... Accumulation of mitochondrial P450MT2, NH(2)-terminal truncated cytochrome P4501 A1 in rat brain during chronic treatment with beta-naphthoflavone A role in the metabolism of neuroactive drugs J Biol Chem ... on cytochromeP450metabolism and selectivity Drug Metab Dispos 28, 1524–1532 22 Monier S, Van LP, Kreibich G, Sabatini DD & Adesnik M (1988) Signals for the incorporation and orientation of cytochrome ... polymorphisms of cytochromeP450 2D6 (CYP2D6): clinical consequences, evolutionary aspects and functional diversity Pharmacogenomics J 5, 6–13 Zanger UM, Raimundo S & Eichelbaum M (2004) Cytochrome P450...
... (mol·mol–1 P450scc) Secosteroid (mol·mol–1 P450scc) A Metabolism of vitamin D3 by cytochrome P450scc 25 Vitamin D3 20 15 10 20(OH)D3 20,23(OH)2D3 0 50 100 150 200 Time (min) B Secosteroid (mol·mol–1 P450scc) ... 2008 FEBS 2587 Metabolism of vitamin D3 by cytochrome P450scc R C Tuckey et al Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by P450scc A Secosteroid (mol·mol–1 P450scc) 40 20(OH)D3 ... D3 with P450scc may, however, correspond to this product (Fig 8A) Metabolism of vitamin D3 by cytochrome P450scc Comparing the major pathways of vitamin D3 and cholesterol metabolism by P450scc,...
... functional cytochromeP450 produced and is thus an index of its stability Enzyme activities Each incubation initially contained house fly cytochrome b5 (1 nmol) [42], house fly cytochrome P450- reductase ... functional cytochromeP450 (ratio values of 0.44 and 0.37, respectively) The membrane preparations from bacteria transformed with pCW did not reveal any cytochromeP450 after a spectral analysis Metabolism ... in relation to protein sequence in an insect cytochromeP450 is CYP6B1v1 This cytochromeP450 from Papilio polyxenes is involved in furanocoumarin metabolism It has been demonstrated that three...
... substrate, and the local level of P450scc activity Experimental procedures Side-chain modification of vitamin D3 by reconstituted cytochrome P450scc Bovine cytochrome P450scc and adrenododoxin reductase ... 4080–4090 ª 2005 FEBS P450scc hydroxylates vitamin D3 Fig Metabolism of vitamin D3 by purified bovine P450scc Incubations were carried out in a reconstituted system comprising purified P450scc (3 lM), ... activities of P450scc therefore give rise to a new family of products whose biological activity remains to be determined We now further characterize this pathway using bovine cytochrome P450scc with...
... metabolized through N-demethylation by mammal cytochromeP450 [32] In order to gain insight into the P450metabolism mechanism of N-Me-cyclo-peptides, the metabolism of a set of molecules composed ... standard The P450 concentration was measured as described by Omura and Sato [40] MetabolismMetabolism of TTX derivatives was studied at 37 C in 0.1 m phosphate buffer (pH 7.4) with 1.0 lm P450 from ... structure and mechanism of cytochromeP450 enzymes Chem Rev 105, 22792328 Meunier B, de Visser SP & Shaik S (2004) Mechanism of oxidation reactions catalyzed by cytochromep450 enzymes Chem Rev 104,...
... Streptomyces cytochromeP450 – cytochromeP450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2) J Biol Chem 282, 17486– 17500 The role of P450 in microbial fatty acid metabolism ... extracts revealed a clear upregulation of an alkane-inducible cytochromeP450 (AJ273607) The role of P450 in microbial fatty acid metabolism during the first hours of incubation [22] According ... of cytochromeP450 monooxygenases is indicated by grey arrows FEBS Journal 278 (2011) 206–221 ª 2010 The Authors Journal compilation ª 2010 FEBS 209 The role of P450 in microbial fatty acid metabolism...
... of drugmetabolism and disposition These concepts are intended to build a unique foundation of knowledge for drugmetabolism and the subsequent studies performed during drug discovery and drug ... DRUGMETABOLISM AND DISPOSITION Quantitative In Vitro ADME Assays Using LC–MS as a Part of Early DrugMetabolism Screening 383 385 Walter Korfmacher 13 High-Resolution Mass Spectrometry and Drug ... magnetic resonance analysis Now, drugmetabolism plays a critical role in the drug discovery and development process from lead optimization to clinical drugdrug interaction studies Commercialized...
... Kalra BS: CytochromeP450 enzyme isoforms and their therapeutic implications: an update Indian J Med Sci 2007, 61:102-116 Guengerich FP: Cytochrome P450s and other enzymes in drugmetabolism ... T, Price A: The Effect of CytochromeP450Metabolism on Drug Response, Interactions, and Adverse Effects Am Fam Physicians 2007, 76:391-396 Guengerich FP: Cytochromep450 and chemical toxicology ... inhibition of cytochromeP450 3A4 and the role of therapeutic drug monitoring Ther Drug Monit 2007, 29:687-710 Masubuchi Y, Horie T: Toxicological significance of mechanismbased inactivation of cytochrome...
... the p38 inhibitor SB203580 were used The conversion of DHEA into 7α-OH-DHEA is catalyzed by cytochromep450 enzyme 7b (Cyp7b) [8] Because of the reported immunostimulating effects of 7α-OH-DHEA, ... formation of [3H]-7α-hydroxy-dehydroepiandrosterone (7α-OH-DHEA) from [3H]-DHEA, representing cytochromep450 enzyme 7b (Cyp7b) activity, was determined by high-performance liquid chromatography ... dimethylsulfoxide (DMSO), respectively, and used as controls *P < 0.05 (Student's t-test) Cyp7b = cytochromep450 enzyme 7b Cyp7b activity to basal 7α-OH-DHEA levels (Fig 4b) In contrast, addition of...
... regulation of hepatic cytochromeP450 in chronic renal failure J Am Soc Nephrol 2001, 12:326-332 Dreisbach AW, Lertora JJ: Effect of chronic renal failure on hepatic drugmetabolism and drug disposition ... effects of CKD on drugmetabolism [11-13,15] When interpreting the findings presented in Table 2, one must recognize that although AKI may not demonstrate a change in hepatic drug metabolism, it ... Animal studies investigating the effect of AKI on hepatic drugmetabolismDrug Animal AKI model Authors’ conclusion on effect of AKI on hepatic metabolism Ajmaline [18] Rat Uranyl nitrate ↔ Clarithromycin...
... significance Curr Drug Metab 2, 199–214 23 Bhagwat SV, Boyd MR & Ravindranath V (1995) RatBrain Cytochrome- P450 – reassessment of monooxygenase activities and cytochrome- P450 Levels DrugMetabolism ... MA (1995) Microsomal cytochrome P450- mediated liver and brain anandamide metabolism Biochem Pharmacol 50, 677–686 22 Strobel HW, Thompson CM & Antonovic L (2001) Cytochromes P450 in brain: Function ... detection of cytochromes P450, and in particular, members of the CYP4 family It was therefore of great interest when two human ESTs were detected, corresponding to a novel cytochromeP450 Cloning...
... in P450 catalysis Contributions to specicity in drugmetabolismDrug Metab Dispos 26, 11901193 Vaz, A.D.N (2001) Multiple oxidants in cytochrome P450catalyzed reactions: implications for drugmetabolism ... amine functions by cytochromeP450 as means of bioactivation and detoxication Drug Metab Rev 34, 451 477 128 Lewis, D.F.V (1998) The P450 catalytic cycle and oxygenation mechanism Drug Metab Rev ... mechanisms in cytochromeP450 catalysis Biochem Biophys Res Commun 312, 163168 Ortiz de Montellano, P.R & Stearns, R.A (1987) Timing of the radical recombination step in cytochromeP450 catalysis...
... Nelson DR (1995) Diversity and evolution of plant P450 and P450- reductases Drug Metabol Drug Interact 12, 189–206 36 Nelson DR (2006) Plant cytochrome P450s from moss to poplar Phytochem Rev 5, 193–204 ... in plants of a cytochromeP450 able to epoxidize the double bonds of fatty acids The terminal olefin 11-dodecenoic acid is converted into 11,12-epoxylauric acid by a cytochromeP450 in Vicia sativa ... plant P450 reductase in order to optimize electron transfer during catalysis and probably to increase the stability of the expressed P450 Furthermore, there are only three cytochromes P450 encoded...
... interaction of NADPHcytochrome P450 oxidoreductase with cytochromeP450 and cytochrome c J Biol Chem 270, 2747527480 27 Shen AL & Kasper CB (1996) Role of Ser457 of NADPHcytochrome P450 oxidoreductase ... of NADPHcytochrome P450 oxidoreductase into distinct functional domains Proc Natl Acad Sci USA 91, 8710 8714 Hubbard PA, Shen AL, Paschke R, Kasper CB & Kim JJ (2001) NADPHcytochrome P450 oxidoreductase ... of human NADPHcytochrome P450 reductase Biochemistry 40, 19561963 20 Sevrioukova IF & Peterson JA (1995) Reaction of carbon monoxide and molecular oxygen with P450terp (CYP108) and P450BM-3 (CYP102)...
... 271) References Nelson, D.R (1999) CytochromeP450 and the individuality of species Arch Biochem Biophys 369, 1–10 Omura, T (1999) Forty years of cytochromeP450 Biochem Biophys Res Commun 266, ... heme-distal pocket of cytochrome P450nor is essential for interaction with NADH J Biol Chem 276, 5020–5026 Zhang, L., Kudo, T., Takaya, N & Shoun, H (2002) The B’ helix determines cytochrome P450nor specificity ... of cytochrome P-450nor Appl Environ Microbiol 61, 883–889 Zhang, L., Takaya, N., Kitazume, T., Kondo, T & Shoun, H (2001) Purification and cDNA cloning of nitric oxide reductase cytochrome P450nor...
... distinct from other purified bacterial P450 systems which either utilize a Fe2S2 ferredoxin-like P450cam [23] or belong to the microsomal type of P450s like P450BM3 [24] and are reduced by a diflavin ... Da for NHis-P450mor The UV-Vis spectrum of NHis-P450mor was identical to that of wild-type P450mor, isolated previously from Mycobacterium sp strain HE5 [15] In contrast to wild-type P450mor, which ... change in the spectrum of P450mor This may be due to the polarity and hydrophilicity of these compounds in contrast to all other known substrates of P450 cytochromes For P450cam it has been shown...
... catalysed by human cytochromeP450 17a-hydroxylase J Clin Endocrin Metab 77, 98–102 Jefcoate, C.R (1986) Cytochrome P-450 enzymes in sterol biosynthesis and metabolism In Cytochrome P450: Structure, ... Concentrations of cytochromeP450 and cytochrome b5 in baboon adrenal microsomes The dithionite reduced carbon monoxide vs oxidized difference spectrum of baboon adrenal microsomal cytochromeP450 is given ... deficiency as well as from homology alignments with bacterial cytochromes P450 [10] The degree of amino acid sequence homology between cytochromes P450 of humans and other species ranges from 48 to 71%,...
... recently [5] (Eqn 1) # Â Ã exp kon;i Á t Á ð½CO0 À P450 0 Þ À DAi ðtÞ ¼ e Á ‘ Á P450 0 Â Ã P450 0 À ½P450CO1 exp kon;i Á t Á ð½CO0 À P450 0 Þ À ½CO0 " described [14] The absorbance ratio ... substrate-free P450cam stock solution was 1.1 mM To have comparable conditions to previous ð1Þ [P450] 0, [P450CO]1, [CO]0, e, and l are the initial P450 concentration, the final P450- CO concentration, ... conditions are summarized in Table In contrast to P450cam bound with 1R-camphor, norcamphor and norbornane, the time curves for substrate-free P450cam and P450cam bound with camphane could not be fitted...
... kinetic mechanisms of cytochromeP450 enzymes Biochemistry 33, 2927–2937 12 Ortiz de Montellano PR & De Voss JJ (2005) Substrate oxidation by cytochromeP450 enzymes In Cytochrome P450: Structure, ... (2005) Cytochrome P450: Structure, Mechanism, and Biochemistry, 3rd edn Kluwer Academic ⁄ Plenum Publishers, New York, USA Rendic S (2002) Summary of information on human CYP enzymes: Human P450metabolism ... Rate-limiting steps in cytochromeP450 catalysis Biol Chem 383, 1553–1564 18 Guengerich FP, Krauser JA & Johnson WW (2004) Rate-limiting steps in oxidations catalyzed by rabbit cytochromeP450 1A2 Biochemistry...