dna but does not affect other steps in the hiv 1 life cycle

Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc

Ngày tải lên : 19/02/2014, 07:20

... Protein mRNA Protein mRNA Protein mRNA TA SOL 10 0 + C 13 5 + 13 B 10 0 + 10 B 15 3 + 31 B 457 + 46 A Liver 11 1 + C 10 0 11 3 11 3 12 1 12 7 18 24 10 0 + B 11 3 + B Heart 10 0 10 2 10 4 10 3 10 4 20 14 10 0 13 4 17 2 ... P07 310 P 215 50 Myosin light chain Myosin light chain Myosin light chain Parvalbumin F1-ATPase b-subunit Creatine kinase, M chain b-enolase 18 16 14 12 51 41 46 9 41 642 310 305 080 17 1 858 5.07 4.66 ... for ‘old’ proteins: The role of the calciumbinding proteins calbindin D-28k, calretinin and parvalbumin, in cerebellar physiology Studies with knockout mice Cerebellum 1, 2 41 258 10 6 Gailly P (2002)...

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Báo cáo y học: "Nifedipine decreases sVCAM-1 concentrations and oxidative stress in systemic sclerosis but does not affect the concentrations of vascular endothelial growth factor or its soluble receptor" potx

Ngày tải lên : 09/08/2014, 01:23

... AOPP (µmol/l of chloramine-T equivalents) 75.5 ( 21 91) 10 9 .1 (50–2 81) P < 0.00 01 VEGF (pg/ml) 2 21 (19 –499) 573.5 (26 19 15) P = 0.00 01 sVEGFR -1 (pg/ml) 19 (8–73) 45 (10 11 40) P = 0.0025 Values ... experimental evidence is needed to determine whether the VEGF pathway is intrinsically defective in SSc Competing interests None declared References 10 11 12 13 14 R 314 LeRoy EC: Systemic sclerosis: ... ameliorated by nifedipine is consistent with the hypothesis that VEGF signalling is impaired in SSc Our results also not support the implication of the sVEGFR -1 in the VEGF dysregulation, but more experimental...

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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc

Ngày tải lên : 12/08/2014, 23:23

... 19 90, 18 7:307- 314 Page of doi: 10 .11 86 /17 42-4690-7-40 Cite this article as: Ahn et al., The RNA binding protein HuR does not interact directly with HIV- 1 reverse transcriptase and does not affect ... positions in the full length proteins The putative binding site for HIV- 1 RT is indicated by the arrow at the C-terminus of HuR [9] We used 1H ,15 N-HSQC NMR spectroscopy [19 ,20] to probe whether a ... corresponding to the HIV- 1 sequence of (-) strong stop DNA The 18 nucleotide DNA oligonucleotide primer is complementary to the HIV- 1 tRNALys3 primer binding site in the RNA template The reaction...

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Báo cáo y học: "Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef" ppsx

Ngày tải lên : 13/08/2014, 01:20

... due to the distinct tetherin localization in these cells HIV- 1 replication in iDCs upregulates tetherin independently of Vpu To examine the role of Vpu and tetherin interactions in HIV- 1 infection ... 14 0 0 .1 CD 81 C D mDC-IFNα Marker HIV- GFP-Vpr Merge DIC mDC-IFNα Pearson’s correlation coefficient (r2) LAMP -1 CD 81 0.5 Tetherin LAMP -1 Tetherin 207 13 9 0.4 0.3 19 1 0.2 0 .1 CD 81 LAMP -1 Tetherin ... tetherin affects incoming HIV- 1 captured by mature DCs We sought to investigate the direct effect of tetherin on the replication and DC-mediated transmission of HIV- 1 by silencing tetherin in...

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Security Considerations in the System Development Life Cycle potx

Ngày tải lên : 30/03/2014, 01:20

... common understanding of SDLC, and the discusses the roles and responsibilities involved in integrating information security into the SDLC • Chapter 3, Incorporating Security into the Information System ... security activities into their established system development life cycle guidelines 1. 1 Purpose and Scope The purpose of this guideline is to assist agencies in building security into their IT development ... throughout the life cycle Implementing information security early in the project allows the requirements to mature as needed and in an integrated and cost-effective manner Engineering security into...

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Báo cáo sinh học: " Functional relevance of nonsynonymous mutations in the HIV-1 tat gene within an epidemiologically-linked transmission cohort" pot

Ngày tải lên : 18/06/2014, 18:20

... of the time points (or all of the time points for donor A) within the respective individual but were not seen in any other individual Overall, despite differences in HIV- 1 genetic variability in ... 4 :10 7 http://www.virologyj.com/content/4 /1/ 107 transactivation ability [15 -17 ], and R52 participates in the binding of Tat to TAR and is involved in the nuclear localisation of Tat [18 ,19 ] The ... 63(3) :11 81- 118 7 Kuppuswamy M, Subramanian T, Srinivasan A, Chinnadurai G: Multiple functional domains of Tat, the trans-activator of HIV- 1, defined by mutational analysis Nucleic Acids Res 19 89, 17 (9):35 51- 3561...

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báo cáo hóa học: " Endotoxin-induced cytokine and chemokine expression in the HIV-1 transgenic rat" docx

Ngày tải lên : 19/06/2014, 22:20

... IL -10 rα: Interleukin 10 receptor, alpha; IL -11 : Interleukin 11 ; IL -12 : Interleukin 12 ; IL -13 : Interleukin 13 ; IL -13 r 1: Interleukin 13 receptor alpha 1; IL -15 : Interleukin 15 ; IL -16 : 19 ` Interleukin ... HIV- 1Tg Rat Spleen ` 24 19 .8( 11 .4) 6.6(±6.4) 32.0(±4.9)* 0.8(±0.7) Interleukin 13 IL -13 14 .2(±6 .1) 3.8(±3.0) 22 .1( ±6.0)** 3.7(±2.3) Interleukin 13 IL -13 ` References: 10 11 12 13 14 15 16 17 18 ... IL -1 LL+L 3 61. 1( 18 7.7) 9 61. 8(±89 .1) 12 754.4( 11 613 .3)^ 19 9 51. 8(±4023.8)** 5087 .1( 16 75.4) 11 443 .1( ±7400)*^ SS+L LL+S LL+L 27.3( 12 .6)*^ 7.3(±2.0) 41. 8(±5.7)** 3.8( 1. 4) Interleukin 239.4(±77 .1) ^...

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Báo cáo hóa học: " Functional relevance of nonsynonymous mutations in the HIV-1 tat gene within an epidemiologically-linked transmission cohort" pot

Ngày tải lên : 20/06/2014, 01:20

... of the time points (or all of the time points for donor A) within the respective individual but were not seen in any other individual Overall, despite differences in HIV- 1 genetic variability in ... 4 :10 7 http://www.virologyj.com/content/4 /1/ 107 transactivation ability [15 -17 ], and R52 participates in the binding of Tat to TAR and is involved in the nuclear localisation of Tat [18 ,19 ] The ... 63(3) :11 81- 118 7 Kuppuswamy M, Subramanian T, Srinivasan A, Chinnadurai G: Multiple functional domains of Tat, the trans-activator of HIV- 1, defined by mutational analysis Nucleic Acids Res 19 89, 17 (9):35 51- 3561...

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Báo cáo y học: "Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivi" pps

Ngày tải lên : 13/08/2014, 01:20

... Incorporation (%) WT 10 0 10 0 10 0 10 0 10 0 A 62 10 1 57 17 10 1 B 41 195 16 C 30 12 5 13 D E 31 30 12 0 13 6 17 22 Y 96 447 47 73 YA 62 563 14 15 4 YB 35 396 39 YC 30 563 56 YD 29 495 YE 17 19 4 17 Bhakta et ... containing the WT Y 712 motif and those containing the Y 712 C mutation Direct comparison of the two panels indicates that the Y 712 C mutation did not affect the fusogenicity of the Env mutants in the ... attempted to study the role of the CD in the context of chimeric proteins [4 ,10 ,11 ,39,40], while others have truncated the CD in order to determine the affects on Env functionality [19 , 61- 64] However,...

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Báo cáo y học: " Massively parallel pyrosequencing highlights minority variants in the HIV-1 env quasispecies deriving from lymphomonocyte sub-populations" docx

Ngày tải lên : 13/08/2014, 05:21

... microliter) HIV- RNA T0** (Log10cp/ml) CD4 T1*** (cells/ microliter) HIV- RNA T1*** (Log10cp/ml) Pt .1 48 F 18 312 569

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Báo cáo y học: "Endothelin-1 enhances fibrogenic gene expression, but does not promote DNA synthesis or apoptosis in hepatic stellate cell" doc

Ngày tải lên : 13/08/2014, 13:20

... http://www.comparative-hepatology.com/content/5 /1/ 5 11 12 13 14 15 16 Competing interests The author(s) declare that they have no competing interests 17 18 Authors' contributions MS performed most experiments and wrote the manuscript ... of ET -1 on HSC proliferation ET -1 in 1st passage HSC did not affect DNA synthesis in the presence of 0 .12 5%, 5% or 10 % fetal calf serum (FCS) (Fig 2A), or in the presence of 10 -6 M of BQ123, a ... 0.52% in 4th passage HSC (n = 6) when cultured in 10 % FCS for 24 h Addition of ET1 (10 -10 , 10 -8, 10 -6 M) did not alter the basal level of apoptosis (1. 03%, 1. 32% and 1. 19%, respectively, in 1st...

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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx

Ngày tải lên : 07/03/2014, 16:20

... MC 410 0Dtig MC 410 0Dtig::Cmr MC 410 0DdnaKdnaJ MC 410 0DdnaKdnaJ ::Kanr thr::Tn10 MC 410 0DtigDdnaKdnaJ MC 410 0Dtig::CmrD dnaKdnaJ::Kanr thr::Tn10 MC 410 0DtatA/E MC 410 0DtatADtatE MC 410 0DtatB MC 410 0DtatB MC 410 0DtatC ... pNR30, including the SufI coding region and the ﬁrst 18 bp upstream of the ATG-start codon, was cloned into pBAD18 [19 ] The resulting plasmid pBAD18-SufI was then used as a template in PCR using the ... post-translational targeting and translocation mechanism Work is in progress to elucidate the identity of the and 17 kDa crosslinked partners 10 11 12 13 14 15 16 17 Acknowledgements We are...

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Báo cáo y học: "Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis" pps

Ngày tải lên : 09/08/2014, 07:20

... 16 8.4 (10 .3) 16 4.7 (9 .1) 7 /14 8 /14 7.5 (10 .1) 10 .2 (11 .7) 9 /14 11 /14 3 .1 (3.5) 4.3 (4.0) 10 /14 11 /14 11 .6 (8.2) 8.5 (5.6) Number of painful joints 5 .1 (5 .1) 7.9 (8.9) Number of swollen joints 8.9 ... αEAST 1. 88 (1. 67 1. 99) 1. 85 (1. 64 1. 96) 1. 80 (1. 68 1. 93) 1. 33 (1. 29 1. 40) 0.0 01

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Báo cáo y học: "Tracheotomy does not affect reducing sedation requirements of patients in intensive care – a retrospective study" potx

Ngày tải lên : 12/08/2014, 23:24

... study 11 7 57.6 (46.3– 71. 8) 78 (66.7) 21. 0 (16 .0–26.0) SAPS II score 44.0 (35.0–57.0) ICU mortality In our ICU, as in many institutions in The Netherlands [ 21] , percutaneous tracheotomy is the method ... nine days) than that in the study of Nieszkowska and colleagues (median 14 days) Accordingly, the timing of tracheotomy in our study cannot explain the discrepancy between the two studies Finally, ... interests The authors declare that they have no competing interests Authors' contributions DPV participated in the analysis and interpretation of the data and in drafting the manuscript DAD contributed...

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Báo cáo y học: "Tracheotomy does not affect reducing sedation requirements of patients in intensive care – a retrospective study" ppt

Ngày tải lên : 12/08/2014, 23:24

... study 11 7 57.6 (46.3– 71. 8) 78 (66.7) 21. 0 (16 .0–26.0) SAPS II score 44.0 (35.0–57.0) ICU mortality In our ICU, as in many institutions in The Netherlands [ 21] , percutaneous tracheotomy is the method ... nine days) than that in the study of Nieszkowska and colleagues (median 14 days) Accordingly, the timing of tracheotomy in our study cannot explain the discrepancy between the two studies Finally, ... interests The authors declare that they have no competing interests Authors' contributions DPV participated in the analysis and interpretation of the data and in drafting the manuscript DAD contributed...

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Báo cáo y học: "Activated protein C ameliorates coagulopathy but does not influence outcome in lethal H1N1 influenza: a controlled laboratory study" potx

Ngày tải lên : 13/08/2014, 20:21

... Received: 14 January 2 010 Revised: 25 March 2 010 Accepted: 14 April 2 010 Published: 14 April 2 010 Page of 10 10 11 12 13 14 15 16 17 18 19 20 Criticalan open access articlehttp://ccforum.com/content /14 /2/R65 ... 11 (5.0 to 28) * IL -10 (pg/ml) 13 7 (86 to 16 5) 13 1 (10 1 to 15 3) 11 5 (82 to 17 5) 65 (23 to 14 7) IFN-γ (pg/ml) 6.8 (6.0 to 9.7) 6.9 (5.8 to 7.9) 6.7 (4.9 to 8 .1) 5.6 (3.5 to 7.9) KC (ng/ml) 3 .1 ... these hemostatic derangements, but does not impact on lung inflammation or survival Figure Survival in lethal H1N1 influenza A infection is not affected by recombinant murine activated protein...

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Báo cáo sinh học: "Second chromosome polymorphism of Drosophila buzzatii in a natural population is not associated with gametic selection and does not affect mating pattern" potx

Ngày tải lên : 14/08/2014, 20:20

... tests The remaining individuals in the bottles were maintained until pupation in order to estimate larval viability by counting all pupae Further collections were performed in A Escobar in March 19 89 ... and selected against in another, a pattern called endocyclic selection Moreover, they suggested that this pattern might be involved in the maintenance of polymorphisms However, their conclusions ... pooling the progeny that received 2st versus 2j in all crosses involving the 2st/j heterokaryotype (from table IV), the x value was not significant (x2= 3 .13 , df 1, P > 0.05) Likewise, none of the...

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Báo cáo khoa học: Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration ppt

Ngày tải lên : 22/03/2014, 16:20

... within the ﬁrst 16 amino acids, as their removal results in loss of neuroprotective phenotype [20, 21] Interestingly, the only known binding partner of the N-terminal region, AAA ATPase valosin-containing ... HindIII (10 13 8) BamHI EcoRI (1) (7282) loxP1 7000 NEO loxP2 BamHI (13 390) loxP3 8000 14 000 11 000 BamHI Pr1 Sal1 Sal1 Pr2 Pr3 16 000 17 18 7 HindIII Pr4 15 000 HindIII (18 670) Type II del (BamHI ... and 0.02 mgÆmL )1 each of leupeptin, antipain, chymostatin and pepstatin, FEBS Journal 278 (2 011 ) 2666–2679 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 2675 NMNAT1 gene inactivation and...

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báo cáo hóa học:" Does metformin affect ovarian morphology in patients with polycystic ovary syndrome? A retrospective cross-sectional preliminary analysis" pot

Ngày tải lên : 20/06/2014, 07:20

... 3.45 22.92 ± 1. 51 0.85 ± 0 .11 12 .70 ± 2. 41 5.83 ± 1. 40 12 .65 ± 3. 51 3 .10 ± 0.73 9.52 ± 1. 81 48.80 ± 14 .95 1. 27 ± 0.45 1. 74 ± 0.50 4.70 ± 1. 23 4.59 ± 1. 99 2690. 01 ± 19 5.67 31. 4 ± 1. 78 15 .06 ± 4.35 ... 4.72 ± 0.45 16 .24 ± 3.60 5.59 ± 1. 16 3.32 ± 0. 61 Six months 28.20 ± 3.42 23.84 ± 1. 46 0.83 ± 0 .14 12 .70 ± 2.26 5.82 ± 1. 25 12 .14 ± 1. 52 3 .14 ± 0.49 10 .02 ± 1. 97 48 .18 ± 14 .90 1. 26 ± 0. 31 1.59 ± 0.82 ... ± 1. 11* 4.34 ± 1. 80* 2653.48 ± 12 6.05 35.90 ± 1. 66*° 14 .37 ± 4 .15 * 4.79 ± 0.33 14 .94 ± 2.36*° 6.53 ± 1. 00*° 3.02 ± 0.46*° Ovulatory PCOS (n .10 ) Baseline 28 .10 ± 3. 31 22.93 ± 1. 71 0.84 ± 0 .13 12 .13 ...

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Báo cáo y học: "Systemic TNF blockade does not modulate synovial expression of the pro-inflammatory mediator HMGB1 in rheumatoid arthritis patients – a prospective clinical study" ppt

Ngày tải lên : 09/08/2014, 10:23

... HMGB1 protein 1. 5 4.94 19 6.00 5. 41 15 No 7. 91 16 9 46 ACR 6. 71 4.43 7.5 7.5 34 14 Clinical response DAS28 6.34 25 5* 15 17 .5 38 10 7 DAS28 Pred Up Down Down 3 Up * Patient also received 15 0 mg/day ... of infliximab therapy (data not shown) Synovial HMGB1 protein expression not influenced by infliximab therapy Aberrant synovial HMGB1 staining was evident in all nine patients before and during ... decreased (Figures 1c,d) presence of HMGB1 protein in the synovia during therapy could be detected, but there was no correlation with the clinical course of individual patients In the group of six...

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