dna protein and dna nanoparticle conjugates
Báo cáo hóa học: " A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA" ppt
... http://www.nanoscalereslett.com/content/6/1/461 Page of Modification and coupling of superparamagnetic nanoparticle Determine of cutoff value and validation of QDs and superparamagnetic nanoparticle- based hybridization 3-Aminopropyl-trimethoxysilane ... superparamagnetic nanoparticlebased hybridization with type-specific PCR The 160 outpatients’ DNA samples were checked with QDs and superparamagnetic nanoparticle- based hybridization and type-specific ... Ten HPV-16-negative and ten HPV-16-positive human DNA samples were kept in the clinical laboratory of our department QIAamp® DNA Blood Mini Kits (Qiagen) were used to extract DNA according to the...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... fusion protein was present in inclusion body (Fig 2B) Figure Maps of N-terminal His-tagged gAd-GLP-1-A fusion protein and gAd-GLP-1-A fusion protein (A) N-terminal His-tagged gAd-GLP-1-A fusion protein; ... changed every 12h The protein concentration was measured by BCA Protein Assay Kit PEG20000 was used to concentrate the refolded protein Removal of N-terminal His-tag The refolded protein was incubated ... pure target protein were collected and analyzed by SDS–PAGE The purified N-terminal His-tagged gAd-GLP-1-A fusion protein containing 8M urea was then refolded in urea gradient (6, 4, 2, and M) refolding...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... (EMSA) and RdRp assays, and examined the influence of different cis-acting elements on binding of NS5B protein and RNA synthesis in vitro Materials and methods Expression and purification of NS5B proteins ... NS5B protein, other viral proteins are able to bind the 3¢ UTR, e.g the HCV NS3 protein can bind the 3¢ UTR in vitro and displayed a protease and a helicase activity [36] Some cellular proteins ... nucleotide fragment adjacent to the minus-strand 3¢ UTR), –RNAr2 (a random sequence corresponding to positions 2033–2500 of the minus-strand genome) and minus-strand 5¢ UTR Similarly, we found that the...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... hydrolysis, and to assess the nature of the regions being attacked by proteases in the thermally unfolded protein, fragments CP and 2, as well as fragments TP and 2, were further purified by RP-HPLC and ... beta-lactoglobulin and its similarity to plasma retinol-binding protein Nature 324, 383–385 Pervaiz, S & Brew, K (1985) Homology of beta-lactoglobulin, serum retinol-binding protein and protein HC Science ... 37 °C and pH 8.1 and the same amounts of enzyme present in the heated mixtures We found 25% and 35% inhibition of trypsin activity on 0.5 mM BAPA when native BLG was added at 0.075 mM and 0.15...
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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx
... inhibitors and mutations Biochemistry 39, 15484–15492 Darrouzet, E & Daldal, F (2003) Protein protein interactions between cytochrome b and the FeS protein subunits during QH2 oxidation and large-scale ... cytochrome b haem), and a peak at 575 nm, corresponding to oxyflavohemoprotein [13] After a incubation, the cell suspension became anaerobic as a result of respiration Cytochrome c and cytochrome c ... electroblotting ÔPrecision Plus Protein Dual Color standardsÕ (Bio-Rad) (10–250 kDa) were used for estimation of molecular mass Polyclonal antisera against cytochrome c oxidase subunits VI and VIa were kindly...
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Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf
... various proteins in addition to TcDHH1, including heat shock proteins, mRNAbinding proteins, initiation and elongation translation factors, ribosomal proteins and metabolic proteins Abundant proteins, ... surface protein (MASP) and mucin protein families, with others corresponding to several hypothetical and hypothetical conserved proteins We also observed mRNAs corresponding to metabolic proteins, ... that proteins directly or indirectly associated with TcDHH1 include those of diverse function, e.g translation-related factors, cytoskeleton proteins, heat shock proteins and metabolic proteins...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... development and reduce oocyte size, cause a decrease in total ovary protein incorporation, and suppress ovary total protein synthesis [11,17,18] Biological assays using these criteria are nonspecific and ... americanus [9] than to the mandibular organ-inhibiting hormones of the crab Cancer pagarus [10] MeMIH-A and MeMIH-B are non-sex-specific and are expressed in the eyestalks of males and females The expression ... describes the production of recombinant protein and dsRNA for reproductionrelated eyestalk neuropeptide gene, and use of an in vitro explant culture system and an RNAi technique to demonstrate the...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
... (1fi3)-b-Dglucan-binding protein; subsequently, a gene encoding a fibrinogen-like protein, and also one for a cytokine, are strongly expressed Materials and methods Chemicals and enzymes The sources of chemicals and ... d), for h, and then washed and probed with biotin-curdlan (cur), as described in the Materials and methods (1fi3)-b-D-glucan-binding proteins and was therefore termed GLUBPp_SUBDO The protein comprised ... AB024737.1 and TECL5B_TACTR; AB024738.1) (iii) Angiopoietin: angiopoietin and precursors from mouse (AGP1_MOUSE; O08538 and AGP2_MOUSE; O35608), bovine (AGP1_BOVIN; O18920 and AGP2_BOVIN; O77802), and...
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Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
... biochemical and structural characterization of the protein as a representative of the GH72 family Spectroscopic analyses of the purified proteins were performed, and the behaviour of the purified protein ... features of proteins of the GH72 family and demonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p Materials and methods Strains and growth ... Gas1-C103S and Gas1C265S S cerevisiae cells were cultured in batches at 30 °C in SC medium [0.67% (w/v) YNB, 2% (w/v) glucose and the required supplements at 50 mgÆL)1 for amino acids and uracil and...
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Role of C-Reactive Protein and Procalcitonin in Differentiation of Tuberculosis from Bacterial Community Acquired Pneumonia potx
... stains and cultures, two blood cultures, and urinary antigen assays to detect Legionella pneumophila and Streptococcus pneumoniae All participants had a complete physical examination, and blood ... cultures for respiratory secretions and 13 patients Kang YA, et al C-reactive protein and procalcitonin for the diagnosis of tuberculosis 339 Table Clinical and laboratory characteristics of the ... pulmonary tuberculosis and bacterial community-acquired pneumonia for C-reactive protein (CRP) and procalcitonin (PCT) No difference was detected in the discriminative value between CRP and PCT had positive...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... coat protein and RNA has been investigated [5,9,23,24], and amino acid–nucleotide interactions contributing to its stability have been characterized [13,25], but the role of protein protein and protein RNA ... urea, GdnHCl and high pressure on the structure and stability of whole particles (bacteriophage MS2 and VLPs) and assembly-defective coat protein species [coat protein dimers (dlFG) and single-chain ... presence of both DNA and bis-ANS decreased the stability of the protein (Fig 7B) Effects of high temperature on the secondary and tertiary structure of dlFG and sc-dlFG in the absence and presence...
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basic protein and peptide protocols
... for two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively Plastic and glassware used in the assay should be absolutely clean and detergent-free ... rate of sample electrophoresis and also spoil the resolution of protein bands As mentioned earlier, protein bands in this system are often slightly misshapen, and this problem is exacerbated ... deoxycholate to 1.O mL of protein sample b Vortex, and stand at room temperature for 10 c Add 0.1 mL of 72% TCA, vortex, and centrifuge at lOOO-3000g for 30 d Decant the supematant and treat the pellet...
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protein and peptide analysis by mass spectrometry
... procedures generally used m protein chemistry m terms of senstttvtty and acceptance of solvents, detergents, and buffers, They must be able to handle impure samples and complex mixtures Last, ... K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry/Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York 16 Hogeland, K E , Jr and Demzer, M L (1994) ... , membrane proteins), the highly specific quantitation of btologrcally active pepttdes, and studies of noncovalent mteractions between protems or between protems and low-mol-wt hgands, are now...
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Báo cáo sinh học: " Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition potx
... the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV membranes G7L, a phosphoprotein in ... difference, which is attributed to the expression of the A12L protein and its essentiality in virus replication Essentiality of A12L protein and AG/A cleavage in VV replication The sequence alignment ... requirement of the A12L protein and the AG/A site cleavage for viral replication To begin with, A12L protein expression was confirmed by immunoblot analysis with A12L specific bands obtained only...
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Báo cáo sinh học: "Alpha-1 antitrypsin protein and gene therapies decrease autoimmunity and delay arthritis development in mouse model" potx
... inhibition of proteinases to prevent tissue injury and joint destruction Human AAT is well known as a serine proteinase inhibitor (serpin) It inhibits proteinase 3, neutrophil elastase, and cathepsin ... TG, Vandenabeele P, Buurman WA: Functional protection by acute phase proteins alpha(1)-acid glycoprotein and alpha(1)-antitrypsin against ischemia/reperfusion injury by preventing apoptosis and ... construct was produced and packaged as previously described [27] Briefly, this vector carries hAAT cDNA driven by the cytomegalovirus (CMV) enhancer and chicken b-actin promoter and contains AAV2...
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Báo cáo hóa học: " Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition" pdf
... the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV membranes G7L, a phosphoprotein in ... difference, which is attributed to the expression of the A12L protein and its essentiality in virus replication Essentiality of A12L protein and AG/A cleavage in VV replication The sequence alignment ... requirement of the A12L protein and the AG/A site cleavage for viral replication To begin with, A12L protein expression was confirmed by immunoblot analysis with A12L specific bands obtained only...
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Báo cáo hóa học: " Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions" pdf
... interactions between viral DNA and the nuclear shuttle protein (NSP, encoded by ORF BV1), and between a viral DNA- NSP complex and the movement protein (MP, encoded by ORF BC1) There is good in vitro ... nucleus and facilitates nuclear transport of viral DNA [12] which may be analogous to the nuclear localization and/ or shuttling functions performed by begomovirus CPs and/ or NSPs [41,42]; and MSV ... enzymes, and maize genotypes The cloning vectors pBluescriptSK+ (pSK+; Stratagene, La Jolla, CA) and pUC19 (Stratagene), and the RecAEscherichia coli strains DH5α and JM109 were used in all standard...
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Báo cáo hóa học: " Preparation of Ultrafine Fe–Pt Alloy and Au Nanoparticle Colloids by KrF Excimer Laser Solution Photolysis" docx
... by Fe(II)Cp2, and Pt(II)(acac)2 has also been investigated while keeping constant the sum of both metal complex concentrations and PVP ones to 10 mM and 50 mM, respectively For Au nanoparticle ... of peaks attributed to iron and platinum elements on several points of the nanoparticles, alloying of Fe–Pt alloy in the nanoparticles can be confirmed Except carbon and copper from the grids, ... Fe–Pt nanoparticles are found to be 0.5–3 nm Fringes characteristic for crystallinity in nanoparticles were partly observed on the Fe–Pt nanoparticles in Fig 4a Assemblies or aggregations of nanoparticles...
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Báo cáo hóa học: " The Influences of Cell Type and ZnO Nanoparticle Size on Immune Cell Cytotoxicity and Cytokine Induction" pptx
... significant differences were observed between monocytes and NK cells (0.5 mM and mM; p = 0.0002 and p = 0.0008, respectively), and monocytes compared to T and B lymphocytes (p \ 0.0001 for both cell types ... both monocytes and CD4? T cells against cell death (p = 0.0001 and p = 0.0481, respectively), and implicates ROS formation Fig Kinetics and magnitude of ROS production in T cell and monocyte populations ... numbers, and flow cytometry was used to evaluate changes in PI staining and to quantify cell death NPs were excluded from the analysis based on the absence of fluorescence signal and light FS and SSC...
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