... digesting the fragment with HindIII and Bgl II andthe plasmid with HindIII and Bam HI. The fragment was ligated into the plasmid andthe integrity of the insertion was validated by digesting the plasmidconstruct ... atpositions 597, 641, 667, and 691.To construct the GST fusions ofthe tandem SH2 domains of each SHP-1 and SHP-2, the sequences corresponding toamino acids 2–232 of each SHP-1 and SHP-2 wereamplified ... with Eco RI and Bbr PI, then gel purified(< 820 bp). The insert was then ligated into the pSPORTvector andthe resulting full-length siglec-10 clone wasdesignated 995-2 andthe sequence...
... consists of three parts: the blade, the thin expanded portion; the petiole, the leafstalk; andthe stipules, a pair of small blades at the base of the petiole. The petiole is often very short and ... ground at the base ofthe tree; move the arm and pencil upward till the thumb is in line with the paper, and note where the end ofthe pencil comes on the tree. Again move the pencil till the thumb ... small book the barest outline ofthe parts of flowers and fruit andoftheir uses can be given. Fig. 9. Flowers.—Parts. The flowers ofthe Cherry or Apple will show the four kinds of organs...