elisa for quantitative determination of igg in purified anti dt antibody
Hollow fiber protected microextraction for the determination of pollutants in complex matrices
Ngày tải lên :
22/10/2015, 21:08
... simple to use and effective for the determination of OCPs in milk 28 CHAPTER Determination of Pollutants in Soil 4.1 Determination of polycyclic aromatic hydrocarbons in soil by hollow fiber-protected ... first introduced in segmented-flow determination[ 1] It is based on the injection of a liquid sample into a moving, nonsegmented continuous carrier stream of a suitable liquid Then the injected ... funnel for extraction and at the same time as a syringe for direct injection of the extract into a GC or GC-MS for analysis The main feature of this method was the use of smaller amounts of the...
- 79
- 290
- 0
Review of the Existing Techniques for the Determination of Dry Rubber Content in Natural potx
Ngày tải lên :
23/03/2014, 21:21
... is in the range of to 10 percent However, this method is still in use in rubber estates for assessing the ORC of latex for making sheets on a large scale and also for calculating the quantity of ... discussion of the results are included in chapter five of this thesis Finally, in chapter six the summary and general conclusions of the work including scope for doing further work in the area are incorporated ... Travancore during 1860s and subsequently in Cochin for the distribution of forest and wasteland for plantation crops were instrumental for the initial growth and expansion'l", In terms of productivity,...
- 32
- 890
- 2
Báo cáo hóa học: " Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies" docx
Ngày tải lên :
20/06/2014, 02:20
... there is only one type of infectious and replicating HCV The variety of HCV RNA in patients could result from changes induced in the plasma/sera, including the production of noninfectious viruses ... is, therefore, well suited for studying the biology and immunology of HCV infections It is also an excellent system for testing the efficacy of candidate drugs and advancing ideas for vaccines Methods ... titer Finally, the isolated HCV should be capable of infecting new target cells without transfection A molecular analysis of California Institute of Molecular Medicine isolated HCV (CIMM-HCV) for...
- 15
- 340
- 0
Báo cáo khoa học: "Comparative efficacy of standard AGID, CCIE and competitive ELISA for detecting bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan, India" potx
Ngày tải lên :
07/08/2014, 18:21
... resistant to BTV infection, in the Rajasthan State of India Since, the serological testing of small ruminants for anti- BTV antibodies is still mainly based on AGID test, we also investigated the ... 78 Smriti Shringi, B N Shringi Table Details of local breeds of sheep screened for presence of antibodies against BTV using standard AGID test Breed accounting for 20 per cent of the total global ... obtained in this study, it appears to us that cELISA is highly specific and sensitive test and should be used for routine monitoring status of BTV infection in local breeds of small ruminants in...
- 3
- 298
- 0
Báo cáo khoa học: "Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine" pptx
Ngày tải lên :
07/08/2014, 18:21
... saw noitcaer cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( STBA etartsbus eht fo emulov lacitnedi na ,h rof Co73 ta noitabucni ... 1.0/05DICT 002 gniniatnoc noisnepsus suriv 9981VK a fo emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ... sisongaid -ores eht ni esu laitnetop sti rof detagitsevni saw ASILE-I VEJ eht rof depoleved saw dohtem lacigolores tneinevnoc dna elpmis a ,nosaer siht roF selpmas fo rebmun detimil a htiw noitazidradnats...
- 5
- 429
- 0
báo cáo khoa học: " Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant " ppsx
Ngày tải lên :
11/08/2014, 11:21
... Fluo-3 into inflorescences as a useful tool for in situ Ca2+ localization in the intact pistils Results Experimental design In situ detection of Ca2+ in olive pistils was carried out by direct injection ... measure intracellular Ca2+ ions by using microscopy imaging techniques To introduce this dye into intact pistils, we injected the Fluo-3 solution directly into olive inflorescences To Page of 12 ... solution of mM Fluo-3 AM in DMSO The final DMSO concentration in the incubation solution was approximately 1% (v/v) Injection was done directly into the peduncle of the inflorescence at the site of...
- 12
- 529
- 0
Báo cáo khoa học: "Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera" docx
Ngày tải lên :
12/08/2014, 18:22
... Proficiency testing among european laboratories using an immunofluorescent antibody test (IFAT) for the detection of antibodies against Lawsonia intracellularis in pigs Proceedings of the 16th International ... but since shedding in growing pigs may occur at short intervals [21,26], and circulating antibodies may be detected for weeks only [3,21], the consistently high levels of antibody found in older ... Schoeder H, Ohlinger VF: A new blocking ELISA kit for the detection of antibodies specific to Lawsonia intracellularis in porcine blood samples the 4th International Veterinary Vaccines and Diagnostics...
- 6
- 288
- 0
Báo cáo khoa học: "SOFA is superior to MOD score for the determination of non-neurologic organ dysfunction in patients with severe traumatic brain injury: a cohort study" doc
Ngày tải lên :
13/08/2014, 02:24
... hypotension MAP 5a or epinephrine ≤0.1a or norepinephrine ≤0.1a Dopamine >15a or epinephrine >0.1a or norepinephrine >0.1a Hematologic: platelet ... intervention in the form of blood pressure augmentation to maintain cerebral blood flow Page of 10 (page number not for citation purposes) Surprisingly, neither SOFA-defined nor MOD-defined respiratory ... further categorized as having SOFA-defined and MOD-defined cardiovascular failure, SOFA-defined but not MOD-defined cardiovascular failure, MOD-defined but not SOFA-defined cardiovascular failure,...
- 10
- 388
- 0
Light sheet based fluorescence correlation and cross correlation spectroscopy for quantitative measurements of bio molecules in live cells
Ngày tải lên :
09/09/2015, 11:19
... players in biological systems to enable understanding in protein-protein interactions or protein-DNA interactions A number of techniques, including protein microarrays, pull down assays and chromatin ... CROSS-CORRELATION SPECTROSCOPY FOR QUANTITATIVE MEASUREMENTS OF BIO-MOLECULES IN LIVE CELLS ANAND PRATAP SINGH (M.Sc Chemistry, BHU, INDIA) NATIONAL UNIVERSITY OF SINGAPORE 2014 is Th pa ge in io nt te lly ... imaging method can provide spatio-temporal dynamics and binding maps of proteins localized in membrane, cytosol and nucleus This particular study combines fast SPIM imaging (single plane illumination...
- 135
- 606
- 0
Liquid phase microextraction for the determination of acidic drugs and beta blockers in water samples
Ngày tải lên :
10/11/2015, 11:00
... and Ms Wu Jingming for their help during the course of this project Special thanks to Xiaofeng for her insight to the project; Junie for proofreading this thesis; Elaine and Debbie for their friendship ... important role in maintaining the integrity of the extraction system by ensuring proper organic solvent immobilization and preventing direct mixing of donor phase with acceptor phase in three-phase ... of the analytes In this study, an acidic pH maintained the NSAIDs and clofibric acid in their extractable molecular forms Various concentrations of HCl were used instead of varying the pH value...
- 74
- 277
- 0
Guidelines for the determination of brain death in infants and children
Ngày tải lên :
14/11/2016, 06:25
... Guidelines for the Determination of Brain Death in Infants and Children Critical Care Medicine 2011;39:2139–2155 Nakagawa TA, Ashwal SA, Mathur M, Mysore M Guidelines for the Determination of Brain Death ... for determination of brain death in children American Academy of Pediatrics Task Force on Brain Death in Children Pediatrics 1987;80:298–300 Nakagawa TA, Ashwal SA, Mathur M, Mysore M Guidelines ... has resulted in irreversible coma Coma and apnea must coexist to diagnose brain death Determination of brain death by neurologic examination should be performed in the setting of normal age-appropriate...
- 15
- 293
- 0
Báo cáo y học: "Thioglycosides as inhibitors of hSGLT1 and hSGLT2: Potential therapeutic agents for the control of hyperglycemia in diabetes"
Ngày tải lên :
26/10/2012, 10:04
... hSGLT1, suggesting that differences in the aglycon binding site play an important role in the inhibitory effect of this substance In contrast, differences in the sugar binding site resulted in an preferen- ... metabolized in the intestine [27] Therefore, a chemical reaction to convert the substance in its active form, after intestinal absorption like in the case of O-glucosides, is not necessary In addition, ... determine the amount of ATP in mg of protein measured from the number of cells per well After 24 h, the microtiter plate was taken for scintillation counting of radioactive [14C]AMG using a MicroBeta...
- 9
- 650
- 0
Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
Ngày tải lên :
05/09/2013, 10:15
... Edetic acid (EDTA) in Drinking-water, Background document for development of WHO Guideline for Drinking-water Quality, 2nd ed Addendum to vol Health criteria and other supporting information, World ... Examination of Water Occurrence of EDTA in river water samples from three regions of Japan was examined EDTA was detected in ten of thirteen river water samples A high level of EDTA was observed in river ... Methods for the Examintion of Water would be to perform an inter-laboratory validation study of the proposed method Determination of EDTA in river water samples For the application of this analytical...
- 7
- 689
- 0
Tài liệu OUTLINES OF DAIRY BACTERIOLOGY A CONCISE MANUAL FOR THE USE OF STUDENTS IN DAIRYING docx
Ngày tải lên :
16/02/2014, 22:20
... composed of an almost infinite number of individual germs, the result of the continued growth of a single organism that was in the drop of milk which was firmly held in place when the gelatin solidified ... Fig 14 Effect of cooling milk on the growth of bacteria Conn[42] is inclined to regard temperature of more significance in determining the keeping quality than the original infection of the milk ... action of various kinds of life Many of these are of great service in destroying or holding bacterial growth in check Those that are toxic and result in the death of the cell are known as disinfectants;...
- 201
- 540
- 0
Tài liệu Personal Exposure to Air Pollution for Various Modes of Transport in Auckland, New Zealand doc
Ngày tải lên :
18/02/2014, 04:20
... higher than those of cyclists, noting that they are also highly variable depending on an individual’s level of fitness and individual choice of intensity of physical exercise In addition to the ... 36 L min-1 (within the range of 24-60 L min-1) and the runner a moderate level of 48 L min-1, both within the range of values reported in the literature [21-23] The commuter ‘dose’ was defined ... 2012, Volume instead of controlling the amount of traffic on the existing roads Congestion charging schemes such as those introduced in Singapore and London, which have lead to a decrease of 40% and...
- 9
- 394
- 0
Tài liệu Guideline on Best Practice for the Audit of Risk in Public/Private Partnership (PPP) docx
Ngày tải lên :
18/02/2014, 05:20
... XVI INCOSAI in 1998) • guidelines on best practice for the audit of economic regulation (adopted by XVII INCOSAI in 2001); and • guidelines on best practice for the audit of public/private finance ... regulation INCOSAI XVII asked the Working Group to examine whether further guidance on the new forms of partnership – including issues arising when the state is a minority shareholder in private businesses ... are in place • Obtain an independent, expert valuation of the assets • Ensure that issues of profit sharing (including possible future gains from property sales or refinancing) are addressed...
- 30
- 441
- 0
Tài liệu Báo cáo khoa học: "Phrase-Based Backoff Models for Machine Translation of Highly Inflected Languages" docx
Ngày tải lên :
22/02/2014, 02:20
... backoff models for combining in- domain and 42 out -of- domain data for the purpose of bootstrapping a part -of- speech tagger for Chinese, outperforming standard methods such as EM i i i Backoff ... consist of 2000 sentences each In order to study the effect of varying amounts of training data we created several training partitions consisting of random selections of a subset of the full training ... differences to the baseline model are shown in boldface (p < 0.05) Set train1 train2 train3 train4 train5 Set train1 train2 train3 train4 train5 German-English baseline backoff BLEU PER BLEU PER...
- 8
- 379
- 0
Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc
Ngày tải lên :
07/03/2014, 05:20
... samples used in the determination of GSH were obtained In order to evaluate the applicability of the proposed method, uorescence determination in plasma was performed according to the following procedures ... Ueland PM (1992) Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma Anal Biochem 200, 218229 11 Szejtli J (1998) Introduction and ... Glutathione determination using bis(b-CD)s Table Interferences of various coexisting biological substances Coexisting substance 10 20 30 40 Time (min) 50 60 Fig Effect of reaction time on uorescence intensity...
- 8
- 429
- 0
Báo cáo khoa học: New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases pot
Ngày tải lên :
15/03/2014, 23:20
... distribution of BsArb43B in the proximity of the active site Arabinotriose in the binding cleft is represented using sticks (B) Detailed view of the binding cleft with arabinotriose (C) Details of the ... hairpin that joins blades IV and V in the N-terminal domain and interacts with the C-terminal domain is shown in red catalytic domain with the Ca trace of BsArb43A from B subtilis (Protein Data ... arabinohexose [15], this loop makes the reducing end of the carbohydrate chain bend towards the solvent, probably optimizing binding of the carbohydrate chain in the cleft, and resulting in trioses...
- 13
- 568
- 0
Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf
Ngày tải lên :
16/03/2014, 18:20
... pea seed proteins (CP, control proteins); molecular mass of protein standards is indicated in kDa (Std) (B) Immunoblotting of the same proteins with polyclonal antibody against ferritin (PAAF) (C) ... bovine serum albumin, 20% (v/v) normal goat serum in 0.1 M Trisbuffered saline (pH 7.4), and then incubated for h in Trisbuffered saline (pH 7.4) containing PAAF (diluted : 5), 1% (w/v) bovine ... lL of rabbit polyclonal antibody raised against pea seed ferritin (PAAF, described in [22]) was added After incubation for h at °C, 50 lL of protein-A sepharose (50% v/v slurry, washed twice in...
- 8
- 504
- 0