directed in vitro evolution of reporter genes based on semi rational design and high throughput screening
Báo cáo hóa học: " Research Article Fast Signal Recovery in the Presence of Mutual Coupling Based on New 2-D Direct Data Domain Approach" pdf
... method,” International Journal of Antennas and Propagation, vol 2010, Article ID 373061, pages, 2010 [6] T Svantesson, “Modeling and estimation of mutual coupling in a uniform linear array of dipoles,” ... studied for recovering of the signal in the presence of mutual coupling and driving a new formulation to recover the signal in the presence of MC Without using the moment of method and impedance matrix ... coupling on the performance of adaptive array,” IEEE Transactions on Antennas and Propagation, vol 31, no 5, pp 785–791, 1983 [3] B Friedlander and A J Weiss, “Direction finding in the presence of...
- 9
- 415
- 0
Developing and evaluating in vitro effect of pegylated liposomal doxorubicin on human cancer cells
... doxorubicin is capable of releasing prolonged and increasing circulation time in blood In previous reports, Ye et al showed the cytotoxicity of different liposome products containing doxorubicin ranged ... IC50 values (concentration resulting in 50% growth inhibition) of doxoburicin were graphically calculated from concentration-effect curves, considering the optical density of the control well as100% ... acquires in a particular medium The destination of the liposomes in vivo could be predicted by using the information of the zeta potential of a liposome preparation The high absolute value of zeta...
- 5
- 277
- 0
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... upon activation and it translocates to the nucleus where it induces the transcription of a large number of target genes encoding regulators of immune and inammatory responses and also genes involved ... determined from the global t The constant a is the ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding ... population of 10 000 A549 cells, which is consistent with synchronous behavior Secondly, we present in vitro kinetic measurements of IKK2 protein kinase We demonstrate that the assay conditions can...
- 13
- 475
- 0
Báo cáo y học: " Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution" docx
... additional mutations in the U3 and TAR region upon long-term culturing SIV-rtTA acquires additional mutations in the U3 and TAR region upon long-term culturing SIV-rtTA was cultured with dox in ... vaccine virus upon vaccination is due to the persistence of the virus and ongoing lowlevel replication The error-prone viral replication machinery can facilitate the generation and accumulation of ... substitutions not restore Tat binding Mutations in U3 and TAR not affect promoter activity In addition to the mutations in TAR, SIV-rtTA acquired mutations in the U3 region upon long-term culturing...
- 14
- 210
- 0
Báo cáo y học: "New genes in the evolution of the neural crest differentiation program" pot
... point, we focused on the collection of neural crest 'new genes' to gain insight into their molecular nature and function Emergence of ligands for neural crest lineage specification information ... role during vertebrate evolution in general and neural crest evolution in particular Individual analysis of the function of these peptides during the development of the neural crest demonstrates ... Calcitonin/calcitonin-related polypeptide, alpha Cocaine and amphetamine regulated transcript Endothelin Endothelin Formin Glial cell line derived neurotrophic factor Gonadotropin releasing hormone...
- 17
- 394
- 0
Báo cáo y học: "The role of RNA folding free energy in the evolution of the polymerase genes of the influenza A virus" potx
... showing various plots of folding energies (referenced in the main text) and one table listing the folding energies of vaccine strains WHO and CDC use against H5 influenza 19 20 Click of folding ... the polymerase and NP genes, and independent of the codon usage bias In addition, human influenza A strains have increasingly higher folding energies over time (within a certain range), especially ... strains compared to the avian strains at these positions Instead, we found that degenerate positions in the avian strains contained bases with higher bonding potential than in the human strains...
- 10
- 321
- 0
Analysis, sequencing and in vitro expression of PCR products
... are based on DNA separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading ... accumulation of one strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99 Template DNA PCR with one biotinylated primer B Biotinylated ... using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be expressed and...
- 24
- 494
- 0
Tài liệu “Measuring customer satisfaction in the context of a project-based organization” docx
... sampling (11/37) procedures, the quality of survey data and instruments, non-response problems and problems relating to reporting and interpretation of the results of the survey (Lin & Jones, ... each key individual’s satisfaction or dissatisfaction and the underlying reasons The interviewing skills of the person conducting the CS interviews are essential in ensuring the validity and reliability ... rely on generalized and project context independent information in their decision-making H13: Middle and lower levels of management are more likely to use interview, CIT and SIT based information...
- 37
- 1,063
- 0
Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... linked hairpins in the membrane [10] Importantly, the ion-binding site is not conserved in hairpin one If one assumes the same number of transmembrane helices in V0 and F0, the rotor of eukaryal ... missing one ion binding site (in hairpin two) The genes atpE2 and atpE3 encode two identical kDa subunits with one ion-binding site each The three genes are encoded in the same operon and their products ... and the densities correspond to the N- and C-terminal helices of the c ring, respectively The C-terminal helices show a clear handedness, and two of the rings face in the opposite direction in...
- 9
- 773
- 0
Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... and doxorubicin were present, similar inhibition was found at experimental concentrations (data not shown) In vitro studies show that single-stranded tracts containing (CTG)n repeats have a higher ... was right-handed, hence in the P conformation [14] In order to observe the conformational transitions of DNA directly and to eliminate drug interference, the CD spectra of native DNA and altered ... simple groove binding and ⁄ or electrostatic interaction that showed fewer or no perturbations on the base stacking and helicity bands [34], ruling out the possibility of conformational change FEBS...
- 12
- 473
- 0
Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... an increase in p21waf1 expression, loss of CDK function, and the generation of cells with senescence characteristics In the first part of the investigation, we determined the effect of continuous ... studies reporting CDO-induced inhibition of cyclin/CDK operation and cell proliferation [18,19], which were in concordance with our results, and highlighted a modulatory effect of CDO on cell cycle ... progression However, in contrast with our results, these studies showed a reduction in cyclin D and E expression Disappointingly, the specific interactions between CDKs and cyclins were not assessed, and...
- 9
- 331
- 0
Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring inhibition of the endonuclease reaction [36] In ... nucleotides (nt) in length and contained 311 nt of intron sequence, 112 nt of 5¢ exon and 209 nt of 3¢ exon sequence HindIII-derived pre-RNAs contained a shorter 3¢ exon, 29 nt in length Endonuclease ... binding assay in TK8 buffer with nM I-AniI and varying concentrations of end-labeled pCOBLE or uniformly labeled AnCOB pre-RNA RNA binding reactions contained mM MgCl2 and DNA binding reactions...
- 12
- 483
- 0
Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
... kDa protein with two hairpins [20] Most important, the ion-binding site is not conserved in hairpin If one assumes the same number of hairpins in V0 and F0, the rotor of V1V0ATPases has only half ... a hairpin and to have one ion-binding site In contrast, subunit c1 is similar to the c subunits of V1V0-ATPases and predicted to have four transmembrane helices with only one ion-binding site ... washed once with buffer B, and resuspended in 60 mL of buffer B luminol), and solution B (10 mL of dimethylsulfoxide containing 11 mg of p-hydroxycoumaric acid)] Blot membranes were incubated in...
- 8
- 486
- 0
Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... (1991) Initiation and progression in mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate Bone Mineral 14, 27–40 32 Coc, M.R., Summers, T.A., Parson, ... h incubation of one of the eight phosphate substrates (12.5 mM), in mineralization medium containing calcium (24 mM) in the presence of purified chick embryo GPI-bALP X-ray spectra corresponding ... the interpretation of IR spectra of mineral deposits Distinct mineralization steps were observed during the GPI-bALPinduced hydrolysis of b-GP At incubation times in the range 1.5–3 h, two bands...
- 9
- 367
- 0
Efficient site directed in vitro mutagenesis using ampicillin selection potx
... on five of the mini-preps Sequence data indicated that four of the five clones contained both of the mutations Restriction analysis of the ten clones indicated that eight of the ten clones contained ... method gave high efficiency mutagenesis in a restoration of function mutation at the Pst I site in the Testing pSELECT-1 with an Insert In order to test the functioning of pSELECT- with an insert, ... then cloned into the Hind III site of the vector Each colony found to grow on medium containing both tetracycline and chloramphenicol was found to contain the insert oriented for expression by...
- 5
- 131
- 0
Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... membrane types (Fig 1A,B) in the presence of 0.2 mM of ubiquinone and, to some extent, of dPQ, duroquinone or vitamin K1 (phylloquinone) In contrast, addition of benzoquinone did not lead to such ... decyl-ubiquinone (dUQ), duroquinone (DQ), vitamin K1 (VK1) or benzoquinone (BQ) Sequential addition of KCN (inhibitor of the E coli cytochrome b-dependent O2 consumption; mM final concentration) and ... PTOX-containing membranes, O2 consumption rates decreased to background levels in the assays containing ubiquinone, vitamin K1 or benzoquinone (Fig 1A), as they did without quinone addition (not...
- 8
- 466
- 0
in vitro release of ketoprofen from proprietary and extemporaneously manufactured gels
... Permeation will depend on the degree of ionisation and how ionisation influences the solubility in the applied phase and its partition into the skin One of the problems involved in interpreting permeation ... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ... formulations using Carbopol® polymers and study the effect of polymer type, pH, loading concentration and solvent composition on the in vitro release of ketoprofen To compare and contrast the in vitro...
- 222
- 693
- 0
báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals [29,30] The number of DCs used for the generation of fusions was 1–2 × 106 in ... vaccination with fusions of the patient-derived DCs and autologous HCC cells was started after month of operation (with following informed consent and approval of clinical protocols by our Institutional ... Journal of Translational Medicine 2008, 6:51 http://www.translational-medicine.com/content/6/1/51 Conclusion: The current study is one of the first demonstrating the induction of antigen-specific...
- 19
- 459
- 0
Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral ... noticed indicating that activated bovine PBMC are not permissive to MYXV infection Conclusion C D Figure Electron2microscopy observations of MYXV BT infected cells Electron microscopy observations of ... cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing LacZ gene...
- 5
- 469
- 0
Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
... through binding of CCL2 to its corresponding chemokine receptor CCR2 on the T cells This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the ... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining of ... adding T cells Day of harvesting of reconstruct cultures was based on optimum constriction of the collagen gel of reconstruct cultures The ratio of apoptotic tumor cells was determined by counting...
- 11
- 370
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25