direct methods for the detection of reactive oxygen species in human semen samples
Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf
... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... microscope Stretching of these metacercariae was done by hot formalin for two minutes Then, they were kept in Eppendorf tubes with neutral formalin (4%) Diagnosis of parasitic infections * Diagnosis ... saline pH 7.0) The internal organs were exposed after a vertical incision was made from the anal opening to the Before any dissection, the exterior of the fish was observed under the dissecting...
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báo cáo hóa học:" Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach" docx
... 8.2) and the mixture was incubated at 37°C for 15 in the presence of mM H2O2 and mM FeCl2 At the end of the incubation, mL of thiobarbituric acid 0.375% in 0.2 N HCl was added to the incubation ... analyses were performed using the GraphPad Prism software (version 5.0 for Windows; GraphPad Software Inc., La Jolla, CA) Quantification of reactive oxygen species To measure the amount of free radicals ... of reactive oxygen and nitrogen species In vitro generation of reactive oxygen and nitrogen species by neutrophils in contact with PM2.5 A In vitro production of reactive oxygen and nitrogen species...
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Cocoa Processing Methods for the Production of High Quality Cocoa in Vietnam ppt
... assistance in providing information regarding the cocoa industry in Vietnam and in the establishment of solar driers and fermenting boxes at their demonstration farm sites in Ben Tre Province HARVESTING ... darken in colour during drying as the majority of browning reactions will occur during this process DRYING OF COCOA Drying of cocoa is an important step in cocoa processing as some of the reactions ... on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two hours, prior to placement in boxes, on fermentation temperatures 7: The effect of spreading of...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... proteinase K pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled...
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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc
... SBEIIa-D(A201T) the amino acid change induced by the EMS treatment is located in the region of the N-terminal domain of the glycogen branching enzyme family, reported to be essential for the size of the ... product showed the inclusion of the intron V, whereas the smaller one was found to contain a deletion of the first seven nucleotides of exon VI The presence of the intron V in the longer transcript ... mutations in the genome of the target organism, although in polyploids the presence of multiple copies of a gene has represented a major limitation in the detection of interesting phenotypes for valuable...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... unitsÆmg protein)1, with unit of SOD activity being defined as the amount of proteins causing half-maximal inhibition of the rate of NBT reduction GST activity was assayed in the supernatant of cell ... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in ... normally in the absence of mtDNA These findings are in line with results found in hepatoma-derived Hep1q0 cells [16] ROS When DCF signal was assessed as a direct index of ROS, all of the q0 cells...
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Statistical methods for the detection and analyses of structural variants in the human genome
... 2009) 22 The idea is that where the location of the unmapped read may span the breakpoint of the CNV SR analysis has the advantage of being able to pinpoint the location of the breakpoints Assembly-based ... STATISTICAL METHODS FOR THE DETECTION AND ANALYSES OF STRUCTURAL VARIANTS IN THE HUMAN GENOME TEO SHU MEI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... that the costs associated with down-handling, storing and analysis of the data could be more than the production of the data There is still a need for the development of new statistical/bioinformatics...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... stenosis from the individual centers included in the meta-analysis The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile ... measured by the MCG device The resulting mathematically integrated patterns of the abnormal indexes are then compared for their degree of abnormality to the abnormal index patterns in the reference ... according to whether the patients had prior revascularization The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated ... recommend the Ames test using these sensitive strains (especially YG1041 and/or YG1042) in combination with the standard tester strains (TA98 and TA100 series), in order to detect the mutagenicity of ... ogttST::Cmr) strain (deficient in O6-methylguanine DNA methyltransferase) sensitive to alkylating chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences...
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Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge
... of 95 ml) The A280 to A260 ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... shaking, absorbance at 660 nm (A660) was measured for min, and the relative A660 against the control test was calculated every minute using the following formula: The relative A660 = A660 of flocculating ... respectively); in contrast, the steaming-extracted polymer included a larger amount of saccharides (24.6%) compared to the other methods The SDS-extracted polymer included a small amount of material other...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... with MDP (100 lgÆmL)1) for h results in significant increase in UCP2 expression (3.6-fold, P < 0.05) On the other hand, analysis of the kinetics of induction of UCP2 protein in MDP-treated macrophages ... [28] These findings agree with the hypothesis [29] that an increase in the mitochondrial membrane potential would slow the transport of electrons through the respiratory chain, increasing the time ... induction of in ammatory responses [22] Despite a long-standing interest in the field of muramyl peptides, the impact of these molecules at the mitochondrial level has not yet been examined Recently the...
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Critical evaluation of diagnostic aids for the detection of oral cancer docx
... carcinomas Of the 50 clinically visible lesions, 20 stained positively with toluidine blue and of these were carcinomas One of the clinically detected carcinomas was dye-negative Therefore, out of ... clinical examination and case-finding, even in primary care In experienced hands toluidine blue staining may be useful in the evaluation of oral lesions and as an adjunct in the surveillance of ... respectively One of the weaknesses of this study is the small sample size In addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology...
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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx
... licensed to and installed on the computer performing the editing In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy The ISO Central Secretariat ... information concerning the strain(s) and whether it is an outbreak or in food 10 Expression of results In accordance with the results of the interpretation, indicate the presence or absence of ... this culture for examination for the H-antigens, proceeding according to 9.5.4.2, but using one drop of the anti-H serum (5.3) instead of the saline solution If agglutination occurs, the reaction...
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ag doped wo3-based powder sensor for the detection of no gas in air
... patterns (the lack of their long-range order and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar ... when the temperature of the Ag catalyst is increased to 250 8C, the sensitivity of the WO3 sensor kept at 150 8C 71 increased by three times Without heating the Ag catalyst or bypassing the gas ... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18]...
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Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II ppt
... to the gene encoding the PsbQ¢ extrinsic protein, which is required for effective binding of the PsbV and PsbU proteins in the red lineage, and to the gene encoding the PsbQ extrinsic protein, ... plant species Among the extrinsic proteins, the 33 kDa protein (PsbO) which plays an important role in maintaining the stability and activity of the manganese cluster is present in all of the oxygenic ... positions in the evolution of oxygenic photosynthetic organisms In this study, we examined the distribution of the extrinsic proteins in these organisms using antibodies raised against PsbV,...
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nmr methods for the investigation of structure and transport
... spins By linearity of the Fourier transform the direct relation between the peak integral and the number of spins of the corresponding chemical group holds for the spectrum of superposed lines ... real, the oscillating imaginary part of the sum in general leads to a small imaginary part of Qn The maximum of the real part of the sum is N times higher than the amplitude of the oscillating ... organized the continuation of the research unit after the DFG funding as well as Prof Kasper, Prof Kind, Prof Nirschl, and Prof Elsner Prof Nirschl, Prof Kind, Prof Wilhelm, and Prof Elsner contributed...
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direct methods for stability analysis of electric power systems theoretical foundation, bcu methodologies, and applications
... for the direct methods, including the closest UEP method, the PEBS method, and the controlling UEP method in Chapters through 13 The central theme of the theoretical foundation is knowledge of ... nonlinear in nature Their nonlinear behaviors are difficult to predict due to (1) the extraordinary size of the systems, (2) the nonlinearity in the systems, (3) the dynamic interactions within the ... prefault SEP and the system state at the time of fault clearing using the step-by-step numerical integration of the fault-on system In the second step, the direct methods directly determine, without...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... range of the assay the MfaRV2 and 72% (r2 = 0.929) for the PcyRV2, however, the low levels of virus in these samples made it difficult to accurately determine the efficiencies, as indicated by the ... using the sum of the errors of the viral and OSM copy number determinations, as described in Materials and Methods * These results, while positive for both duplicates, were outside of the linear...
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báo cáo hóa học: " The evolution of methods for the capture of human movement leading to markerless motion capture for biomechanical applications" pptx
... Determination of the moments of inertia of the human body and its limbs Berlin: Springer-Verlag; 1988 Eberhart H, Inman V: Fundamental studies of human locomotion and other information relating ... to minimize skin movement artifact by optimal weighting of the markers according to their degree of deformation Another extension of the basic PCT corrects for error induced by segment deformation ... skin The movement of the markers is typically used to infer the underlying relative movement between two adjacent segments (e.g knee joint) with the goal of precisely defining the movement of the...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... range of the assay the MfaRV2 and 72% (r2 = 0.929) for the PcyRV2, however, the low levels of virus in these samples made it difficult to accurately determine the efficiencies, as indicated by the ... using the sum of the errors of the viral and OSM copy number determinations, as described in Materials and Methods * These results, while positive for both duplicates, were outside of the linear...
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