enzymes involved in dna replication
Crystallographic studies on geminin CDT1 complex, proteins involved in DNA replication
... points 23 FIGURE 2.1 DNA replication licensing control by Geminin and CDKs 42 during the cell cycle FIGURE 2.2 Geminin Binding Domain of Human Cdt1 and Cdt1 44 Binding Domain of Human Geminin ... CDKs Geminin (Licensing) Figure2.1 DNA replication licensing control by Geminin and CDKs during the cell cycle In metazoa, Geminin, present from the onset of S phase to the end of M phase, binds ... information will prove useful in elucidating how Cdt1 and Geminin interact at the protein level We identified, cloned and expressed (in bacterial cells) the geminin-binding domain of human Cdt1 and purified...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... (S)-nandinine from (S)-scoulerine in vitro; indeed, CYP719A3 converted (S)-scoulerine to (S)-nandinine with high affinity (Km ¼ 0.54 lm) (Fig 6) Previous reports have shown that (S)-stylopine is ... 27 28 29 30 31 32 nine : 3¢-hydroxy-N-methylcoclaurine 4¢-O-methyltransferase involved in isoquinoline alkaloid biosynthesis in Coptis japonica J Biol Chem 275, 23398–23405 Inui T, Tamura K, Fujii...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... 23b, and exon 24 In conclusion, the alternative splicing maintains the reading frame along the entire protein, therefore the protein variants are identical in the amino-terminal end up to the ... study we have cloned the murine genes coding for the enzymes involved in the salvage pathway of GDP-L-fucose L-fucokinase and GDP-L-fucose pyrophosphorylase were expressed in COS-7 cells, and the ... parameters, including the Kozak translation initiation signal, as implemented in the GENSCAN analysis tool [28] Expression of fucokinase in mammalian cells The two splice variants of the murine fucokinase...
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Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... that is mostly involved in DNA binding but also in protein–protein interactions [17] Originally identified as being present in different transcription factors, it is now known that zinc finger structures ... present not just in transcription factors, but in basically all families of proteins involved in maintaining genomic stability, including DNA repair proteins and cell cycle control proteins [18] This ... folding of the minimal DNAbinding domain [50]; substitution of any of its zinccomplexing cysteines (Cys105, Cys108, Cys126 and Cys129) leads to diminished DNA binding and a severe reduction in...
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Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf
... domains of both proteins are shown in cyan with ADP indicated in red The helix-turn-helix (HTH)-containing DNA binding domain of DnaA is in green and the winged helix (WH)-containing domain of ... Cdc6 proteins [60,61] As can be seen in Fig 4, both proteins possess N-terminal AAA+ domains and C-terminal DNA binding domains (DBDs) In DnaA, the DBD contains a helix-turn-helix; in the archaeal ... combination of DNA bending induced by DnaA and the cooperative interactions between DnaA monomers on DNA result in local topological tension that manifests itself by unwinding of this intrinsically...
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Báo cáo khoa học: Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals pdf
... the importin b binding (IBB) domain in its N-terminus and the C-terminal NLSbinding domain In the absence of importin b, an auto-inhibiting part of the IBB domain forms an intramolecular interaction ... of basic amino acid residues such as lysine (K) and arginine (R), and (ii) the presence of conserved proline(s) (P) potentially involved in breaking secondary structural elements within the NLS ... NLS-binding domain, preventing the association with NLS on the cargo protein Thus, the presence or absence of importin b regulates the NLS binding ability of importin a The relatively large NLS-binding...
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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... absolutely inactive in initiating SV40 DNA replication in an assay consisting of purified enzymes [26,27] We then reproduced the absence of JCV species specificity using the monopolymerase DNA replication ... facilitate initiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting in origin unwinding under conditions of altered template DNA superhelicity ... SV40 TAg and DNA with the SV40 replication origin (A); the JCV system contains JCV TAg and DNA with the JCV replication origin (B) DNA polymerase (0.25 and 0.5 U) of the indicated DNA polymerase...
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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot
... intrinsic relationship between the RNA binding and protein folding in RNAUDE Interestingly, treatment of RNAUDE with RNase A did not result in signicant changes of the melting curve, indicating ... whereas DNA binding did not induce any protection (Fig 6B) These results suggest partially, but not fully, overlapping sites for RNA and DNA binding in UDE protein Furthermore, addition of DNA in ... RNA binding on protein structure and substrate-binding ability Proteins that bind and process nucleic acids are often capable of binding to both RNA and singlestranded or double-stranded DNA, ...
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Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
... are involved in fumarate activation (in yellow), CoA activation (underlined) and malate inhibition (in green) of the forward reaction are highlighted In addition, the regions involved in succinate ... regions involved in the differential regulation by fumarate and succinate could not be mapped by this approach Moreover, the mutation of R115 in NADPME2 indicated that this amino acid residue is involved ... at least in triplicate, and error bars indicate SD Significant inhibition (as indicated in Materials and methods): dark grey and singlehatched bars Significant activation (as indicated in Materials...
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Báo cáo Y học: The N-terminus of m5C-DNA methyltransferase Msp I is involved in its topoisomerase activity docx
... multiple Zn-binding domains at the N terminus of human DNA- (cytosine-5) methyltransferase: modulating the property of a DNA- binding domain by contiguous Zn-binding motifs J Mol Biol 257, 935–948 15 ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 25 Jost, J.P & Saluz, H.P (1991) A Laboratory Guide to in Vitro Studies of Protein DNA Interactions Birkhauser-Verlag, ... operator in the expression vector pMSP [22] E coli strain ER 1727 harboring recombinant plasmid pMSP was cultivated in Luria broth containing 150 lgÆmL)1 ampicillin at 37 °C M.MspI was purified using...
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Báo cáo hóa học: " Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a " pptx
... sites of DNA breaks [13,14] The process of PIR involves trimming of 5'-viral DNA ends prior to joining of viral and host DNA ends An intriguing role for MRE11 in the MRN complex would be trimming ... processing Gap filling Ligation Chromatin remodeling INTEGRATED RETROVIRAL DNA (REPAIRED) Figure Model for the role of NBS1 in post-integration repair Model for the role of NBS1 in post-integration ... 2008, 5:11 DNA produces an intermediate, in which the proviral DNA is flanked by short, single-stranded gaps in the hostcell DNA PIR completes integration through four distinct steps: trimming the...
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Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... be within the interior region of a co-infecting T4 DNA molecule, since T4 DNA is also circularly permuted In this way, the process of RDR can in principle initiate soon after an origin-initiated ... recombination proteins in the replication of damaged DNA [1-4] One major set of pathways involves the repair of DSBs and broken replication forks In addition, recombination proteins are involved in multiple ... proteins to initiate DNA synthesis, and serve as key sites for the regulation of replication timing While the actual determinants of origin activity remain ill defined in many systems, all origins...
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Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... helicase loading protein, the gp61 primase, and the gp32 single stranded DNA binding protein A.) the gp32 single-stranded DNA binding protein binds to regions of displaced DNA near the replication ... gp32 single-stranded DNA binding protein (gi:5354247, NP_049854) is a 301 residue protein consisting of three domain The Nterminal basic B-domain (residues - 21) is involved in cooperative interactions, ... backbone to increase stability of the interaction Cooperative binding of ssb proteins assists in unwinding the DNA duplex at replication forks, recombination intermediates, and origins of replication...
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báo cáo khoa học: " DNA polymorphisms and haplotype patterns of transcription factors involved in barley endosperm development are associated with key agronomic traits" pot
... conserved cis-acting element, which contains two distinct protein binding sites: the prolamin-box (PB) and the GCN4-like motif (GLM) Four transcription factors (TFs) are the gibberellin-regulated ... (GAMYB), the barley leucine zippers and (BLZ1, BLZ2), and the barley prolamin box binding factor Page of 11 (BPBF) that were shown to be involved in the transcription of B-hordeins encoded by the ... are given in additional file PCR for single nucleotide polymorphism (SNP) analysis by DNA sequencing was performed as described in full detail by [22] In preparation for DNA sequencing, we purified...
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Báo cáo y học: "APOBEC3G induces a hypermutation gradient: purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate in cellular viral RNA, and low in virion RNA" pps
... the domains of Vif that are involved in binding to A3G and A3F [12-17] Furthermore, as a proof of principle, work by Mehle et al has shown that Vif peptides overlapping the A3G-binding domain were ... on the ongoing replication of HIV-1 has not been studied in depth Recently, Mulder et al have shown that a replication- competent virus containing mutations in Vif residues involved in interactions ... mutations being the highest in viral DNA, intermediate in cRNA, and lowest in vRNA We also determined the frequency of G-to-A mutations present in vRNA obtained from HIV WT virus infections We...
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Báo cáo y học: "Genomic context analysis in Archaea suggests previously unrecognized links between DNA replication and translation" potx
... primase DnaG may be involved in DNA replication/ repair in Archaea since the gene encoding DnaG is adjacent to the gene encoding PolB3 in the three crenarchaeal lineages investigated and is located in ... Two main hypotheses can be put forward to explain the genomic association of genes encoding proteins involved in DNA replication and genes coding for proteins involved in translation First, replication ... oligonucleotide/oligosaccharide-binding (OB)-fold containing protein, a recently described new GTPase, DnaG) are encoded by genes that co-localize with DNA replication genes, suggesting that they may be involved in DNA transaction...
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Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
... light of the dyes increasing the chance of oxidation and / or bleaching The main contributing factors identified in this study are in agreement with a number of studies involving cDNA derived from ... accommodated in the VG interaction, but rather in the residual error The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions for S1 (7.8 %) and sampling differences ... variance in the VG interaction remaining virtually unchanged By combining S2 and S3, the Gene × Array interactions occurring specifically on S2 and S3 are pooled They are, thus, not accommodated in...
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Single DNA studies of architectural proteins involved in bacterial pathogenesis and meiosis in saccharomyces cerevisiae
... protein molecules and their interactions with DNA in real-time reactions and the dynamics of their DNA- binding Therefore, my objective of finding the characteristics of protein binding to single DNA ... could not fold DNA, instead, it made DNA more rigid (53) These contradicting results were reconciled by a later finding that H-NS in fact has two distinct DNA binding modes; a DNAstiffening mode due ... they found H-NS can fold DNA into hairpin structures (52) Based on this finding, H-NS was considered as a DNA- bridging protein However, in a singleDNA stretching experiment in 2003, another group...
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Regulation of spindle behaviour by DNA replication and damage checkpoints in budding yeast
... which recognize and bind to replication origins only during stages of low Cyclin-Cdc28 activity (i.e during G1), and permitting the initiation of replication only during high Cyclin-Cdc28 activity ... mechanism elucidated in the context of DNA damage checkpoint is responsible for preventing untimely spindle extension during vii replication checkpoint-induced S phase arrest We find that although ... Regulation of Spindle Dynamics by DNA Replication Checkpoint 183 6.2 Regulation of Spindle Dynamics by the DNA Damage Checkpoint 186 6.3 A Unified View of DNA Replication and Damage Checkpoints ...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray ... ethanol-induced expression and activation of cytokines and chemokines in monocytes and macrophages (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the upper gastrointestinal ... Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived...
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