dna rna extraction and cdna generation
Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc
... of DRc DNAzyme RNase A cleaved ssRNA and RNase H specifically FEBS Journal 277 (2010) 2543–2549 ª 2010 The Authors Journal compilation ª 2010 FEBS 2547 An RNA- cleaving and DNA- cleaving DNAzyme ... Discussion Design of the DNA- cleaving and RNA- cleaving DNAzyme (DRc DNAzyme) We elected to use an in vitro-selected pistol-like (PL) DNAzyme as a DNA scaffold for designing a DNAzyme with dual catalytic ... conditions FEBS Journal 277 (2010) 2543–2549 ª 2010 The Authors Journal compilation ª 2010 FEBS D Jiang et al An RNA- cleaving and DNA- cleaving DNAzyme A B C D Fig The DNA- cleaving activity and cleavage...
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Lecture 3 DNA RNA and protein synthesis great
... about RNARNA is single-stranded and acts as a code for protein synthesis RNA is still made of nucleotides that have a phosphate, a sugar, and a nitrogen base The sugar is different and the ... ribosomes DNA and protein synthesis, then, happens this way: DNA sequence codes (how letters are put together) produce messenger RNA sequence codes The messenger RNA leaves the nucleus and attaches ... answer is DNA! The species-particular DNA sequences produce the species-particular proteins GENES code for proteins GENES are long strands of DNA on chromosomes What is DNA? DNA is the...
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DNA, RNA and proteins
... the cytoplasm DNA and Protein Synthesis - Translation Translation: 1) rRNA (ribosomal RNA) attaches to mRNA and starts reading the codons 2) tRNA (transfer RNA) – carries amino acids and attaches ... from the DNA to the cytoplasm? mRNA DNA and Protein Synthesis - Transcription Transcription: 1) DNA unzips 2) mRNA (messenger ribonucleic acid) copies info from DNA using base pairings • RNA is ... the ribosome releases the protein chain DNA and Protein Synthesis - Summary DNA and Protein Synthesis Practice making mRNA using the DNA template DNA and Protein Synthesis • Amino acids are linked...
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DNA, RNA, replication, translation, and transcription
... origins and recruit DNA replication machinery proteins • DNA polymerase is responsible for catalyzing synthesis of new strands Replication forks form and involve a leading and a lagging strand • DNA ... Micro RNAs (mRNAs) are important in regulating gene expression • others Transcription involves the synthesis of rRNA from DNA using RNA polymerase • RNA polymerase must unpair and unwind DNA as ... Replication DNA replication is semi-conservative in a daughter strand one strand from each of the initial two strands end up Each strand serves as a template for a new strand New strand is formed...
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Methylation - From DNA, RNA and Histones to Diseases and Treatment by Anica Dricu ppt
... of maternal and paternal genomes during preimplantation development Development 1991: 113, 119-127 15 16 Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones ... them [68-70] Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones to Diseases and Treatment Methylation in carcinogenesis DNA methylation can either affects ... the promoters of indu‐ Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones to Diseases and Treatment cible genes and interacting with transcription initiation...
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Báo cáo y học: "High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing" ppsx
... known as shorthairpin RNAs (shRNAs) shRNAs are in turn processed into short double-stranded -2- pieces of RNA known as short-interfering RNAs (siRNAs) Within the RISC (RNAinduced silencing complex) ... the 2,000 shRNA pool was a subset of the 5,000 shRNA pool and the 5,000 shRNA pool was a subset of the 10,000 shRNA pool, and the set of validated positive and negative control shRNAs described ... RISC: RNA- induced silencing complex; RNAi: RNA interference; RNASeq: RNA sequencing; RRE: Rev Response Element; SDS: Sequence Detection System; shALIGN: shRNA Alignment; shRNA: short hairpin RNA; ...
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Báo cáo y học: "Dissection of a DNA-damage-induced transcriptional network using a combination of microarrays, RNA interference and computational promoter analysis" pot
... preparation and microarray hybridization Cells were treated for h with 200 ng/ml of NCS Total RNA was isolated using TRIzol reagent (Life Technologies) and treated with DNase I (DNA free, Ambion) RNA ... were masked out Both strands were scanned Quantitative real-time RT-PCR reviews Five micrograms of total RNA were used for cDNA synthesis by oligo(dT) and SuperScript II RNase H- reverse transcriptase ... uninfected cells and cells expressing siRNA against the bacterial enzyme LacZ and cells knocked-down for Rel-A, p53 and ATM), each probed at two time points: without treatment and hours after...
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Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
... Typically, distinct bands were observed only for conjugates carrying the longer and more massive 100-base single strand DNA (ssDNA) With shorter ssDNA, such as 18b ssDNA, the distinct bands were not ... controlled Scheme 2.1 Formation of mature miRNA from pri- and pre-miRNA via the actions of Drosha and Dicer, and the RISC complex that regulates mRNA levels [12] MiRNA expression fingerprint was found ... 2.1.1.2 MicroRNA It is known that mRNA is processed before it is used as template for protein synthesis, and this is done through the process of RNA interference Double stranded RNA (dsRNA) known...
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Harvesting, oil extraction, and conversion of local filamentous algae growing in wastewater into biodiesel
... 186 International Journal of Energy and Environment (IJEE), Volume 4, Issue 2, 2013, pp.185-190 treating wastewater and reducing discharge of excess phosphate and nitrate into waterways ... additional extractions ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2013 International Energy & Environment Foundation All rights reserved 188 International Journal of Energy and Environment ... 2076-2895 (Print), ISSN 2076-2909 (Online) ©2013 International Energy & Environment Foundation All rights reserved International Journal of Energy and Environment (IJEE), Volume 4, Issue 2, 2013,...
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Tài liệu EXERGY ANALYSIS AND ENTROPY GENERATION MINIMIZATION docx
... QB, TB and FD are the heat transfer rate, body temperature, and drag force The relation between QB and temperature difference (TB — 7^) depends on body shape and externalfluidand flow, and is ... entropy generation rate, and AT1 and AP refer to the heat transfer and fluid flow irreversibilities, respectively Fig The optimal size of a plate, cylinder and sphere for minimum entropy generation ... Destruction of exergy where £", V, and S are the instantaneous energy, volume, and entropy of the system, and h° is shorthand for the specific enthalpy plus the kinetic and potential energies of each...
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Tài liệu Báo cáo khoa học: Transient DNA ⁄ RNA-protein interactions docx
... rRNA tRNA, E-site tRNA, P-site tRNA, A-site mRNA 30S proteins 30S rRNA Fig Structure of the 70S ribosome The structure of the ribosome of Thermus thermophilus (Protein Data Bank entries 1GIX and ... linker DNA regions in the range of FEBS Journal 278 (2011) 1643–1650 ª 2011 The Authors Journal compilation ª 2011 FEBS Transient protein DNA ⁄ RNA interactions F J Blanco and G Montoya 10–90 bp, and ... features of tRNAGln and tRNAGlu (the second and third bases), whereas the socalled tail domain of the amidotransferase recognizes the outer corner of the tRNAGln (specifically for the tRNAGln) The...
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Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
... Experimental procedures cDNA cloning and DNA constructs cDNAs of the human genes, ER and POLDIP3 (both isoforms) corresponding to their coding sequences (including 4736 the first ATG and stop codons) ... the ER and POLDIP3 genes Array of 76 polyA+ RNA samples isolated from various human adult and fetal tissues and cancer cell lines was hybridized with the radiolabeled ER cDNA probe (A) and after ... generated by subcloning the L7 cDNA insert (with the stop codon) and the ER cDNA (without the first ATG codon and with the stop codon), respectively, into BamHI and NotI sites of pGEX-4T1 (Amersham,...
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Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf
... extraction of attributes from resources such as Wikipedia and top Google snippets In order to test this, we randomly selected 100 object-attribute pairs from our Web QA and WordNet datasets and ... we created a gold standard and compared against it automatically1 For WN enrichment evaluation, our framework discovered size and weight values for 300 WN physical objects, and the quality of ... pre-specified (e.g., (Riloff and Jones, 1999; Chklovski and Pantel, 2004)) and unspecified (e.g., (Banko et al., 2007; Rosenfeld and Feldman, 2007; Davidov and Rappoport, 2008b)) relation types The majority...
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Tài liệu Báo cáo khoa học: "A Mobile Touchable Application for Online Topic Graph Extraction and Exploration of Web Content" ppt
... on such a node expands it, as shown in Fig A single touch on a node that cannot be expanded adds its label to the initial user query and triggers a new search with that expanded query Figure ... one of the extended noun tags2 , and elements of a verb Topic Graph Extraction We consider the extraction of a topic graph as a specific empirical collocation extraction task However, instead ... questions like EEUU and NLF, questions about persons like Justin Bieber, David Beckham, Pete Best, Clark Kent, and Wendy Carlos , and general themes like Brisbane, Balancity, and Adidas The task...
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Tài liệu Báo cáo khoa học: "Integrating Information Extraction and Automatic Hyperlinking" docx
... French, Spanish, Chinese and Japanese allowing for named entity recognition and extraction To guarantee a comparable coverage, and to ease evaluation, an extension of the MUC-7 standard for entities ... as a recognition pattern on the left-hand side, written as a regular expression, and an output description on the right-hand side A named label serves as a handle to the rule Regular expressions ... provides standard operations for constructing and manipulating TFSs; and (iv) an XTDL grammar interpreter Currently, SProUT offers three online components: a tokenizer, a gazetteer, and a morphological...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... total RNA according to the manufacturer’s protocol Double-stranded cDNA was synthesized from the mRNA with a cDNA synthesis kit (TaKaRa, Tokyo, Japan) and used as an abalone cDNA library cDNAs ... of cDNA and genomic fragment for HdEG66 (A) structure of HdEG66 cDNA Open and closed boxes indicate translational and untranslational regions, respectively Relative positions of Hd1 -DNA, Hd2 -DNA, ... of the cDNA library and cloning of cellulase cDNA was achieved as follows: Total RNA was extracted from g of abalone hepatopancreas by the ganidinium thiocyanate-phenol method [30] and mRNA was...
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Tài liệu Báo cáo khoa học: "A HARDWARE ALGORITHM FOR HIGH SPEED MORPHEME EXTRACTION AND ITS IMPLEMENTATION" pptx
... Syntactic and Phonological Rules, Proe llth International Conference on Computational Linguistics: 272-276 Chisvin, L and Duckworth, R J (1989) "Content-Addressable and Associative Memory: Alternatives ... one of the extracted morphemes, is composed of the 2nd candidate at the 1st position, the 1st candidate at the 2nd position and the 3rd candidate at the 3rd position The architecture described ... on Syntactic and Semantic Analysis", Proc 11th International Conference on Computational Linguistics: 280-285 Amble, O and Knuth, D E (1974) "Ordered Hash Tables", The Computer Journal, 17(~):...
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Chủ đề 0. Nhắc lại kiến thức cơ sở của DNA - RNA - Protein (Tải: https://link1s.com/yHqvN)
... (1) Chỉ có DNA cấu tạo theo nguyên tắc đa phân mà đơn phân nucleotit (2) Nucleotit DNA ARN có đường pento, axit photphoric bazo nito (3) DNA có đường C5H10O5 ARN có đường C5H10O4 (4) DNA thường ... nito (3) DNA có đường C5H10O5 ARN có đường C5H10O4 Sai : DNA có đường C5H10O4 ARN C5H10O5 (4) DNA thường có nhiều đơn phân đến hàng triệu ARN có đơn phân có vài chục 10 SĐT : 0902651694 (5) DNA mạch ... nucleotit phân tử DNA Thì chiều dài phân tử DNA virut 10200 Virut có mạch DNA nên chiều dài phân tử 3000.3,4=10200 Vậy đáp án đáp án C Câu Dùng Enzim đặc hiệu E1 , E2 để cắt phân tử DNA thành hoàn...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... CDRs of heavy chain and CDR3 of light chain [ref 39] Fv Randomization in CDR loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local ... proteins In the case of single-chain Fvs and Fabs, artificial libraries of CDR loops have been constructed from synthetic DNA fragments with randomized sequences and lengths The first attempt with artificial ... (XGGGS)n in which the X residues were randomized and the linker length (n) was intermittently varied (B) DARPin: six amino acids in the loop and helix structures are randomized (C) A-domains: variable...
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