culture of H1 cells in Matrigel For in vivo experiments H1 cells cultured ... before implantation in vivo 2.6.4 In vivo grouping A total of SCID mice were used for in vivo experiments, with each mouse carrying - samples of scaffold-cell constructs Three of the mice carried ... induction of supportive factors secretion in MEF, up-regulation of major genes expressed in TGFβ/activin/nodal signaling and inhibition of BMP signaling (Gerber et al, 2007) The activation of the...
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Photoimmunotherapy of melanoma via combination of hypericin photodynamic therapy and in vivo stimulation of dendritic cells by PNGVL3 HFLEX plasmid DNA
Ngày tải lên :
28/11/2015, 13:45
... drawback of ex vivo DC-based vaccines is that the ex vivo production of individually tailored cellular therapies is laborious and costly Hence, the use of in situ approaches which take advantage of ... Levy, 2005) The in vivo expansion and generation of mature DCs offers many other potential theoretical advantages over the traditional in vitro culturing of DCs In vivo expansion of DCs has the potential ... modality of treatment is the main focus of the present study and will be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of...
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Báo cáo y học: " Restriction by APOBEC3 proteins of endogenous retroviruses with an extracellular life cycle: ex vivo effects and in vivo "traces" on the murine IAPE and human HERV-K elements" pot
Ngày tải lên :
13/08/2014, 05:21
... most of these elements are no longer active due to the accumulation of inactivating mutations, some of them are still functional and have been cloned, thus allowing direct ex vivo assay of the ... proteins in the ex vivo assay above, we asked whether APOBEC3 proteins might have actually impaired the in vivo amplification of these elements in the past, by searching for evidence of APOBEC3-editing ... been observed, both in ex vivo assays and by in silico analysis of the traces that APOBEC proteins have left through DNA edition in the course of reverse transcription of the retroelements [26]...
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Báo cáo y học: "Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination" ppsx
Ngày tải lên :
13/08/2014, 05:22
... either by one, or by a combination of both mechanisms In this context, we were able to produce 10.5% of recombinant gag sequences in a separate ex vivo experiment by in vitro transcribing different ... HML-2 variants exist in humans to this day [13,14] If it still existed, the exact nature of that variant, in terms of its actual sequence, remains unspecified We report here analysis of mysterious ... switch templates both in vivo and ex vivo [1620] While template switches of retroviral RT's are critical for retrovirus biology, RT template switches occuring ex vivo and thus producing sequence...
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Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc
Ngày tải lên :
19/02/2014, 08:20
... a similar extent Of particular interest from the point of view of amyloid disease is the characterization of the two new mutational variants associated with clinical disease Analysis of both the ... corresponds, within the bounds of experimental error, to the Tm determined by CD analysis at pH 5.0 (Table 1) Because of the low protein concentrations of F57I and W64R, measurement of the ANS fluorescence ... with Tm ANS values lower by 18 ± °C than that of the WT protein Moreover, examination of the location of the naturally occurring mutations in the native structure of lysozyme shows that the...
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Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc
Ngày tải lên :
07/03/2014, 16:20
... not shown) As expected, the amount of Gly-extended CCK, which requires the action of Kex2p and Kex1p in the Golgi apparatus is unchanged in all four expression constructs as a result of the limited ... cleavage of synthetic human CCK-33 to CCK-22 was analysed by inclusion of a number of inhibitors with the extract from BJ2168 Only the addition of a metal chelating agent inhibited proteolysis of CCK-33 ... numbering of the amino acid residues refers to proCCK, of which the release of Gly-extended and C-terminal extended CCK-22 requires cleavage at the C-terminus of Lys61 (equals Lys81 of preproCCK)...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
Ngày tải lên :
08/03/2014, 22:20
... In vivo inactivation of the yeast plasma membrane ATPase in the absence of exogenous catabolism Biochim Biophys Acta 1151, 83–88 ´ Rosa, M & Sa-Correia, I (1991) In vivo activation by ethanol of ... vortexed Miscellaneous Released phosphate was determined by the method of Onishi [26] Protein concentration was determined by the method of Bradford [27] using thyroglobulin as the standard Except ... unsaturation of complex lipids Ó FEBS 2002 ´ 1008 A Hernandez et al (Eur J Biochem 269) Fig H+-ATPase activation by the 18 : 2/18 : ratio is nonessential À1 À1 Plots of KmV max and V max vs the ratio of...
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Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc
Ngày tải lên :
16/03/2014, 06:20
... Phosphorylation of Ser47 in the propeptide of PCSK9 is decreased by the naturally occurring R46L and A53V PCSK9 variants We next examined the effect of several hPCSK9-V5 variations on the phosphorylation of ... analysis of the consensus site of PCSK9-propeptide phosphorylation from the media of transfected Huh7 cells overexpressing V5-tagged PCSK9 variants (A–H) TOF-MS analyses of the propeptide of V5-tagged ... analysis of the PCSK9-propeptide molecular mass heterogeneity (A–D) TOF-MS analyses of the molecular forms of endogenously expressed PCSK9-propeptide immunoprecipitated from the media of HepG2...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
Ngày tải lên :
17/03/2014, 17:20
... NMR signals of glycogen and glucose in vivo were quantified by comparison with the measurements of phantoms containing solutions of % 400 mM natural abundance oyster glycogen and 0.9 mM of 99% enriched ... were assessed in vivo and in phantom experiments Assessment of the overexpression of the double mutant bifunctional enzyme The adenovirus infusion resulted in overexpression of the bifunctional ... Previously, we reported that the levels of Fru-2,6-P2 were Ó FEBS 2002 significantly increased by adenovirus-mediated overexpression of a mutant form of 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... (+HSD+C.I.1) of 25 lM C.I.1, were submitted to 8% SDS-PAGE followed by immunoblotting Desmin and talin were detected with specific mAbs involved in such complexes by submitting samples of crude extracts of ... complexes of NOS with HSP90 are crucial in determining the in vivo resistance of NOS and HSP90 to calpain degradation Results Levels of HSP90 and NOS isozymes in rat brain and aorta The level of ... and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, together with traces of eNOS (Fig...
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Báo cáo hóa học: "Effect of montelukast on platelet activating factor- and tachykinin induced mucus secretion in the rat" doc
Ngày tải lên :
20/06/2014, 00:20
... tracheal explants Exp Lung Res 1991, 17:1079-94 Adler KB, Schwarz JE, Anderson WH, Welton AF: Platelet activating factor stimulates secretion of mucin by explants of rodent airways in organ culture Exp ... increase of mucus secretion up to 205 ± 49% of baseline levels The addition of montelukast (10 µM) to the culture medium had no significant effect on the secretion levels (95 ± 6%) Combination of PAF ... pathway by administration of dexamethasone or inhibitors of the lipoxygenase or cyclooxygenase pathway completely blocked the secretagogue properties of PAF [7,13,21] Furthermore, the experimentally...
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Báo cáo hóa học: " Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae" pptx
Ngày tải lên :
20/06/2014, 01:20
... activity of these deletion constructs was measured by firefly luciferase expression and normalized by the Renilla luciferase expression Both pGL3-2.6k and pGL3-2.2k constructs had luciferase expression ... [23,24] These in vivo findings and our current ex vivo characterization of the hsc70B regulatory region unequivocally indicate that the induction of HSC70B may be a mosquito Page of (page number ... ing of a block of three repeats of a 5-bp sequence, 5'nGAAn-3' Although the number of HSE blocks can vary among different HSPs, the 5-bp HSE repeat is highly conserved in the regulatory region of...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
Ngày tải lên :
09/08/2014, 03:24
... ‘professional’, contactdependent, regulatory T cells The existence of thymus-derived suppressor cells was suggested by studies in mice where neonatal thymectomy on day led to the development of ... abolished by anti-TGF-β, but for one exception [19] Nakamura et al reported that immunosuppression by CD4+ CD25+ regulatory T cells is mediated by cell surface-bound TGF-β [19] Many of these differences ... amount of IL-2 is produced to overcome the inhibitory effects of TGF-β, the costimulatory effects of this cytokine induce the precursors of CD4+ CD25+ T cells to become contact-dependent ‘professional’...
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Báo cáo khoa học: "Biological in-vivo measurement of dose distribution in patients’ lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate gland" ppsx
Ngày tải lên :
09/08/2014, 09:20
... of 5% in 79% of lymphocytes (DLH), whereas only about 5% of the body volume receives the same dose (DVH) Doses of above 100% can be observed in the DLH, too This phenomenon can be explained by ... with the rest that hadn’t been exposed to radiation Non-exposed controls were also taken before radiation The protocol of staining gamma-H2AX by indirect immunofluorescence is published in many ... nucleus were counted by eye because of the possibility to focus manually through the whole nucleus by microscope to detect each focus in the 3D-room All experiments were counted by one and the same,...
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Báo cáo y học: " Antiretroviral activity of the aminothiol WR1065 against Human Immunodeficiency virus (HIV-1) in vitro and Simian Immunodeficiency virus (SIV) ex vivo" pptx
Ngày tải lên :
10/08/2014, 05:21
... effect of WR1065 on SIV replication ex vivo At the time of blood collection, the animals (612, 642 and 674), had plasma titers of 0.10, 0.03 and 6.40 × 106 copies of SIV RNA/ml, and CD4 counts of ... (non-apoptotic), with the majority of experiments showing 85-95% of the cells as Annexin-V negative (data not shown) Table also presents mean values for replicate experiments in which we exposed HIV-1-infected ... Biosciences) software Cell cycle analysis was performed using ModFit software (Venty Software, Topsham, ME) Percentages of cells in G0-G1, S and G2-M phases were calculated directly by the software...
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