enzymatic browning
Advances in understanding of enzymatic browning in harvested
... in-volvement of POD in enzymatic browning of litchi fruit.The success of commercial sulphite treatment incontrolling litchi pericarp browning is an evidence forthe hypothesis that the browning is due ... anthocyanidindegradation, resulting in enzymatic browning (Fig. 1).With an increasing research effort on litchi, our under-standing of the enzymatic browning mechanism in thefruit pericarp ... this unique and popular fruit.Post-harvest browning of litchi fruit skin is the mainlimitation to market acceptance. The biochemistry of enzymatic browning has not yet been fully elaborated(Jiang...
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Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers
... viewthelackofaberrantphenotypesassociatedwithreducedPPOexpressionsuggeststhattheapproachdescribedheremaybebroadlyapplicabletothereductionof enzymatic browning inarangeofcommerciallyimportantplantsandtheirprocessedproducts.ExperimentalProtocolPlantmaterials.Potatoplants(Solariumtuberosumcv.VanGochandDiamant)weregrowninvitroonMSmedium:6supplementedwith30g/1sucrose.PotatointernodcexplantsweretransformedwithAgrobacteriumtumefactens(strainGV3101)-7containingtheantisense-PPOTi-plasmidconstructsusingtheco-cultivationmethodessentiallyaccordingtoprotocolsdescribed1".Plantmaterialformolecularanalysiswastakenfromplantsgrownin17cmpotsundergreenhouseconditions.Poly-A*RNAforNorthernanalysiswasisolatedfromthefirsttwointernodcleavesfromstolonsinitiatingtuberizationwith3-5mmswollentipsandfromtubersof1-2cmdiameterharvestedattheonsetoffloweringMolecularbiology.RoutineDNAmanipulationswereasdescribedby™Aiat'™A•Southern'NorthernandWesternanalysesofpotatoDNA,PPOtranscriptsandproteins,respectively,wascarriedoutasdescribedpreviously".SubstratesforsequencingwereproducedusingtheinvivoexcisionprotocolonlambdaZAPIIclones(Stratagene,LaJola,CA)isolatedfromasinktubercDNAlibrarykindlysuppliedbyLWillmitzer.Poly-A*RNAwasextractedusingpoly-d[T],«oligonucleotidescoupledtoparamagneticbeads(DynalA.S.Oslo,Norwav)Fivehundredngpoly-A*RNAwasloadedperlaneandclectophoreticallyseparatedRNAwascapillaryblottedontoHybondN*membrane(Amersham,UK)andprobedwithaninternalDNAfragmentofthePPOcDNApKG59-4labeledwith32P.Proteinwasextractedfromabout6gofmicrotubertissueofthetransgeniclinesused.10pgofproteinwasloadedperlane.TandemCoomassieblue-stainedgelswereruntoverifyeaualloading. ... significant reductions.•/RESEARCHAntisenseExpressionofPolyphenolOxidaseGenesInhibits Enzymatic Browning inPotatoTubersChristianW.B.Bachem",Gert-JanSpeckmann1,PietC.G.vanderLinde2-5,FrankT.M.verheggen2,MichelleD.Hunt3,JohnC.Steffens3andMarcZabeau1*•KeygeneN.V.,AgroBusinessPark90,P.O.Box216,6700AEWageningen,TheNetherlands.:RZResearchB.V.,P.O.Box2,9123ZRMetslawier,TheNetherlands.3CornellUniversity,252EmersonHall,IthacaNY14853-2703,USA.Presentaddresses:"DepartmentofPlantBreeding,UniversityofWageningen,P.O.Box386,6700AJWageningen,TheNetherlands.5StichtingBedrijfslaboratoriumvoorWeefseikweek,P.O.Box52,2371ABRoelofarendsveen,TheNetherlands.*Correspondingauthor.Spoilagecausedby ... reductions.•/RESEARCHAntisenseExpressionofPolyphenolOxidaseGenesInhibits Enzymatic Browning inPotatoTubersChristianW.B.Bachem",Gert-JanSpeckmann1,PietC.G.vanderLinde2-5,FrankT.M.verheggen2,MichelleD.Hunt3,JohnC.Steffens3andMarcZabeau1*•KeygeneN.V.,AgroBusinessPark90,P.O.Box216,6700AEWageningen,TheNetherlands.:RZResearchB.V.,P.O.Box2,9123ZRMetslawier,TheNetherlands.3CornellUniversity,252EmersonHall,IthacaNY14853-2703,USA.Presentaddresses:"DepartmentofPlantBreeding,UniversityofWageningen,P.O.Box386,6700AJWageningen,TheNetherlands.5StichtingBedrijfslaboratoriumvoorWeefseikweek,P.O.Box52,2371ABRoelofarendsveen,TheNetherlands.*Correspondingauthor.Spoilagecausedby post-harvest enzymatic browning is a problem ofconsiderable importancetofoodgrowers, processors and retailers. Here...
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Control of enzymatic browning in apple with ascorbic aci
... 1002-JOURNAL OF FOOD SCIENCE-Volume 54, No. 4, 1989 CONTROL OF ENZYMATIC BROWNING IN APPLE. . . Table 5-Inhibition of enzymatic browning in Granny Smith juice by cyclodaxtrins (CD) and combinations ... concentrations greater than 0.5 mM, prevented browning for over 7 hr. With plugs, 10 mM CINN inhibited browning for several hours but then induced severe browning over extended storage times (Table ... short-term protection against browning in Red Deli- cious and Winesap plugs but induced browning in samples stored 6 or more hours (data not shown). As with CINN, in- duced browning by BENZ may be...
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Enzymatic browning in apples
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Enzymatic browning in foods
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enzymatic browning
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... withthe OGOR and LDH reactions. Fdox, oxidized ferredoxin; Fdred,reduced ferredoxin.Table 1. Enzymatic reactions.Enzyme Reaction catalyzed by the enzymeaPOR (reductivecarboxylation)Acetyl-CoA ... Lindqvist Y (1998) Crystallography andmutagenesis of transketolase: mechanistic implicationsfor enzymatic thiamin catalysis. Biochim Biophys Acta1385, 387–398.49 Furdui C & Ragsdale SW (2002) ... Electrochemical oxidation of enaminesrelated to the key intermediate on thiamin diphosphatedependent enzymatic pathways: evidence for one-elec-tron oxidation via a thiazolium cation radical. JAmChem...
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Tài liệu Browning and His Century doc
... influence of an Aprile, yet like Browning& apos;s ancient Greek,Cleon, he longed for a solution of the mystery.At the dawn of the century, and in his youth, Browning ventured upon a solution. ... maypropound.If we see the thoughtful and brilliant Browning in the "Fancies" proper, we perhaps see even more clearly theemotional and passionate Browning in the lyrics which add variety and ... twolovers whom we are probably justified in regarding as Mr. and Mrs. Browning. One naturally thinks of themas companion pictures to Mrs. Browning& apos;s "Sonnets from the Portuguese." In these...
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Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc
... controlling these dual enzymatic functions; initial data indicate that low-molecular-weight compound(s) direct silicatein to either the cata-bolic or the anabolic reaction. Enzymatic parametersof ... organic matrix. Siliceoussponges (Demospongiae) have the exceptional abilityto synthesize silica enzymatically via silicatein [7,8].Based on its protein sequence, silicatein is related tothe ... time that recombinant silicatein acts as a silica polymerase andalso as a silica esterase. The enzymatic polymerization ⁄ polycondensation ofsilicic acid follows a distinct course. In addition,...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
... plasmin in the presenceof long-chain fatty acidsIdentification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulationAnna Tanka-Salamon1, Kiril Tenekedjiev2, Raymund...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
... effect.The catalytic properties of the different FNRmutants were determined for two different enzymatic reactions. The observed values for kcatand Kmandthe calculated kcat⁄ Kmvalue ... significantlypopulated. Tm, DSmand DDG values were determined asdescribed previously [67]. Enzymatic assaysFNR-dependent diaphorase and cytochrome c reductaseactivities were determined ... in Table 4, theoxidase activity of the mutants was not affected to thesame extent as the other enzymatic activities. Matteviet al. [34] have suggested that the rate-limiting step inthe oxygen...
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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc
... Bioinformatic and enzymatic characterizationof the MAPEG superfamilyAnders Bresell1,*, Rolf Weinander2,*, Gerd ... overexpressing recom-binant SynMGST were solubilized in Triton X-100.The recombinant SynMGST was also enzymaticallyactive upon detergent solubilization and the CDNBconjugating activity was used to ... coli and V. cholerae sequences form aseparate group [1]. Nothing is known, however, aboutthe enzymatic properties of any prokaryotic MAPEGprotein.As the number of sequenced bacterial genomes...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... Semi-Dry Cell (BioRad, Hercules, CA, Enzymatic properties of chitinase A from Vibrio carchariae W. Suginta et al.3384 FEBS Journal 272 (2005) 3376–3386 ª 2005 FEBS Enzymatic properties of wild-type ... mgÆmL)10.07 ± 0.006 –aDetermined by colorimetric assay,bdetermined by HPLC-ESI MS.W. Suginta et al. Enzymatic properties of chitinase A from Vibrio carchariaeFEBS Journal 272 (2005) 3376–3386 ª 2005 ... with one proton) map to the chromatographic peaks of the respective molecules.W. Suginta et al. Enzymatic properties of chitinase A from Vibrio carchariaeFEBS Journal 272 (2005) 3376–3386 ª 2005...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25