enhanced mismatch mutation analysis simultaneous detection of point mutations and large scale rearrangements by cap
Báo cáo y học: " Multi-faceted, multi-versatile microarray: simultaneous detection of many viruses and their expression profiles" pps
Ngày tải lên :
13/08/2014, 13:20
... for the diagnosis of viral infections First, this chip provides a more accurate diagnosis of viral infection by simultaneously evaluating the transcription of all viral genes, and can use such ... instance, cell lines latently infected with HIV-1 and KSHV were used to generate profiles of viral gene expression in the presence of cyclin-dependent Page of (page number not for citation purposes) ... but also by the structure of chromatin The latter can be vary depending on the post-translational modifications of histone proteins Methylation, acetylation, and/ or phosphorylation of certain...
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Báo cáo hóa học: " Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer" pdf
Ngày tải lên :
18/06/2014, 16:20
... E746-A750del and L858R point mutation from New Zealand rabbits and evaluated them by Western blotting, immunofluorescence and IHC They tested these antibodies in a series of cell lines and in tumor ... laboratories, and depend on the quality of the samples IHC is a standardized assay of simple methodology and high sensitivity and specificity, and the development of specific antibodies against EGFR mutation ... characteristics of patients and the findings of seven studies examining EGFR mutations by IHC, including the present study Although the most common EGFR mutations are the 15-bp ELREA deletion in exon 19 and...
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Báo cáo khoa học: "Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction" ppsx
Ngày tải lên :
07/08/2014, 18:21
... dna siralullecartni L fo noitceted rof RCP xelpitlum fo yticificepS giF AND fo gp 05 ;5 enaL ,AND fo gp 001 ;4 enaL ,AND fo gn ;3 enaL ,AND fo gn 01 ;2 enaL ,AND etalpmet fo gn 001 ;1 enaL ,III ... 67031CCTA AND siralullecartni.L *SQRVN secruoS sniarts lairetcaB secruos dna )AND( sniarts lairetcab fo tsiL elba T RCP fo ecneserp ehT nim rof C 27 ta noitaziremylop lanoitidda yb dewollof ,nim rof ... stnemgarf AND deifilpma cificepsnon yna ecudorp ton did dna ,sniarts lairetcab hcae deifirup AND etalpmet htiw tnemgarf AND detcepxe eht decudorp yassa RCP ehT snoitcaer RCP hcae ni AND etalpmet...
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báo cáo khoa học: " Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay" potx
Ngày tải lên :
11/08/2014, 00:22
... Wavelength (nm) 700 750 Figure Design of the dual-label time-resolved immunofluorometric assay (A) A schematic illustration of the assay for simultaneous detection of HIV and HBV infections The Arabic ... anti-HIV-1 antibody- and HBsAg-binders in a duallabel assay will not compromise the sensitivity of detection of either analyte This is further borne out by the analysis of sera that contain HBsAg ... supported by grants from the Department of Biotechnology, Government of India and Academy of Finland (grant #115524) under a joint Indo-Finnish collaborative research programme SMT was the recipient of...
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Analysis and detection of human emotion and stress from speech signals
Ngày tải lên :
15/09/2015, 22:03
... ANALYSIS AND DETECTION OF HUMAN EMOTION AND STRESS FROM SPEECH SIGNALS TIN LAY NWE (B.E (Electronics), Yangon Institute of Technology) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m th filter bank St ( m ) ... corpuses of emotional speech and stressed speech are described This is followed by an experimental review and analysis of traditional acoustic features and pattern classifiers in Chapter Feature analysis, ...
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Xpert MTB/RIF test for detection of pulmonary tuberculosis and rifampicin resistance (Protocol) pptx
Ngày tải lên :
06/03/2014, 04:20
... percentage of contaminated cultures; review question B: the name and manufacturer of the reference standard • Details of index test: software version of test • Details of comparator: type of microscopy: ... each of the QUADAS items Statistical analysis and data synthesis The first step in data analysis will be a descriptive analysis of the results of the primary studies For both review question A and ... 2010a) Early and accurate diagnosis and effective treatment is the cornerstone of TB care and control (Dye 2010) A basic tenet of early and accurate diagnosis is the identification of smear-negative...
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
Ngày tải lên :
07/03/2014, 00:20
... investigate the simultaneous improvement of the activity and stability of Symbiobacterium toebii TPL, as a better structural understanding of how proteins respond to mutations and recombination ... of A1–A4 and S1–S3 allowed the parental origin of the mutations to be estimated For example, AS4, composed of A13V, E83K and T451A, was analyzed to be a reassembly of S3 (harboring the A13V mutation) , ... the mutation of Thr15 to Ala in the vicinity of the hydrophobic core induces a tighter binding of the cofactor in C freundii TPL, thereby reducing the decomposition rate of the cofactor by a...
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Báo cáo khoa học: Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe pdf
Ngày tải lên :
16/03/2014, 11:20
... for the analysis of O2–Æ, the effect of buffer solution and the concentration of the fluorescent probe were investigated Effect of pH and buffer concentration The pH of the medium has a large effect ... buffer for the incubation of mammalian cells, and may improve the planting efficiency of cell incubation and breed ability of cell We showed that the optimal pH for O2–Æ detection was in the range ... Effects of other ROS and biological compounds Fig A linear correlation between the fluorescence intensity and O2–Æ concentration To assess the selectivity of the method, the effect of other ROS and...
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Báo cáo " Moving parabolic approximation model of point clouds and its application " pdf
Ngày tải lên :
28/03/2014, 10:20
... scanning data set of a mouse which contains 36036 points, and presented the point- based reconstruction and the estimates of curvature in Figure The results show the confidence of our MPA method ... The first fundamental form of S*(u,v) is given by * 2 I = Edu + 2Fdudv + Gdv , (6) where E =1, F =0 and G =1 at the foot -point ox The second form of S*(u,v) is given by II * = Ldu + 2Mdudv + Ndv ... of this work is a scheme to generate a point- based reconstruction of an unorganized point cloud and simultaneously to estimate the differential properties of the underlying surface As an application,...
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Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt
Ngày tải lên :
30/03/2014, 20:20
... homogeneity of isochore can be defined by an index h [17,20], which is defined as the variance of GC content of the region divided by that of the whole genome If h ( 1, the variance of GC content of the ... orange dots in Fig 1, and they are located in 0–230 kb of chromosomes II and 0–350 kb of chromosomes IV, respectively The similar genomic organization of chromosomes I, III and V makes their overall ... types of isochores were distinct in the distribution of gene density, T-DNA insertion site and transposable element [17] In this study, we also use the cumulative GC profile proposed by Zhang and...
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Báo cáo khoa học: " Improved Automatic Detection of Zero Subjects and Impersonal Constructions in Spanish" docx
Ngày tải lên :
31/03/2014, 21:20
... precision of 74% and recall u of 60%, and K-nearest neighbors in TiMBL: both in (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of ... definitions and values tense, and mood with the preceding verb in the sentence and also with the main verb of the sentence.3 7-9 NHPREV, NHTOT, INF: the candidates for the subject of the clause ... both by Connexor’s Machinese Syntax parser and a set of lists We can describe each of the features as broadly belonging to one of ten classes, as follows: PARSER: the presence or absence of a...
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Báo cáo hóa học: " Incidence of the V600K mutation among melanoma patients with BRAF mutations, and potential therapeutic response to the specific BRAF inhibitor PLX4032" potx
Ngày tải lên :
18/06/2014, 16:20
... between the V600E and V600K mutations Our preclinical data demonstrating similar kinase activity of the V600K and V600E mutations, together with clear evidence of clinical activity of PLX4032 in ... Application of a BRAF pyrosequencing assay for mutation detection and copy number analysis in malignant melanoma J Mol Diagn 2007, 9:464-471 Hay R, MacRae E, Barber D, Khalil M, Demetrick D: BRAF mutations ... Holden J, Tripp S, Layfield L: Human malignant melanoma: detection of BRAF- and c-kit-activating mutations by highresolution amplicon melting analysis Hum Pathol 2005, 36:486-493 10 Ugurel S, Thirumaran...
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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc
Ngày tải lên :
18/06/2014, 19:20
... evaluate and compare the modulations of PBMC global gene expression profiles induced by IFNa in melanoma and normal donors The effects of the administration of different doses of IFNa, as well as of ... [32] Page of 15 liquid nitrogen tank Samples of a single donor for each time -point (T0, T0+24, T1 and T1+24) were thawed and processed simultaneously Number of viable cells was evaluated by trypan-blue ... and anti-CD86 (Pharmingen) Samples were collected and analyzed by using a FACSCalibur (Becton Dickinson) and data analysis was performed by FlowJo software (Tree-Star), excluding dead cells and...
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Báo cáo hóa học: " Simultaneous circulation of genotypes I and III of dengue virus 3 in Colombia" docx
Ngày tải lên :
20/06/2014, 01:20
... Philippines China Philippines Japan Malaysia Thailand Thailand Thailand Thailand Thailand Thailand Thailand Malaysia Thailand Thailand Thailand Thailand Bangladesh Bangladesh India SriLanka Martinique ... Philippi56 JaponImp73 Malaysia92 Thailand81 Thailand85 Thailand80 Thailan87a Thailan87b Thailand89 Thailand91 Malaysia94 Thailand97 Thailand96 Thailand92 Thailand94 Banglade01 Banglade00 India84 ... Venezuelan origin is supported by high similarity of sequences and circulation of this genotype in Venezuela in August of 2001, when the largest epidemic caused by DENV there since the 1989 DENV-2...
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Label free and reagentless electrochemical detection of microRNAs using a conducting polymer nanostructured by carbon nanotubes application to prostate cancer biomarker mir 141
Ngày tải lên :
02/07/2014, 14:14
... electrochemical detection of miR141, a miRNA biomarker It is shown that the copolymer electroactivity is enhanced by the presence of o-MWCNTs, which probably participate to the low detection limit and high ... on SWNTs and an immobilized redox probe This system allows very specific detection of DNA but the limit of detection is only 0.1 mM 3D structures obtained using CNTs may induce high capacitance ... CNTs preparation MWCNTs were purified and oxidized in a 1:1 mixture of HNO3 and H2SO4 at 90 1C for h, then washed with distilled water until pH and separated by centrifugation The solid residue...
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Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript" ppt
Ngày tải lên :
09/08/2014, 20:20
... levels, facilitated the discovery of novel splice variants, and enabled detection of novel fusion transcripts and isoforms thereof that would otherwise have escaped detection As such, this method ... coverage of low-abundance transcripts It also circumvents certain limitations of targeted genomic DNA sequencing, in which detection of chromosomal rearrangements may be challenging (and analysis of ... Conclusions By combining hybridization capture of cDNAs and next-generation sequencing, targeted RNA-Seq provides an efficient and cost-effective means to analyze a specific subset of a transcriptome simultaneously...
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báo cáo khoa học: "C60-Fullerenes: detection of intracellular photoluminescence and lack of cytotoxic effects" pdf
Ngày tải lên :
11/08/2014, 00:22
... Szwarc H: Quantitative analysis of C-60 fullerene in blood and tissues by high-performance liquid chromatography with photodiode-array and mass spectrometric detection Journal of Chromatography B-Analytical ... are taken up by cells and intracellular C60 retains its optical properties, as determined by measurements of PL Significantly, our studies reveal that C60 prepared by a variety of methods up ... of fullerene clusters not reaggregate, and have a range of sizes of tens of nanometers, although some larger clusters also exist TEM data differ from that of the dynamic light scattering results...
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báo cáo khoa học: " High-throughput analysis of chromosome translocations and other genome rearrangements in epithelial cancers" pdf
Ngày tải lên :
11/08/2014, 12:20
... problem of distinguishing driver and passenger mutations is as acute for structural mutations as it is for point mutations [10-13] Stephens et al [5] estimate that approximately 2% of genome rearrangements ... mutations, that is, random mutational noise, but some must be selected, ‘driver’ events and, as the number and variety of known mutations increases, estimates for the number of ‘driving’ mutations in ... rearrangements of the types they found would generate an in-frame fusion gene by chance They observed 1.6%, which suggests that the majority of gene fusions, like the majority of point mutations, ...
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Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx
Ngày tải lên :
12/08/2014, 04:20
... Page of Figure The specificity and sensitivity of one step SYBR green real-time RT-PCR Plot of the amplification of a 10-fold serial dilution of ch-1a RNA to calculate the detection limit and ... serially to 10-7 and were assayed in a 25 μl reaction mixture containing μl of diluted RNA; 0.4 μM of Nf primer and 0.4 μM of Nr primer; 12.5 μl of 2× One Step SYBR RT-PCR Buffer; μl of PrimeScript™ ... RT-PCR for detection of porcine reproductive and respiratory syndrome virus Virology Journal 2010, 7:90 Figure The sensitivity of conventional RT-PCR for detection of the PRRSV The detection limit...
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