dissociation of embryonic kidney followed by re aggregation as a method for chimeric analysis
Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx
... manufacturer’s recommendations AK activity assays Kinetic analysis of AK activity was performed under empirically defined linear steady-state conditions Reactions were carried out at 37 °C in a final ... recombinant AK were included as standards for quantification Results were quantitated using NIH IMAGE software In vitro phosphorylation reactions All reactions were carried out at 30 °C in a final volume ... all cases, [32P]phosphate incorporation was assessed by SDS/ PAGE (15% acrylamide) and PhosphorImager analysis To calculate reaction stoichiometries, radiolabeled reaction products and radioactive...
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Báo cáo khoa học: "A PROLOG IMPLEMENTATION OF LEXICAL LANGUAGE FUNCTIONAL PROCESSING GRAMMAR SYSTEM AS A BASE FOR A NATURAL" pdf
... g~=~,~atical relations of a sentence But what about the logical relations? Recall that each clause has a unique head end that the functional features of each phrase are identified with those of ... ,S(i-l) 1~erefore the theory can accotmt for intersentential semantic relationships in the same way as for intrasentential ones ~ is the second requirement: a s~antic representation has to represent ... property that the functional features of each phrase are identified with those of its head The head category of a phrase is characterized by d~e assignment of the trivial ft~%ctional-equation and by...
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Báo cáo khoa học nông nghiệp " Nutrient recovery by rice crops as a treatment for aquaculture solid waste: crop yield, nutrient status and nutrient budgets " doc
... (Page et al 1982), plant analysis (Chapman and Pratt, 1961); water and wastes samples analysis followed methods for chemical analysis of water and wastes (MCAWW) EPA/600/4/79-020 revised March ... fishpond solid waste was applied before planting as was 50 % of the inorganic P and K The remaining P and K was applied at panicle initiation stage at 35 days after sowing (DAS) while N was split times ... Statistical analysis was completed with IRRISTAT software version 5.1 by applying a balanced one-way ANOVA Nutrient (N, P, K) balances were calculated following the approach of Dobermann and Fairhurst...
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Báo cáo khoa học: "An evaluation of decapitation as a method for selecting clonal Quercus petraea (Matt) Liebl with different branching intensities" ppt
... been analysed separately The effects of clones and treatments were investigated by analysis of variance As previous studies have shown that bud and branch numbers are related to shoot length (Harmer, ... 0.08-0.55 and 0.29-0.60 The numbers of branches present on each shoot are shown in figures and 6; as the only effect of temperature was a small 3-way interaction (table I), the data for both warm and ... overwintered original shoot are under weak apical control and many are able to form branches without decapitation; in contrast, buds on the first-flush are suppressed by strong apical dominance which...
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Báo cáo y học: "Direct Toll-like receptor 2 mediated co-stimulation of T cells in the mouse system as a basis for chronic inflammatory joint disease" ppsx
... obtained was used as a template for real-time quantitative polymerase chain reaction, which was performed with the LC FastStart DNA Master SYBR GreenI® (Roche Diagnostics, Mannheim, Germany) in a LightCycler® ... express toll-like receptors and are activated by lipopolysaccharide J Exp Med 2003, 197:403-411 Nagai Y, Akashi S, Nagafuku M, Ogata M, Iwakura Y, Akira S, Kitamura T, Kosugi A, Kimoto M, Miyake ... TLR-2, namely TLR-1 and TLR-6 [36-38,41], are also regulated rather markedly: a more than 10-fold decrease in TLR-1 was found in PMA/ ionomycin-treated freshly isolated B6 cells, as well as in...
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Báo cáo y học: "Sequencing the genome of the Burmese python (Python molurus bivittatus) as a model for studying extreme adaptations in snakes" ppsx
... genome that contains genic and near-genic regions that are assembled and annotated To provide a service to the broader research community, we have released a prepublication preliminary draft assembly ... There are a number of potential research areas that would probably be productive to pursue but are outside of the scope of the current plans of the project - these topics are therefore potential ... the families Viperidae (for example, rattlesnakes, and adders) and Elapidae (for example, coral snakes, cobras, and mambas) In addition to these lineages that contain commonly used model research...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... N Hattori and K Shiota methylation analysis Although RLGS requires a larger genomic sample than is necessary for microarray-based methods, it has advantages for analyzing genome-wide methylation ... provided by the re- methylating actions of Dnmt 3a ⁄ Dnmt3b (Fig 2) Dnmt 3a and Dnmt3b appear to function both as maintenance and as de novo methyltransferases in gene areas, and thus are crucial for ... Dnmt 3a and Dnmt3b are essential for de novo methylation and mammalian development Cell 99, 247–257 33 Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, Shimotohno K, Ishikawa...
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List of verbs phrases followed by gerunds infinitives
... someone of ing to warn someone against _ing to have a good reason for ing To Be + Adjective + Preposition Here are some combinations of the verb "to be" plus an adjective plus a preposition ... sau động từ dạng bị động: - He was known to be a great scientist "Full infinitive" dùng sau từ như: "first, last, second, next" - He was the first to get the prize - He was the last to hear about ... to participate in ing to take part in ing to think of ing to win by ing Verb + Object + Preposition Here are some verbs plus an object plus a preposition followed by a gerund:...
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Báo cáo y học: "Extension of the PNA world by functionalized PNA monomers eligible candidates for inverse Diels Alder Click Chemistyr"
... reaction rate, complete chemical reaction, lack of reverse reaction, chemical reaction in aqueous solution, under room temperature, no need for a catalyst) predetermines the DARinv as a suitable ... solvent was removed by the rotary evaporator and consecutively purified by a silica gel column As an eluent n-hexane and acetic ether were used at the ratio of 2:1 The purity was estimated by use of ... 4/Figure 4) after combination with facilitating features for the passage across biological membranes As illustrated in scheme 4/Figure 4, a cysteine is attached at the amino terminus of the PNA backbone...
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DIRECT TREATMENT OF POLLUTED RIVER WATER BY THE MULTI-SOIL-LAYERING METHOD
... system can be modified (optimized) and be applied for other type of wastewater or polluted water by changing of its material composition as examined by Luanmanee et al (2002) and Attanandana et al ... efficiency at a condition of high loading rate in this study In contrast to BOD and COD, SS removal rate was higher at a loading rate of m3 m-2 day-1 than at that of m3 m-2 day-1 as shown in the results ... the results showed better performance (Unno et al., 2003) REFERENCES Standard Method for the Examination of Water and Wastewater (1995) 19th edn, American Public Health Association/American Water...
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Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
... must vanish In the model we have assumed a linear decrease of the conversion probabilities: VA ¼ VA0 À nðSVAÁDtÞ for VA > AZ ¼ AZ0 À nðSAZÁDtÞ for AZ > where VA0 and AZ0 are the initial values of ... monogalactosyldiacylglycerol reversed hexagonal phase was measured by PCS analysis The 10 mW He-Ne laser (633 nm) was used as a light source The selected angle was 90°, the viscosity was 0.890 centipoise and refractive ... liposomes, more violaxanthin was deepoxidated and more zeaxanthin was formed in the monogalactosyldiacylglycerol system at the plateau stage of the reaction The same was found at °C, although at this...
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Báo cáo khoa học: Quantitative assessment of the glyoxalase pathway in Leishmania infantum as a therapeutic target by modelling and computer simulation pot
... concentration time courses So far, this analysis of the glyoxalase pathway has only been performed in yeast [20] The glyoxalase pathway enzymes catalyse irreversible reactions and can be considered as ... http://jjj.biochem.sun ac.za/database/silva/index.html free of charge Results and Discussion The potential of the glyoxalase system as a possible therapeutic target relies on its role as the main catabolic pathway ... this parasite by initial rate analysis Using the methylglyoxal glutathione hemithioacetal as substrate, the kinetic parameters for L infantum glyoxalase I, were a Km of 1.85 0.35 mm and a V of...
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Báo cáo khoa học: Induction of PAI-1 expression by tumor necrosis factor a in endothelial cells is mediated by its responsive element located in the 4G/5G site ppt
... were treated with TNFa (50 ngÆmL)1) for 24 h and the cells harvested 12 h later Luciferase activity was determined by the Dual-Luciferase assay kit Data are shown as the mean of three separate ... analyzed by real-time PCR All experiments were performed at least times with reproducible results and data are shown as mean ± SD (E, F) Changes in PAI-1 expression as measured at the level of PAI-1 ... was the response of other acute-phase reactants, which implies that the increases in these reactants are secondary to the increase in PAI-1 [27] Our data show that activation of endothelial cells...
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Báo cáo y học: "A comparative study of the inhibitory effects of interleukin-1 receptor antagonist following administration as a recombinant protein or by gene transfer." pps
... situations, such as that reported in Figs and 5, the importance of maintaining the local level of IL-1Ra became dramatically apparent, as were the advantages of gene transfer as a means of protein ... disease Indeed, IL-1Ra gene therapy has demonstrated impressive efficacy in animal models of RA and OA, and a phase I human trial has recently confirmed that the human IL-1Ra cDNA can be safely ... the cellular level, the advantage of gene transfer as a means of drug delivery arises from the sustained availability of IL-1Ra that this method permits Materials and method Materials Ham’s F12...
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báo cáo khoa học: " Inflammatory pseudotumors of the kidney and the lung presenting as immunoglobulin G4related disease: a case report" pps
... were performed, leading to the diagnosis of IgG4-related disease There are some reports of patients who presented with a renal mass, and thus underwent a nephrectomy as the mass was mistaken for ... criteria of autoimmune pancreatitis: revised proposal J Gastroenterol 2006, 41(7):626-631 Kamisawa T, Okazaki K, Kawa S, Shimosegawa T, Tanaka M, Research Committee for Intractable Pancreatic Disease ... IgG4-related systemic diseases, so we based our treatment on the Japanese consensus guidelines for the management of autoimmune pancreatitis, as recommended by the Japan Pancreas Society Treatment...
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Báo cáo y học: " Fibrolipoma of the lip treated by diode laser surgery: A case report" pps
... Journal of Medical Case Reports 2008, 2:301 Figure Clinical appearance of fibrolipoma Clinical appearance of fibrolipoma This lesion usually presents as an asymptomatic swelling of soft consistency, ... the patient, with no inaesthetic alterations of the adjacent tissues Fibrolipoma is a benign tumour that rarely occurs in the oral and maxillofacial region, and is classified as a variant of conventional ... lipoma of the oral cavity: report of a case J Oral Pathol Med 1994, 23:237-239 Furlong MA, Fanburg-Smith JC, Childers EL: Lipoma of the oral and maxillofacial region: Site and subclassification of...
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Báo cáo y học: "Urine neutrophil gelatinase-associated lipocalin is an early marker of acute kidney injury in critically ill children: a prospective cohort study" pot
... (105.0) Mean uNGALa Peak uNGALa Values are expressed as mean ± standard deviation/median (interquartile range) aMean and peak urine neutrophil gelatinase-associated lipocalin (uNGAL) concentrations ... (1.1) Peak uNGALa Mean uNGALa,b aPeak Values are expressed as mean ± standard deviation/median (interquartile range) and mean urine neutrophil gelatinase-associated lipocalin (uNGAL) concentrations ... design and data interpretation Qing Ma participated in the urinary NGAL measurements PD performed uNGAL measurements and participated in data interpretation CRP participated in the statistical analysis...
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Báo cáo y học: "Review Native aggregation as a cause of origin of temporary cellular structures needed for all forms of cellular activity, signaling and transformations" ppsx
... phase was called the phase of excitation and damage by Nasonov's school Substantial changes in the cell in this phase are remarkably reminiscent of denaturation of isolated proteins; therefore, ... genetic regulation of apoptosis can be a source of the signal that leads, as a result of native aggregation, to the appearance of a structure that will trigger the whole cascade of reactions necessary ... aggregation is completely reversible, and the structures appearing in the course of native aggregation are temporary and are disassembled as soon as they cease to be necessary Native aggregation...
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A study on the structure of the speech “ I have a dream” by Martin Luther King A systemic functional grammar analysis
... systemic functional grammar analysis based on Halliday’s functional grammar as the theoretical framework 1.2 Aims of the study In carrying out the research, the writer aims to: Illustrate the key ... with a number of grammar exercises in schooling as well as being the teacher for many grammar classes, I am deeply interested in improving the language proficiency along with the structure accuracy ... summarizes the outcome of the research and suggests some further implications for language teaching and learning References Alexander, T (2004) Basic Concepts of Discourse Analysis Available : www.grin.com...
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Use of human altered habitats by bull sharks in a florida nursery area
... temperatures matched ambient lagoon temperatures), we defined the area within km of each outfall as being altered Areas within 20 m of boat ramps, causeways, and dredged channels were also defined as ... to altered areas has been tied to increased levels of mercury bioaccumulation and a variety of toxic effects (Adams et al 2003; Adams and Paperno 2012; Mull et al 2012) Loss of natural habitats ... Plant (Figure 3) Shark B4 demonstrated selection for the boat marina area of Crane Creek (Figure 4) Finally, shark B8 demonstrated selection for the causeway/bridge area and Intracoastal Waterway...
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