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dna protein assemblies floating on supported membranes

báo cáo khoa học:

báo cáo khoa học: " Development-dependent changes in the tight DNA-protein complexes of barley on chromosome and gene level" pot

Báo cáo khoa học

... with covalent or non-covalent bonds after its deproteinisation independently of the deproteinisation method applied: protease digestion, phenol extraction, chloroform extraction or salting-out ... sequences in free DNA and tight DNA- protein complexes along barley chromosomes 1H The distribution of microsatellite sequences in free DNA and tight DNA- protein complexes along barley chromosomes ... the DNA0 fraction [28,29] Graphical presentations of chromosomes 1H and 7H (Figure 6) illustrate the results of amplification on the DNA purified from the different NPC-fractions: DNA 0, DNA...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.

THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.

Y khoa - Dược

... in DNA repair and the DDR we sought to better understand the basic mechanism of DNA- PK activation The two primary components contributing to DNA- PK activation are protein/ protein and protein /DNA ... model of DNA- PK activation being dependent on both protein/ protein interactions and protein /DNA interactions but instead highlights the importance of the N-terminus of DNA- PKcs in activation This ... shown to directly inhibit DNA replication14 During DNA replication Topoisomerase functions to relieve torsional strain in DNA caused by supercoiling, DNA catenation, and DNA knotting15 Etoposide...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES

SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES

Kỹ thuật

... Interactions of Histone Proteins and DNA at Physiological Salt Concentrations Unlike the proteins which are negatively-charged, histone proteins have strong positive charges and thus it can bind to DNA ... stronger hysteresis (c-d) DNA- protein complexes respond to pH in 50 mM KCl and 100 nM protein concentrations Folding is much stronger at lower pH (6.5) and the DNA is hardly unfolded at this condition ... misfolding of histone protein and consequently avoid aggregating into nonfunctional structures during chromosome compaction Histone chaperones play various roles in associating histone proteins Table...
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

Báo cáo khoa học

... mRNA was reverse-transcribed with a Marathon cDNA amplification kit [19,40] The cDNAs were ligated to Marathon adaptors for 5¢ and 3¢ rapid amplification of cDNA ends (RACE), and the products were ... different buffer constituents on the oligomerization of VVA2 Only Triton X-100, and not deoxycholate as had been reported for Bcl-2 family members, was able to induce the oligomerization of VVA2 (supplementary ... that displayed a reasonably high degree of conservation to the VVA2 oligomerization domain Thus, we hypothesized that VVA1 may contain two binding sites for complex formation with VVA2 To address...
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Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

Báo cáo khoa học

... intact protein, the conditions used here will help define solution conditions in which these criteria are met Using chemical shift index (CSI) analysis of the resonances of the intact protein ... the classic 18.5 kDa MBP isoform A key consideration for solution NMR experiments on full-length MBP is the stabilization of secondary, and by extension, tertiary structural elements Although ... conditions is of interest to understand its pharmacokinetics We have initiated solution NMR studies of 18.5 kDa rmMBP to probe its three-dimensional conformation under structure-stabilizing conditions,...
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Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

Báo cáo khoa học

... Indeed, > 16% of mitochondria contained inclusion bodies in 25 cells grown on raffinose, whereas this proportion decreased to < in 20 cells grown on glucose Mitochondrial inclusion bodies were not ... grown on glucose and examined m, mitochondrion; N, nucleus to the heterologous production of mitochondrionaddressed hIRP1 Immunogold electron microscopy of W303-1B producing m-yAco1 and polyclonal ... recombinant protein (lane 1), little staining in the size region of hIRP1 was observed in the soluble fractions of mitochondria (lanes 2–4) In contrast, protein fractions separated with higher concentrations...
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Báo cáo Y học: Location of the Bombyx mori 175 kDa cadherin-like protein-binding site on Bacillus thuringiensis Cry1Aa toxin doc

Báo cáo Y học: Location of the Bombyx mori 175 kDa cadherin-like protein-binding site on Bacillus thuringiensis Cry1Aa toxin doc

Báo cáo khoa học

... Smith-Gill SJ (1990) Epitopes on protein antigens: misconceptions and realities Cell 61, 553–556 48 Jones S & Thornton JM (1996) Principles of protein protein interactions Proc Natl Acad Sci USA ... immobilized on the biosensor surface Bmax, maximum binding amount; kass, association rate constant; kdis, dissociation rate constant; KD, dissociation equilibrium constant; NAM, single Cys-substitution ... concentration of lm, and the association was observed for (binding response) Subsequently, each toxin construct solution was replaced with protein- free binding buffer (NaCl ⁄ Pi), and the dissociation...
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Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc

Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc

Báo cáo khoa học

... extract Comparison of the optimum concentration conditions between the wild-type and K42T systems We analyzed the dependence of protein productivity on the Mg2+ and DNA concentrations for the wild-type ... The condensed S30 is highly productive in cell-free protein synthesis augmented with a dialysis system [23] The translation components, the reaction conditions, and the generation and the consumption ... synthesized the CAT protein efficiently, even in 16.1 mM Mg2+ The optimum concentration of DNA was ngÆlL)1 (Fig 4B) for both systems, and higher DNA concentrations caused decreased protein synthesis...
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Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt

Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt

Báo cáo khoa học

... classification, except the mitochondrial DNA insertion isochore in chromosome II, all other regions in A thaliana belong to GC-poor families and most of the variation between two adjacent regions is ... distributions of the purine ⁄ pyrimidine, amino ⁄ keto and weak H-bond ⁄ strong H-bond along the sequence, respectively As mentioned above, zn displays the distribution of bases of GC ⁄ AT types along ... regions located in chromosome II, which show different properties from other regions The first GC-rich region is located in the nucleolar organizer region (NOR) The second region is a mitochondrial...
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dna–protein interactions principles and protocols second edition - tom moss

dna–protein interactions principles and protocols second edition - tom moss

Sinh học

... footprinting reaction is done in three stages: binding of the protein to the DNA, partial digestion of the proteinDNA complex with DNase I, and separation of the digestion fragments on a DNA sequencing ... gel fractionation and subsequent protein renaturation (see ref 30 and Note 1) Purified or recombinant proteins give valuable information on protein interactions, competition, dimerization or cooperativity ... Resonance Applied to DNAProtein Complexes Malcolm Buckle 535 37 Reconstitution of ProteinDNA Complexes for Crystallization Rachel M Conlin and Raymond S Brown 547 38 Two-Dimensional...
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dna protein interactions

dna protein interactions

Sinh học

... footprinting reaction is done in three stages: binding of the protein to the DNA, partial digestion of the protein -DNA complex with DNase I, and separation of the digestion fragments on a DNA sequencing ... very different DNase concentrations may be required to produce the required degree of digestion on naked and protein- bound DNA A careful titration of the DNase concentration is therefore essential ... fraction and 1M KC1 to bring the final KC1 concentration to 60 mM The maximum volume of the protein fraction that can be used will depend on the salt concentration of this solution The reaction...
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Nghiên cứu sự hấp phụ các phân tử nhỏ lên các phân tử lớn DNA, protein

Nghiên cứu sự hấp phụ các phân tử nhỏ lên các phân tử lớn DNA, protein

Báo cáo khoa học

... k In protein docking calculation these ne ig hb or atoms are belonging In the protein The Newton d a m p algorithm is a search strategy using arid technique to cover all possible initiation position ... owe d that: the configuration data of protein, especially the position of hydr og en atom should be checked seriously before using SQ d ock in g software The active sites on protein are in od ... difference between the docked configuration obtained In MM ami by QM There arc s o m e Q M docked configurations are closer than M M ones and could initiate an electronic transfer process ...
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Copper recorvery and spent etchant regeneration based on supported liquid membrane technology

Copper recorvery and spent etchant regeneration based on supported liquid membrane technology

Cao đẳng - Đại học

... have displaced conventional separation processes such as distillation, absorption, adsorption, solvent exchange, crystallization, etc Compared to other conventional mass separation technologies, ... copper concentration changes in feed solution 107 Figure 4.2: Copper removal rate as a function of carrier concentration 110 Figure 4.3: Influence of the feed Cu(II) concentration on flux ... INTRODUCTION 1.1 General Background Information 1.2 General Information on Membranes 1.3 Membrane Fabrication, Characterization and Evaluation 1.4 Liquid Membranes...
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

Báo cáo khoa học

... environment was most likely affected by the proximity of the DNA, other possibilities can also be envisioned, such as a conformational change in Mhr1 upon DNA binding Mhr1 may interact with Mhr1 on ... defects in mtDNA recombination and in the maintenance of functional mtDNA at 37 °C At 37 °C, respiration-deficient cells grow on yeast extract ⁄ peptone ⁄ dextrose (YPD) plates, but not on YPGly plates, ... mutation defective in mitochondrial recombination in yeast EMBO J 14, 4090–4101 16 Ling F & Shibata T (2004) Mhr1p-dependent concatemeric mitochondrial DNA formation for generating yeast mitochondrial...
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Báo cáo sinh học:

Báo cáo sinh học: " A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and dendritic cells" pot

Báo cáo khoa học

... Figure Activation of adaptive immune response induced by DNA- HSP65 Activation of adaptive immune response induced by DNA- HSP65 (A) Expression of Hsp65 mRNA by monocytes stimulated with DNA vaccine ... immune response than the infection itself It is important to mention that the stimulation induced by DNA vector was as effective as DNA vaccine regarding the cytokine production, expression of surface ... important implications for the design of new strategies based on immunotherapies and, consequently, on modulation of immune response in TB 11 12 13 14 15 Authors' contributions Nine researchers...
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Báo cáo y học:

Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"

Y học thưởng thức

... Discussion This work identifies several lines of evidence indicating that NS1 damages cellular DNA, and that this damage leads to apoptosis Upon detection of DNA damage, DNA damage response proteins ... with DMSO alone (Figure 3B) Inhibition of apoptosis was maximal at 57% at a concentration of 25 µM This finding demonstrates that PARP activation, and therefore the PARP-induced DNA repair pathway, ... initiator protein NS1 and RPA coordinate replication fork progression in a reconstituted DNA replication system J Virol 2002;76(13):6518-31 Young PJ, Jensen KT, Burger LR, Pintel DJ, Lorson CL Minute...
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Báo cáo y học:

Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"

Y học thưởng thức

... real time PCR reactions, demonstrating that there was no gDNA contamination PCR reaction triplicates showed no major differences among them and expression levels of the internal control were similar ... PCR reactions were performed in a volume of 20µl containing the following primer concentrations: ldh (0.3mM -fwd, 0.05mM-rev), csp (0.1mM -fwd, 0.3mM-rev) Each reaction contained 1µl of cDNA template ... multiplication and invasion, suggesting that CSP is likely to be involved indirectly on erythrocyte invasion The immune localisation of the CS-like protein described by Cochrane et al [12] only found...
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 Báo cáo y học:

Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"

Y học thưởng thức

... mutation and maintaining genomic stability It corrects base-base mismatches and short insertions/deletions generated as a consequence of DNA replication errors and homologous recombination [13] ... and incises the newly synthesized DNA strand that contains the mutation [14] Immunohistochemical study on non-polyposis colorectal cancer demonstrated no expression of hMSH2 gene in the tumoral ... H2O2 Sections were counterstained with Meyer’s haematoxylin and mounted in PermountTM Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the...
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Ôn TN12-Chuyên đề - Protein Giải mã protein

Ôn TN12-Chuyên đề - Protein Giải mã protein

Sinh học

... biết đặc biệt roboxom đầu 5’ mARN phía trước đoạn mã hoá cho protein Nhờ anticodon (bộ đối mã) tARN-methionine khở bắt cặp với codon(bộ mã hoá) xuất phát AUG mARN, điểm P (P-site) Sau đơn vị ... dùng chung mã di truyền Trong 64 ba, có ba không mã hoá a.a: UAA, UAG, UGA - ba kết thúc Bộ ba mở đầu AUG, quy định axit amin metionin (Met) sinh vật nhân thực foomin metionin (f-Met) sinh vật nhân ... phân tử protein hoàn chỉnh Poliriboxom: Trên phân tử mARN thường có số riboxom hoạt động, vị trí khác nhau, tổng hợp nên chuỗi polipeptit giống Nhờ đó, khoảng thời gian ngắn, lượng lớn protein...
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Reduction of nitrate by nanoscale zero-valent iron supported on mesoporous silica beads

Reduction of nitrate by nanoscale zero-valent iron supported on mesoporous silica beads

Môi trường

... Journal of Water and Environment Technology, Vol 6, No.1, 2008 These reactions are known to be favorable only in acidic condition of solution (pH
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