dna and biotechnology
Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"
Ngày tải lên :
25/10/2012, 11:18
... attached to DNA in a covalent manner,
and that both DNA- helix distorting and single strand
nick forms of DNA damage are important pathways
to apoptosis upon expression of NS1.
Materials and Methods ... apart. Nicking and binding to the DNA end
would not only create broken strands, but adducts
that would likely interrupt replication and activate
ATR-dependent DNA damage repair and apoptosis. ... NS1 and PARP.
Since PARP is active at the site of single strand DNA
breaks, and single-strand nicking is a known activity
of NS1 protein, it is likely that NS1 is nicking the cel-
lular DNA, ...
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Báo cáo y học: "HIV DNA and Dementia in Treatment-Naïve HIV-1-Infected Individuals in Bangkok, Thailand"
Ngày tải lên :
31/10/2012, 15:34
... Phatisawad, and Rapee Tri-
chavaroj; Sean Hill, David Troelstrup, Erik Anderson,
Andrew Williams, and Wichitra Apateerapong; and as
well as acknowledge the SEARCH participants for
their support and ... clades A, E,
and B; and demonstrated equivalent amplification of
the target gene.
Separation of PBMC Subsets and HIV DNA Analay-
sis.
Because HIV DNA is present in both lympho-
cytes and monocytes, ... in Bangkok, Thailand. Subjects with and without HAD were recruited and matched for age,
gender, education, and CD4 cell count. PBMC and cellular subsets were analyzed for HIV DNA using real-time...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
Ngày tải lên :
15/02/2014, 01:20
... ETS-1
(lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per-
formed with EWS (lanes 2, 4 and 6) and
32
P-labeled Htelo (lanes 3
and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). The
structures ... (lanes 2 and 4) and
32
P-labeled Htelo (lanes 1 and 2) or
rHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes
2 and 4) and
32
P-labeled rHtelo (lanes 1 and 2) or mut rHtelo (lanes
3 and ... mobility shift assay (EMSA)
with EWS and several G-quadruplex or single- or dou-
ble-stranded DNA and RNA. Here, we show that
EWS specifically targets G-quadruplex DNA and
RNA in vitro. We also determined...
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Tài liệu Intellectual Property and Biotechnology doc
Ngày tải lên :
19/02/2014, 05:20
... Ottawa: Canadian Biotechnology Advisory Committee, June; and Canadian
Biotechnology Advisory Committee (2003), Advisory Memorandum: Higher Life Forms
And ThePatent Act, Ottawa:Canadian Biotechnology ... <http://
web.archive.org/web/*/http://www.med.govt.nz/buslt/int_prop/genetic-material/cabinet/
implications/implications.pdf, November/; and New Zealand, Ministry of Health and
Ministry of Commerce (2004), Memorandum to Cabinet Policy Committee: Report Back
with Recommendations and Options for Addressing ... Gene,
London: Sidgwick and Jackson.
60. Kevles, D. (1994), ‘Ananda Chakrabarty wins a patent: biotechnology, law and society,
1972–1980’, Historical Studies in the Physical and Biological Sciences,...
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Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx
Ngày tải lên :
21/02/2014, 01:21
... concentration effect of Ami between
5 l
M
and 25 m
M
, and differences in the extent of formation
of form II between experiments (compare lanes 5 and 8 in
Fig.4Aandlanes 5and9 inFig.4BwiththoseinFig. 5A),
while ... of superhelical DNA to its linear form, and further,
to a continuum of short DNA fragments (Fig. 5C). The
relative persistence of form (II) and the stepwise character of
plasmid DNA degradation ... H
2
O
2
(part B), and
Ami + Cu(II) + H
2
O
2
(part C) for incubation times
between 5 and 95 min. As clearly seen, Ami + H
2
O
2
did
not produce form (III), and the ratio of forms (I) and (II)
remained...
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Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf
Ngày tải lên :
22/02/2014, 07:20
... para-
meters of the 24 DNA/ DNA and 41 RNA /DNA duplexes
at standard temperature (25 °C) and physiological
temperature (37 °C). Direct comparison shows that the
temperature-independent and temperature-dependent ... properties
for DNA/ DNA and RNA /DNA duplex formation
Peng Wu
1,
*, Shu-ichi Nakano
1
and Naoki Sugimoto
1,2
1
High Technology Research Center and
2
Department of Chemistry, Faculty of Science and Engineering, ... AND METHODS
Material preparations
DNA and RNA oligonucleotides were synthesized on a
solid support using the standard phosphoramidite method
with an Applied Biosystems Model 391 synthesizer and
purified...
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Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf
Ngày tải lên :
06/03/2014, 00:20
... factor (TF), the DnaK, DnaJ, GrpE system
and the GroEL–GroES pair. A comprehensive review
of the folding process transcends the aim of this paper
and can be found in [9,10] and references therein. ... derivative
spectrum and each peak can be assigned according to
its wavenumber. For instance, in water a-helices and
random coils absorb between 1660 and 1648 cm
)1
,
intramolecular b-sheets between 1640 and ... products due to translation errors and
misfolding are handled by the quality control system, composed of
refolding chaperones and proteases. The system is energetically
demanding (most processes are...
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FOOD LIPIDS Chemistry, Nutrition, and Biotechnology ppt
Ngày tải lên :
06/03/2014, 05:21
... parts: Chemistry and Properties;
Processing; Oxidation and Antioxidants; Nutrition; and Biotechnology and Biochemistry.
We are grateful to the readers and users of the previous editions and can only ... prostaglandins
and leukotrienes [14,15]. Examples of some of the possible conversions from 20:4v6 are shown in
Figures 1.5 and 1.6 [15]. The prostaglandins are called eicosanoids as a class and originate ... realize that there are 1-, 2-, and 3-series prostaglandins originating from
20:3v6, 20:4v6, and 20:5v3, respectively. The structures of the 1-, 2-, and 3-prostaglandins differ
∆-6-Desaturase
20:4ω3
18:4ω3
18:3ω3...
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Báo cáo khoa học: Human telomeric G-quadruplex: structures of DNA and RNA sequences ppt
Ngày tải lên :
06/03/2014, 09:22
... one strand and only
the 3Â-end G-tract of the other strand [30] (Fig. 3D).
The core of this structure, called the (3 + 1) core, has
three strands oriented in one direction and one strand
oriented ... consecutive G-tracts
are 5Â-syn-anti-anti-3Â and 5Â-syn-syn-anti-3Â for one
strand and 5Â-syn-syn-anti-3Â and 5Â-syn-anti-anti-3Â for
the other strand of the dimer; glycosidic conformations
of ... of DNA and
RNA sequences
Anh Tua
ˆ
n Phan
School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore
Introduction
Human telomeric DNA contains thousands of tandem
repeats...
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GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH pptx
Ngày tải lên :
06/03/2014, 23:20
... tail and 3’ blunt end, DnaB
enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000).
The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis. ... and spatially separate DNA unwinding
by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded).
It is known that when encountering a linear DNA with a 5’ tail and ...
interaction between Net1 and Sir2 on one hand and between Net1 and Fob1 on the other,
and loads Sir2 near the Ter sites. This process, as noted above, causes silencing of rDNA and
prevents unwanted...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
Ngày tải lên :
07/03/2014, 16:20
... NaH
2
PO
4
, and KI were obtained
either from Fluka (Buchs, Switzerland) or from Riedel-de-
Hae
ă
n (Seelze GmbH, Germany). dNTP, Pwo DNA poly-
merase, HindIII and EcoRI endonucleases, and T4 DNA
ligase ... CRPW85A–(cAMP)
2
and CRPW85A DNA complexes. Iodide and acrylamide
quenching studies were performed in Tris buffer, pH 7.9 at 20 °C. In the experiments with CRPW85A complexed to cAMP and DNA, the
concentration ... conformational changes induced by DNA and cAMP M. Tworzydło et al.
1106 FEBS Journal 272 (2005) 11031116 ê 2005 FEBS
CRPcAMPị
2
ỵ DNA !
k
on
k
off
DNACRPcAMPị
2
and which
k
obs
ẳ k
off
ỵ k
on
ẵCRPAEDANS1ị
with...
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DNA and chromosomes
Ngày tải lên :
13/03/2014, 16:39
... Assembly of the Core Histones
DNA and its Building
Antiparallel strands
Electron Microscope image of Drosophila polytene chromosome
Cell Nucleus, compartmentalized
DNA activity
Nuclear pores ... communication
Nuclear lamina and cytoskeleton
mechanically support the nucleus
DNA to Protein
Genome: the complete set of information in an organism’s DNA
Total length of DNA about 2 meters long ...
interphase chromosome
Human Chromosome
Complex of DNA and protein is called chromatin
44 homologous chromosomes and 2 sex chromosomes
Complementary DNA with different Dyes
The arrangement of the...
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Patents and New Product Development in the Pharmaceutical and Biotechnology Industries
Ngày tải lên :
13/03/2014, 21:59
... regulatory policy, and government
reimbursement controls.
6
The focus of this paper is on the role and impact of patents and
intellectual property protection in the discovery and development ... at a significant discount to the brand name firm, and this
discount grew larger as the number of generic competitors for a particular brand name
product expanded over time. For our 1984 to 1989 ... Tadmor, and Louis Lasagna, “Biopharmaceuticals
and Conventional Drugs Clinical Success Rates,” 10 BioTechnology (May 1992): 521-525; M.M. Struck,
“Biopharmaceutical R&D Success Rates and Development...
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Patenting of Biological Material and Biotechnology
Ngày tải lên :
13/03/2014, 21:59
... availability and scope of patent
protection on genes and genome-related technologies
is considered vital for the survival and success of the
biotechnology industry. Under US patent law, DNA
sequences ...
produced, processed or used’. This includes DNA-
related inventions, such as an isolated DNA fragment
and the gene it encodes or DNA sequence analysis
protocols and its software products. The definition ... globalization and TRIPS regulations is understanding
of intellectual property rights (IPR) laws of different countries in context of recent innovations in biotechnology and
bioinformatics. While biotechnology...
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Papaya biology and biotechnology
Ngày tải lên :
13/03/2014, 22:03
... researchers (Litz and Cono-
ver 1977, 1978a; Rajeevan and Pandey 1983, 1986; Mosella
and Iligaray 1985; Drew and Smith 1986; Drew 1988; Mon-
dal et al. 1990; Reuveni et al. 1990; Kataoka and Inoue
1991; ...
Reuveni and Schlesinger (1990) and Kataoka and Inoue
(1992) but stringent rooting conditions, seasonal factors,
and explant type affected rooting success (Kataoka and Ino-
ue 1992; Teo and Chan ...
Geographic distribution and nomenclature 48
BOTANY AND CULTIVATION 48
PESTS AND DISEASES 49
GENETICS, CONVENTIONAL AND MOLECULAR BREEDING 53
THE PLANT AND FRUIT: STRUCTURE, USES AND MEDICINAL PROPERTIES...
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