enzyme binding to soil humus

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

Ngày tải lên : 16/02/2014, 09:20

... substrate binding Comparison with the structures of the related enzymes AppA and G1P of E coli suggests the existence of a common ancestor (‘prototype’) of HAPs, endowed with the potential to develop ... response to selective pressures arising from individual environmental conditions According to the crystal structures reported here, PhyK seems to have a preformed substrate -binding site and to be ... was added to PhyK in order to facilitate His-tag afﬁnity puriﬁcation The three distinct groups of HAPs are adapted to different habitats To support plant growth, bacteria not need to release...

13
766
0

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Ngày tải lên : 18/02/2014, 16:20

... regulatory point of view dCMP deaminase is activated by dCTP and inhibited by dTTP and both nucleotides act on the enzyme by binding to an allosteric site to alter the cooperativity of dCMP binding ... property of the enzyme to exist in two conformations and that dTTP stabilizes the inactive form by binding to the active site In this way, dCTP deaminase can use one nucleotide -binding site to gain a ... to the presence of a given concentration of inhibitor I and I0.5 is the concentration of inhibitor for half-maximal inhibition Equation (3) was used for hyperbolic binding of ligands to the enzyme...

11
577
0

Tài liệu Báo cáo khoa học: Afﬁnity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx

Ngày tải lên : 14/02/2014, 14:20

... that the observed heterogeneity in binding of [125I]TC-PCSK9 to HepG2 cells is attributable to binding to different populations of LDLR that exist prior to ligand binding (see below) Previous Biacore ... of the receptor via a two-step reaction in which the ﬁrst binding step, representing binding to the EGF-A domain, is followed by binding of PCSK9 to a second site within the receptor In this ... represent binding of PCSK9 to dimeric receptors that release bound ligand slowly because they convert rapidly The slow phase of association could represent binding to monomeric receptors that...

13
712
0

Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx

Ngày tải lên : 18/02/2014, 16:20

... terbium binding to this crystallin by ITC and determined the binding constant for the calcium mimic probe Terbium is believed to bind strongly to calcium -binding sites of proteins compared to calcium ... calcium -binding members of the bc-crystallin superfamily [14,15,20] Based on our results, together with the published data on calcium -binding to a few other members, we suggest that calcium -binding ... for further calcium -binding studies, otherwise the proteins were stored frozen at )80 °C Calcium -binding to bB2- and bA3-crystallins Because there is no known motif for calcium -binding in bB2- and...

13
449
0

Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx

Ngày tải lên : 18/02/2014, 16:20

... phosphorylation [33] Other cytoplasmic domains related to multichain immune recognition receptors were found to be intrinsically disordered even when bound to lipids [36] A role of the cytoplasmic tail of ... from 355 to 345 nm (Fig 5) Altogether, ﬂuorescence data conﬁrm binding of J1_tmic to SDS micelles and DMPG phospholipid vesicles, with at least partial embedding of one or both the tryptophan residues ... function of their cytoplasmic tails and, to our knowledge, only few examples have been reported [32,33] The cytoplasmic tail of the T-cell receptor f-chain [34,35] binds to lipid membranes through...

12
502
0

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

Ngày tải lên : 19/02/2014, 00:20

... explained as being due to exciton splitting between the symmetric pairs of [Cd(Scys)4]2 groups in the Cd4(Scys)11 binding site [27] As noncooperative metal binding is predicted to result in the formation ... a domain prior to that of the b domain Although the metal -binding reaction has been shown to proceed noncooperatively, this does not mean that a distinct order of metal binding to each of the ... view of metal binding to MT and have been cited regularly in recent reports The data presented in these papers clearly show domain-speciﬁc binding for M2+ (M ¼ Zn, Cd) initially to the a domain,...

9
533
0

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

Ngày tải lên : 19/02/2014, 05:20

... buffer, and n is the net number of protons transferred during binding To investigate whether there is a net proton transfer on IL-8 binding to the receptor N-domain, ITC measurements were carried ... IL-8 binding to its receptor CXCR1 N-domain has been characterized using ITC This report describes how different enthalpic and entropic factors could mediate chemokine binding to its receptor ... in binding may be due to binding of N-loop residues to the receptor N-domain The structure of IL-8 is known, and the structural basis for its function has been well studied [8–17] The receptor...

11
549
0

Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

Ngày tải lên : 19/02/2014, 12:20

... contribute to high-affinity binding of the ephrin B cytoplasmic domain to the Grb4 adaptor protein [11] The equivalent Tyr304 residue of Xenopus ephrin B1 was shown to be essential for binding to Grb4 ... peptide are generated to determine the binding specificity of the Grb4 SH2 in relation to the closest consensus binding sequence of the Src SH2 domain (i.e pYEEI) responded to the addition of the ... essentially the same binding affinities to the GST-PDZ protein in agreement with previous observations [8] Binding experiments with the isolated PDZ domain showed similar binding affinities to the ephrin...

12
551
0

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

Ngày tải lên : 19/02/2014, 16:20

... concentrated stock solution (% 30 mgÆmL)1) using a Hamilton (Reno, NV, USA) analytical micro syringe An equal volume of the protein was added to the reference cell, to correct for any contribution to the ... indicate that binding of these porphyrins to MCL is governed by enthalpic forces and that the entropic contribution to the binding process is negative The enthalpy and entropy of binding for the ... observed when the same porphyrin binds to jacalin [52] The CD studies presented here suggest that porphyrin binding to MCL is probably similar to porphyrin binding by ConA, and most likely involves...

9
646
1

Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt

Ngày tải lên : 19/02/2014, 16:20

... bottom of the autoradiograph (20 pM) was incubated without (–) (lane 1) and with 10 pM Hfq-His6 (+) (lanes 2–7) and increasing amounts of the competitor RNA indicated at the top of the autoradiograph; ... FEBS M Folichon et al A Hfq binding to RNA stimulates elongation by PAP I B Fig A monophosphate 5¢ end stimulates polyadenylation but not Hfq binding (A) Hfq binding to mono- and tri-phosphorylated ... terminator The PCR fragment digested by NdeI and BamHI was inserted into pUC18 vector that had been digested with the same enzymes The Hfq containing DNA fragment was excised with the same enzymes...

10
488
0

Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

Ngày tải lên : 20/02/2014, 02:22

... constants K MS1 to K MS5 have been introduced in the model to take into account subtraction of myristate by additional binding sites no to essentially uncoupled to FA1 and ⁄ or FAx Fatty acid binding ... 4672–4683 ª 2005 FEBS Myristate and Mn(III)heme binding to HSA–heme mation from the N to the B state) is very similar to that induced by myristate binding to site FAx Indeed, the HSA afﬁnity for Mn(III)heme ... established by FA binding to domain III (i.e to FA4 and FA5) [26,37–39] On the other hand, myristate bound at the limit of subdomain IA (i.e to FA2) was suggested to be functionally linked to Sudlow’s...

12
515
0

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

Ngày tải lên : 20/02/2014, 11:20

... that could mediate binding to CD11b and probably also to CD11a CD11b I domain inhibits red cell binding to purified integrin Red cells bind poorly to CD11a/CD18 but more efficiently to CD11b/CD18 [28] ... transfectants to both I domains CaCl2 alone was not sufficient to support the maximal binding of any of the ICAM transfectants to the I domain fusion proteins Ó FEBS 2003 Fig Binding of red cells to CD11a/CD18 ... ICAM-4 transfectants to both I domains was efficiently, but not totally abolished, as was also the binding of ICAM-1 transfectants to the CD11b I domain fusion protein The inhibitory effects of EDTA...

14
495
0

Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

Ngày tải lên : 20/02/2014, 11:20

... Palom, Y., He, Q.Y & Tomasz, M (2001) Selective activation of mitomycin A by thiols to form DNA crosslinks and monoadducts: biochemical basis for the modulation of mitomycin cytotoxicity by the quinone ... the GSH, the cytotoxic effect was slightly reduced, by a factor of about three The 50% inhibitory concentration (IC50) value of 2.2 lM measured with S23906-1 alone was increased to 7.1 lM in the ... alkylator, mechlorethamine hydrochloride, we observed a reduction of cytotoxicity by a factor of about six In this case, the IC50 value of 4.2 lM determined with mechlorethamine alone increased to...

12
701
0

Tài liệu Báo cáo khoa học: Steady-state and time-resolved ﬂuorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Ngày tải lên : 20/02/2014, 23:20

... induced by cAMP binding to the anti-cAMP -binding sites of CRP, which in turn trigger specic pathways of signal transmission from the cyclic nucleotide -binding domain to the DNA -binding domain ... binding to the syn-cAMP -binding sites at concentration of the ligand of mM causes a % 6% increase in its uorescence intensity, which indicates that this residue is sensitive to cAMP binding to ... that binding in solution of two cAMP molecules to highafnity anti-cAMP -binding sites at the N-terminal domain causes the C-terminal domain to shift further to the N-terminal domain of CRP To conrm...

11
494
0

$Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt$

Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt

Ngày tải lên : 21/02/2014, 00:20

... blood of normal donors These cells were not sensitive to the cytotoxic effect of GT oligomers Ó FEBS 2003 eEF1A binding to aptameric cytotoxic GT oligomers (Eur J Biochem 270) 3257 Fig SouthWestern ... growth-inhibition effect exerted by cytotoxic GT oligomers and their selective binding to nuclear eEF1A In fact, in normal human lymphocytes no appreciable binding of GT oligomer to nuclear eEF1A was shown ... corresponding to the N-terminal region of eEF1A, were present; this region is totally deleted in PTI-1 Similar considerations were taken into account to exclude the possibility that P1 corresponded to isoforms...

12
552
0

Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

Ngày tải lên : 06/03/2014, 01:20

... corresponded to that obtained in the absence of tHSAheme-Fe(III) (l0 = 2.4 ã 10)1 s)1) Ibuprofen binding to tHSAheme-Fe(II)-NO Figure shows the binding isotherm for ibuprofen binding to tHSAheme-Fe(II)-NO ... at the heme -binding pocket (FA1) Indeed, ibuprofen binding to HSAheme has been reported to induce the hexa-coordination of the heme Fe atom [18,21,23] Thus, peroxynitrite cannot bind to the heme ... according to Eqn (4) [21,32,38,39]: lon ẳ lontopị flontopị ẵibuprofenị=K3 ỵ ẵibuprofenịg 4ị where lon(top) represents the asymptotic value of lon under conditions where [ibuprofen] = (i.e lon(top)...

9
489
0

Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot

Ngày tải lên : 07/03/2014, 05:20

... isotherm and the ﬁtted data for the binding of aPyADP to the enzyme are shown in Fig The binding stoichiometry was close to two Table Binding parameters of coenzymes to 6PGDH from Trypanosoma brucei ... value of the coenzyme [20] The enthalpy change for NADP binding is relatively low, and a positive entropy change contributes to binding For NADPH and aPyADP, the binding appears to be totally enthalpic, ... the coenzyme analogue, a more negative binding enthalpy and, more interestingly, a decrease in the binding stoichiometry of the coenzyme (Table 3) Indeed, only one coenzyme molecule per enzyme...

10
402
0

Báo cáo khoa học: Nucleotide binding to human UMP-CMP kinase using ﬂuorescent derivatives ) a screening based on afﬁnity for the UMP-CMP binding site potx

Ngày tải lên : 07/03/2014, 09:20

... [16]) as uorescent probes to monitor the binding of nucleotides to UCK These assays were used to determine the binding afnity of bisubstrates and new phosphonate analogs and to validate a uorescent ... Competitive uorescence experiments to determine the binding of natural substrates to the donor and the acceptor sites of human UCK The ATP -binding site (donor -binding site) of human UCK was probed ... uorescence of MABA-CDP to its initial value, demonstrating the specicity of MABA-CDP binding to the acceptorbinding site (Fig 3A, inset) Titration of MABA-CDP with the enzyme (Fig 3A) indicated...

11
468
0

Báo cáo khoa học: The enzyme-binding region of human GM2-activator protein pdf

Ngày tải lên : 07/03/2014, 12:20

... mutation C138R distorts the orientation of the helix and compromises the activator’s ability to interact with the enzyme In addition to mapping the activator’s binding interface with the enzyme, our ... is inhibited by GM2AP [5], allowed probing the variant activator’s binding to the enzyme To study the membrane activity and lipid -binding properties, SPR spectroscopy with immobilized lipid bilayers ... membrane-embedded glycolipid substrate Our goal was to map the protein region on GM2AP responsible for binding to the enzyme and to further elucidate the activator’s mode of action at the lipid–water interface...

10
429
0

Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx

Ngày tải lên : 07/03/2014, 12:20

... Upon binding of Ca2+ to the C-lobe, CaM undergoes a ﬁrst conformational change, exposing a hydrophobic binding pocket and, hence, promoting binding to the target (e.g [13,16,17]) Binding to the ... is an additional motif with CaM -binding capacity (BD-C1) CaM binding to this site seems to be weak, but functional assays led us to conclude that this putative binding site is relevant for channel ... showed a weaker binding to TMR-MCGS-(H)6-hCaM in the presence of 25 lm free Table Binding of MCGS-(H)6-hCaM to fusion proteins of cytosolic hEAG1 domains Dissociation constants KD for binding of TMR-MCGS(H)6-hCaM...

13
500
0