epigenetic and microrna regulation in stem cells

Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps

Ngày tải lên : 09/08/2014, 10:20

... temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma) for minutes and counterstained ... antibody (green), and 4',6-diamidino-2-phenylindole (blue) Results showed strong staining for CD13, CD29, CD44, CD90 and CD105, weak staining for 3G5, and negative staining for low-affinity nerve growth ... CD105 (markers for mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker...

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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation

Ngày tải lên : 10/09/2015, 09:08

... domains, which are highly conserved DNA-binding domains In recent years, Sox2 has become widely regarded as a reprogramming factor important for inducing and maintaining 15 pluripotency in ESCs ... the ongoing phase I/II trial in chronic thoracic spinal cord injury by StemCells, Inc involving the use of their proprietary human fetal-derived NSC line HuCNS-SC (A list of ongoing clinical trials ... Non-coding RNAs are gaining attention for their role in regulating a number of epigenetic events, such as gene imprinting and RNAi-mediated gene silencing (Grewal and Moazed, 2003; He and Hannon,...

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Báo cáo hóa học: "Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy" doc

Ngày tải lên : 18/06/2014, 16:20

... lower in group than in groups 3, 4, and 5, significantly lower in group than in groups and 5, and also notably lower in group than in Page 10 of 16 group This finding indicates that early combined ... stained cells in LV myocardium were significantly higher in group than in other groups, significantly higher in groups and than in groups and 5, and also notably higher in group than in group Lin ... groups and These findings indicate that the expression of cytochrome C, an index of energy supply and storage in mitochondria, was notably lower in group than in groups and The increase in cytosolic...

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Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc

Ngày tải lên : 18/06/2014, 16:20

... Medicine 2011, 9:12 http://www.translational-medicine.com/content/9/1/12 Figure Intraoperative view showing clinical application of stem cells into the failing heart with multiple targeted injections ... Conception and design, provision of patients, data analysis and interpretation, manuscript writing PA Conception and design, data analysis and interpretation, manuscript writing HA Data analysis and interpretation ... Conception and design, data analysis and interpretation, manuscript writing All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests...

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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot

Ngày tải lên : 09/08/2014, 01:22

... and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue explants...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and...

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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt

Ngày tải lên : 14/08/2014, 21:20

... of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin ... upon activation of the insulin and wingless pathways in S2 cells Binding of insulin-like peptides to the insulin receptor in Drosophila cells leads to the activation of dPI3 kinase, which Genome ... of the wingless pathway (Table 1) and changes in alternative splicing of several key genes involved in wingless signaling, including the wingless receptor frizzled2 (fz2) [57] and the wingless...

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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells

Ngày tải lên : 12/09/2015, 21:26

... musashi and prominin-1 (CD133) Nestin is an intermediate filament protein expressed in neuroepithelial cells (Lendahl et al., 1990) and is found mainly in proliferating cells of the CNS during early ... maintain these cells in an undifferentiated, quiescent state For stem cells to be activated, the niche environment must change (Fuchs, 2007) 1.4.3 Stem cells and cancer Stem cells and cancer cells ... behave in vitro and in vivo in terms of survival, proliferation and differentiation Introduction: 27 1.7 Methods to study neural stem cells 1.7.1 Identifying neural stem cells in vivo In vivo...

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Role of bcl 2 in metabolic and redox regulation via its effects on cytochrome c oxidase and mitochondrial functions in tumor cells

Ngày tải lên : 14/09/2015, 08:42

... pro-apoptotic activity of Bax and Bak by preventing the binding of these proteins to anti-apoptotic Bcl-2, suggesting that these pro-apoptotic proteins kill by binding and inhibiting their anti-apoptotic ... COX Va and COX Vb as cancer markers: 32 Interestingly, recent studies have demonstrated up -regulation and increased involvement of COX Va and Vb in a variety of cancers Autocrine gastrins-induced ... thereby limiting its influence on O2- levels in the treated CEM/Bcl-2 cells Indeed, if MnSOD was engaged in blunting mitochondrial O2- increase in antimycin-treated CEM/Bcl-2 cells due to increased...

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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx

Ngày tải lên : 19/02/2014, 00:20

... chemical inhibitor SB-431542 to block activin ⁄ TGF-b signaling in murine stem cell cultures Both experiments resulted in a strong growth inhibition of the stem cells but, fascinatingly, did not interfere ... BMP signaling in stem cells tolerate lack of ALK3 in absence of the inhibitor Although functional proof is missing, the authors found up -regulation of ALK1 and ALK2 in these cells and suggest ... BMP signaling is indeed an antiproliferative and differentiation inductive signal for neural stem cells, again (as shown and discussed for murine ES -cells) modulated by LIF ⁄ STAT3 in a highly...

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Báo cáo khoa học: Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex ppt

Ngày tải lên : 07/03/2014, 05:20

... examined the bovine sperm-binding activity of heterospeciﬁc bovine ⁄ porcine rZP3 ⁄ rZP4 mixtures Both porcine rZP3 ⁄ bovine rZP4 and bovine rZP3 ⁄ porcine rZP4 inhibited bovine sperm–ZP binding at a ... chains of mouse ZP in sperm binding [27–29] In pigs, neutral tri-antennary and tetra-antennary complex-type chains have the strongest sperm-binding activity of the N-linked chains of ZP [30], and ... glycopeptidase F, indicating that rZP2 and rZP4 have N-linked chains (Fig 7A), the resulting bands were not sufﬁciently resolved to deduce the number of N-linked chains in these proteins Native bovine ZP2...

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Báo cáo khoa học: Function of microRNA-375 and microRNA-124a in pancreas and brain doc

Ngày tải lên : 29/03/2014, 22:21

... cytoskeleton organization and regulate glucose-induced insulin secretion [65,66] These ﬁndings represent another interesting aspect of the action of myotrophin in cells and may explain the mechanism by ... role in the differentiation of progenitor cells to mature neurons by directly regulating polypyrimidine tract-binding protein 1, which is involved in alternative pre-mRNA splicing in nonneural cells ... Function of miR-375 and 124a in pancreas and brain code for proteins [1,2] A major advance in understanding the regulation of genetic information came with the discovery of microRNA (miRNA) molecules...

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Báo cáo khoa học: Impact of cyclic hypoxia on HIF-1a regulation in endothelial cells – new insights for anti-tumor treatments doc

Ngày tải lên : 30/03/2014, 02:20

... required in the use of NOS inhibitors as single anti-tumor treatments More generally, by providing new insights into the regulation of HIF-1a in the context of tumor O2 ﬂuctuations, this study integrates ... corresponding O2 consumption rate (lMÆmin)1) as observed in Fig 4A (left y axis) and the corresponding HIF-1a expression values (right y axis) determined as in Fig 1; note the parallel increases in ... was added to cells, which were then drawn into glass capillary tubes They were then rapidly placed into quartz electron spin resonance tubes and maintained at 37 °C during recording on a Bruker...

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Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx

Ngày tải lên : 30/03/2014, 10:20

... K Rathinasamy and D Panda [10] The binding site of benomyl in tubulin is yet to be determined However, it has been suggested that benomyl binds to tubulin at a site that is distinct from ... tubulin at a site that is distinct from the colchicine and vinbastine binding sites in tubulin [10,11] Although benomyl inhibits mitosis in mammalian cells, its antimitotic mechanism of action is ... the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is required for inactivating the spindle checkpoint signal and progression in the cell cycle [20] The...

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Báo cáo lâm nghiệp: "Influence of individual tree and stand attributes in stem straightness in Pinus pinaster Ait. stands" pps

Ngày tải lên : 08/08/2014, 01:22

... being an own character more outstanding than stem lean In this way, the stem straightness in mature stands was determined by the stem sinuosity classification [15]: SC1: Straight SC2: Light sinuosity ... distribution and it is often used in reforestation Twenty provenance regions of P pinaster have been defined in Spain [4] Pinus pinaster shows a general tendency for sinuosity in comparison with other Pinus ... to describe stem form in mature stands Galera et al [15] proposed one classification for stem sinuosity and another one for stem lean In “Sierra de Oña” provenance stem sinuosity in mature trees...

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Báo cáo khoa học: "Spatial and seasonal variations in stem respiration of beech trees (Fagus sylvatica)" potx

Ngày tải lên : 08/08/2014, 14:20

... differences in growth respiration (2) The distribution of living cells within the stem can affect maintenance respiration rates [45, 46] (3) The transport and storage of carbohydrates in the stem and in ... living cells in the periderm were integrated over the cross-sectional area of the stem RESULTS 3.1 Stem growth Stem diameter increment in the crown started at the end of April in 1997 [22] and ... Spatial variation in stem respiration and Q10 in the cuvettes during the season 1998 at breast height, mid -stem and for the upper stem of dominant beech trees and at three positions within the crown...

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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx

Ngày tải lên : 09/08/2014, 07:20

... tissues were finely minced and digested with 0.2% collagenase in DMEM containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin (complete DMEM) Following overnight incubation ... shown) Inversely, the mean induction of osteocalcin and alkaline phosphatase was statistically higher in cells from BM than in cells from the synovium In these conditions, the primary skin fibroblastic ... environment inside the joint favoring the inhibition of immune cell proliferation In the inflammatory context, where TNF-α and IFN-γ are prominent, induction of proinflammatory cytokines may reverse...

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Báo cáo y học: " Basal core promoters control the equilibrium between negative cofactor 2 and preinitiation complexes in human cells" pdf

Ngày tải lên : 09/08/2014, 20:21

... formation and TFIIB binding in vivo The high prevalence for the occurrence of motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and ... (black line) and replicate (grey line) relative to aligned TSSs at 10-bp resolution Binding of TBP and the initiating form of RNAPII (phosphorylated at serine in its carboxy-terminal domain) were ... one initial cycle consisting of minutes at 55° (during which polymerase was added), minutes at 72°C and minutes at 94°C, followed by 22 cycles of 0.5 minutes at 94°C, 0.5 minutes at 60°C and minutes...

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Báo cáo y học: "Modeling antibiotic and cytotoxic effects of the dimeric isoquinoline IQ-143 on metabolism and its regulation in Staphylococcus aureu" pot

Ngày tải lên : 09/08/2014, 22:24

... (reactions in Tables S2 and S3 in Additional file 1) of metabolism in staphylococci systematically included all pathways for which gene expression data pointed to major changes (Tables and 2) in individual ... baculovirus-infected insect cells were incubated with the corresponding substrates and IQ-143 at concentrations of μM, 10 μM, and 100 μM as described by Unger and Frank [34] As shown here (and in Figure ... assays, and was involved in data analysis CL was involved in programming tasks (PERL/R) and JB in database management (Protecs) KO did all gene expression analysis experiments and provided infection...

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Báo cáo y học: "Nipah virus infection and glycoprotein targeting in endothelial cells" ppsx

Ngày tải lên : 12/08/2014, 02:20

... in the F protein led to an apical redistribution in endothelial cells whereas only tyrosine 525 is involved in targeting in epithelial cells Neither the di-tyrosine nor the di-leucine motif in ... F and G proteins were separated by SDS-PAGE and blotted to Table Summary and comparison of NiV infection and glycoprotein targeting in polarized epithelial and endothelial cells Epithelial cells ... basolateral sorting motifs, a di-tyrosine motif in the F protein (Y542/ 543) and a di-leucine motif in the G protein (L41/42) had no influence on basolateral sorting (table 1) Epithelial and endothelial...

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