discuss the role of a security manager in an organization
Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
Ngày tải lên :
22/02/2014, 04:20
... Exploring the role of a glycine cluster in cold adaptation
of an alkaline phosphatase
Konstantinos Mavromatis
1,
*, Iason Tsigos
2,
*, Maria Tzanodaskalaki
2
, Michael Kokkinidis
1,3
and Vassilis ... the aromatic ring of Tyr269, and these
unfavorable interactions could lead to a decrease of local
flexibility and an increased E
a
value.
The validity of the above interpretation was further
reinforced ... exhibits an E
a
almost the same as in the case
ofthenativeenzyme(Table1).
Thermal inactivation of mutant and wild-type enzymes
In order to investigate the effects of mutations on the
stability of psychrophilic...
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- 488
- 0
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... state may be
maintained mainly by key amino acids. In this study, two point-mutated
proteins each with a single base substitution [alanine for tryptophan
(W14 0A) and alanine for lysine (K13 3A) ] and ... the mutants without tryptophan at 140
(W14 0A and W140O) was much lower than both the
wild-type protein and mutants E142O and K13 3A.
Thermal analysis of protein unfolding
The DSC curves of the wild-type ... investigated. Two point-mutated proteins, with a single
base substitution of alanine for tryptophan (W14 0A)
and alanine for lysine (K13 3A) , and two truncated
fragment proteins, with fragments...
- 7
- 551
- 0
A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx
Ngày tải lên :
22/03/2014, 18:20
... rupture of a pair of hydrogen
bonds in the A and B b-strands near the amino terminus of
the protein domain causes an initial extension of the protein,
before the unfolding transition state is reached.
[53]
The ... be-
tween the first amino acid of strand A (Y9) and the last amino acid of
strand G (K87) . The elongation of the x(Y9)Àx(87) distance up to the transi-
tion state is defined as the distance Dx
A ÀG
. ... b-strands. In Figure 5 B, we define the pulling coor-
dinate for the A and G b-strands as the distance between the
first amino acid of strand A (Y9) and the last amino acid of
strand G (K87)....
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- 553
- 0
Tài liệu The Role of Public Regional Universities in Community and Economic Development doc
Ngày tải lên :
21/02/2014, 01:20
... average, command a significantly higher salary ($42,000
annually vs. $28,000 in average annual salary)
Preserving Value
Continuing education is important to preserving the market value
of specialized ... knowledge and forming intellectual capital
Nearly two-thirds of National Association of State Universities and
Land-Grant Colleges (NASULGC) have research parks or business
incubators (PASSHE has ... Programs
and Initiatives
Business accelerators and/or entrepreneurial initiatives
Linking teaching and research to business opportunities
Collaboration with local business and industry
Small Business...
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- 654
- 0
Báo cáo khoa học: The role of interface framework residues in determining antibody VH ⁄ VL interaction strength and antigen-binding affinity pptx
Ngày tải lên :
07/03/2014, 12:20
... (5Â-GGCAACAACCTACACTGGTATCA
ACAAAAAYMGSRCRAATCTCCTCRGCTCCTGRTCW
AKTATGCTTCCCAGTCCATCTCT-3Â) and g7EcoFor
(5Â-AGTGAATTCTCATCTTTGACCCCCAGCGATTAT
ACCAA-3Â). As a linker to connect these two, a DNA
encoding ... To insert tHP downstream of the ORFs, tHP4 and
tHP7 (5Â-AATTGGTACCCGATAAAAGCGGCTTCCTG
AC-3Â), as well as tHP2 and tHP8 (5Â-AATTGAGG
TGGGCTGCAAAACAAAACGGCCT-3Â), were annealed
and ligated to the ... types of mutants were also observed.
SPR analysis of antigen-binding affinity
For the quantitative evaluation of the H39 mutation,
kinetic analysis for antigen–Fv interaction of the
wild-type and...
- 11
- 462
- 0
Báo cáo khoa học: "The Role of Lexico-Semantic Feedback in Open-Domain Textual Question-Answering" ppt
Ngày tải lên :
08/03/2014, 05:20
... semantic
The Role of Lexico-Semantic Feedback in Open-Domain Textual
Question-Answering
Sanda Harabagiu, Dan Moldovan
Marius Pasáca, Rada Mihalcea, Mihai Surdeanu,
R
azvan Bunescu, Roxana G
rju, ... if (a) a vol-
cano IS -A mountain; (b) lava IS-PART of vol-
cano, and moreover it is a part coming from the
inside; and (c) fragments of lava have all the prop-
erties of lava, the following question ... WordNet: A Lexical Database. Commu-
nication of the ACM, vol 38: No11, pages 39–41, Novem-
ber 1995.
Dan Moldovan, Sanda Harabagiu, Marius Pasáca, Rada
Mihalcea, Richard Goodrum, Roxana Grju and Vasile
Rus....
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- 0
Báo cáo Y học: The presence of a helix breaker in the hydrophobic core of signal sequences of secretory proteins prevents recognition by the signal-recognition particle in Escherichia coli doc
Ngày tải lên :
08/03/2014, 09:20
... revealed that the G-10L mutant
protein is routed to the SecYEG translocon via the SRP
pathway, the targeting pathway that is exploited by integral
inner-membrane proteins. Together, these data indicate
that ... Bioscience (Maarsen, the Netherlands).
Bacterial strains
The E. coli K-12strainsusedinthisstudyarelistedin
Table 1. Strains CE1514 and CE1515 were obtained by P1
transduction using strain CE1224 as the ... understandable, as the SecB-binding
sites, which are located in the mature domain of the PhoE
precursor [3], are not affected in the G-10L mutant
precursor. Thus, in the absence of SRP, SecB can target
the...
- 8
- 546
- 0
Báo cáo " THE ROLE OF GIFT RECIPIENT PERCEPTION IN CHANGING BRAND ATTITUDES AND GIVER - RECIPIENT RELATIONSHIP " potx
Ngày tải lên :
14/03/2014, 14:20
... instrumental in
maintaining social ties and serves as a
mean of symbolic communication in social
relationship.
Most of the researches before and after
the appearance of Sherry’s model in 1983
can ...
brand attitude. It is the primitive
assumption of balance theory that
unbalanced triads tend toward balance.
Although balance theory help to
explain the change in recipient’s post
brand attitudes ... have positive brand
attitudes, then the triad is said to be
unbalance. In this example, balance could
be attained if the receiver changes to
dislike the giver or having post favorable
brand...
- 10
- 482
- 0
Báo cáo khoa học: The antagonistic effect of hydroxyl radical on the development of a hypersensitive response in tobacco pot
Ngày tải lên :
15/03/2014, 00:20
... suppres-
sion. After in ltration, the treated plants were placed in the light,
and the HR extent was evaluated by the ion leakage ratio at 12
and 48 h after in ltration. Mean values ± standard error of three
replicates ... Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agricuture of R. P. China, Department of
Plant Pathology, College of Plant Protection, Nanjing Agricultural ... with ABA or H
2
O,
ParA1 and the spin trapping assay reagent were coinfiltrat-
ed into the leaves, and the plants were placed under light.
After 6 h, the levels of OH
ã
in the leaf tissues obtained
from...
- 15
- 479
- 0
The Role of Savings and Wealth in Reducing ―Wilt‖ between Expectations and College Attendance pdf
Ngày tải lên :
15/03/2014, 10:20
... savings may
increase the means to afford college.
The other effect is indirect and mainly attitudinal. Having savings over a period of years may raise a
young person’s educational expectations ... to increase youth account ownership and savings may play an important
role in helping to restore the American Dream of attending college. Because this research finding
has simple, doable, and ... importance of youth
savings for understanding college attendance.
Conclusion
Findings from this study suggest that factors other than desire and ability play an important role in
determining...
- 22
- 515
- 0
Báo cáo khoa học: The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism pdf
Ngày tải lên :
16/03/2014, 14:20
... and the nuclear ⁄ cytoplasmic ratio was analysed from
the Gray images using Lucia G ⁄ F Analysis Software [6].
Data analysis
Results are presented as mean ± SE for the number of
hepatocyte preparations ... were washed in NaCl ⁄ P
i
, fixed in
4% (w ⁄ v) paraformaldehyde in NaCl ⁄ P
i
and stained for glu-
cokinase with a rabbit IgG against human glucokinase:318–
405 (H88, sc-7908; Santa Cruz) and fluorescein ... h and
3 h incubations, and the cell content of Glc6P and the activ-
ity of phosphorylase were also similar after 1 h and 3 h.
Metabolite and enzyme assays
Glc6P and Fru2,6P
2
were determined as...
- 11
- 503
- 0
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