... was added to it, the tube was shaken, and, the methyl ester of PHA monomers in the chloroform layer was collected The chloroform phase was washed again with 0.5mL water, and then injected to ... reactors, the value of conversion rate to PHA, CRP value, was calculated as the amount of PHA increased duringthe anaerobic phase divided by the amount of carbon supplied as influent in one cycle For ... storage is one ofthe important processes forthe DOC removal in activated sludge CONCLUSIONS The occurrence of temporal carbon storage material, PHA, was confirmed in the course of wastewater...
... apoptotic markers duringthe late stages of MPyV infection However, comparison ofthe levels of apoptotic markers and cytotoxicity duringthe replication of wt MPyV, and replication ofthe virus mutated ... as a control of sample loadings Discussion In the present work, the cytotoxic properties ofthe minor structural proteins (VP2 and VP3) ofthe MPyV were studied in the absence of other virus components ... inducers of apoptosis The low toxicity of tVP3, observed duringthe first 24 h post-transfection, suggests that domain ofthe minor capsid proteins may be important forthe potentiation of apoptosis...
... their names down, their mothers urging them to it; you would have thought they were setting out forthe Olympian games It was the same with the young men on the other side ofthe Rhine, and there ... morality upon you; its State acts for you, its fashions and its opinions steal from youthe very air you breathe; you have no lungs, no heart, no light of your own You serve what you despise, you lie ... official ties, they, at least should have the advantages of their independence; but unfortunately few of them are able to judge for themselves of events which are beyond the limits of their habitual...
... for RNA 2614 GAT TGT AA GG CCC ATC TA GAT GGA GGG RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR Control Control forforforforforforforfor expression of pri-miR9-1 expression of ... involved in the transcriptional regulation of neuronal miRs [24–26] Unfortunately, the upstream region ofthe pri-miR9-1 does not have any homologue-binding sequence forthe transcription factors ... indicate the position and orientation ofthe primers Bold font represents the sequence of mature miR9* and italic fonts the sequence of mature miR9 (C) RT-PCR analysis of pri-miR9s duringthe neuronal...
... the clones yield the full-length cDNA sequences of at least two distinct LDH-A isoforms, which differ substantially in the coding sequence and length ofthe 3¢-UTR Therefore, isoform-specific PCR ... ofthe LDH gene has been found Sequence divergence between pairs of isoforms, known in diploid teleosts is $ 20–25% (for ORFs of mlc1 and mlc2 genes), while divergence between the two forms of ... in their 3¢-UTR sequence binding sites for specific proteins that increase ⁄ decrease the rate of poly(A) shortening, i.e affect the lifetime ofthe mRNA [32], therefore it is likely that the...
... the absence ofthe SK fragment (B inset) Dependence with the pH ofthe wavelength ofthe maximum ofthe fluorescence spectra forthe ANS + SK 1–63 mixtures relative to the spectrum of free ANS ... in the vertical axis for clarity The length ofthe vertical segment in each panel represents 30 kJÆK)1Æmol)1 on the vertical axis of scan rate forthe rest ofthe DSC curves A decrease in the ... discrepancies in their account ofthe number of unfolding units involved [4,13–20] One ofthe reasons for this disagreement might arise from the fact that in some of these studies the number of independent...
... line, the incomprehensible title of "Young Lion ofthe Fold." Young Lion ofthe Fold, Says the Shan Van Vocht, Young Lion ofthe Fold, Says the Shan Van Vocht; Young Lion ofthe Fold, Bade us the ... wine ofthe country," and then he mourns over the desolation ofthe land and the ravages ofthe so-called "crowbar brigade" as if they were things of yesterday Whether the local Press reflects the ... and their agents are determined to make another effort to get in their rents in January Their view ofthe case is that the law must assist them: but whatever abstract idea ofthe majesty of the...
... requires There can be no complaint as to the strength ofthe coffee, since there is no milk to dilute it, but the color ofthe sugar might afford ground of exception on the part ofthe over-fastidious ... fifty thousand ofthe freemen of Pennsylvania, under the terms ofthe proclamation ofthe previous day, to repel the rebel invasion Immediately after the publication ofthe proclamation of Wednesday, ... necessity for prompt action on the part ofthe State authorities On the 10th of September, the Governor, acting under the direction ofthe President ofthe United States, issued another proclamation,...
... function of (OC/EC)E for winter and summer Again, the critical point ofthe curves gives the representative value of (OC/EC)E for that time of year [64] The median value of (OC/EC)E determined forthe ... compounds Forthe summer data, the location of this minimum coincides with the maximum r2 value forthe photochemically produced tracer species We interpret the location ofthe critical value of r2 ... Series II GC The temperature program forthe GC oven was: 35°C for min, 3°C/min to 95°C, 12.5°C/min to 195°C, hold forThe oven then ramped down to 35°C in preparation forthe next run The carrier...
... effect on A) There is no significant effect ofthe age ofthe firm It is clear that the size ofthe firm in total assets is the key determinant ofthe amount of advertising Large firms have the financial ... al (1995) for a further discussion ofthe relation between order of entry and performance barriers were about absent as there was a lack of supervision ofthe Central Bank The number of commercial ... used forthe conclusion The rise and fall ofthe number of saving banks Many commercial banks entered in Russia following the 1988 banking reform This was to a large extent the consequence of the...
... It was the popular Federal parody of "Gay and Happy:" "Then let the South fling aloft what it will, We are forthe Union still! Forthe Union! Forthe Union! We are forthe Union still!" The bride ... the same grave." I spoke ofthe difficulty of recognition, ofthe remoteness ofthe field, and ofthe expense attending the recovery of any remains, particularly those ofthe enemy, that, left hastily ... eyes and active figures ofthe dance, the sad uncertainties ofthe time Youth trips lightest, somehow, on the brink ofthe grave The hilarities ofthe evening so influenced the German quartered...
... related to the age of weaning, i.e the shorter the duration of breastfeeding, the earlier the onset of deficiency (73) This is likely due to the combined effect ofthe consumption of complementary ... trivial source of error); and failures to account for either the non-protein component of human milk or the possible under-utilization of some ofthe milk’s protein constituents because of their resistance ... months The reason given for introducing complementary feeding before the age of to months was the infant’s demand appeared greater than the supply of human milk This was decided by the mother in...
... son ofthe ex-President The legal struggle was one ofthe most celebrated in the annals ofthe New York bar There was abundant evidence of moral delinquency on the part of both parties to the ... Chancellor ofthe New York University, President of Rutgers College, a candidate forthe Vice Presidency ofthe United States, and President ofthe American Bible Society A grandson ofthe signer was the ... when they come to act) be decisive in the opinion ofthe present members as to the complexion ofthe next house There are other points of view which I cannot now state to you, in which the result...
... the bottom Antibodies used and temperature for culture are indicated at the top In the bottom, the ratio (39 ⁄ 33 °C) ofthe intensity ofthe signals is shown (B) A dominant-negative mutant of ... decreased at the nonpermissive temperature To address the question of whether CDK2 is responsible forthe phosphorylation of MCM4 at CDK sites during interphase, a dominant-negative mutant of CDK2 ... phosphorylation of MCM4, together with the presence of geminin, which inhibits the ability of CDT1 to load MCM proteins onto chromatin, may help prevent re-replication of DNA in the G2 and M phase During...
... attacks the phosphorylated form of hCdc6p However, more importantly in the present context, we note that the synthesis of hCdc6p continues through most ofthe cell cycle Synthesis of hCdc6p during ... and that about one half ofthe newly synthesized hCdc6p associated with chromatin duringthe 2-h label period Rates of hCdc6p synthesis To investigate whether the synthesis of hCdc6p was restricted ... [35S]methionine for h at the beginning (0–2 h after thymidine-block) and in the middle of S phase (4–6 h), as well as at the end of mitosis and duringthe early G1 phase (12–14 h) ofthe next cycle...
... The development ofthe DGE system enables the sequencing of total cDNA forthe derivation of an accurate measure of gene expression, both individually and comprehensively, and the discovery of ... which accounted for 24% of genes, whereas only 11.3% of genes were classified into this category in the brace root tissue Forthe rest ofthe categories, the transcript abundances in the brace root ... developed forthe functional description of proteins The presence of identified functional domains by blastx search in the examined sequence was accepted forthe assignment of functions A criterion of...
... RISK AND THE COST OF BANK FUNDING Since the beginning ofthe sovereign debt crisis the effectiveness ofthe bank lending channel forthe transmission ofthe monetary policy stimulus to the economy ... The multidimensional nature ofthe current crisis has therefore complicated the analysis of financing conditions The assessment of financing conditions in the euro area against the background of ... observed forthe non-investment grade segments ofthe corporate bond market – albeit for these segments the cost of market debt was significantly higher Moreover, for these segments, the rise...
... accounts ofthe effect upon the mother country ofthe destruction ofthe tea The ministry had carried through Parliament their system of repressive measures: the Boston Port Bill, the revision ofthe ... counteract the tendency of their conclusions They are constantly visible in the letters of this volume, even in the midst ofthe severest trials They form what may be considered the romantic turn ofthe ... than ofthe adventurous and hardy sons of labor, who carve out for themselves a new home in the forests ofthe West The return of Mr Adams, with his family, to the United States, the liberty for...
... these marks were attenuated forthe rest ofthe cell cycle (10 and 20 h), correlating with the expression ofthe gene (Fig 6C,D) The correlation of promoter occupancy of MLL1, H3K4 trimethylation ... 6C,D) In the case ofthe HoxA7 gene promoter, the recruitment of RNAP II and MLL1 and the level of H3K4 trimethylation were low at the beginning (S phase) and gradually increased as the cell ... Dynamics of MLL1 and its interacting proteins duringthe cell cycle Prior to the analysis ofthe dynamics of MLL and histone methylation, we synchronized HeLa cells at different phases ofthe cell...