... spectrum of the turnover form of the T2Cu center are slightly different from that of the as-puried form (cf Table 2) which suggests the existence of modications in the catalytic center of the enzyme ... recent model for the catalytic mechanism of Cu-Nir supposes that nitrite binds to the oxidized form of the T2Cu center, displacing a solvent molecule and forming a hydrogen bond between one of its ... allowed, for the rst time, the determination of the redox potentials of both copper centers Our studies suggest that the presence of substrate plays an important role in the modulation of the redox...
... moreover attach most often to the lowest, nearest N P is important for the semantic interpretation of sentences because of the combinatorial explosion of possible attachments of prepositional phrases ... user-adviser dialogues and Wizard .of- Oz condition to refer to the d a t a of adviser's our s t u d y Completeness and of Formality Users' Utterances As can be seen in Table I, for completeness (i.e., ... resemble more spoken informal discourse than written formal discourse The difference in number of occurrences per I000 words between the Wizard -of- OZ condition and the Informal Spoken condition...
... capable of stimulating an immune response in BD Most tissue studies of the pathophysiology of BD are conducted in skin One of the unique and diseasespecific features of BD is the occurrence of a ... immunopathologic characteristics of juvenile-onset spondylarthritis and other forms of juvenile idiopathic arthritis Arthritis Rheum 2006, 54:2594-2604 Page of (page number not for citation purposes) ... Authors’ information The authors of this editorial include a PhD resident (JK) and internist-immunologists (JVL, PVD, and PVH), representing the staff of the clinical immunological section of the...
... [23] were performed and a large set of treatments (e.g., Irwin et al Journal of Nanobiotechnology 2010, 8:34 http://www.jnanobiotechnology.com/content/8/1/34 Page of 12 11 levels of [Np]s {5, ... which was freshly made from the same lot of BHI powder Because of this we performed another set of experiments (Figure 2) to specifically clarify the effects of both media (2A: fresh BHI; 2B: stored ... both sides of this relationship are divided by n, Eq is produced Eq is just a rearrangement of Eq solving for CI and assuming T = hr For S aureus CF is typically ca 108 CFU mL-1 and for the Gram-negative...
... Polson, MT Placitas, NM Navajo, NM Navajo, NM Navajo, NM Fort Collins, CO Ault, CO Fort Lewis, CO Fort Lewis, CO Fort Lewis, CO Fort Lewis, CO Fort Lewis, CO Nathrop, CO Nathrop, CO Nathrop, CO Nathrop, ... Amplicons of a 751 nt region of the S segment and of a 142 nt region of the M segment were produced Nested PCR was then performed to produce samples used as sequencing templates Page 13 of 16 (page ... segments F test of neutrality (Fu and Li, 1993) for the M and S segments Only sequences from clade of the M tree (Figure 3) and clades – of the S tree (Figure 4) were analyzed Page 12 of 16 (page...
... Polson, MT Placitas, NM Navajo, NM Navajo, NM Navajo, NM Fort Collins, CO Ault, CO Fort Lewis, CO Fort Lewis, CO Fort Lewis, CO Fort Lewis, CO Fort Lewis, CO Nathrop, CO Nathrop, CO Nathrop, CO Nathrop, ... Amplicons of a 751 nt region of the S segment and of a 142 nt region of the M segment were produced Nested PCR was then performed to produce samples used as sequencing templates Page 13 of 16 (page ... segments F test of neutrality (Fu and Li, 1993) for the M and S segments Only sequences from clade of the M tree (Figure 3) and clades – of the S tree (Figure 4) were analyzed Page 12 of 16 (page...
... Page of (page number not for citation purposes) Retrovirology 2007, 4:11 http://www.retrovirology.com/content/4/1/11 lines frequently harbor more than one copy of the virus, although often some of ... the start of the pol ORF is underlined in the second region (site of ribosomal frameshift) lates A common feature of the Deltaretrovirus genus is the use of proline tRNA as a primer for the complementary ... Komischke and Rita Lippoldt for their skillful technical assistance They are indebted to Prof Harald Stein (Head of the Dept of Pathology, Charité Campus Benjamin Franklin) for giving them access...
... applied for the investigation of concrete structures This increase in the application of NDT methods is due to a number of factors: • technological improvements in hardware and software for data ... increased use of NDT methods is occurring despite the lack of testing standards for many of the methods The development of testing standards is critical for proper application and expanded use of NDT ... Ultrasonic-echo method—Some of the drawbacks of the through-transmission method are the need for access to both sides of the member and the lack of information on the location (depth) of a detected anomaly...
... binding for M2+ (M ¼ Zn, Cd) initially to the a domain, thereby leading to formation of the M4a cluster prior to formation of the M3b cluster, demonstrating that the individual binding constants of ... values of 0.0, 0.8, 1.6, 2.4, 3.2 and 4.0 of Cd2+ at 22 °C Inset: Plot of changes in CD intensity monitored at 223, 240, 250 and 263 nm as a function of molar equivalents of Cd2+ up to a maximum of ... show the formation of intermediate Cd1a, Cd2a and Cd3a species, with the Cd4a species forming only after > equivalents of Cd2+ have been titrated Addition of 4.0 equivalents is required for 100%...
... Madin–Darby canine kidney (MDCK) cells, a study of chimeric forms of DPP IV has shown that the luminal domain of DPP IV carries dominant apical sorting information while the short cytoplasmic tail ... targeting is beginning to be perceived For instance, one striking example of c-Src location inside one organelle has been reported for the inner membrane of mitochondria of osteoclasts [57] Moreover, ... concentration of the soluble form of DPP IV may result in an increased proteolysis of GLP-1 peptides and thus decreased insulin secretion [20] The insulin-dependent phosphorylation of DPP IV observed...
... spans of TatA and TatB Deletion of the TM spans of TatA or TatB leads to a loss of activity [31] but the important characteristics of these regions have not been probed We constructed a series of ... analyzed for export of TorA-GFP using the assay protocols detailed in Fig For clarity, mutations in TatA are shown underlined and in grey (B) Combinations of mutations in the amphipathic regions of ... proton motive force [23] On the basis of these studies, it has been proposed that binding of substrate to the TatBC subunits triggers the recruitment of the separate TatA complex to form an active...
... Amount of products formed (mol percentage) are shown conducted in the absence of Tween 80 Finally, in the presence of Tween 80, oxidation of a-keto diarylpropane (X) resulted in the formation of ... over 90% of the added diarylpropane I was oxidized in the presence of Tween 80, while in the absence of Tween 80, 24% of the diarylpropane I was oxidized In addition, while about 30% of the diarylpropane ... Oxidation of diarylpropane substrates by MnP Time courses for the oxidation of diarylpropanes I and VI by MnP, in the presence and absence of Tween 80, are shown in Fig After 12 h of incubation,...
... methylation of the C-terminal half of the a1(I) resulted in either reduced binding for a1(I) CB7, or a complete loss of the binding for a1(I) CB6 The variation of the binding ability for N-methylated ... were analyzed for purity by means of a quantitative Hyp assay [38], electrophoresis in denaturing conditions [34], N-terminal sequencing for some peptides and for conformation by means of CD spectroscopy ... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of...
... were subjected to one cycle at 95 °C for min; 40 cycles at 95 °C for 30 s, 60 °C for 60 s and 72 °C for 90 s; and a ®nal additional extension period at 72 °C for Ampli®ed DNA fragments were puri®ed ... activity of this mutant enzyme could not be restored by the addition of Tween 40 [39], it can be concluded that the 482 Gly residue is important for the activity of the enzyme, likely being one of ... different carB alleles This provides genetic evidences for the multimeric organization of the enzyme phytoene dehydrogenase in Phycomyces The nature of the mutations in the complementing carB alleles...
... the major circulating form of vitamin D, 25(OH)D, into the active hormonal form, 1,25(OH)2D So, there is a local mechanism in many tissues of the body for converting the form of vitamin D in the ... treated for an early stage of breast cancer have been randomized into two groups The dietary goals for the test group of the study are servings of vegetables, 16 oz of vegetable juice, servings of ... effect of fruits and vegetables was found in 128 of 156 studies that gave relative risks For most cancers, people in the lower quartile (1/4 of the population) who ate the least amount of fruits...
... providing kinetic evidencefor the direct complex formation of P450nor with NADH Materials and methods Mutagenesis and expression plasmids The construction of each mutant of P450nor of F oxysporum ... pocket of P450nor (Eur J Biochem 271) 2891 Fig Formation of the spectral intermediate (I ) observed upon + reduction of the Fe3 –NO complex of the D393A mutant with a higher concentration of NADH ... follows its formation, although the rate of decomposition is much slower than that of I formation (cf Figure 6) hydrogen bond network containing Asp393 is essential for the binding of NADH rather...
... 0.004 0.003 0.002 0.001 Cg-BMPR1 Cg-TGFβsfR2 0.04 number of copies of Cg-TGFβsfR2 per copy of GAPDH 0.25 number of copies of Cg-BMPR1 per copy of GAPDH 0.005 st ul a G as Tr tr ul oc a op ho re L ... 0.006 yt es 1.0 0.007 B la 1.2 O oc 1.4 number of copies of Cg-TGFβsfR2 per copy of GAPDH Cg-TGFβsfR2 O oc number of copies of Cg-BMPR1 per copy of GAPDH Cg-BMPR1 0.2 0.15 0.1 0.05 0.035 0.03 ... promiscuous receptor Evidencefor the conservation of the mechanism of mesoderm induction in molluscs and across bilaterians Although Lartillot and colleagues have observed for a number of developmental...
... wild type [16,17] The pKa for the alkaline transition being similar to that of the wild type, % 11.1 [18] Finally, comparison of the X-ray structuresof the oxidized forms of the native [5,19] and ... by the carbonyl oxygen of G168 Inclusion of F221 obscures the local structure in the proximity of T171 and for clarity is omitted Table Comparison of functional parameters for HRPC proximal pocket ... into the role of key catalytic residues [69] A signicant outcome of many of these studies [6,1012] has been the discovery of coupled effects that can be understood on the basis of a hydrogen...
... 14693–14698 Van Hoof, C., Ingels, F., Cayla, X., Stevens, I., Merlevede, W & Goris, J (1995) Molecular cloning and developmental regulation of expression of two isoforms of the catalytic subunit of protein ... orthologue of PR48 Moreover, analysis of the structure of the latter made it possible to improve the current knowledge of the mammalian PR48 cDNA To extend these findings, the genomic structuresof the ... templates for DNA sequencing 5¢-RACE technique 5¢-RACE reactions were performed on about 1.5 lg of total RNA of prophase X laevis oocytes with U of AMVreverse transcriptase (5¢/3¢RACE kit, Roche) For...
... of the DC-SIGN-related lectins for PA-glycans Fig S1 Schematic representation of 157 oligosaccharide structures used for FAC analysis Fig S2 Structural formulae of Man9GlcNAc2-MTX, Mana1-3Man-PA ... absence of DC-SIGN–Fc As shown in Fig 9B, the three major bands (i.e 1, of 160 kDa; 2, of 75 kDa; and 4, of 55 kDa) were present on a silver-stained gel, as well as an extra band (band 3, of 65 ... of 4b-phorbol 12-myristate 13-acetate (Sigma) After incubation for 96 h, cells were used for uptake and adhesion experiments To examine the surface expression of DC-SIGN on dTHP-1 cells and of...