dna blunt ending sheared ends ligation of non biotinylated linkers
Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... activities: (a) DNA polymerase activity, which can be both RNA and DNA dependent; and (b) RNase H activity that selectively hydrolyzes the RNA strand of the RNA :DNA hybrid formed during synthesis of the ... incubated in the presence of different concentrations of K-49 and in the absence () or presence of lM (.) or 10 lM ( ) RDS1643 (B) YonetaniTheorell plot of the interaction of K-49 and efavirenz on ... the presence of different concentrations of efavirenz and in the absence () or presence of 1.9 lM (s), 3.7 lM (.) and 7.5 lM (4) K-49 (C) YonetaniTheorell plot of the interaction of KNA-53 and...
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Báo cáo khoa học: Template-independent ligation of single-stranded DNA by T4 DNA ligase doc
... Ligation of ssDNA by T4 DNA Ligase nontemplated ligation on ssDNA substrates [29,30], this property of T4 DNA ligase has, to our knowledge, not been reported Unlike T4 DNA ligase, bacterial DNA ... template-independent ssDNA ligation Oligonucleotide I was incubated with either E coli DNA ligase, Taq DNA ligase, or Ampligase Ligation Ligation of ssDNA by T4 DNA Ligase A B Fig Analysis of amplicons ... investigate the dependence of the efficiency of nontemplated DNA ligation on the strandedness of the DNA substrate at the ligation point, we determined A B Fig Determination of ligation yields by qcPCR...
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The mammalian transcriptome and the function of non-coding DNA sequences pdf
... numerous non- protein-coding genes, as evidenced by the high proportion of non- protein-coding transcripts in human and mouse cDNA libraries [4,6] Estimations of the relative complexity of heterogeneous ... analysis of the mouse transcriptome on the basis of annotation of full-length cDNA collections enabled identification of 33,409 unique full-length transcripts, with an estimated total number of independent ... function of non- coding DNA remains poorly studied, and interspecies comparison is often the only way to demonstrate that a conserved DNA sequence, which has evolved slowly as a result of negative...
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Báo cáo y học: "The Diels-Alder-Reaction with inverse-Electron-Demand, a very efficient versatile Click-Reaction Concept for proper Ligation of variable molecular Partners"
... inside of the cyclobutene ring The presence of a further double bond inside of the cyclohexene ring is not of relevance due to the inactivity of this double bond in the DARinv The anhydride ring of ... Functionalization of tetrazines with temozolomide –TMZ Indeed the situation of the synthesis of a further group of dienes used in our TMZ-BioShuttle studies is unequally catchier and the synthesis of functionalized ... of chemistry harbours a collection of chemical reactions whose mechanisms were identified, characterized and collected during decades, offering promising solutions Ligation methods A series of...
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The translation of health insuracne terms of non-equivalence group
... deletion of OF 26 II.4 Concluding remark 26 CHAPTER III: THE TRANSLATION OF HEALTH INSURANCE TERMS OF NON- EQUIVALENCE GROUP 28 III.1 The source of non- equivalence problem in the translation of health ... employed are the focus of the research Chapter III: The translation of HI terms of non- equivalence group This chapter examines how non- equivalence problem in the translation of HI terms in the US ... deletion of OF Group 3: Terms consisting of Thing + Qualifier (noun + of +noun/noun phrase) 32 Within this study, we have collected 13 nominal groups in the form of ‘Thing + Qualifier (of phrase)’...
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Rethinking of Non-traditional Water Resources in Residential Developments of Rural Towns, Western Australia
... annual number of rain days for Cranbrook is 131 The modeling of this study is based on the average roof area of 249 m2 and 2.4 persons per block (Western Australian Department of Planning and ... parts of integrated water management METHODOLOGY Description of Study Area and Climate Data The township of Cranbrook is located in the Great Southern Region, Western Australia, at coordinates of ... recommend the quantity of toilet flushing covering 21% of all domestic water use Using greywater for toilet flushing only would be marginally necessary because of large volumes of overflow In order...
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Judgements of non-compliance in pregnancy
... interactions The scope of compliance /non- compliance judgements To situate the experience of women who are judged to be non- compliant with respect to obstetrical advice, we review the range of advice that ... beneWts of pre-conceptual folic acid however, many remain unaware of these beneWts And among those who are aware of the beneWts of folic acid supplementation there is still a high rate of incorrect ... testing In the face of public and professional debates about the appropriateness of the genetics agenda, refusal of genetic testing is generally tolerated Also, the label non- compliant is seldom...
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A STUDY ON THE DIFFICULTIES IN STUDYING TOEIC READING OF NON ENGLISH MAJORS AT ELEMENTARY LEVEL AT HAIPHONG PRIVATE UNIVERSITY
... Table of content PART I: INTRODUCTION I RATIONALE OF THE STUDY: II AIM OF THE STUDY: III SCOPE OF THE STUDY: IV METHOD OF THE STUDY: V DESIGN OF ... are part of longer pieces of writing such as a letter Types of TOEIC reading comprehension text 3.1 Letters & emails Sample of letters & emails: 13 3.2 Forms Sample of form: Hawaii Bills of Sale ... half of students say that the method of teacher not interesting 22 % is the number of students says the method of teacher interesting Rank at the third position is the number of says the method of...
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Tài liệu THE MATHEMATICAL THEORY OF NON-UNIFORM GASES pptx
... the light of the work of Kihara, of Waldmann, of Grad and of Hirschfelder, Curtiss and Bird A few minor changes of notation have been made; these are set out at the end of the list of symbols ... the sum of the products of corresponding components of w and w' In particular, w: w is the sum of the squares of the components of w, and U: U = From these definitions it follows that each of the ... direction of finding approximate solutions of the Boltzmann equation in regions having a thickness of the order of a mean free-path These new solutions were, of course, beyond the reach of the methods...
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Tài liệu Report on The Management of Non-Muscle-Invasive Bladder Cancer ppt
... probability of recurrence compared to use of TURBT alone Some of the differences in Table are quite large An example is the use of TURBT plus BCG versus the use of TURBT alone With a median difference of ... number of patients with recurrences, number of recurrences, number of occurrences per unit of time or time of first recurrence For progression, some studies reported patients with different types of ... Carcinoma in situ of the bladder J Urol 1995;153:564-72 Jewett HJ, Strong GH Infiltrating carcinoma of the bladder: relation of depth of penetration of the bladder wall to incidence of local extension...
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Tài liệu Study on the Development and Marketing of Non-Market Forest Products and Services ppt
... Direct/Indirect use, Non- use Direct/Indirect use, Non- use Direct/Indirect use, Non- use Market Non- market Private Public Non- market Non- market Non- market Non- market Non- market Non- market Public Public ... Public Public Non- market Market /Non- market Market /Non- market Market /Non- market Market /Non- market Public Club/Public Club/Public Club/Public Club/Public Market /Non- market Club/Public Non- market Public ... Study on the Development and Marketing of Non- Market Forest Products and Services Table 6.2: List of non- market forest goods and services Remark: The list of non- market forest goods and services...
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Tài liệu The Total Cost of (Non) Ownership of a NoSQL Database Cloud Service ppt
... headaches of provisioning hardware and systems software, setting up and configuring a distributed database cluster, and managing Page of 14 Amazon Web Services – The Total of (Non) Ownership of a ... Page of 14 Amazon Web Services – The Total of (Non) Ownership of a NoSQL Database Cloud Service March 2012 Summary of TCO Analysis When calculating for TCO, you should include the costs of servers ... (High) Month (Medium) 5000 (peak) 2000 (off-peak) 5000 (peak) 2000 (off-peak) 2000 (peak) 1000 (off-peak) 2000 (peak) 1000 (off-peak) 900 1,200 Table 1: Usage Profiles Month 1: In the first month,...
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Tài liệu From Burden to “Best Buys”: Reducing the Economic Impact of Non-Communicable Diseases in Low- and Middle-Income Countries pdf
... the growing burden of NCDs: • A global analysis of the economic impact of NCDs by the World Economic Forum and the Harvard School of Public Health • An analysis of the costs of scaling up a core ... School of Public Health (HSPH) on the economic impact of NCDs was carried out by a team led by Professor David E Bloom (Clarence James Gamble Professor of Economics and Demography, Harvard School of ... Economic Burden of Non- communicable Diseases – Prepared by the World Economic Forum and the Harvard School of Public Health (2011) Source: Based on The Global Economic Burden of Non- communicable...
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Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt
... PolC and DnaE The ability to bind single-stranded DNA has indeed been demonstrated for the E coli DnaE OB domain [12,16] The very N-terminal region of PolC and the C-terminal domain of DnaE appear ... probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of the so-called type II KH fold [21] One of their major differences from classical type II KH domains is the absence of the ... higher similarity to domain I of the initiator of chromosomal replication DnaA (DnaA-I) 3114 What might the function of these PolC N-terminal domains be? The involvement of related structures in protein–protein...
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Đề tài " The L-class of non-Witt spaces " pot
... example of the reductive Borel-Serre compactification of a Hilbert modular surface Contents Introduction The Postnikov system of Lagrangian structures The bordism group ΩSD ∗ The signature of non- Witt ... intersection homology of all links of strata of odd codimension vanishes In [GM83], an elegant formulation of intersection homology theory is presented employing differential complexes of sheaves in the ... compactifications of locally symmetric varieties constitute an interesting class of examples of non- Witt spaces Concretely, the reductive Borel-Serre compactification — see [Zuc82] or [GHM94] — of a Hilbert...
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Taxation of Non-residents in Spain: Non-Resident Income Tax pdf
... for non- resident taxation (BOE of 30 December 2003) • Royal Decree Law 5/2004 of March 2004, which approved the revised text of the Non- Resident Income Tax Law (BOE of 12 March 2004) • Order of ... ESTABLISHMENT (PE) Taxation of non- residents of Spain varies considerably depending on whether or not the non- resident has a permanent establishment in Spain This factor is therefore of special importance ... the taxpayer by virtue of being a member of the Board of Directors of a resident company in Spain are liable for taxation in Spain Professional activities Corporate profits can only be liable...
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A review of non-financial incentives for health worker retention in east and southern Africa pot
... countries A number of summaries of 'best practice' strategies used in some of the countries are presented in the form of boxes in section of this paper The review was biased in favour of published ... DISCUSSION PAPER NO 44 Figure 1: Conceptual framework of non- financial incentives for retention of health workers Non- health determinants of health • • • • Health behaviours + lifestyle Personal ... the working environment, training capacity of the institutions, welfare of the workers and the quality of services rendered” (Tlou, 2006: 3) A review of non- financial incentives for health worker...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25