empcr enrichment and dna bead loading
Báo cáo y học: "Comparison of dot chromosome sequences from D. melanogaster and D. virilis reveals an enrichment of DNA transposon sequences in heterochromatic domains" docx
... (DINE-1) DNA 126 201 631 34.5 yakuba_cons (DINE-1) DNA 54 170 722 12.4 FB4_DM DNA 630 1,215 6.8 PROTOP DNA 938 3,615 6.4 PROTOP_A (1360) DNA 518 1,111 6.3 DMCR1A LINE 528 1,253 5.7 PROTOP_B DNA 14 ... Dorer and Henikoff [63] showed that DNA transposon mobilization events can lead to tandem and inverse duplications of the P element These tandem arrays can lead to heterochromatin formation and ... the contig made up of DNA transposons (separated into 1360, DINE-1 and all other DNA transposons), retroelements, simple repeats, and repeats of unknown type (see Materials and methods) Total repeats...
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The Ins and Outs of Loading External Assets
... layers, tweens, movie clip instances, and scripting every time you need to make a change Your project becomes more dynamic, offering each user a unique experience By loading external assets, you can ... getting video (and MP3) content into your project, as we'll discuss later in this lesson In the following exercises, you'll learn how to load a variety of external file types, and how to control ... employing a wider range of content that loads based on time of day, month, user input, or even a randomly generated number Although it would be next to impossible to create a single SWF that could...
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Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc
... DNA- cleaving and RNA-cleaving DNAzyme (DRc DNAzyme) We elected to use an in vitro-selected pistol-like (PL) DNAzyme as a DNA scaffold for designing a DNAzyme with dual catalytic activities The PL DNAzyme ... FEBS D Jiang et al An RNA-cleaving and DNA- cleaving DNAzyme A B C D Fig The DNA- cleaving activity and cleavage sites of DRc DNAzyme (A) 5¢-32P-labeled DRc DNAzyme was incubated in buffer A at ... 8–17 variant DNAzyme The stem and terminal loop (5¢-AGC-3¢) of 8–17 variant DNAzyme replaces stem–loop I of the PL DNAzyme scaffold, and the T deoxyribonucleotide of 8–17 variant DNAzyme is inserted...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... An equimolar amount of complementary strand was added, and the mixture was heated for at 96 C and slowly cooled to room temperature The double-stranded DNA was stored at )20 C in experimental ... 1ị where SD and SRF are the respective areas under the emission spectra of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor ... 10PỵDAị=2 2ị where F and Fcor are uorescence intensity before and after the correction, and P and DA denote the initial sample absorbance at the excitation wavelength and the change in absorbance...
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..GENE CLONING AND DNA ANALYSIS..GENE CLONING AND DNA ANALYSISAn IntroductionT.A. BROWNFaculty of Life Sciences University of Manchester ManchesterSixth EditionA John Wiley & Sons, Ltd., Publication. pdf
... Plasmids and Bacteriophages Pilus M13 particle injects DNA into cell (a) Injection of singlestranded DNA into the host cell, followed by synthesis of the second strand Single-stranded DNA Double-stranded ... pure DNA DNA Contaminants Pure DNA (b) Separation of DNA from contaminants + Pure DNA Contaminants Part I The Basic Principles of Gene Cloning and DNA Analysis 30 Figure 3.6 Aqueous layer (DNA ... Basic Principles of Gene Cloning and DNA Analysis 18 Phage particle DNA The phage attaches to the bacterium and injects its DNA Phage DNA molecule The phage DNA molecule is replicated Capsid...
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Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein doc
... salt Lanes and show naked DNA digestion patterns, and lanes 3–8 represent chromatin R3 is absent in lanes 1, 3, and 6, whereas chromatin was assembled at 50 mM salt for lanes 1–4, and at 70 mM ... assembled at 90 and 110 mM salt concentrations Naked DNA (lanes and 5) and chromatin assembled in the absence or presence of R3 protein are shown R3 was added to lanes 2, 3, and (C) IEL analysis ... top strand between L1 and L2 and deformation of DNA at the core ends may result in the less clear protection (Fig 5B,C, lane 8, lower panel) in the IEL analysis, which revealed the doublestrand...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
... properties of C1q toward DNA and of an emerging technique allowing the molecular combing of DNA strands and its observation by single-molecule spectroscopy Double- and single-stranded DNA has been demonstrated ... fluorescent beads (C1r*), and finally DNA was combed We observed that C1r* spots were aligned along the DNA strands (Fig 2E) Thus C1r binds to the DNA C1q assembly, whereas C1r does not bind to DNA strands ... (Hamamatsu) and IXON software (Andor), with an exposition time ranging from 100 ms to s Results Interaction between DNA and C1q DNA is described as an activator of human complement system [30] The DNA- dependent...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... of 0.005 and growth was followed for at least ®ve generations before sampling All samples were withdrawn between D540 0.2 and 0.4 Enzymes Restriction enzymes, T4 DNA ligase, and T4 DNA polymerase ... were obtained from and used as recommended by New England Biolabs and Boehringer Mannheim Plasmid and ATP, ADP extraction Aliquots (0.8 mL) were removed from cell cultures and placed into an ... 5¢TCCATAGCAGCGGCGAAACCA-3¢ and chromosomal DNA from strain LM1237 [17] as a template The PCR fragment was subsequently digested with the enzymes DraI and EcoRV and a 842-bp fragment containing the DNA region...
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GENE CLONING AND DNA ANALYSIS pptx
... Plasmids and Bacteriophages Pilus M13 particle injects DNA into cell (a) Injection of singlestranded DNA into the host cell, followed by synthesis of the second strand Single-stranded DNA Double-stranded ... pure DNA DNA Contaminants Pure DNA (b) Separation of DNA from contaminants + Pure DNA Contaminants Part I The Basic Principles of Gene Cloning and DNA Analysis 30 Figure 3.6 Aqueous layer (DNA ... Basic Principles of Gene Cloning and DNA Analysis 18 Phage particle DNA The phage attaches to the bacterium and injects its DNA Phage DNA molecule The phage DNA molecule is replicated Capsid...
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Báo cáo khoa học: Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix–loop–helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms docx
... 412 and 538 nm at pH 8.0 The absorption maxima of the Fe(II) species were at 423, 530 and 558 nm, and those of the Fe(II)–CO complex were at 420, 530 and 568 nm It is likely that Fe(II) and Fe(II)–CO ... at 421 nm and 543 nm at pH 8.0 The absorption maxima of the Fe(II) species were at 426, 530 and 559 nm, and those of the Fe(II)–CO complex were at 422, 538 and 566 nm On the other hand, optical ... Fe(III) and Fe(II) complexes were assigned as the redox-sensitive m4 band [17,32] For the Fe(III) complex, the spin-coordination and coordination-state marker bands (m3) were located at 1470, 1492 and...
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scientific american - 2004 06 - nanotech and dna
... extent in the borderlands of northern England–southern Scotland and from Ulster, in contrast with the Puritan culture of New England, which originated in southern England (Rice and Feldman could ... would make four strands of DNA For each strand, the sequence along half of the strand would match half of a second strand and the remaining half would match half of a third strand [see lower illustration ... your left hand on the inner banister and your right hand on the outer one Such a staircase is a right-handed helix Conventional right-handed DNA is called B -DNA and is the most energetically favored...
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Báo cáo hóa học: " Role of protease-activated receptor-2 on cell death and DNA fragmentation in Helicobacter pylori-infected gastric epithelial cells" ppt
... death and DNA fragmentation in gastric epithelial cells To investigate the relations of PAR-2 expression, cell death, and DNA fragmentation, cells were transfected with AS ODN for PAR-2 and cultured ... for 24 hours DNA fragmentation was determined by the amount of oligonucleosomebound DNA in the cell lysates The relative increase in oligonucleosome-bound DNA was determined at 405 nm and expressed ... of apoptotic cell death, the expression of PAR-2, and the activation of MAPK in H pylori-infected gastric epithelial cells, cell viability and DNA fragmentation were determined in the cells transfected...
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Báo cáo vật lý: "Effect of Treated and Untreated Filler Loading on the Mechanical, Morphological, and Water Absorption Properties of Water Hyacinth FibersLow Density Polyethylene Composites" pdf
... effect of fiber loading on the water sorption constants n and k Table 2: The dependence of water sorption constants n and k on fiber loading for LDPE/WHF composites Type Filler loading (wt%) n ... Fibers 0 15 25 Filler loading (phr) Figure 1: Effect of filler loading on the tensile strength of the LDPE/WHF composites with untreated and treated WHF However, as the filler loading continued to ... the LDPE/WHF composites with untreated and treated WHF Effect of Treated and Untreated Filler Loading 92 Figure shows Young’s modulus for the pure LDPE and LDPE/WHF composites Young’s modulus...
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Báo cáo y học: "Nucleosome deposition and DNA methylation at coding region boundarie" doc
... oftheand highergene expression and for and region in yeastfileofin thestart codon and levelcodons codinghigh DNA inside genes withsurroundingto differential codon, Pol II Illustrative data UTR and ... GSTMBD2b protein, His-MBD3L1 protein, and the JM110 bacterial DNA MagneGST beads (Promega, Madison, WI, USA) were pre-blocked with the JM110 bacterial DNA and incubated at 4°C in the MIRA binding ... transcription initiation and termination sites, and coding start and end sites were obtained from the RefSeq Genes track of the UCSC Genome Browser [25] for the human, mouse, and fly genomes Each...
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Báo cáo y học: "Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus" pot
... Donadi E: Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control ... phosphorylation (ATP6, COX1, COX3, CYTB, ND1, and ND2), in which three are relevant to mitochondrial dysfunction Because the expressions of DNA repair and mtDNAencoded genes were found downregulated, ... COX3, ND1, ND2, and ATP6), the expression levels of X-ray repair cross-complementing 6, COX2, and ATP8 were also decreased in patients with SLE (Figures and 4) Several ATPases and ATP synthases...
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Báo cáo y học: "Comparative determination of HIV-1 co-receptor tropism by Enhanced Sensitivity Trofile, gp120 V3-loop RNA and DNA genotyping" ppt
... (0.110-0.166) (n = 2,946 pairs) and DNA- DNA (n = 2,874) distance was 0.121 (0.095-0.152) The median (IQR) intrapatient RNA-RNA (n = 56), DNA- DNA (n = 52) and paired RNA -DNA (n = 120) distances were ... Study population and samples We processed 178 samples (99 plasma RNA and 79 whole-blood DNA) and successfully amplified 155 samples [78 (78.8%) plasma RNA and 77 (97.5%) whole-blood DNA] from 55 ... 40%, 88.2%, and 96.2% at < = 50 cp/ml, 51-500 cp/ml, and >500 cp/ml respectively The rate of successful sequencing from DNA was 95.1%, 100%, and 100% at < = 50 cp/ml, 50-500 cp/ml, and >500 cp/ml,...
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Báo cáo y học: "A comparative approach for the investigation of biological information processing: An examination of the structure and function of computer hard drives and DNA" doc
... The reference system for identifying and Figure Organized cluster mapping of DNA to Nucleus Mapping of DNA strand into DNA Hard Drive: A) shows the DNA strand decomposed into its information structure ... comparative analysis between the architecture and function of the CHD and the structure and function of eukaryotic DNA, which we now define as the DNA hard drive (DHD) The computer ATA (Advanced ... D’Onofrio and An Theoretical Biology and Medical Modelling 2010, 7:3 http://www.tbiomed.com/content/7/1/3 Conclusions: The comparison of functional and structural characteristics of the DNA complex and...
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Báo cáo sinh học: "The use of communal rearing of families and DNA pooling in aquaculture genomic selection schemes pptx
... the Ncand selection candidates; and SIB_TRAIT, a trait that is measured on Ntest test individuals (sibs of the candidates), which were sacrificed to record the SIB_TRAIT The Ncand selection candidates ... of (Ncand/Nfam) selection candidates and a second group of (Ntest/Nfam) test individuals (Ncand = 20000 and Ntest = 2000, 20000 or 50000) Hence, family sizes were (Ncand+Ntest)/Nfam offspring ... sorted into the H-pool and the 50% lowest Page of into the L-pool DNA of the H-pool was extracted, pooled and genotyped Similarly the L-pool’s DNA was extracted, pooled and genotyped, which resulted...
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Báo cáo sinh học: "Combined therapy with cyclophosphamide and DNA preparation inhibits the tumor growth in mice" docx
... treatment of CP and human genomic double stranded DNA (dsDNA) fragmented to 200–6000 bp [26] This combined treatment was much more effective than treatment with CP alone When exogenous DNA was used ... The DNA preparation did not contain histones and polysaccharides; it was endotoxinfree Mouse DNA was isolated from a mixture of tissues (thymus, liver, kidneys, spleen) and salmon sperm DNA was ... treatment with cytostatic and DNA (Fig 3) Mice received human DNA preparations every hour for 12 h after CP injections (Regimen 4) and hourly for h, 13–18, 19–24, 25–30, and 31–36 h after CP injections...
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Báo cáo y học: "Genomic analysis of the relationship between gene expression variation and DNA polymorphism in Drosophila simulans" pps
... between π and expression variation is strong for the coding regions and 3'UTRs, weak for introns and 5'UTRs, and is absent for CPRs These results are robust to different bin sizes (Materials and methods) ... fixed effect and replicate (RNA prep) as a random effect The total number of genes analyzed after the removal of polymorphic and diverged probes and absent probesets was 7,949 for males and 7,128 ... collected and analyzed data, and wrote the paper AH analyzed data and wrote the paper DB helped design experiments and edited the paper CJ helped design experiments, analyzed data and edited...
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