driven flexible machine learning strategy for the classification of biomedical data

gene therapy for liver disease

Ngày tải lên : 10/04/2014, 22:11

... is the only curative therapy for HCC However, due to the extent of the tumor and associated cirrhosis at the time of diagnosis, it is inappropriate in the majority of patients The search for ... macrophages nor the depletion of CD4 or CD8 T lymphocytes nor a sublethal dose of whole-body radiation before the injection of the tumor cells prevented the effect of TNF-a On the other hand, the method ... expression of mediators of pathogensis is an important approach that can be utilized in the use of gene therapy of the liver SUMMARY The liver synthesizes a large variety of proteins and, therefore,...

29
256
0

Báo cáo sinh học: "AAV-mediated gene therapy for metabolic diseases: dosage and reapplication studies in the molybdenum cofactor deficiency model" pps

Ngày tải lên : 14/08/2014, 19:22

... response is the time point of viral vector administration The group of Petry et al [19] showed that the efficacy of readministration is dependent on the titer of nAB and that the level of nABs is ... transmission of the vector genome To estimate the necessary dosage for the treatment of humans, we determined the minimal dosage required to rescue the deficiency phenotype via the intravenous ... line) All animals of this group were mated and all but one were fertile Again, the offspring died within the range of untreated animals (data not shown) The observed high variance of the life span...

7
252
0

Báo cáo khoa học: Active-site-speciﬁc chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx

Ngày tải lên : 30/03/2014, 03:20

... : 40 000 for the classic form The incidence of the variant form of Fabry disease was found to be higher Screening of various ethnic groups revealed that the incidence of cardiac variant Fabry ... progress in the development of ASSC therapy for Fabry disease Particularly, 1-deoxygalactonojirimycin (DGJ) is explored as an example of the development of ASSC therapy Structural basis of Fabry ... [28] The ﬁrst domain contains the active-site formed by the C-terminal ends of the b strands at the center of a barrel Thirteen amino acid residues were predicted to be directly involved in the...

10
548
0

gene therapy for hematological disorders

Ngày tải lên : 10/04/2014, 22:10

... TARGETS FOR GENE THERAPY The concept of genetic correction or modiﬁcation of HSCs has been an ongoing primary focus of gene therapy research The properties of both self-renewal and differentiation of ... division The mechanism for this property is not fully understood But, the dissection of the HIV genome and incorporation of the nuclear transport mechanism(s) into otherwise standard MuLV vectors for ... limitations to the use of MuLV vectors for hematopoietic stem cell transduction First, cells must pass through the mitotic phase of the cell cycle in order for the vector to gain access to the chromatin...

20
339
0

gene therapy in cardiovascular disease

Ngày tải lên : 10/04/2014, 22:11

... characterized Furthermore, it has been hypothesized that one can enhance the function of these endothelial cells via the transfer of genes prior to seeding of the cells on the graft surface The initial ... with the increased safety of ex vivo transfection For gene therapy of the heart, genetic manipulation of the myocardium has generated considerable interest in the use of gene transfer of the b-adrenergic ... normalization of myocardial function than either therapy alone factors or the genes encoding these factors have been administered to a small number of patients These studies have involved either the use of...

19
337
0

gene therapy for hiv infection

Ngày tải lên : 10/04/2014, 22:11

... to produce a therapeutic beneﬁt for the patient Therefore, AIDS may be amenable to treatment by gene therapy approaches to inhibit the replication of HIV-1 The ultimate goal of gene therapy is ... pattern of HIV-1 .The diagram depicts the organization of the nine predominant genes of HIV-1 The diagram represents the major RNAs derived from the HIV-1 genome by alternative splicing of the HIV-1 ... with the use of DNA oligonucleotides is that the gene inhibition that is observed is often nonspeciﬁc In other words, the inhibition of expression is most often not the direct result of the interaction...

28
325
0

Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps

Ngày tải lên : 08/08/2014, 21:20

... recoveries in the range of to million per gram of lung tissue The CD11c+ cells were then seeded into 12-well plates at 105 cells per well and cultured for 48 hours in the presence of 10 mg of chitosanpVAX ... 1C); therefore, the decrease in IFN-c production observed in the CD8+ T cells does not appear to be the result of destruction of the cells but represents an IFN-cmediated downregulation of IFN-c ... FACScan gated for CD11c cells The figure shows the percentage of cells that were positive for CD11c and for each of the markers Activation marker expression in CD11c+ cells from the bronchoalveolar...

11
486
0

Báo cáo y học: " Cell therapy for autoimmune diseases" ppt

Ngày tải lên : 09/08/2014, 10:20

... Cell therapies for AD have seen a dramatic development during the past 10 years, especially with the successful use of HSCT for otherwise untreatable forms of AD The recent identification of cell ... HSCT in another patient also resulted in a remission of RA [33] Allogeneic HSCT offers the theoretical benefit of replacing the autoaggressive immune system and utilizing the hypothesized ‘graft ... HSCT in AD The first of these, the ASTIS trial [21], started in 2001 under the auspices of the EBMT/ EULAR to compare the safety and efficacy of HSCT versus conventional pulse therapy cyclophosphamide...

9
359
0

báo cáo khoa học: "Reconsidering low-dose aspirin therapy for cardiovascular disease: a study protocol for physician and patient behavioral change" pot

Ngày tải lên : 10/08/2014, 11:20

... participated in the design of the patient activation form and helped to draft the manuscript WP participated in the design of the study and the patient activation form and helped to draft the manuscript ... clinicians of a one-page information sheet concerning the lack of evidence for low-dose aspirin for primary ASA = Low-dose aspirin Figure CONSORT diagram of the study Page of prevention of cardiovascular ... dartnet.info/media/LowDoseASAEvidenceSummaryforClinicians.pdf) are available online The null hypotheses for the project, stated as null hypotheses are: There will be no significantly different decrease in the use of low-dose aspirin therapy...

6
292
0

Báo cáo y học: " Taking stock of gene therapy for cystic fibrosis" pdf

Ngày tải lên : 12/08/2014, 18:20

... as the first in a recent trial of multiple applications of liposome-mediated gene therapy to the nasal epithelium of CF patients [28] The repeated administration of viral vectors results in the ... about the execution and design of trials in the paediatric population are likely to become the focus of new efforts [29] The rigorous measurement of gene transfer efficiency in vivo and the development ... addressing The key problem of efficiency can be considered in terms of either improving vectors or overcoming biological barriers An intrinsic function of the lining epithelium of the airways...

4
266
0

Báo cáo sinh học: "Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment" pot

Ngày tải lên : 14/08/2014, 19:22

... respectively for the μg, the μg and the μg groups, were reached two months after the first electrotransfer (fig 1) Therefore each dose led to polycythemia which was stronger for the μg and μg ... use in each group the same dose used for the first treatment (i.e μg or 1.5 μg, respectively, for the two treated groups); treatments were performed when the mean hematocrit of the highest dose ... potentially lethal polycythemia [6] Therefore, in the particular case of Epo, an accurate level of transgene expression is required for safety reasons Temporal control systems of transgene expression...

6
233
0

The natural history of non-alcoholic fatty liver disease in children: a follow-up study for up to 20 years pptx

Ngày tải lên : 22/03/2014, 10:20

... population of same age and sex regardless of the causes of death The study also provides interesting data regarding the progressive potential of NAFLD to more advanced disease Four of the five ... components in the development of NAFLD.42 The main strengths of our study are the inclusion of children with the whole spectrum of NAFLD from simple steatosis to cirrhosis along with the long-term ... detailed.32 We used the relationship between the logrank test and the Poisson distribution The p value calculated (from the one-sample log-rank test) depends on the assumption that the number of deaths...

7
487
0

Gene Transfer Approaches for Gynecological Diseases pot

Ngày tải lên : 22/03/2014, 11:20

... in the formation of hemorrhagic ascites and a reduction in microvessel density Virotherapy Utilization of the oncolytic potential of viruses for killing of tumor cells predates the concept of ... into the adenovirus genome to control the expression of the early genes such as E1A, which is essential for viral replication The utility of these agents is subservient to the identification of ... gene therapy Antiangiogenic gene therapy Virotherapy Virotherapy Measles virus Virotherapy Measles and mumps viruses Virotherapy Adenovirus AAV Delivery of p73 HSV-TK suicide gene therapy Virotherapy...

10
348
0

Obesity and Air Pollution: Global Risk Factors for Pediatric Non-alco- holic Fatty Liver Disease potx

Ngày tải lên : 29/03/2014, 18:20

... that of the renal cortex and spleen, with attenuation of the ultrasound wave, loss of delineation of the diaphragm, and poor demarcation of the intrahepatic architecture (37, 38) Prevalence of ... suggested that in spite of the existing association of NAFLD with the early onset of the metabolic and vascular pathogenic changes of atherosclerosis, the evidence for the relationship between ... Determination of the prevalence of NAFLD accurately in children is difficult Because of the aforementioned limitations and controversies in the diagnosis of NAFLD in children and adolescents, data based...

9
552
0

nonviral vectors for gene therapy, methods and protocols

Ngày tải lên : 11/04/2014, 09:59

... ppm) of the N-acetyl groups of the HA-grafts to -methylene protons (3.0 ppm) of the PLL backbone, the content (wt % and grafting-%) of HA in the copolymer was determined The results of the synthesis ... is the molecular weight at the peak of the GPC curve and is therefore independent of the peak integration Therefore, it is useful to compare different batches of polymer Degas the solution in the ... of the resulting complexes and to increase the solubility of the complexes Furthermore, the grafting degree of the copolymer affects the DNA conformation in the complex, allowing regulation of...

381
360
0

viral vectors for gene therapy

Ngày tải lên : 11/04/2014, 10:39

... forming the viral envelope The newly synthesized virions then pass through the ER and cytoplasm and into the extracellular space The ﬁrst newly formed capsids appear in the nucleus within h of ... HSV vector for gene transfer A detailed discussion of the many applications of these vectors is beyond the scope of this chapter We have, however, shown the efﬁcacy and safety of these vectors ... Genome shutdown of host protein synthesis The importance of this observation is that nonessential genes may be deleted in the generation of gene therapy vectors, allowing the insertion of exogenous...

606
386
0

Báo cáo hóa học: "Anti-viral state segregates two molecular phenotypes of pancreatic adenocarcinoma: potential relevance for adenoviral gene therapy" pdf

Ngày tải lên : 18/06/2014, 16:20

... consisting in the induction of type I IFNs followed by the transcriptional activation of hundreds of IFN-stimulated genes (ISGs) [12] In turn, the activation of ISGs promotes the rapid expression of proteins ... diluted in 250 μl of Opti-MEM I, then added into the lipofectamine mixture and incubated for an additional 20 The lipofectamine/DNA mixture was added to each well, incubated at 37°C for h and aspirated ... all genes of potential relevance for pathways analysis [25,26] To verify the relevance of the gene selection in spite of the low significance threshold a permutation test [27,28] was performed following...

11
512
0

Báo cáo hóa học: " Abstract Despite new additions to the standard of care therapy for high grade primary malignant brain tumors, the prognosis for patients with this disease is still poor." ppt

Ngày tải lên : 18/06/2014, 16:20

... that the overall number of open trials is encouragingly higher However, while the number of cellular therapy trials remained the same, the clear trend was towards an increase in the number of vaccine ... and quality of life to become integrated into the standard of care regime [5,52-54] The number of slots open for patient accrual to the immunotherapy protocols contained in our list of open trials ... outlined the direction of, and drafted the manuscript MJH, CCM and KLE refined the search for information, gathered references and generated the tables and figure MRJ, RMP, LML provided information...

10
589
0

Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc

Ngày tải lên : 18/06/2014, 19:20

... length of the amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for ... vitro for testing including phagocytic, epithelial, endothelial and various cancer cell lines [20] Therefore, besides the renal cancer cell OS-RC-2, we further observed the toxicity of the three ... cross-linker [19] The folic acid-coupled PEAs were prepared by the reaction of the activated folate ester with the amine group on the PEAs Evaluation of cytotoxicity of three PEI-derived materials The cytotoxicity...

10
453
0

Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc

Ngày tải lên : 18/06/2014, 22:20

... review the data up to day 30 for each dosing cohort and will then give written recommendation on whether or not to continue the study Members of the committee will also recommend on whether the ... al: Guidelines on the diagnosis and management of acute pulmonary embolism: the Task Force for the Diagnosis and Management of Acute Pulmonary Embolism of the European Society of Cardiology (ESC) ... effects of organ transplantation with chronic immunosuppressive therapy, however, are clinically significant and limit the overall success of the procedure [3-11] Therefore, the objective of this...

10
451
0