drug interaction mediated by transporters
Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
... apoptosis by using 3T3-L1 adipocytes and rat primary adipocytes Adipocyte apoptosis could be induced by troglitazone, pioglitazone or 15-deoxy-D12,14-prostaglandin J2 (PGJ2), and reversed by GW9662, ... induced by troglitazone through a PPARc-dependent Akt-1 signaling pathway (Fig 3A,B) However, the mechanism by which PPARc agonists reduce Akt-1 levels is not fully understood It is not by suppression ... (10 or 20 lM) for 96 h (F) Inhibition of apoptosis by Ac-DEVD-CHO AO ⁄ EB, apoptosis measured by AO ⁄ EB staining; Hoechst, apoptosis measured by Hoechst 33258 staining Results are means ± SDs...
- 10
- 594
- 0
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... increased by incubation with the protein tyrosine phosphatase inhibitor vanadate (Figs 1B and 5C) By contrast, the phosphorylation of CDK1 on Y15 was increased to a much lesser degree by vana- ... D3-CDK4-p27kip1 holoenzyme in thyroid epithelial cells: activation by TSH, inhibition by TGFbeta, and phosphorylations of its subunits demonstrated by two-dimensional gel electrophoresis Exp Cell Res 291, ... the constituent proteins were analysed by Q-TRAP MS (Applied Biosystems, Foster City, CA, USA) Forty-nine ions were selected from the sample and sequenced by MS ⁄ MS Forty-seven of these were peptides...
- 11
- 456
- 0
Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... febrile response induced by SEC1 is dependent on NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄ 3640 FLAP because these phenomena are attenuated by apocynin and not by ETYA, MK 886, Wort or HIMO ... YC, Tatebe S, Mills GB & Unate H (2002) Induction of human MDR1 gene expression by 2-acetylaminofluorene is mediated by effectors of the phosphoinositide 3-kinase pathway that activate NF-kappaB ... by enzymatic and cellular systems J Biol Chem 273, 2015–2023 67 Costa D, Gomes A, Reis S, Lima JL & Fernandes E (2005) Hydrogen peroxide scavenging activity by nonsteroidal anti-inflammatory drugs...
- 13
- 465
- 0
Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
... mixtures, precipitated DNA was analyzed by 1% agarose gel electrophoresis DNA was visualized by staining with ethidium bromide Results are represented by negative images Asterisks indicate nucleic ... deproteinized by incubation with 0.5% SDS and 0.5 mgÆmL)1 proteinase K for 15 at 65 °C and subsequently for h at 37 °C for DNA analysis, and were analyzed by 1% agarose gel electrophoresis followed by staining ... gel electrophoresis followed by staining with Coomassie brilliant blue DNA end-joining EJ reactions (20 lL) of linearized pUC18 DNA catalyzed by cellular fractions or by their resultant induced...
- 13
- 458
- 0
Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf
... anti-NGF) Similar results were obtained by transfecting two human pancreatic tumor cell lines, Ger and MIA PaCa-2, suggesting that the apoptotic response mediated by aggregating anti-Ras scFvs is not ... in the gel, as revealed by Coomassie blue staining (not shown) and by anti-HP1b immunoblotting (Fig 3B,C) Transfection efficiency of scFv–GFP constructs was controlled by anti-GFP immunoblotting ... centrifuged for (15 000 g) at room temperature, boiled for min, and analysed by SDS/PAGE Western blotting was carried out by using the mouse monoclonal anti-myc IgG 9E10 One-twentieth of the original...
- 9
- 624
- 0
Báo cáo khoa học: Modeling of ATP–ADP steady-state exchange rate mediated by the adenine nucleotide translocase in isolated mitochondria potx
... ATPADP steady-state exchange rate mediated by ANT with DWm Plot of ATPADP exchange rate mediated by ANT versus DWm in liver mitochondria depolarized to various voltages by different amounts of SF 6847, ... measured value of the v ATPADP steady-state exchange rate mediated by ANT, and vi is the value of the ATPADP steady-state exchange rate mediated by ANT calculated on the model at a point corresponding ... Titration of ATPADP steady-state exchange rate mediated by ANT with cATR and correlation with DWm (A) ATPADP steadystate exchange rate mediated by ANT determined as a function of cATR concentration...
- 14
- 444
- 0
Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx
... exert cytotoxic effects by generating reactive oxygen species (ROS), generated by Fenton-like reactions and ⁄ or during intracellular reduction of V(V) to V(IV), mainly by NADPH, that contribute ... that induction of HaCaT cell apoptosis by VOSO4 was promoted through the induction of both c-fos and nCLU, the expression of which was affected by Bcl-2 A Fos -mediated vanadium-induced apoptosis ... apoptosis mediated by low substratum rigidity J Biol Chem 282, 752–763 Criswell T, Klokov D, Beman M, Lavik JP & Boothman DA (2003) Repression of IR-inducible clusterin expression by the p53...
- 16
- 312
- 0
Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
... DT40 cell line mediated by PMA/ionomycin treatment, but also that their own transcription is not influenced by PMA/ionomycin Therefore, the apoptotic cell death of DT40 mediated by PMA/ionomycin ... mRNA level was decreased gradually by PMA/ionomycin treatment by 24 h, and the caspase-10 mRNA level was increased by h and thereafter decreased dramatically by 24 h Expression of caspase-3 and ... caspase-8 gene expression by PMA/ionomycin treatment in E2A-/To further clarify the molecular mechanism linked to the apoptotic induction of the DT40 cell line cooperatively mediated by E2A and BCR signaling,...
- 11
- 349
- 0
Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx
... RhoA gene by TGFb1 was observed in HepG2 cells (Fig 1C, middle panel) Overexpression of the Smad3 protein via adenovirus -mediated gene transfer induced transcription of the human RhoB gene By using ... enhanced by overexpression of Smad3 and Smad4 proteins (3.4-fold) and to a lesser extent by overexpression of Smad2 and Smad4 proteins (2.3-fold), and this activity was stimulated further by simultaneous ... combined data in Figs and indicate that transcriptional activation of the RhoB gene by TGFb is mediated, at least in part, by TGFb- FEBS Journal 275 (2008) 4074–4087 ª 2008 The Authors Journal compilation...
- 14
- 420
- 0
Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... box in the upstream region of SCO2780, and its regulation is probably mediated by a cascade mechanism, rather than by direct interaction of DmdR1 On the other hand, the viuB gene (SCO2781) was ... consensus iron box in its promoter region, suggesting that SCO2780 is controlled by indirect iron regulation, probably mediated by a cascade mechanism The desA promoter of S pilosus showed higher expression ... biosynthetic pathway in which cadaverine formed by lysine decarboxylation is subsequently hydroxylated to N-hydroxycadaverine by the protein encoded by desB (Fig 1) which is later FEBS Journal 274...
- 13
- 456
- 0
Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... were generated by in vitro transcription with T3 or Sp6 polymerases RNA was quantified by absorbance at 260 nm and its integrity was verified by agarose gel electrophoresis followed by ethidium bromide ... AREs are known to mediate mRNA degradation by Fig Interferon-b production by Hec-1B cells infected by Sendaı¨ virus Hec-1B cells were infected for h by the Sendaı¨ virus, the cells were then washed ... Winstall, E., Gamache, M & Raymond, V (1995) Rapid mRNA degradation mediated by the c-fos 3¢ AU-rich element and that mediated by the granulocyte-macrophage colony-stimulating factor 3¢ AU-rich...
- 8
- 361
- 0
Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc
... was able to bind speci®cally (Fig 3D, lane 3) and could be supershifted by an Ets-1 speci®c antibody (lane 2), but not by an unrelated antibody (lane 13) Increasing amounts (10 and 100-fold molar ... 12, 1043±1053 Lim, F., Kraut, N., Framptom, J & Graf, T (1992) DNA binding by c-Ets-1, but not v-Ets, is repressed by an intramolecular mechanism EMBO J 11, 643±652 Jorcyk, C.L., Watson, D.K., ... using both PCR fragments with arti®cial restriction sites at the ends and DNA fragments obtained by restriction digestion The oligos used for point mutations in the three ETS response elements...
- 9
- 360
- 0
Báo cáo khoa học: A large complex mediated by Moc1, Moc2 and Cpc2 regulates sexual differentiation in fission yeast ppt
... summary of the interactions between Moc proteins, Cpc2 and Rpl32-2 The interactions detected by coimmunoprecipitation experiments are shown by solid arrows The interactions detected by the yeast ... Table Interaction of Moc3 interacting proteins with other Moc proteins A positive signal is indicated by ‘+’ and a negative signal by ‘)’ The strength of blue color on the X-gal filter is shown by ... Table Interaction of Moc4 interacting proteins with other Moc proteins A positive signal is indicated by ‘+’ and a negative signal by ‘)’ The strength of blue color on the X-gal filter is shown by...
- 18
- 383
- 0
Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot
... is hyperphosphorylated by the protein kinase p38c [3] Therefore, we initiated experiments to determine whether hDlg degradation is affected by phosphorylation mediated by p38c in a cell density-dependent ... low because of its degradation by proteasomes (Fig 2D) [19] These results indicate that the degradation of hDlg upon cellular stress is mediated by caspases and not by the proteasome In addition, ... shock-induced degradation of hDlg is mediated by caspase Osmotic shock causes a decrease of hDlg protein As mentioned previously, hDlg degradation seems to be regulated by phosphorylation and cell density...
- 14
- 360
- 0
Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... H+-ATPase, as measured by its ATP-hydrolyzing capacity, reaching a plateau at 150 lm (Fig 1A) At this concentration, HAT was decreased by 80% Activation of plasma H+-ATPase by d-glucose is a very ... is formed suggests direct interaction of tubulin with ATPase, we cannot rule out the possibility that other molecules mediate this interaction Involvement of glucose transporters is a reasonable ... controlled by the promoter binding factor TUF J Biol Chem 264, 7437– 7446 Eraso P & Portillo F (1994) Molecular mechanism of regulation of yeast plasma membrane H(+)-ATPase by glucose Interaction...
- 11
- 299
- 0
Báo cáo khoa học: Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles pdf
... here only by ATP hydrolysis and which presumably became dramatically low after IF1 addition The fact that an almost complete inhibition of ATPase by IF1 in the presence of D~Hþ is followed by an ... and FCCP separated by Fig 2B shows the time course of ATP hydrolysis by coupled SMP to which IF1 was previously added, as above, with focusing on the last step of reactivation by FCCP Curves and ... hydrolysis initiated by addition of SMP, inhibited by IF1 at two different concentrations, and further reactivated with FCCP Additions (SMP 18 lg, IF1, FCCP lM) are indicated by arrows Curves and...
- 8
- 293
- 0
Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx
... S2¢–P2¢ interaction was further evidenced by the variations in the kcat/Km values for the hydrolysis of various substrates with modifications at P2¢ position by rH84Y – changing the Ser favoured by ... was monitored by absorbance at 220 nm and by fluorescence at 420 nm after excitation at 320 nm The Abz-containing fragments were compared with authentic synthetic sequences and/or by amino acid ... were monitored by their absorbance at 220 nm and by fluorescence emission at 420 nm following excitation at 320 nm The molecular mass and purity of synthesized peptides were checked by MALDI-TOF...
- 11
- 543
- 0
Understanding Tuberculosis – New Approaches to Fighting Against Drug Resistance Edited by Pere-Joan Cardona potx
... Fighting Against Drug Resistance, Edited by Pere-Joan Cardona p cm ISBN 978-953-307-948-6 Contents Preface IX Part Chapter Strategies for New Drug Discovering Multi -Drug/ Extensively Drug Resistant ... fast acting diagnostic tools, drugs and vaccines 1.2 Drug- resistant TB Drug- resistant TB is a result of mycobacterial strains that not respond to drug treatment Drug resistance has in the recent ... of developing one TB drug regimen capable of treating both drug- sensitive and multidrug- and extensively drugresistant tuberculosis using combination therapy Combination drug regimen may especially...
- 386
- 369
- 0
Enhancing Interaction Spaces by Social Media for the Elderly: A Workshop Report pdf
... display and interact with it, e.g., by moving virtual objects around Interaction can be determined by touch-sensitive surfaces or through gesture recognition by video analysis Because of the closeness, ... wellbeing of the elderly by means of focusing on social media By bringing together CSCW and social media researchers we wish to open up discussions on the development of new interaction and coordination ... seniors Against the background of attempting to enhance the social interaction space for the elderly by means of social media, by converging physical and virtual spaces, ethnographically-informed...
- 62
- 737
- 0
Báo cáo khoa học: The activity of Plasmodium falciparum arginase is mediated by a novel inter-monomer salt-bridge between Glu295–Arg404 doc
... two interactions are broken between chain A and B, and chain B and C by the end of 20 ns The third interaction was transiently broken (chain C and A) In the NPT ensemble, only one of these interactions ... structure, the Asp199x cognate forms an inter-monomer bridge with Glu256y that is mediated either by urea or by free arginine, depending on the crystallization conditions [36] 3520 Before proceeding ... Arg346x–Glu347y interaction is susceptible to removal of Mg2+, even though the interaction remained intact As described above, visual inspection of the homology models suggested a further interaction...
- 14
- 425
- 0
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25