do we have right strategies for prevention
Modern Practices in Radiation Therapy Edited by Gopishankar Natanasabapathi pdf
... rationale for the choice of the TomoTherapy platform The TomoTherapy platform has been chosen for the STAT RAD workflow for a variety of reasons TomoTherapy delivers highly conformal and homogenous dose ... allows for increased dose per fraction and fewer total fractions with less toxicity compared to standard non-conformal palliative regimens See Figures 1-2 Fig Nonconformal Technique Fig Conformal ... accomplished with multiple coplanar conformal beams or arcs to allow for a high degree of dose conformality The uniformity requirement is +10%/-5% of the total dose at the prescription point within...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
... Valencia, California, USA) and then cloned into pCR2.1 vector (Life Technologies) Transformants (10 to 25 colonies) were Page of randomly selected and the cloned gene fragments were sequenced ... product sequencing were performed For sequencing, PCR reactions were conducted without SYBR Green I using cDNA generated from cultures stimulated CD3-specific mAb PCR products were purified using a ... (Applied Biosystems) Standard curves were generated for each gene to evaluate primer efficiency and for data analysis Concentrations of purified PCR products were determined by measuring the absorbance...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... standard curves for S Typhimurium on FAM and JOE were 3.37 and 3.37, respectively The values for S Enteritidis were 3.16 on FAM and 3.27 on Cy5 Therefore, the regression curves for S Typhimurium ... meat samples For this purpose, various conditions for the assay were optimized, bacterial DNA were amplified using three sets of primer pairs, and the different amplified products were visualized ... 2.61 ± 0.05 log10 CFU/ ml for S Typhimurium and 0.65 ± 0.07 to 2.66 ± 0.05 log10 CFU/ml for S Enteritidis) were spiked into beef and pork The meat samples were taken for the multiplex real-time...
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Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
... enzyme, 250 nmol/L primers for WEEV and 500 nmol/L for EEEV, 150 nmol/L probes for WEEV and 250 nmol/L for EEEV In the analysis, uL serial diluted transcript RNA of EEEV or WEEV (0.01-106 copies, ... copy/reaction for both EEEV and WEEV Moreover, the performance was linear for at least 106 transcript copies The positive signals only appeared in one default channel, 530 nm for WEEV and 560 nm for EEEV, ... deionized water were added to the final volume of 20 uL PCR was performed with a LightCycler 2.0 (Roche, Switzerland) The reactions were incubated at 50°C for 30 min, followed by 95°C for 10 (inactivation...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
... biofilm sample were used for DNA extraction The samples were homogenized with 450 µL Lysis Solution BB and 50 µL Lysis Solution 20S After homogenizing, the samples were incubated at 65°C for 30 to ... follows: initial denaturation at 95°C for min, followed by 35 cycles of denaturation at 95 °C for 10 s, annealing at 55°C for 10 s, and polymerization at 72°C for 30 s The amplification reactions ... performing the initial denaturation and activation of “hot start” type DNA polymerase at 95°C for 10 This was then followed by 50 cycles of denaturation at 95°C for 10 s Annealing was then performed...
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A real time approach to process control 2ed
... Furthermore, a number of powerful and interactive window environments have been developed for the PC and other inexpensive hardware platforms Windows (2000, NT, XP, etc.), X-Windows, and Mac Systems ... xvi 19:52 Char Count= ENDORSEMENTS FOR THE FIRST EDITION process control because his company “did not have any jobs that made use of Laplace transforms and frequency domain skills” This was an ... electrical systems were, therefore, available when World War II created a massive impetus for weapon controls While the eventual social benefit of this and subsequent military efforts is not without...
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Tài liệu Equal Access Means Equal Opportunity for All: Creating a Real-Time Statewide Advanced Placement Consortium February, 2003 ppt
... guidance counselor that high school students weren't capable of handling college level work, so AP was a bad idea! However, in the summer of 1991 I was fortunate enough to be able to attend a pilot ... courses for the 2003-2004 school year • Conduct an AP Consortium summer training course at UW-Madison and Lawrence University for AP teachers – AP Instructional Content: UW Division for Continuing ... network • Increase the skill levels of Wisconsin’s future workforce 19 Project web page: http://www.cew.wisc.edu/ap_consortium/ AP Consortium web page: http://www.apconsortium.wisc.edu/ 20 ...
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Tài liệu Real-Time Digital Signal Processing - Appendix A: Some Useful Formulas doc
...
A:34 Units of Power Power and energy calculations are important in circuit analysis Power is defined as the time rate of expending or absorbing energy, and can be expressed in the form of a derivative ... SOME USEFUL FORMULAS z1
x1 x2 y1 y2 j
x2 y1 À x1 y2 z2 x2 y2 2 r1 j
y1 Ày2 e : r2
A:19a
A:19b Note that addition and subtraction are straightforward in rectangular form, but ... straightforward in rectangular form, but is difficult in polar form Division is simple in polar form, but is complicated in rectangular form The complex arithmetic of the complex number x can be...
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Tài liệu The Design and Performance of a Real-time CORBA Event Service doc
... organizations contributing to this effort have done excellent work in addressing many of the shortcomings of the CORBA Event Service [22] However, the OMG RFP documents not address the implementation ... distributed object computing standards like OMG CORBA ORBs – we focus on the design Performance, Real-time CORBA and performance of various strategies for implementing QoS in real-time ORBs [5] NETWORK ... quality of service (QoS) enforcement have typically adopted existing solutions from the domain of real-time scheduling, [13], fair queuing in network routers [17], or OS support for continuous media...
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Tài liệu Báo cáo khoa học: "TOWARDS A DICTIONARY SUPPORT ENVIRONMENT FOR REAL TIME PARSING" potx
... Lispreadable format, a powerful virtual m e m o r y system might be able to manage access to the internal Lisp structures resulting from reading the entire dictionary; we have, however, adopted an ... structure containing information relevant to the classification process 176 considerable amount of information in LDOCE which we have not attempted to exploit as yet; for example, the box codes, ... N T S We would like to thank the Longman Group Limited for kindly allowing us access to the LDOCE typesetting tape for research purposes We also thank Karen Sparck Jones and John Tait for their...
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Báo cáo khoa học: Observation of a chaotic multioscillatory metabolic attractor by real-time monitoring of a yeast continuous culture doc
... diagnostic for H2S Although we analyze just one data set here, we have seen this 4-min rhythm repeatedly in this experimental system, often in combination with the circahoralian oscillations We have ... mutual information I(k) between points in the time series separated by k time intervals, i.e between points i and i + k for all values of i The mutual information I(k) measures the amount of information ... i + k we have if we know point i [46] The mutual information curve is shown in Fig The oscillations in the mutual information are due to the strong 4-min mode of the time series: these have a...
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Real-Time Adaptive Security: A SANS Whitepaper – December 2008 pptx
... Shackleford worked as CTO for the Center for Internet Security, as well as for a security consulting firm in Atlanta He has also worked as a security architect, analyst, and manager for several Fortune ... Respond Appropriately Once systems have been given a criticality rating (for example, for mission-critical database servers storing sensitive information and for Web servers with less important ... generated for a series of Windows-focused attacks against DMZ Web servers If these servers are scanned for vulnerabilities to correlate with the alert data, analysts may find that all of the systems have...
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Báo cáo khoa học: "A System for Real-time Twitter Sentiment Analysis of 2012 U.S" pdf
... Therefore, for generating data for model training and testing, we used a crowdsourcing approach to sentiment annotation on in-domain political data To create a baseline sentiment model, we used ... must aggregate sentiment and tweet volume within each time period for each candidate For volume, the system outputs the number of tweets every minute for each candidate For sentiment, the system ... separate publication Here, we focus on presenting the overall system and the visualization dashboards we have built In section 2, we begin with a review of related work; we then turn in section...
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a real time approach to process control
... Furthermore, a number of powerful and interactive window environments have been developed for the PC and other inexpensive hardware platforms Windows (2000, NT, XP, etc.), X-Windows, and Mac Systems ... preference in the area of xvi ENDORSEMENTS FOR THE FIRST EDITION process control because his company “did not have any jobs that made use of Laplace transforms and frequency domain skills” This was an ... electrical systems were, therefore, available when World War II created a massive impetus for weapon controls While the eventual social benefit of this and subsequent military efforts is not without...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
... Statistical analysis All statistical analyses were performed by using SPSS 15.0 software for Windows (SPSS Inc Chicago, IL, USA) Correlations between the expression of different cytokine genes ... titers in healthy donors (n = 29 for a total of n = 39 samples) vaccinated against Hepatitis B virus Samples from naïve, seronegative donors were also studied (n = 9) WB specimens were cultured o/n ... was used as cut-off (dashed lines for IFN-γ and dotted lines for IL-2 gene expression, respectively) WB specimens of the same size (300 μl) from the same donors were simultaneously stained with...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... CD3 and TCR, therefore anti-CD3 staining was not used Fixed cells were shown to have decreased binding as compared to fresh Therefore, freshly thawed, unfixed PBMC were used for validation study ... binding are also shown IFNγ real time PCR assay Freshly thawed HLA-A2 PBMCs at 106 cells/mL/well, duplicate wells in 24-well plate, were cultured for hours at 37°C with 5% CO2 and 95% humidity in serum ... primer for IFNγ or CD8 Samples were stored at -15°C until further analysis Real Time RT-PCR analysis was performed using forward and reverses primer (each at 25 μM) for IFNγ or CD8 The probes were...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... extension at 68°C for The PCR for GII was a denaturation at 94°C for min, followed by 40 cycles at 94°C for 30 s, 48°C for 30 s, 72°C for and a final extension at 72°C for The PCR products were separated ... performed on a MJ PTC-200 thermocycler (MJ research) The reaction was performed for GI with a denaturation step at 94°C for min, followed by 40 cycles at 94°C for min, 45°C for min, 68°C for ... reaction was performed using GII primers with a denaturation step of 94°C for followed by 45 cycles of 94°C for s, 52°C for 10 s, 72°C for 17 s and a fluorescent read step of 84°C for 10 s to melt...
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