establishment of respiratory air liquid interface cultures and their use in studying mucin production secretion and function
Indoor Air Pollution Associated with Household Fuel Use in India: An exposure assessment and modeling exercise in rural districts of Andhra Pradesh, India doc
... percent in sample against percent in sample In both samples, usage of improved stoves was negligible Kerosene stoves were used in 32 percent of households in sample and 26 percent of households in ... beyond their own health (most importantly, children’s health) Health risks from indoor air pollution in household settings thus have complex inter-linkages, and a holistic understanding of these linkages ... exposure to indoor air pollution and increased incidence of chronic bronchitis in women and acute respiratory infections (ARI) in children have been documented (Armstrong and Campbell 1991, Robin et...
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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx
... type A and B influenza viruses have genomes consisting of eight negative-sense single-stranded viral RNAs (vRNAs), each of which contains a coding region and terminal 5' and 3' noncoding regions ... promoters used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... production because of the capacity for high virus yields of both A and B strains in this cell line In addition to the cell substrate used for influenza virus production, response time to pandemic influenza...
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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf
... type A and B influenza viruses have genomes consisting of eight negative-sense single-stranded viral RNAs (vRNAs), each of which contains a coding region and terminal 5' and 3' noncoding regions ... promoters used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... production because of the capacity for high virus yields of both A and B strains in this cell line In addition to the cell substrate used for influenza virus production, response time to pandemic influenza...
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Báo cáo hóa học: " Drying nano particles solution on an oscillating tip at an air liquid interface: what we can learn, what we can do" pot
... tip and accumulate at the triple contact line of the meniscus Fig 10 A ring stain and a demonstration of the physical processes involved in production of such a stain production of such a stain ... processes may also happen giving a mixing of homogeneous covering and ring structures [21] Oscillating multi wall carbon tip at the air liquid interface : case of solution of nanoparticles coated ... the triple line is weakly pinned or not pinned at all When the triple line is pinned, at a constant evaporating flow, the thinning of the meniscus leads to a diverging liquid velocity inside the...
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Báo cáo y học: " Successful establishment of primary small airway cell cultures in human lung transplantation" doc
... collected into a tube containing ml of RPMI on ice and the brush tip was also cut off and collected after the final brushing After the completion of brushings, 20% (v/v) FCS was added to the tubes and ... confirming small airway lineage [25], and the utility of this in vitro model was limited since cultures were not expanded through repeated passage Expanded cultures facilitate varied experiments and ... contamination with passenger organisms, insufficient cell yield and BOS Bacterial and fungal contamination occurred despite the inclusion of antibiotic (gentamycin, penicillin and streptamycin) and...
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Báo cáo y học: "The establishment of a primary spine care practitioner and its benefits to health care reform in the United States" docx
... Judicious use of imaging and appropriate communication of findings may also help avoid the iatrogenic disability that can arise as a result of the medicalization of imaging findings that are of questionable ... keen understanding of the management of non-surgical SRDs [111] This is likely because the bulk of the training of these specialists is focused on the application of interventional and surgical ... trained primary spine care practitioners Other disciplines that include some level of spine care training within their respective curricula are institutions of osteopathic medicine and physical therapy...
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Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difficile pdf
... presence of saturating concentrations of the metal cofactor Together, these data are indicative of two modes of substrate binding (i.e open and closed) The closed, active form of UDP-Glc binding is ... hydrolysis of UDP-Glc in the absence of toxin Inhibition studies were performed by varying the MUG and UDP-Glc concentrations and following the kinetics of the hydrolysis reaction Synthesis of MUG, ... 7.6 and 150 mM KCl containing varying concentrations of UDP-Glc (0–145 mM) were studied The variable concentrations of UDP-Glc were obtained by starting with a stock solution containing all of...
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Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx
... membrane-binding domain of Era also required the GTP-binding and GTPase activities of the protein for function and whether, biochemically, the RNAand membrane-binding domains overlap To investigate ... 1–185), which contains the GTPase domain, and Era-C (corresponding to amino acids 141–299), which contains the RNA-binding domain (KH domain) and the region essential for the binding of Era to the ... whether the binding of Era to 16S rRNA requires the GTPase activity of the protein and whether the RNA-binding domain can bind RNA in the absence of the GTPase domain Structurally, it remained to...
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Báo cáo khoa hoc:" A review on SNP and other types of molecular markers and their use in animal genetics" ppt
... that interesting to use, due to the fact that they are of the dominant type, the RAPDs and AFLPs have a great advantage in terms of ease of use in the laboratory Indeed, fingerprint types of patterns ... to in Tables I and II are still in use and the choice of one or another can be guided by the variety of parameters indicated, mainly according to the goal of the study and the importance of the ... available, indications of high densities of SNPs in defined regions can be found A sequencing study of fragments of the leptin and amyloid precursor protein (APP) genes in 22 diverse individuals...
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Báo cáo y học: "valuation of endoscopic vein extraction on structural and functional viability of saphenous vein endothelium" potx
... (stimulatory/ functional response) of bradykinin are shown in Figure Bradykinin stimulation resulted in increase in calcium and nitric oxide fluorescence in both groups However, measurement of calcium ... in the process of evaluating the effects of CO2 on vein structure and function to eliminate one possibility Conclusion The principal findings of this investigation is that unlike in our previous ... during harvesting of SV using an endoscopic technique [28] The parameters of endothelial structure and function were altered significantly in the endoscopic samples, including the impairment of...
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Báo cáo y học: "Identification of an effective siRNA target site and functional regulatory elements, within the hepatitis B virus posttranscriptional regulatory element" ppt
... to function in the context of heterologous genes by enhancing expression of intronless transcripts of b-globin and c- myc [27,50-52] In this study, the PRE failed to increase activity of the intronless ... tract binding protein (PTB) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) [33-36] (Figure 1A) and binding site of T-cell intracellular antigen (TIA-1) [37], however, the core function of ... including a human La binding site [30,31], stem-loop structures, HBV SLa and HBV SLb [32], a cis-acting splicing regulatory element (SRE-1) [24], the binding sites (PRE III) of polypyrimidine tract...
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Báo cáo y học: " The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells" potx
... formation of reactive oxygen species that are directly implicated in the induction of cell injury via lipid peroxidation and expression of pro-inflammatory cytokines [6] In the liver, baseline metabolism ... Decline of LSECs cultures may be observed in dishes maintained at atmospheric oxygen levels (a-c) after several days of culture whereas the LSEC cultures maintained at 5% oxygen preserved intact ... fuctionally intact rat LSEC cultures during 48 h However, from the third day of incubation, the cultures gradually lost their integrity This loss was nevertheless prevented by incubation of cultures...
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The development of acidic protein aptamers using capillary electrophoresis methods and their use in surface plasmon resonance
... selection of aptamers with both fast and slow binding kinetics It also can be used to accurately determine the binding affinities, kinetics and specificity of aptamer sequences In my PhD, the use of ... 1-4 using both the NECEEM and fluorescence intensity methods 80 Table 4.4 Binding affinities of lysozyme, trypsinogen, chymotrypsinogen A and myoglobin using fluorescence intensity against ... the use aptamers as affinity probes in ACE include IgE, HIV type reverse transcriptase, thrombin and antithrombin III12-14 The use of aptamers in biosensors is becoming a huge area of interest in...
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Genetic study of hematopoiesis in zebrafish characterization of zebrafish udu mutant, positional cloning and functional study of udu gene
... activation of PU.1 target genes, whereas, PU.1 inhibits GATA-1 function by binding to the GATA-1 C-finger and inhibiting GATA-1 DNA binding (Nerlov et al., 2000; Zhang et al., 2000) In summary, combination ... within its Ets domain, PU.1 interacts with several factors, including GATA-1, GATA-2, Runx1, C/EBP (CCAAT enhancer binding protein) and C-Jun, and mediates both positive and negative regulation of ... 1994) Ikaros and its molecular partners, Helios and Aiolos, are zinc finger transcription factors, which have four N-terminal and two C-terminal zinc-finger domains The N-terminal domains are crucial...
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steinmetz cp complex-quantities-and-their-use-in-electrical-engineering
... 05,/ Case Non-inductive line of Non-inductive load of Case Non-inductive line of Inductive load of Case Inductive line of Inductive load of Case Inductive line of Non-inductive load of 1 — j — lj ... space, and of which the complex quantity in a plane is only a special case M B STKINIHTTZ: In introducing j , first as distinguishing index and then defining it as V — my object was to introduce ... phase of the main current and the E M F In consequence hereof, the current will change in the line •from point to point, in intensity as well as phase, and the E M ÆTHE ORCE RF I 58 8TEINMETZ...
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Land quality indicators and their use in sustainable agriculture and rural development pdf
... ; examples of improvements in land quality (e.g., results of soil conservation and land -use planning case studies); Land quality indicators and their use in sustainable agriculture and rural development ... issues and participatory approaches Land quality indicators and their use in sustainable agriculture and rural development 11 CONCEPTS, DEFINITIONS AND PRINCIPLES "Land", the "functions of land", ... is a kind of land use described or defined in a higher degree of detail than that of a major kind of land use (such as rainfed agriculture or forestry), as an abstraction of actual land -use systems...
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Báo cáo y học: "A standardized scoring method for the copy of cube test, developed to be suitable for use in psychiatric populations" pdf
... a novel and detailed standardized method of administration and scoring of the copy of the Necker cube test and to preliminarily test this method in schizophrenic patients This new scoring method ... of variance explained 29% 28% Total variance explained Closing -In Index (CiI): Close -In (CI) Explained variance 100.00 0.00 100.00 0.00 NS 57% Significant values are in bold Because of lack of ... Additionally, the means and the standard deviations of the differences concerning each SCCT item between rating and re-rating were calculated and the plots of the rating vs re-rating and difference vs average...
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Urban Air Pollution and Climate Change as Environmental Risk Factors of Respiratory Allergy: An Update potx
... Components of Air Pollution and Allergens in Inducing respiratory Allergy Air pollution may be responsible for the following: • Interaction with pollen grains, leading to increased release of allergens ... factors and asthma raises the question of how increasing levels of greenhouse gases and concomitant climate change in uence the frequency and severity of respiratory allergy Global warming induced ... pollution and climatic changes) and increased trend of respiratory allergy J Invest Allergol Clin Immunol 2000;10:33-9 Peden DB Air pollution: indoor and outdoor In Adkinson NF, Yunginger JW,...
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Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... http://www.medsci.org Int J Med Sci 2011, 41 Table Serum samples used in training and testing sets MALDI-TOF MS Samples Colorectal cancer Healthy volunteers Total Following binding and washing, the bound proteins ... Committee of Taizhou Municipal Hospital, and had the informed consent of the patients and volunteers The patients and serum samples were then divided into two groups: the ‘‘training’’ set and the blinded ... protein peaks in sera (showed in Table 3) To develop biomarker patterns for the diagnosis of CRC, the intensities of the protein peaks in the training set were submitted to BPS A total of four...
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Establishment of Effluent Standards for Industrial Wastewaters in Korea: Current Issues and Suggestions for Future Plan
... possible In this study, the approach in setting the effluent standards with a limited data set obtained so far according to the kind of pollutants and industries was investigated This paper focused ... current state of effluent standards in Korea and the considerations to be used in revising effluent standards and the framework referring to the cases of developed countries The purpose of this study ... Australian and New Zealand guidelines for fresh and marine water quality Australian and New Zealand Environment and Conservation Council and Agriculture and Resource Management Council of Australia and...
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