enzymes involved in the release of flavor compounds from glycosides

Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt

Ngày tải lên : 30/03/2014, 13:20

... [23] In the present study we have cloned the murine genes coding for the enzymes involved in the salvage pathway of GDP-L-fucose L-fucokinase and GDP-L-fucose pyrophosphorylase were expressed in ... variants of the murine fucokinase genes were expressed in COS-7 cells in frame with a 10-amino acid E2Tag present in the pQM vector The molecular masses of fucokinase proteins were determined by ... amino acid sequence of mouse L-fucokinase The predicted amino acid sequence for the coding area of the long splice variant of fucokinase consists of 1090 amino acids Due to alternative splicing,...

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Risks Involved in the Use of Herbal Products

Ngày tải lên : 25/10/2013, 05:20

... result from their ability to inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting ... aspirin or other NSAIDs may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify ... kava-containing products from the market Based on these and other reports in the United States, the Food and Drug Administration (FDA) issued a consumer advisory in March of 2002 regarding the “rare”...

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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

Ngày tải lên : 20/02/2014, 11:20

... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... ThDP-dependent enzymes Substrate binding site and catalytic residues The active site cavity in IPDC extends from the thiazolium ring of the cofactor to the surface of the protein The entrance of the active ... structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ThDP binding sites are located in narrow clefts at the interfaces...

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Tài liệu Báo cáo Y học: Identiﬁcation of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf

Ngày tải lên : 21/02/2014, 01:21

... implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... implicated in the biosynthesis of the aminonucleoside moiety of puromycin [6–8] Interestingly, their organization is identical to that of the pur cluster, with the exception of pur6, which is absent from ... [33,34] In contrast, its similarity to type III PKSs, which lack this domain, is scant This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetase domain of the PKSs...

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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

Ngày tải lên : 22/02/2014, 07:20

... listed in Table that most, if not all, of the amylose is synthesized in vivo when the rate of amylopectin synthesis becomes minimal At these moments the rates of amylopectin synthesis are only 5% of ... rate of amylopectin synthesis The increase in crystallinity witnessed during these ﬁrst 12 h is in line with the high rates observed for amylopectin synthesis However between 12 and 58 h the rate ... amylose continues to accumulate at the ﬁnal stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]) In vitro synthesis of amylose Because in Chlamydomonas...

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Báo cáo khoa học: Identiﬁcation of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt

Ngày tải lên : 08/03/2014, 10:20

... GalNAc-T1, might be involved in the catalytic function of the enzyme To investigate whether the cysteine residues modiﬁed by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 ... University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA pDN42 was ... deleted from the rat isozyme, and an insulin signal sequence and a Protein A-IgG binding domain were fused to the resulting N terminus of the sequence The recombinant truncated GalNAc-T1 was then...

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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc

Ngày tải lên : 16/03/2014, 22:20

... treatment The high degree of protection by ADP suggests that the mitochondrial injury is caused by opening of the mPTP There is evidence that ADP inhibits opening of the mPTP by occupying binding sites ... the induction of oxidative stress during ischemia ⁄ reperfusion in brain There is a growing body of information concerning the mechanism of ROS generation by the respiratory chain in mitochondria ... during H ⁄ R performed in the presence or absence of 3.5 lm Ca2+ The mitochondrial incubations were carried out in the cuvette of the luminescence spectrophotometer at 30 °C in the presence of...

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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot

Ngày tải lên : 16/03/2014, 23:20

... success in expressing a recombinant XDH in this organism Expression of XDH requires the incorporation of the MPT center, placement of the terminal sulfur ligand on the Mo, and the incorporation of ... reduce the level of cysteine and increase the level of cystine in the cell, thus reducing the availability of a sulfur donor for the sulfuration reaction producing active XDH This would explain the ... stoichiometric and independent of the presence of xdhC, and their data suggest that the level of MPT sulfuration is lower in the absence of xdhC than in its presence Assuming that the level of incorporated...

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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf

Ngày tải lên : 17/03/2014, 10:20

... that of the background The binding site was found to be located at the C-terminus of the protein although binding of the C-terminus half (CH) is only half of the full-length protein (Fig 3) Interestingly, ... only one, the atpF intron, while CRS2 is involved in the splicing of nine of the 10 chloroplast group IIB introns [6,15,37] The expression of CRS2 in E coli together with the corresponding RNA ... (35) is indicated promote the splicing A possible model of how HCF152 is involved in the stabilization of the splicing products of the petB intron could be that the protein binds to the UTR region...

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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf

Ngày tải lên : 22/03/2014, 21:20

... the synthesis of amylopectin, it has been suggested that SSI is mainly involved in the synthesis of small chains of this fraction Furthermore, SSIV has been found recently to be involved in the ... interaction between the N- and C-terminal domains of SSIII in vitro Mapping of the CD-binding region in the N-terminal SBDs In order to identify the SBD region required for the SBD–CD interaction, we ... in the D2 domain and D(495–535) in the D3 domain This result is in agreement with our previous studies using truncated SSIII isoforms, showing the importance of the D23 domain in starch binding...

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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf

Ngày tải lên : 23/03/2014, 03:20

... obtained from the patient The COS-7 and 293T cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) Isolation and induction of pre-adipocytes Pieces of adipose ... analyses of the relative levels of the indicated proteins after western blotting (as in D) were carried out Similar experiments were performed ﬁve times and the intensity of the individual bands in ... subcellular compartments other than lipid droplets The expression of CIDEC is elevated during the differentiation of adipocytes To gain further insight into the roles of CIDEC in adipocyte differentiation,...

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Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

Ngày tải lên : 23/03/2014, 04:20

... whether Synoviolin is involved in the degradation of PS cofactors using synoviolin-null cells, as PS cofactors undergo the ubiquitin ⁄ proteasome pathway We report that Synoviolin is involved in ... polyglutamine-expanded huntingtin [18], the tumor suppressor Synoviolin is involved in the degradation of nicastrin gene p53 [19] and Parkin-associated endothelin receptor-like receptor [20] In this ... regulates the trafﬁcking of NCT within the Golgi compartment, because the maturation of the sugar modiﬁcation of the protein occurs within the Golgi compartment Recently, there has been an expansion of...

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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identiﬁed substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf

Ngày tải lên : 29/03/2014, 21:20

... are involved in the transmission of mechanical signals to signaling pathways In the M line, mechanical stimulation activates the interaction of a protein complex with the kinase domain of titin, ... pathway and an increase in muscle ﬁber apoptosis [8] The participation of NF-jB signaling in the pathogenesis of LGMD2A is therefore an interesting possibility The ﬁndings obtained in the present ... the binding to titin by improving the accessibility of the titin-binding domain Interestingly, the importance of CARP interaction for its function was recently demonstrated in a FEBS Journal...

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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

Ngày tải lên : 30/03/2014, 04:20

... residues were individually mutated (C) The spacial proximity of active site residues in the L-domain and connecting region II which are potentially involved in the binding of D-glucose in the super-open ... E290) and the S-domain (residues T168 and K169) (Fig 1B) To identify the contact residues involved in the initial binding of Glc to the super-open state (Fig 1C) of this two domain hinge-bending enzyme, ... demonstrating that the binding of Glc at the active site indeed induces a large-scale domain movement that closes the active site cleft and creates the stereochemical environment for binding of the...

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Báo cáo khoa học: Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide ppt

Ngày tải lên : 30/03/2014, 08:20

... Lgt4 Results In order to investigate the function of the glycosyltransferase enzymes involved in the biosynthesis of LOS of M catarrhalis, and to gain some insights into the mechanism of serotype ... irradiating each of the anomeric signals in turn to obtain the H chemicals shifts of the corresponding remaining ring protons Again, assignment of the Kdo residue was possible from examination of ... GlcNAc being retained at the terminal position of the (1 ﬁ 4) chain Additionally, the MS sugar analysis clearly indicated the absence of the GlcNAc residue Instead, in the absence of Lgt4 and Lgt5,...

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Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx

Ngày tải lên : 30/03/2014, 09:20

... Garmany) The ﬂow rate of the scintillation cocktail was 0.5–2 mLÆmin)1 Extraction of archaeol from H salinalium H salinarum was cultured in L of culture medium, containing g of casamino acids, g of ... for the enzyme [21] These facts strongly suggest the involvement of the enzyme, GGR, in the biosynthesis of membrane lipids in the hyperthermophilic archaeon A fulgidus, as predicted in the previous ... synthase from the thermoacidophilic archaeon Sulfolobus solfataricus Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked...

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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx

Ngày tải lên : 31/03/2014, 21:21

... of octanol) In respect of aliphatic ester production, it was found that the longer the carbon chain of the alcohol, the higher the AAT activity The activity of ester formation was in increasing ... provided by the manufacturer (Invitrogen) The strain harbouring the correct construction was incubated in selecting liquid medium according to Invitrogen recommendations, until the D600 of the culture ... [23,24] The production of recombinant proteins was then attempted in yeast In that case, the CM-AAT1-transformed yeast in culture evolved, in the absence of any exogenous precursor, a strong aroma of...

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báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf

Ngày tải lên : 19/06/2014, 22:20

... across the BBB to the sites of axonal injury in the brain [33,37] Both in vitro and in vivo findings suggest that hypoxia/ischemia-induced infiltration of monocytes and macrophages contributes to the ... of inflammatory cytokines and chemokines during hypoxia; whereas NFκB is mainly involved in transcriptional regulation of these genes during the phase of reoxygenation [8,40] The temporal interplay ... cells in an in vitro model of brain inflammation [43] Tuning of MCP-1 levels at different stages of the inflammation associated with ischemic brain damage may maximize the benefit effects of the inflammation...

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báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin" ppt

Ngày tải lên : 19/06/2014, 22:20

... down-regulation of NgR1 in matDCs promotes their adhesion to myelin, resulting in the selective accumulation of matDCs rather than immDC in the myelin debris-containing lesion This would result in further ... The interaction of DCs with myelin provides insight into how DCs act when in the presence of CNS myelin, such as during neurodegeneration and/or neuroinflammation The down-regulation of myelin-associated ... This could indicate a possible role for NgR1/2 outside the CNS, perhaps in the activation of DCs or in homing of DCs to specific tissues This is supported by the findings of the non-myelin associated...

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Báo cáo y học: "Inhibition of antithrombin by hyaluronic acid may be involved in the pathogenesis of rheumatoid arthritis" potx

Ngày tải lên : 09/08/2014, 06:22

... proteinase binding loop on the protein surface [19,20] Because the molecular structure of HA is analogous to that of heparin, HA might exert its effect by binding to the heparin-binding region of ... antithrombin However, such binding did not stimulate the activity of antithrombin as did heparin and heparan; in fact, it blocked the ability of antithrombin to inhibit thrombin In the present study, the ... effect of heparin on antithrombin was considerably decreased in the presence of HA, supporting the notion that HA could compete with heparin for the heparin-binding region of antithrombin Remarkably,...

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