enzyme activity and dom pom reactivity
Báo cáo y học: " Hyperglycemia alters enzyme activity and cell number in spinal sensory ganglia" ppsx
... examine the impact of long-standing hyperglycemia and hypoinsulinemia on the distribution and activity of lysosomal acid β-glycerophosphatase (AP), cytochrome oxidase (CO), and NADPH-diaphorase (NADPH-d; ... sensitive to injury and perturbation Therefore, these enzymes were selected as putatively reflective of mitochondrial function (CO), lysosomal or degradative activity (AP) and primary sensory ... pentobarbital, perfused with 4% paraformaldehyde and the lumbar spinal cord and sensory ganglia removed, sectioned and processed for AP, CO or NADPH-d enzyme histochemistry as previously described...
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Báo cáo khoa học: Human airway trypsin-like protease induces amphiregulin release through a mechanism involving protease-activated receptor-2-mediated ERK activation and TNF a-converting enzyme activity in airway epithelial cells doc
... tethered ligand that binds to the receptor and autoactivates it PAR-2 is activated by trypsin and mast cell tryptase and is known to mediate airway inflammation both in vitro [7,8] and in vivo ... (Bubendorf, Switzerland) Cycloheximide was from Wako Pure Chemicals (Osaka, Japan); PD98059 and actinomycin D, Biomol (Plymouth Meeting, PA, USA); U0126, Promega (Madison, WI, USA); and GM6001 and TAPI-1, ... (Qiagen); random hexamers were used to prime the reactions PCR was performed using a TaqManTM Universal PCR Master Mix Kit (Applied Biosystems) with specific primers and probes for AR and b-actin...
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báo cáo hóa học: " Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity" pptx
... and conformation of the enzyme, and result in increasing the enzyme loading on the solid substrate and catalytic efficiency Because the nanomultipods have several pods, and the spaces among the ... of enzyme and for being used in catalytic reaction than the other two, which has great implications for the many ongoing studies of enzyme immobilization and applications of the immobilized enzymes ... 14.38 mM of 4-AAP, and 1.21 mM of hydrogen peroxide, and then reacted at 30°C for The initial catalytic reaction rates of the enzyme in the supernatant and the immobilized enzyme were determined...
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Báo cáo y học: "Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes" pptx
... level and enzyme activity typically has little or no impact on the pathway flux, but often leads to small shifts in the levels of the substrates, products and other ligands of the enzyme, and other ... in transcript and metabolite levels and enzyme activities during the diurnal cycle and an extended night (XN) Distribution of global changes in transcript and metabolite levels and enzyme activities ... expressed as means and standard and and for Levelsto given 148are for and conditions describedhthe numbers replicates data filehWT and retrievedanthe [37].in night = 5) 4, 8, Data and levels data...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
... fragility and oxidative damage of erythrocytes of zinc-deficient rats J Nutr 1997; 127: 1290–1296 Lajos N, Miki N, Sandor S Protein and non-protein sulfhydryls and disulfides in gastric mucosa and ... TCA and resuspended in 1.0 ml of M HCl for blank and M HCl containing 2% 2,4dinitrophenyl hydrazine After incubation for h at 37°C, protein samples were washed with alcohol and ethyl acetate, and ... lipids peroxidation and activities of antioxidants enzymes in patients with dyslipidemia Zegl Lek 2006; 63:738-742 Maria K, Ida F, Marlena B, and Julita C Effects of simvastatin and pravastatin on...
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Tài liệu Physical Activity and Women’s Health pptx
... occupational and leisuretime activity was combined and subjects were grouped into three activity classifications A strong inverse relationship was found between physical activity and CHD mortality ... observational and experimental evidence that physical inactivity plays a significant role in the development of cardiovascular disease in women, and that habitual physical activity and at least ... based programs in schools and communities that are culturally sensitive and ethnic-specific R EF ER EN CE S Albanes, D., Blair, A., and Taylor, P.R (1989) Physical activity and risk of cancer in...
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Tài liệu Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987-2006) docx
... proportional random selection of primary and secondary sampling units (towns and sections, respectively), with the final units (individuals) being selected by means of random routes and sex- and age-based ... sleeping > hours per day and those sleeping < hours per day Statistical analysis In this study we analyzed physical activity and physical fitness separately for men and women and we excluded respondents ... Team: Older adults, chronic disease and leisuretime physical activity Gerontology 2009, 55:64-72 Centers for Disease Control and Prevention: Physical activity and health A report of the Surgeon...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... the wild-type enzyme (100%) and were calculated based on two independent activity measurements on X6 and two independent activity measurements on Xylazyme AX on the same unpurified enzyme sample, ... XBS mutants Activity measurements After the screening procedure, a smaller set of enzymes was selected for purification and further biochemical characterization The activity of these enzymes was ... = 9.76 · 10)10 M and AzU = 50.8 · 10)10 M and for the wild-type XAN, X6U = 1.02 · 10)10 M, XyU = 7.72 · 10)10 M and AzU = 9.65 · 10)10 M for the activity on X6, Xylazyme AX and Azo-wheat AX,...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... purified apo -enzyme and incubated at 70 °C, the enzyme became significantly more active (Table 1) Incubation with the metal at 37 °C did not raise the activity of Table SOD activity Enzyme Activity ... K1 activity in the Mn- and Fe-forms, and the other exhibiting low activity in the Fe-form and high activity in Mn-form ApeSOD belongs to the latter group [16] This was also confirmed by the activity ... the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain (Fig 3) In Mn-reconstituted...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... numbers of the prodomain are designated with the suffix ‘p’) and a catalytic domain (Ala1-Pro364; residues in the mature catalytic domain are numbered without a suffix where unambiguous, and with the ... 38-kDa and 19-kDa proteins were mixed and subjected to nondenaturing PAGE, these proteins comigrated with each other, showing a broad protein band that differed from the respective original bands and ... time-dependent enhancement of the 57-kDa band with a concomitant diminution of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig 3A) The 38-kDa and 19-kDa bands did not disappear completely...
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Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx
... Padgett, W.L., Oshunleti, O., Daly, J.W & Kirk, K.L (2000) Syntheses of (R )and (S)-2- and 6-fluoronorepinephrine and (R)- and (S)-2- and 6-fluoroepinephrine: effect of stereochemistry on fluorine-induced ... Bubendorf, Switzerland) The measurements were performed in 96-well plates with a Fluoroscan plate reader (Labsystems, Helsinki, Finland) Excitation and emission wavelengths were 355 nm and 500 nm, respectively ... and Leu23, which are displaced ˚ 0.5 A with respect to inhibitors 1–5 2- and 3-Fluorobenzyloxy side groups The mono-substituted inhibitors and are fluoro-substituted in the ortho (2-fluoro) and...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... behaviour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and ... with an A leads to substrate behaviour and a low, stable inhibitory activity (Fig and Table 3) This W is located in the cleft between hF and s2A (Fig 5), and the presence of an aromatic side chain ... 100 and 128 are highly conserved among serpins (F119 and F147, respectively, in a1PI) [2] and buried in the hydrophobic interface between hF and b-sheet A (Fig 5) The substrate behaviour and...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... purity ATP-stimulated protease activity in aging rats Fig Lon protease quantification and activity in liver mitochondrial matrix from 10- and 27-month-old rats Activity was determined in the absence ... exhibited a comparable pattern of bands, although two bands of 60 and 150 kDa strongly visible at 10 months (lane 1) were absent at 27 months, and an important band of 70 kDa (lane 2) emerged in ... intense signals of 70 and 50 kDa, while the bands at 60 and 150 kDa vanished With oxyblot (Fig 4C), antibodies stained carbonylated proteins mainly in band ranges of 30–60 and 70–120 kDa at 10...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
... Kdo, and tetrasaccharide 1-monophosphate (TS 1-MP) One-dimensional 1H- and 31P-, and two-dimensional homo- (1H,1H-DQF-COSY) and heteronuclear (1H,13C-,1H,31P-HMQC) NMR spectroscopy (Figs and 6) and ... C18:0, C20:0 and 3-OH-C20:0 MALDI-MS and ESI-MS analysis As a first step in the structural analysis, LPS and de-Oacylated LPS of C trachomatis serotype E was analyzed by MALDI-TOF and ESI-FT-ICR ... from C trachomatis serotype E and L2 were of similar activity and both less active than smooth LPS by a factor of 100 whereas, LPS from Chl psittaci was of lower activity than the two other chlamydial...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... on their effects on stability and activity parameters of RNase-A Functional stability and activity by osmolytes Results and Discussion Protein stability and enzyme activity have a well-correlated ... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its consequent ... reactions of the enzyme substrate, enzyme intermediate and enzyme product complexes [29] On the other hand, kcat ⁄ Km is an apparent second-order rate constant that refers to the properties and the reaction...
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Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
... polyacrylamide gel in denaturing and reducing conditions, and by western blotting (A) Coomassie-stained bands of isolated Hpt (lane 1), standard ApoE (lane 2), standard ApoA-I (lane 3), and partially purified ... HRP-conjugated avidin and ECL Coomassiestained bands of VLDL and LDL proteins are shown in lanes and 2, respectively VLDL and LDL proteins, blotted onto the PVDF membrane and incubated with biotinylated ... anti-Hpt IgG and rabbit anti-goat HRP-conjugated IgG Two bands, reacting with the antibodies and with the molecular masses of b and a2 (41 and 21 kDa, respectively), were detected (Fig 4, lanes and 6)...
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
... mutations from both the stability and activity mutants, and demonstrated a significant improvement in both stability and catalytic activity (Fig 1) As such, the activity and stability mutation combinations ... remaining activity, mutants AS1–AS7 were selected on the basis of a co-improvement in stability and activity For example, the T1 ⁄ values for AS4 and AS6 were 6.7 and 11.2 °C higher, respectively, and ... wild-type protein and two mutants, AS4 and AS7, were purified to homogeneity with purification yields of over 40%, and investigated for their stability and activity at temperatures between 40 and 80 °C...
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Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
... MCL, Miranda MCS & Barbosa MA (2005) Adsorption of a therapeutic enzyme to selfassembled monolayers: effect of surface chemistry and solution pH on the amount and activity of adsorbed enzyme Biomaterials ... protein engineering by rational design and ⁄ or random mutagenesis of hyperthermostable enzymes may result in even more thermostable enzymes Several enzymes have already replaced many traditional ... hyperthermophilic enzyme that normally functions at higher temperatures Utilizing this approach would obviously avoid the expensive and laborious enzyme engineering process, and revolutionize industrial and...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... ATPase domain and C-terminal helix [36] showing the location of residues that were mutated The TRP, RDF and DEL sequences are shown in green, dark blue and red, respectively The b domain and C-terminal ... C-terminal helix, as well as the b-domain DEL sequence, for Vps4p ATPase activity, wild-type and mutant Vps4p proteins were purified (Fig 4A) and the ATPase activity of each Vps4p protein was ... of Vps4p–E233Q and ⁄ or Vta1p did not stimulate the ATPase activity of the b domain mutant proteins (Vps4p–GAI and Vps4p–DEL), which cannot bind Vta1p We conclude that Vps4p–RDF and Vps4p–E233Q...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... screening Error-prone PCR and DNA shuffling were separately used to create a randomized library of protein variants based on the Q143A hMnSOD template One hundred and fifty thousand QC774 transformants ... screening of libraries generated by random mutagenesis (errorprone PCR and DNA shuffling) of the Q143A hMnSOD gene Amino acid substitutions (capital letters) and base pair substitutions (small letters) ... activity and efficiency Although even higher catalytic activity would have been desirable, this result demonstrates that our directed evolution approach can indeed be used to engineer mutant enzymes...
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